• Asian-Australasian Journal of Animal Sciences
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• v.26 no.11
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• pp.1545-1552
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• 2013

This study was conducted to investigate the effects of Trichostatin A (TSA) on cumulus expansion during mouse oocyte maturation. TSA treatment inhibited cumulus expansion and significantly reduced the cumulus expansion index (CEI) (p<0.05). To determine the underlying mechanism, the expression levels of several key factors that play crucial roles in cumulus expansion including components of extracellular matrix (ECM) (Has2, Ptgs2, Ptx3, and Tnfaip6) and Growth differentiation factor 9 (GDF9) were measured in control and TSA treated samples by real-time PCR. The effect of TSA on ERK phosphorylation (p-ERK1/2) in cumulus cells and GDF9 protein level in fully grown oocytes (FGOs) were detected by Western blotting. The expression levels of the ECM genes were significantly decreased (p<0.05) by TSA treatment while GDF9 expression did not response to TSA (p>0.05). TSA treatment blocked the activation of ERK1/2 (p<0.05) and had no significant effect on GDF9 protein expression (p>0.05). Collectively, these results suggested that TSA treatment altered ECM gene expression and blocked ERK1/2 activation to inhibit cumulus expansion in the mouse.

• BMB Reports
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• v.38 no.2
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• pp.151-160
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• 2005

By a cDNA array representing 2308 signal transduction related genes, we studied the expression profiles of HeLa cells stably transfected by Hepatitis C virus nonstructural protein 4B (HCV-NS4B). The alterations of the expression of four genes were confirmed by real-time quantitative RT-PCR; and the aldo-keto reductase family 1, member C1 (AKR1C1) enzyme activity was detected in HCV-NS4B transiently transfected HeLa cells and Huh-7, a human hepatoma cell line. Of the 2,308 genes we examined, 34 were up-regulated and 56 were down-regulated. These 90 genes involved oncogenes, tumor suppressors, cell receptors, complements, adhesions, transcription and translation, cytoskeletion and cellular stress. The expression profiling suggested that multiple regulatory pathways were affected by HCV-NS4B directly or indirectly. And since these genes are related to carcinogenesis, host defense system and cell homeostatic mechanism, we can conclude that HCV-NS4B could play some important roles in the pathogenesis mechanism of HCV.

• Journal of the Korea Computer Graphics Society
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• v.17 no.2
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• pp.11-16
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• 2011

In computer graphics, shadows play a very important role as a hint of inter-object distance as well as themselves in terms of realism. To represent shadows, some traditional methods such as shadow mapping and shadow volume have been frequently used for the purpose. However, the rendering results are not natural since they assume the point light. On the contrary, an area light can render soft-shadows, but its computation is too burdensome due to integral over the whole light source surface. Many alternatives have been introduced, back-projection of occluder onto the light source to get visibility of light or filtering of shadow boundary by calculating size of penumbra. But they also have problems of light bleeding or ringing effects because of low order approximation, or low performance. In this paper, we describe a method to improve those problems using shadow atlas.

• Journal of Applied Biological Chemistry
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• v.51 no.1
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• pp.1-6
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• 2008

Estrogens, a group of steroid compounds functioning as the primary female sex hormone, play an important role in the development and progression of breast cancer. In this study, using a novel annealing control primer-based GeneFishing PCR technology, five differentially expressed genes (DEGs), expressed using 10nM mitogenic estrogens, $17{\beta}$-estradiol (E2) and $16{\alpha}$-hydroxyestrone ($16{\alpha}$-OHE1), were selected from the estrogen receptor (ER)-positive MCF-7 human breast cancer cells. The DEGs, MRPL42, TUBA1B, SSBP1, KNCT2, and RUVBL1, were identified by comparison with the known genes via direct sequencing and sequence homology search in BLAST. Quantitative real-time PCR data showed that two DEGs, tubulin ${\alpha}1b$ and kinetochore associated 2, were greater than 2-fold upregulated by E2 or $16{\alpha}$-OHE1. Both genes could be new biomarkers for the treatment and prognosis of cancers, and further study may provide insights into the molecular mechanisms underlying development and progression of breast cancer.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.23 no.4
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• pp.767-779
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• 2013

A field of privacy protection lacks statistical information about the current status, compared to other fields. On top of that, since it has not been classified as a concrete separate field, the related survey is only conducted as a part of such concrete areas. Furthermore, this trend of being regarded as a part of fields such as informatization, information protection and law will continue in the near future. In this paper, a novel and practical way for collecting and storing a big amout of data from 110,000 privacy policies by data controller is proposed and the real analysis results is also shown. The proposed method can save time and cost compared with the traditional survey-based method while maintaining or even advancing the accuracy of results and speediness of process. The collected big personal data can be used to set up various kinds of statistical models and they will play an important role as a breakthrough of observing the present status of privacy information protection policy. The big data concept is incorporated into the privacy protection and we can observe the method and some results throughout the paper.

• The KIPS Transactions:PartA
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• v.10A no.6
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• pp.757-768
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• 2003

Recently, the renewable energy resources play an important role as an energy resources of future due to an environmental pollution and lack of resources and so on. The development and diffusion of information systems related to renewable energy resources have been driven actively abroad. However, at domestic an efficient history management for renewable energy resource data and web retrieve service are not provided. Therefore, in this paper, we propose a history data management system for the renewable energy resources, which stores and manages vast history information of renewable energy resource data systematically. This system collects renewable energy resource data in the outside observation system and stores them in the history database. The stored Information is retrieved through the history query process and can be provided in various forms - table, graph, chart and counter line, etc. - on the internet. Especially, the proposed system manages the history data in real-time so the latest information is always provided to the users through the web interface.

