• Title/Summary/Keyword: Rearing media

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Selection of Rearing Media, Proper Temperature and Period for Storage of Sterile Lucilia sericata (Diptera: Calliphoridae) Eggs and Larvae (무균 구리금파리 유충 생산용 배지의 선발과 알 및 유충의 저장을 위한 온도 및 기간)

  • Jang, Sin-Ae;Yun, Ji-Eun;Park, Chung-Gyoo
    • Korean journal of applied entomology
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    • v.48 no.2
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    • pp.269-274
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    • 2009
  • Rearing media were selected for the production of sterile larvae of greenbottle blowfly, Phaenicia (=Lucilia) sericata (Meigen) which is widely used in maggot therapy. Eight media available in the market were used in this study. Egg hatchability was not different among the media. Survivorship of the larvae was higher in blood agar (BA), sabouraud dextrose agar, and brucella blood agar than the others. A higher content (20${\sim}$40%) of sheep blood in BA and chocolate agar increased the survivorship of larvae. The eggs and the early 3rd larvae could be stored for 12 days at $8^{\circ}C$ and for 15 days at $6^{\circ}C$ without decrease in hatchability and larval survivorship, respectively.

Effects of rearing environment on oviposition preference of black soldier fly (Hermetia illucens) (아메리카동애등에(Hermetia illucens)의 성충 사육환경이 산란 선호성에 미치는 영향)

  • Choi, Myung-Hyo;Yang, Young-Cheol;Kang, Seung-Ho;Park, Young-Kyu
    • Journal of Sericultural and Entomological Science
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    • v.54 no.1_2
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    • pp.17-22
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    • 2016
  • Rearing environment direct effects on oviposition preference of black soldier fly(Hermetia illucens). In this study, the number of egg-filled flutes was surveyed according to the size of adult rearing cages and oviposition media of black soldier fly (BSF). The height of the adult rearing cages were fixed at 2 m and provided different sizes of width and length and released 20,000 BSF adults in the rearing cages. The mean number of BSF egg-filled flutes was 895.4 at $4{\times}4m$ cage, 876.3 at $2{\times}1m$ cage and 62.3 at $1{\times}1m$ cage. Comparison of egg-filled flutes was according to cages material of adult BSF. The mean number of egg-filled flutes were 820.7 and 604.3 at different cage materials such as mosquito net and green house film. The mean numbers of egg-filled flutes were 326.6 in green floral form, 135.2 in green corrugated cardboard and 41.0 in brown corrugated cardboard. And the mean number of egg-filled flutes were similar floral form with the corrugated cardboard was overlapped by floral form such as 57.7 and 64.7. The mean number of egg-filled flutes was 29.0 and 48.2 in 6 mm and 4 mm diameter of oviposition hole.

Determining the doses of probiotics for application in Scylla tranquebarica (Fabricius 1798) larvae to produce crablet

  • Gunarto, Gunarto;Yustian Rovi Alfiansah;Muliani Muliani;Bunga Rante Tampangalo;Herlinah Herlinah;Nurbaya Nurbaya;Rosmiati Rosmiati
    • Fisheries and Aquatic Sciences
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    • v.27 no.3
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    • pp.180-194
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    • 2024
  • Mass mortalities of mud crab Scylla spp. larvae due to pathogenic Vibrio spp. outbreaks have frequently occurred in hatcheries. To overcome this problem, probiotics containing Bacillus subtilis bacteria are applied to inhibit pathogenic ones. We tested different doses of probiotic-containing B. subtilis (108 CFU/g) on the Scylla tranquebarica larvae and investigated the microbiota population, including Vibrio. Water quality, larvae development, and crablet production were also monitored. The recently hatched larvae were grown in twelve conical fiber tanks filled with 200 L sterile seawater, with a salinity of 30 ppt at a stocking density of 80 ind/L. Four different doses of probiotics were applied in the larvae rearing, namely, A = 2.5 mg/L, B = 5 mg/L, C = 7.5 mg/L, and D = 0 mg/L, with three replicates. Next-generation sequencing analysis was used to obtain the abundance of microbes in the whole body of megalopa and the water media for larvae rearing after applying probiotics. Sixteen Raw Deoxyribonucleic Acid samples (eight from a whole body of megalopa extraction from four treatments of probiotics defined as A, B, C, D, and eight from water media extraction from four treatments of probiotic defined as E, F, G, H) were prepared. Then, they were sent to the Genetics Science Laboratory for NGS analysis. Ammonia, nitrite, total organic matter (TOM), larvae, and crablet production were monitored. Based on the Next-generation sequencing analysis data, the Vibrio spp. decreased significantly (p < 0.05) than control test (D) in megalopa-applied probiotics at the doses of 2.5 mg/L (A) and 7.5 mg/L (C) and in the water media for megalopa rearing treated with probiotics at the dosage of 5.0 mg/L (F). Ammonia in the zoea stage in B treatment and TOM in the zoea and megalopa stage in B and C treatments were decreased significantly (p < 0.05). It impacts the higher number of zoea survival in treatments B and C. Finally, it results in a significantly high crablet production in treatments B and C. Therefore, the dosage of 5 mg/L to 7.5 mg/L improves crablet S. tranquebarica production significantly.

