• Korean Journal of Microbiology
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• v.44 no.3
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• pp.169-177
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• 2008

Global gene expression of Deinococcus radiodurans, a highly radiation resistant bacterium, in response to excess copper was analyzed by using oligonucleotide microarray chip. Among 3,187 open reading frames of D. radiodurans, seventy genes showed a statistically significant expression ratio of at least 2-fold changes under growth conditions of excess copper; 64 genes were induced and 6 genes were reduced. Especially, two operons ($DRB0014{\sim}DRB0017$ and $DRB0125{\sim}DRB0121$) presumably involved in the iron transport and utilization were the most highly induced genes by excess copper. A quantitative real-time PCR assay revealed that DRB00l4 and DRB0125 are highly transcribed responding to excess copper and 2,2'-dipyridyl, an iron chelator. In addition, the transcription of both genes was not changed by excess iron and bathocuproine disulphonate, a copper chelator. These results suggested that the copper metabolism may be closely connected with the iron transport and utilization in D. radiodurans. However, the disruption of each gene, DRB00l4 and DRB0125, did not affect the copper and radiation resistance, the most well-known character of this organism.

• Journal of the Korea institute for structural maintenance and inspection
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• v.17 no.2
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• pp.1-9
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• 2013

In recent years, numerous mega-size and complex civil infrastructures have been constructed worldwide. For the more precise construction and maintenance process management of these civil infrastructures, the application of a variety of smart sensor-based structural health monitoring (SHM) systems is required. The efficient management of both sensors and collected databases is also very important. Recently, several kinds of database access technologies using Quick Response (QR) code and Augmented Reality (AR) applications have been developed. These technologies provide software tools incorporated with mobile devices, such as smart phone, tablet PC and smart pad systems, so that databases can be accessed very quickly and easily. In this paper, an AR-based structural health monitoring technique is suggested for sensor management and the efficient access of databases collected from sensor networks that are distributed at target structures. The global positioning system (GPS) in mobile devices simultaneously recognizes the user location and sensor location, and calculates the distance between the two locations. In addition, the processed health monitoring results are sent from a main server to the user's mobile device, via the RSS (really simple syndication) feed format. It can be confirmed that the AR-based structural health monitoring technique is very useful for the real-time construction process management of numerous mega-size and complex civil infrastructures.

• Journal of the Ergonomics Society of Korea
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• v.32 no.6
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• pp.481-487
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• 2013

Objective: The present study aims to evaluate the visual reactions of users when they are playing games of different flow levels, and to explore the visual variables that can sensitively reflect the different flow levels. Background: The flow is defined as a psychological state where interface users feel their actions in a virtual setting identical to those in real environment. To measure the flow states of users, the questionnaire-based FSS(Flow State Scale) has mostly been used. However, this method is a qualitative test that has limits in terms of the accuracy of users' flow experiences. Therefore, more accurate methods to measure users' flow experiences are required. Method: Ten subjects participated in the experiment, where the independent variables were three games with different flow levels(puzzle games, dot drawing and coloring) and the time frame(the first and last 10 seconds in game playing), whereas the dependent variables included the pupil size and the frequency and duration of eye blinking. This study was a within-subject design. Each participant performed three types of games with different flow levels 3 times for each for 10 minutes, and their visual reactions to each game were measured. Results: The higher the flow cause the bigger pupil size(p<0.01) and the lower eye blinking frequency(p<0.1), indicating that different types of games lead to different flow levels. The pupil size during the last 10 seconds when the flow level was higher was bigger by 2.1% compared with that during the first 10 seconds in game playing(p<0.1), and the eye blinking frequency decreased by 12%(p<0.01). Conclusion: It was found out that the pupil size and the frequency and duration of eye blinking were psychophysiological indices for evaluating users' flow experiences, which could quantify the flow states users go through. The psychophysiological variables capable of measuring diverse aspects of the flow need diversifying to be applicable to precise measurement of the flow. Application: These studies are warranted for both quantitative analysis of flow levels and qualitative improvement of cyber leisure in line with development of healthy games.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.49 no.2
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• pp.35-44
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• 2012