• The KIPS Transactions:PartC
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• v.16C no.4
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• pp.487-494
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• 2009

VOD(Video On Demand) that provides streaming service according to the user's requirement in real time, consists of the video streaming server and the client system. Since it is very hard to apply the traditional server-client model that a server communicates with many clients through 1:1 connection to VOD system because it requires very high network bandwidth, many researches have been done to address this problem. Batching technique is one of VOD system based on Multicast that requires very small network bandwidth. However, the batching based VOD system has a limitation that it is very hard to provide VCR(Video Cassette Recorder) ability. In this paper, we propose a technique that reduces the required network bandwidth to provide VCR function by using the characteristic of MPEG, one of international video compression standard. In the proposed technique, a new video stream for VCR function is constructed with I pictures that is able to be decoded independently. The new video stream for VCR function is transmitted with the video stream for normal play together in Batching manner. The performance evaluation result shows that the proposed technique not only reduces the required network bandwidth and memory usage but also decreases the CPU usages.

• Journal of KIISE:Computing Practices and Letters
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• v.12 no.5
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• pp.286-299
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• 2006

Due to the continuing technical progress in the capabilities of modeling, simulation, and sensor devices, huge volume data with very high resolution are common. In scientific visualization, various interactive real-time techniques on high performance parallel computers to effectively render such large scale volume data sets have been proposed. In this paper, we present a mobile volume visualization system that consists of mobile clients, gateways, and parallel rendering servers. The mobile clients allow to explore the regions of interests adaptively in higher resolution level as well as specify rendering / viewing parameters interactively which are sent to parallel rendering server. The gateways play a role in managing requests / responses between mobile clients and parallel rendering servers for stable services. The parallel rendering servers visualize the specified sub-volume with rendering contexts from clients and then transfer the high quality final images back. This proposed system lets multi-users with PDA simultaneously share commonly interesting parts of huge volume, rendering contexts, and final images through CSCW(Computer Supported Cooperative Work) mode.

• Journal of Embryo Transfer
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• v.31 no.4
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• pp.323-333
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• 2016

Pro-inflammatory cytokines, interleukin-$1{\beta}$(IL1B), IL6, and tumor necrosis factor-alpha (TNF), are known to play important roles in regulating the endometrial function in the uterus during the estrous cycle and pregnancy in several species. However, the expression and function of these cytokines and their receptors in the uterine endometrium during the estrous cycle have not been studied in pigs. Thus, this study determined the expression and regulation of IL1B, IL6, TNF and their respective receptors, IL1R1, IL1RAP, IL6R, GP130, TNFRSF1A, and TNFRSF1B during the estrous cycle in pigs. To analyze levels of each gene expression in the uterine endometrium we obtained from endometrial tissues on Days 0, 3, 6, 9, 12, 15, and 18 of the estrous cycle. Real-time RT-PCR analysis showed that levels of IL1B, IL1RAP, IL6R, GP130, TNF, TNFRSF1A, and TNFRSF1B mRNAs were highest on Day 15 or 18 of the estrous cycle, which corresponds to the proestrus period. Levels of IL1R1 were highest on Day 0, while levels of IL6 were biphasic with high levels on Day 6 and Day 15. The abundance of IL1B, IL6, IL6R, and TNF mRNAs was decreased by progesterone, while levels of GP130 were increased by progesterone in endometrial tissue explants. These results showed that expression of pro-inflammatory cytokines and their receptors changed stage-specifically during the estrous cycle and regulated by progesterone in the uterine endometrium in pigs, suggesting that these pro-inflammatory cytokines may be involved in the regulation endometrial function during the estrous cycle in pigs.

• The Korean Journal of Pain
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• v.31 no.3
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• pp.147-154
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• 2018

Fibromyalgia (FM) is a contested illness with ill-defined boundaries. There is no clearly defined cut-point that separates FM from non-FM. Diagnosis of FM has been faced with several challenges that occur, including patients' health care-seeking behavior, symptoms recognition, and FM labeling by physicians. This review focuses on important but less visible factors that have a profound influence on under- or over-diagnosis of FM. FM shows different phenotypes and disease expression in patients and even in one patient over time. Psychosocial and cultural factors seem to be a contemporary ferment in FM which play a major role in physician diagnosis even more than having severe symptom levels in FM patients. Although the FM criteria are the only current methods which can be used for classification of FM patients in surveys, research, and clinical settings, there are several key pieces missing in the fibromyalgia diagnostic puzzle, such as invalidation, psychosocial factors, and heterogeneous disease expression. Regarding the complex nature of FM, as well as the arbitrary and illusory constructs of the existing FM criteria, FM diagnosis frequently fails to provide a clinical diagnosis fit to reality. A physicians' judgment, obtained in real communicative environments with patients, beyond the existing constructional scores, seems the only reliable way for more valid diagnoses. It plays a pivotal role in the meaning and conceptualization of symptoms and psychosocial factors, making diagnoses and labeling of FM. It is better to see FM as a whole, not as a medical specialty or constructional scores.