A Study for Growth Strategy of the 21C Printing Information Industry (21세기 인쇄 정보산업의 발전 전략에 관한 연구)

  • Oh, Sung-Sang
    • Journal of the Korean Graphic Arts Communication Society
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    • v.20 no.2
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    • pp.45-56
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    • 2002
  • In these day, our life has been developed with the computer. Printing Industry was introduced the information industry as computer, internet, etc. These are now in daily use and covers the entire work flow - from the planing of the product and the production at domestic printing industry. Then, I suggest the correspondence of changing printing work flow, understanding of digital printing technique, rearing of a special line of business, adjustment in the environment changing of printing technique etc. for the purpose of development of domestic printing industry.

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A Survey on the Experience of Accident of Infant, Toddler and Preschooler, and the Education about Emergency Care for Parents (영 유아 및 학령전 아동의 안전사고 및 어머니의 응급처치 정보 접촉 경험에 관한 조사 연구)

  • Kim Il-Ok;Shin Sun-Hwa
    • Child Health Nursing Research
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    • v.8 no.2
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    • pp.174-182
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    • 2002
  • The purpose of this study was to investigate the experiences of accident and visiting emergency center, and the experiences of education about emergency care for parents. The subjects of this study were 117 mothers whose children are attending 9 different nursery schools or kindergartens. The instrument used in this study was developed by researchers and validated by nursing professor, first aid doctor and the president of nursery school, and consisted of 'most important thing in child rearing' ,'experiences of accident and visiting emergency center', 'type of trauma' and 'need for parent education program' the result of this study were as followed ; 1.There were 70.09% of the experience of visiting emergency center among the subjects. The cause of visiting emergency center were high fever and convulsion(42.86%), and trauma by accident(28.04%) 2. The most common type of the indoor accident were fall down and slipping(76.92%), the common type of trauma were contusion (76.92%), abrasion(47.01%), laceration(29.06%), dislocation or fracture(12.82%), burn(10.26%), piercing(8.55%). 3.47.01% of subjects had the experience of education about emergency care for parents. They were educated by child rearing journals (32.72%), small books which were published by hospitals or community health center (25.45%), mass media(21.8%), parent seminar (12.72%), nursery school or kindergarten (5.45%). In conclusion, the need of emergency care for children was strongly found. Therefore, on the basis of above results, the parent education program which fits their cognitive level and their health care needs So, it must be directed on the further study of parents' knowledge about emergency care for children. To enhance the effectiveness of program and accomplish the children's health promotion, advanced instructional media and demonstration must be included.

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Survivorships of Gammarus sobaegensis (Amphipoda: Gammaridae) in Different Medium and Temperature Conditions (보통옆새우 (Gammarus sobaegensis : 옆새우과, 단각목)의 서로 다른 배양액 및 온도에 대한 생존율)

  • Hwang, Jeong-Mi;Lee, Sung-Jin;Cho, Ki-Jong;Kim, Yong-Gyun;Bae, Yeon-Jae
    • Korean Journal of Ecology and Environment
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    • v.39 no.3 s.117
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    • pp.413-417
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    • 2006
  • Gammarus sobaegensis, a common amphipod crustacean in Korean mountain streams, was experimented in the laboratory in terms of two environmental factors, medium and temperature conditions, which are essential in laboratory rearing. Individuals of G. sobaegensis were collected from a small spring outlet beside Gapyeong stream in Gyeonggi-do, Korea, in December 2003. Survivorships of G. sobaegensis were evaluated from the effect on rearing media (distilled water: DW, tap water: TW, chironomid rearing medium: CM, and stream water: SW) and temperatures (10, 15, 20, and $25^{\circ}C$). As a result, G. sobaegensis was higher in survivorships at TW followed by SW, CM, and DW and at $10^{\circ}C$ followed by 15, 20 and $25^{\circ}C$.

Development of mass rearing technique of Tyrophnn putrescentiae (Acari: Acaridae) found in house dust (집먼지에 서식하는 긴털가루진드기(진드기목: 가루진드기과)의 대량 사육방법 개발)

  • 이한일;이인용
    • Parasites, Hosts and Diseases
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    • v.35 no.3
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    • pp.149-154
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    • 1997
  • A storage mite, Tyrophcgus putrescentiae, is recently known to be widely distributed in Korea, being commonly found in house dust, and may, therefore, be allergenically important. The purpose of this study was to develop mass rearing techniques for supplying a large quantity of allergens. The laboratory mouse food powder gave the highest yield, showing 1,251.5-fold increase in number after 10 weeks, and the mixed powder of laboratory mouse food and yeast (1 : 1) also gave same level of the production (1.203 1-fold increase in week 10). Several different combinations of temperature and relative humidity conditions were compared, and the maximum propagation was obtained at $25^{\circ}C$ and 64% RH, showing 960-fold increase in number. When the same amount of culture media was used the size of the culture container did not significantly influence the quantitative yield of T. putrescentice mites.