Most recent infrared (IR) sensors have a focal-plane array (FPA) structure. Spatial non-uniformity of a FPA structure, however, introduces unwanted fixed pattern noise (FPN) to images. This non-uniformity correction (NUC) of a FPA can be categorized into target-based and scene-based approaches. In a target-based approach, FPN can be separated by using a uniform target such as a black body. Since the detector response randomly drifts along the time axis, however, several scene-based algorithms on the basis of a video sequence have been proposed. Among those algorithms, the state-of-the-art one based on Kalman filter uses one-directional warping for motion compensation and only compensates for offset non-uniformity of IR camera detectors. The system model using one-directional warping cannot correct the boundary region where a new scene is being introduced in the next video frame. Furthermore, offset-only correction approaches may not completely remove the FPN in images if it is considerably affected by gain non-uniformity. Therefore, for FPN reduction in IR videos, we propose a joint correction algorithm of gain and offset based on bi-directional warping. Experiment results using simulated and real IR videos show that the proposed scheme can provide better performance compared with the state-of-the art in FPN reduction.

• Carbon letters
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• v.23
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• pp.38-47
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• 2017

In this study, magnetite ($Fe_3O_4$) nanoparticles were electrochemically synthesized in an aqueous electrolyte at a given potential of -1.3 V for 180 s. Scanning electron microscopy revealed that dendrite-like $Fe_3O_4$ nanoparticles with a mean size of < 80 nm were electrodeposited on a glassy carbon electrode (GCE). The $Fe_3O_4/GCE$ was utilized for sensing chloramphenicol (CAP) by cyclic voltammetry and square wave voltammetry. A reduction peak of CAP at the $Fe_3O_4/GCE$ was observed at 0.62 V, whereas the uncoated GCE exhibited a very small response compared to that of the $Fe_3O_4/GCE$. The electrocatalytic ability of $Fe_3O_4$ was mainly attributed to the formation of Fe(VI) during the anodic scan, and its reduction to Fe(III) on the cathodic scan facilitated the sensing of CAP. The effects of pH and scan rate were measured to determine the optimum conditions at which the $Fe_3O_4/GCE$ exhibited the highest sensitivity with a lower detection limit. The reduction current for CAP was proportional to its concentration under optimized conditions in a range of $0.09-47{\mu}M$ with a correlation coefficient of 0.9919 and a limit of detection of $0.09{\mu}M$ (S/N=3). Moreover, the fabricated sensor exhibited anti-interference ability towards 4-nitrophenol, thiamphenicol, and 4-nitrobenzamide. The developed electrochemical sensor is a cost effective, reliable, and straightforward approach for the electrochemical determination of CAP in real time applications.

• Journal of the Korea Society of Computer and Information
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• v.17 no.6
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• pp.163-172
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• 2012

Collaborative filtering which is used explicit method in a existing recommedation system, can not only reflect exact attributes of item but also still has the problem of sparsity and scalability, though it has been practically used to improve these defects. This paper proposes the personalized recommendation system using RFM method and k-means clustering in u-commerce which is required by real time accessablity and agility. In this paper, using a implicit method which is is not used complicated query processing of the request and the response for rating, it is necessary for us to keep the analysis of RFM method and k-means clustering to be able to reflect attributes of the item in order to find the items with high purchasablity. The proposed makes the task of clustering to apply the variable of featured vector for the customer's information and calculating of the preference by each item category based on purchase history data, is able to recommend the items with efficiency. To estimate the performance, the proposed system is compared with existing system. As a result, it can be improved and evaluated according to the criteria of logicality through the experiment with dataset, collected in a cosmetic internet shopping mall.

