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http://dx.doi.org/10.5656/KSAE.2009.48.2.269

Selection of Rearing Media, Proper Temperature and Period for Storage of Sterile Lucilia sericata (Diptera: Calliphoridae) Eggs and Larvae  

Jang, Sin-Ae (Institute of Agriculture and Life Science / Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang Nat'l Univ.)
Yun, Ji-Eun (Institute of Agriculture and Life Science / Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang Nat'l Univ.)
Park, Chung-Gyoo (Institute of Agriculture and Life Science / Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang Nat'l Univ.)
Publication Information
Korean journal of applied entomology / v.48, no.2, 2009 , pp. 269-274 More about this Journal
Abstract
Rearing media were selected for the production of sterile larvae of greenbottle blowfly, Phaenicia (=Lucilia) sericata (Meigen) which is widely used in maggot therapy. Eight media available in the market were used in this study. Egg hatchability was not different among the media. Survivorship of the larvae was higher in blood agar (BA), sabouraud dextrose agar, and brucella blood agar than the others. A higher content (20${\sim}$40%) of sheep blood in BA and chocolate agar increased the survivorship of larvae. The eggs and the early 3rd larvae could be stored for 12 days at $8^{\circ}C$ and for 15 days at $6^{\circ}C$ without decrease in hatchability and larval survivorship, respectively.
Keywords
Maggot therapy; Phaenicia; Diabetic ulcer; MDT; Rearing media;
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