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http://dx.doi.org/10.5713/ajas.2013.13128

Effects of Trichostatin A on Cumulus Expansion during Mouse Oocyte Maturation  

Du, Ming (Key Laboratory of Animal Genetics, Breeding and Reproduction,Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University)
Fu, Xiangwei (Key Laboratory of Animal Genetics, Breeding and Reproduction,Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University)
Zhou, Yanhua (Key Laboratory of Animal Genetics, Breeding and Reproduction,Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University)
Zhu, Shien (Key Laboratory of Animal Genetics, Breeding and Reproduction,Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.26, no.11, 2013 , pp. 1545-1552 More about this Journal
Abstract
This study was conducted to investigate the effects of Trichostatin A (TSA) on cumulus expansion during mouse oocyte maturation. TSA treatment inhibited cumulus expansion and significantly reduced the cumulus expansion index (CEI) (p<0.05). To determine the underlying mechanism, the expression levels of several key factors that play crucial roles in cumulus expansion including components of extracellular matrix (ECM) (Has2, Ptgs2, Ptx3, and Tnfaip6) and Growth differentiation factor 9 (GDF9) were measured in control and TSA treated samples by real-time PCR. The effect of TSA on ERK phosphorylation (p-ERK1/2) in cumulus cells and GDF9 protein level in fully grown oocytes (FGOs) were detected by Western blotting. The expression levels of the ECM genes were significantly decreased (p<0.05) by TSA treatment while GDF9 expression did not response to TSA (p>0.05). TSA treatment blocked the activation of ERK1/2 (p<0.05) and had no significant effect on GDF9 protein expression (p>0.05). Collectively, these results suggested that TSA treatment altered ECM gene expression and blocked ERK1/2 activation to inhibit cumulus expansion in the mouse.
Keywords
Mouse; Cumulus-oocyte Complex; Cumulus Expansion; TSA;
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1 Baker, A. H., D. R. Edwards, and G. Murphy. 2002. Metalloproteinase inhibitors: Biological actions and therapeutic opportunities. J. Cell Sci. 115:3719-3727.   DOI   ScienceOn
2 Barter, M. J., L. Pybus, G. J. Litherland, A. D. Rowan, I. M. Clark, D. R. Edwards, T. E. Cawston, and D. A. Young. 2010. Hdac-mediated control of erk- and pi3k-dependent tgf-beta-induced extracellular matrix-regulating genes. Matrix Biol. 29:602-612.   DOI   ScienceOn
3 Brush, M. H., A. Guardiola, J. H. Connor, T. P. Yao, and S. Shenolikar. 2004. Deactylase inhibitors disrupt cellular complexes containing protein phosphatases and deacetylases. J. Biol. Chem. 279:7685-7691.   DOI   ScienceOn
4 Buccione, R., B. C. Vanderhyden, P. J. Caron, and J. J. Eppig. 1990. Fsh-induced expansion of the mouse cumulus oophorus in vitro is dependent upon a specific factor(s) secreted by the oocyte. Dev. Biol. 138:16-25.   DOI   ScienceOn
5 Camaioni, A., V. C. Hascall, M. Yanagishita, and A. Salustri. 1993. Effects of exogenous hyaluronic acid and serum on matrix organization and stability in the mouse cumulus cell-oocyte complex. J. Biol. Chem. 268:20473-20481.