• The Korean Journal of Physiology and Pharmacology
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• v.11 no.1
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• pp.1-7
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• 2007

Altered environmental gravity, including both hypo- and hypergravity, may result in space adaptation syndrome. To explore the characteristics of this adaptive plasticity, the expression of immediate early gene c-fos mRNA in the vestibular related tissues following an exposure to hypergravity stimulus was determined in rats. The animals were subjected to a force of 2 g (twice earth's gravity) for 1, 3, or 12 h, and were examined poststimulus at 0, 2, 6, 12, and 24 h. RT-PCR (reverse transcription polymerase chain reaction) and real-time quantitative RT-PCR were adopted to analyze temporal changes in the expression of c-fos mRNA. The hypergravity stimulus increased the expression of c-fos mRNA in the vestibular ganglion, medial vestibular nucleus, inferior vestibular nucleus, hippocampus, cerebellum, and cortex. The peak expression occurred at 0 h poststimulation in animals stimulated with hypergravity for 1 h, and at 6 h poststimulus in those stimulated for 3 h. In contrast, those stimulated for 12 h exhibited dual peaks at 0 and 12 h poststimulus. Bilateral labyrinthectomy markedly attenuated the degree of c-fos mRNA expression. Glutamate receptor antagonist also dramatically attenuated the degree of c-fos mRNA expression. These results indicate that expression of c-fos mRNA in response to hypergravity occurs in the vestibular related tissues of the central nervous system, in which peripheral vestibular receptors and glutamate receptors play an important role. The temporal pattern of c-fos mRNA expression depended on the duration of the hypergravity stimulus.

• Journal of the Korean Institute of Gas
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• v.12 no.2
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• pp.38-41
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• 2008

In this research, we propose a new method for tracking the release rate using the concentration data obtained from the sensor. We used a sensor network that has already been set surrounding the area where hazardous gas releases can occur. From the real-time sensor data, we detected and analyzed releases of harmful materials and their concentrations. Based on the results, the release rate is estimated using the neural network. This model consists of 14 input variables (sensor data, material properties, process information, meteorological conditions) and one output (release rate). The dispersion model then performs the simulation of the expected dispersion consequence by combining the sensor data, GIS data and the diagnostic result of the source term. The result of this study will improve the safety-concerns of residents living next to storage facilities containing hazardous materials by providing the enhanced emergency response plan and monitoring system for toxic gas releases.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.9
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• pp.314-321
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• 2016

After the 1980s, as the economy was growing rapidly and the real estate market was active, a large increase in population was supplied to the city. Accordingly, the national apartment housing market is located in the form of Urban Housing Culture. On the other hand, with time, the supply apartment houses became superannuated and the residential environment became poor. Therefore, the environment of old apartment houses has been changed to improve by reconstruction. Despite this, reconstruction incurred not only a lot of construction waste, but also problems of environmental degradation, noise, traffic congestion, increasing number of households, and so on. Recently, the housing law has changed, and vertical extension remodeling focused on Small/Medium Town House is underway. This has been changed in response to the demand. This is one of essential parts in the remodeling field for the future, even though it has some problems in how uses existing buildings. Therefore, this study analyzed occurrence risk factor for each type through research materials and case analysis regarding the vertical extension remodeling of apartment housing. In addition, the relative importance was determined through questionnaires and interviews from constructors, designers, and experts in the field.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.9
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• pp.292-301
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• 2016

Because damages arising from the occurrence of foot-and-mouth disease (FMD) are very great, it is essential to make a preemptive diagnosis to cope with it in order to minimize those damages. The main symptoms of foot-and-mouth disease are body temperature increase, loss of appetite, formation of blisters in the mouth, on hooves and breasts, etc. in a cow or a bull, among which the body temperature check is the easiest and quickest way to detect the disease. In this paper, an algorithm to detect FMD from the hooves of cattle was developed and implemented for preemptive coping with foot-and-mouth disease, and a hoof check test is conducted after the installation of a high-resolution camera module, a thermo-graphic camera, and a temperature/humidity module in the cattle shed. Through the algorithm and system developed in this study, it is possible to cope with an early-stage situation in which cattle are suspected as suffering from foot-and-mouth disease, creating an optimized growth environment for cattle. In particular, in this study, the system to cope with FMD does not use a portable thermo-graphic camera, but a fixed camera attached to the cattle shed. It does not need additional personnel, has a function to measure the temperature of cattle hooves automatically through an image algorithm, and includes an automated alarm for a smart phone. This system enables the prediction of a possible occurrence of foot-and-mouth disease on a real-time basis, and also enables initial-stage disinfection to be performed to cope with the disease without needing extra personnel.