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Mass-rearing Method of the Fungus Gnat, Bradysia difformis (Sciaridae, Diptera) in Laboratory (실내에서 작은뿌리파리(Bradysia difformis, Sciaridae, Diptera)의 대량사육시스템)

  • Jang, Hyun Ju;Yoon, Heon;Kwon, Hey Ri;Yu, Yong Man;Youn, Young Nam
    • Korean journal of applied entomology
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    • v.57 no.2
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    • pp.57-64
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    • 2018
  • The fungus gnat, Bradysia difformis, has been recognized as an important pest of greenhouse crops. There is a need for research on the control of the fungus gnat. However, it is difficult to obtain many generations of the fungus gnat for several kinds of research. Indoor propagation is a very useful method for obtaining enough individuals in cases where the need is for larvae in soil. This study was conducted to determine the optimum growing media and temperature conditions for rearing the fungus gnat in the laboratory. Under experimental temperature conditions, hatching, pupation, and eclosion rates were the highest at $20^{\circ}C$. The developmental period of the fungus gnat was shortened with higher temperatures. The greatest number of eggs was an average of 144 at $20^{\circ}C$. Using different types of larvae growth media, the highest hatching rates were 84.7 and 84.4% in water agar and potato disks, respectively. The larval period was the shortest, at 14.7 days, when grown on potato disks. The highest pupation and eclosion rates were 85.2 and 82.6% on potato disks, respectively. The highest number of eggs was an average of 125.6 on potato disks. Regarding the effects of different growth media on the eclosion rate of B. difformis, the highest eclosion rate was 88.4% on the soil mix, and was 50% on oatmeal, 25% on shredded potato. The results of four different inoculation levels of larvae on eclosion rate of B. difformis showed that the highest eclosion rate was 84.7% for 1,000 larvae. The eclosion rate was shortened with a higher number of larvae inoculated/cage. In the growth medium used, 3,000 eggs were better for the initial level of inoculation, showing a relatively high emergence rate and short developmental period. Mass rearing procedures were explained in detail.

New Types of Masculinity Represented in TV and Its Limitations : Focusing on Weekend Variety Programs (TV매체에 재현된 새로운 남성성(masculinity)과 그 한계 -주말 예능프로그램을 중심으로-)

  • Kim, Mira
    • The Journal of the Korea Contents Association
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    • v.14 no.1
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    • pp.88-96
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    • 2014
  • This study attempts, based on the premise that gender roles and identity is a social construct, to show how TV portrayal of the male has changed through the years with changes in time and society, from the traditional depiction of hegemonic masculinity and ideal manhood as supported by the patriarchal system. A narrative analysis was conducted on popular variety shows "Dad, Where Are We Going?" and "Superman Returns". The results showed that both TV shows created a new type of masculinity by centering the narrative on the traditionally female roles of child rearing and housekeeping, and recreating the traditional strict and authoritative father figure into a non-authoritative and emotionally expressive father. However, as 'child rearing' and 'housekeeping' is expressed as 'play', there are limitations in that the actual daily lives and hardship of women is excluded from the narrative.

Bioaccumulation of Pb and Cd in Blue Mussel (Mytilus edulis) and Oliver Flounder (Paralichthys olivaceus) Exposed to Rearing Media (노출실험을 통한 진주담치(Mytilus edulis) 넙치(Paralichthys olivaceus)의 Pb 및 Cd 축적에 관한 연구)

  • Cho, Yeong-Gil;Kim, Gi-Beum
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.10 no.1
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    • pp.21-28
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    • 2007
  • The bioaccumulation of Pb and Cd dissolved in seawater was assessed measuring the concentrations recorded within blue mussel (Mytilus edulis) and oliver flounder (Paralichthys olivaceus) after two weeks exposure period. The Pb and Cd concentration within the whole body of two testing organisms increased according to the exposure concentrations, and the such tendency was clear specially from the mussel. Maximum metal concentration reached $5,260({\pm}70)\;{\mu}g/g$ for Pb reared under 5.0 mg/L Pb, $1,040({\pm}40)\;{\mu}g/g$ for Cd reared under 1.0 mg/L Cd in the mussel, and indicated that the bioaccumulation of Pb and Cd was directly related to the rearing medium concentrations. Bioconcentration factors (BCF) reached very high values for Pb (maximum value: $12,100{\pm}1,400$) in the mussel reared under lowest Pb concentration (0.01 mg/L). The BCF value for Cd in the mussel were also far higher at exposure to low Cd concentration than high Cd concentration. At higher external concentrations, the BCF for Cd and Pb declined. This demonstrated the ability of two testing organisms to rapidly uptake heavy metals particularly when exposed to low external concentration. The mean Pb concentration was slightly higher in the gill of mussel than in the digestive gland, while Cd showed a higher level in the digestive gland than in the gill.

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