6 Chen, L., P. T. Russell, and W. J. Larsen. 1993. Functional significance of cumulus expansion in the mouse: Roles for the preovulatory synthesis of hyaluronic acid within the cumulus mass. Mol. Reprod. Dev. 34:87-93.   DOI   ScienceOn
7 Dong, J., D. F. Albertini, K. Nishimori, T. R. Kumar, N. Lu, and M. M. Matzuk. 1996. Growth differentiation factor-9 is required during early ovarian folliculogenesis. Nature 383:531-535.   DOI   ScienceOn
8 Dragovic, R. A., L. J. Ritter, S. J. Schulz, F. Amato, D. T. Armstrong, and R. B. Gilchrist. 2005. Role of oocyte-secreted growth differentiation factor 9 in the regulation of mouse cumulus expansion. Endocrinology 146:2798-2806.   DOI   ScienceOn
9 Dragovic, R. A., L. J. Ritter, S. J. Schulz, F. Amato, J. G. Thompson, D. T. Armstrong, and R. B. Gilchrist. 2007. Oocyte-secreted factor activation of smad 2/3 signaling enables initiation of mouse cumulus cell expansion. Biol. Reprod. 76:848-857.   DOI   ScienceOn
10 Elvin, J. A., A. T. Clark, P. Wang, N. M. Wolfman, and M. M. Matzuk. 1999. Paracrine actions of growth differentiation factor-9 in the mammalian ovary. Mol. Endocrinol. 13:1035-1048.   DOI   ScienceOn
11 Fagbohun, C. F. and S. M. Downs. 1990. Maturation of the mouse oocyte-cumulus cell complex: Stimulation by lectins. Biol. Reprod. 42:413-423.   DOI   ScienceOn
12 Lim, H., B. C. Paria, S. K. Das, J. E. Dinchuk, R. Langenbach, J. M. Trzaskos, and S. K. Dey. 1997. Multiple female reproductive failures in cyclooxygenase 2-deficient mice. Cell. 91:197-208.   DOI   ScienceOn
13 Fulop, C., S. Szanto, D. Mukhopadhyay, T. Bardos, R. V. Kamath, M. S. Rugg, A. J. Day, A. Salustri, V. C. Hascall, T. T. Glant, and K. Mikecz. 2003. Impaired cumulus mucification and female sterility in tumor necrosis factor-induced protein-6 deficient mice. Development 130:2253-2261.   DOI   ScienceOn
14 Gui, L. M. and I. M. Joyce. 2005. Rna interference evidence that growth differentiation factor-9 mediates oocyte regulation of cumulus expansion in mice. Biol. Reprod. 72:195-199.   DOI   ScienceOn
15 Hayashi, M., E. A. McGee, G. Min, C. Klein, U. M. Rose, M. van Duin, and A. J. Hsueh. 1999. Recombinant growth differentiation factor-9 (gdf-9) enhances growth and differentiation of cultured early ovarian follicles. Endocrinology 140:1236-1244.   DOI   ScienceOn
16 McPherron, A. C. and S. J. Lee. 1993. Gdf-3 and gdf-9: Two new members of the transforming growth factor-beta superfamily containing a novel pattern of cysteines. J. Biol. Chem. 268:3444-3449.
17 Mukhopadhyay, D., A. Asari, M. S. Rugg, A. J. Day, and C. Fulop. 2004. Specificity of the tumor necrosis factor-induced protein 6-mediated heavy chain transfer from inter-alpha-trypsin inhibitor to hyaluronan: Implications for the assembly of the cumulus extracellular matrix. J. Biol. Chem. 279:11119-11128.   DOI   ScienceOn
18 Mulholland, N. M., E. Soeth, and C. L. Smith. 2003. Inhibition of mmtv transcription by hdac inhibitors occurs independent of changes in chromatin remodeling and increased histone acetylation. Oncogene 22:4807-4818.   DOI   ScienceOn
19 Nair, A. R., L. J. Boersma, L. Schiltz, M. A. Chaudhry, and R. J. Muschel. 2001. Paradoxical effects of trichostatin a: Inhibition of nf-y-associated histone acetyltransferase activity, phosphorylation of hgcn5 and downregulation of cyclin a and b1 mrna. Cancer Lett. 166:55-64.   DOI   ScienceOn
20 Ochsner, S. A., A. J. Day, M. S. Rugg, R. M. Breyer, R. H. Gomer, and J. S. Richards. 2003. Disrupted function of tumor necrosis factor-alpha-stimulated gene 6 blocks cumulus cell-oocyte complex expansion. Endocrinology 144:4376-4384.   DOI   ScienceOn
21 Pujuguet, P., D. Radisky, D. Levy, C. Lacza, and M. J. Bissell. 2001. Trichostatin a inhibits beta-casein expression in mammary epithelial cells. J. Cell Biochem. 83:660-670.   DOI   ScienceOn
22 Salustri, A., C. Garlanda, E. Hirsch, M. De Acetis, A. Maccagno, B. Bottazzi, A. Doni, A. Bastone, G. Mantovani, P. P. Beck, G. Salvatori, D. J. Mahoney, A. J. Day, G. Siracusa, L. Romani, and A. Mantovani. 2004. Ptx3 plays a key role in the organization of the cumulus oophorus extracellular matrix and in in vivo fertilization. Development 131:1577-1586.   DOI   ScienceOn
23 Saunders, N., A. Dicker, C. Popa, S. Jones, and A. Dahler. 1999. Histone deacetylase inhibitors as potential anti-skin cancer agents. Cancer Res. 59:399-404.
24 Su, Y. Q., J. M. Denegre, K. Wigglesworth, F. L. Pendola, M. J. O'Brien, and J. J. Eppig. 2003. Oocyte-dependent activation of mitogen-activated protein kinase (erk1/2) in cumulus cells is required for the maturation of the mouse oocyte-cumulus cell complex. Dev. Biol. 263:126-138.   DOI   ScienceOn
25 Su, Y. Q., S. Rubinstein, A. Luria, Y. Lax, and H. Breitbart. 2001. Involvement of mek-mitogen-activated protein kinase pathway in follicle-stimulating hormone-induced but not spontaneous meiotic resumption of mouse oocytes. Biol. Reprod. 65:358-365.   DOI   ScienceOn
26 Su, Y. Q., K. Sugiura, K. Wigglesworth, M. J. O'Brien, J. P. Affourtit, S. A. Pangas, M. M. Matzuk, and J. J. Eppig. 2008. Oocyte regulation of metabolic cooperativity between mouse cumulus cells and oocytes: Bmp15 and gdf9 control cholesterol biosynthesis in cumulus cells. Development 135:111-121.
27 Su, Y. Q., K. Sugiura, Y. Woo, K. Wigglesworth, S. Kamdar, J. Affourtit, and J. J. Eppig. 2007. Selective degradation of transcripts during meiotic maturation of mouse oocytes. Dev. Biol. 302:104-117.   DOI   ScienceOn
28 Tsuji, N., M. Kobayashi, K. Nagashima, Y. Wakisaka, and K. Koizumi. 1976. A new antifungal antibiotic, trichostatin. J. Antibiot (Tokyo). 29:1-6.   DOI
29 Su, Y. Q., K. Wigglesworth, F. L. Pendola, M. J. O'Brien, and J. J. Eppig. 2002. Mitogen-activated protein kinase activity in cumulus cells is essential for gonadotropin-induced oocyte meiotic resumption and cumulus expansion in the mouse. Endocrinology 143:2221-2232.   DOI   ScienceOn
30 Suo, L., Q. G. Meng, Y. Pei, C. L. Yan, X. W. Fu, T. D. Bunch, and S. E. Zhu. 2010. Changes in acetylation on lysine 12 of histone h4 (ach4k12) of murine oocytes during maternal aging may affect fertilization and subsequent embryo development. Fertil. Steril. 93:945-951.   DOI   ScienceOn
31 Vanderhyden, B. C., P. J. Caron, R. Buccione, and J. J. Eppig. 1990. Developmental pattern of the secretion of cumulus expansion-enabling factor by mouse oocytes and the role of oocytes in promoting granulosa cell differentiation. Dev. Biol. 140:307-317.   DOI   ScienceOn
32 Vanderhyden, B. C., E. E. Telfer, and J. J. Eppig. 1992. Mouse oocytes promote proliferation of granulosa cells from preantral and antral follicles in vitro. Biol. Reprod. 46:1196-1204.   DOI   ScienceOn
33 Varani, S., J. A. Elvin, C. Yan, J. DeMayo, F. J. DeMayo, H. F. Horton, M. C. Byrne, and M. M. Matzuk. 2002. Knockout of pentraxin 3, a downstream target of growth differentiation factor-9, causes female subfertility. Mol. Endocrinol. 16:1154-1167.   DOI   ScienceOn
34 Xu, W. S., R. B. Parmigiani, and P. A. Marks. 2007. Histone deacetylase inhibitors: Molecular mechanisms of action. Oncogene 26:5541-5552.   DOI   ScienceOn