• Journal of Microbiology and Biotechnology
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• v.15 no.6
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• pp.1353-1358
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• 2005

A rapid and quantitative real-time PCR was developed to target the invasion A (invA) gene of Salmonella spp. We developed quantitative standard curves based on plasmids containing the invA gene. Based on these curves, we detected Salmonella spp. in artificially contaminated buffered peptone water (BPW) and milk samples. We were able to determine the invA gene copy number per ml of food samples, with the minimum detection limit of $4.1{\times}10^{3}$ copies/ml of BPW and $3.3{\times}10^{3}$ copies/ml of milk. When applied directly to detect and quantify Salmonella spp. in BPW and milk, the present real-time PCR assay was as sensitive as the plate count method; however, copy numbers were one to two logs higher than the colony-forming units obtained by the plate count methods. In the present work, the real-time PCR assay was shown to significantly reduce the total time necessary for the detection of Salmonella spp. in foods and to provide an important model for other foodborne pathogens.

• Structural Engineering and Mechanics
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• v.62 no.5
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• pp.631-642
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• 2017

Accurate actuator tracking plays an important role in real-time hybrid simulation (RTHS) to ensure accurate and reliable experimental results. Frequency-domain evaluation index (FEI) interprets actuator tracking into amplitude and phase errors thus providing a promising tool for quantitative assessment of real-time hybrid simulation results. Previous applications of FEI successfully evaluated actuator tracking over the entire duration of the tests. In this study, FEI with moving window technique is explored to provide post-experiment localized actuator tracking assessment. Both moving window with and without overlap are investigated through computational simulations. The challenge is discussed for Fourier Transform to satisfy both time domain and frequency resolution for selected length of moving window. The required data window length for accuracy is shown to depend on the natural frequency and structural nonlinearity as well as the ground motion input for both moving windows with and without overlap. Moving window without overlap shows better computational efficiency and has potential for future online evaluation. Moving window with overlap however requires much more computational efforts and is more suitable for post-experiment evaluation. Existing RTHS data from Network Earthquake Engineering Simulation (NEES) is utilized to further demonstrate the effectiveness of the proposed approaches. It is demonstrated that with proper window size, FEI with moving window techniques enable accurate localized evaluation of actuator tracking for real-time hybrid simulation.

• Journal of information and communication convergence engineering
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• v.9 no.2
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• pp.244-250
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• 2011

Gossip is a well-known protocol which was proposed to implement broadcast service with a high reliability in an arbitrarily connected network of sensor nodes. The probabilistic techniques employed in gossip have been used to address many challenges which are caused by flooding in wireless sensor networks (WSNs). However, very little work has yet been done on real-time wireless sensor networks which require not only highly reliable packets reception but also strict time constraint of each packet. Moreover, the unique energy constraining feature of sensor makes existing solutions unsuitable. Combined with unreliable links, redundant messages overhead in real-time wireless sensor networks is a new challenging issue. In this paper, we introduce a Reliable Gossip Zone, a novel fine-tailored mechanism for real-time wireless sensor networks with unreliable wireless links and low packet redundancy. The key idea is the proposed forwarding probability algorithm, which makes forwarding decisions after the realtime flooding zone is set. Evaluation shows that as an oracle broadcast service design, our mechanism achieves significantly less message overhead than traditional flooding and gossip protocols.

• Journal of KIISE:Computing Practices and Letters
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• v.16 no.4
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• pp.437-441
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• 2010

A new application area in which wireless sensor networks are applied requires the performance of more elaborated and complicated task and the completion of those tasks within a time limit. Until now, it is, however, insufficient to do research on the mechanism of handling interrupt based on real-time sensor operating systems which carefully consider the limitation of resources of sensor nodes and the property of tasks which is executed in a wireless sensor network area. In this paper, the requirements satisfying real-time in sensor operating systems are analyzed and based on this, a system is designed and implemented. In addition, the proposed mechanisms are confirmed by several verification methods, and the efficiency of the performance and the satisfaction of those requirements for real-time are verified by simulation.

• 한국정보디스플레이학회:학술대회논문집
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• 2008.10a
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• pp.51-54
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• 2008

In this paper, we propose a practical method for displaying 2D/True3D mixed contents in real-time. Many companies released their 3D display recently, but the costs of producing True3D contents are still very expensive. Since there are already a lot of 2D contents in the world and it is more effective to mix True3D objects into the 2D contents than making True3D contents directly, people became interested in mixing 2D/True3D contents. Moreover, real-time 2D/True3D mixing is helpful for 3D displays because the scenario of the contents can be easily changed on playback-time by adjusting the 3D effects and the motion of the True3D object interactively. In our system, True3D objects are rendered into multiple view-point images, which are composed with 2D contents by using depth information, and then they are multiplexed with pre-generated view masks. All the processes are performed on a graphics processor. We were still able to play a 2D/True3D mixed contents with Full HD resolution in real-time using a normal graphics processor.

• Journal of Drive and Control
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• v.16 no.4
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• pp.38-47
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• 2019

In this paper, a real-time simulation model of an excavator using Amesim was proposed, considered the operation of functional valves with the main control valve (MCV). The hydraulic system models including the pump and MCV have been developed. The kinematic and dynamic models of the manipulator have also been developed, to confirm the behavior of the excavator. The MCV model includes various functional valves such as the regenerative valves, holding valves, swing and boom priority valves, and regen-cut valves so that simulations similar to real excavators can be performed. Additionally, to obtain the real-time calculation performance, the parts with no major influence on the dynamic behavior were simplified, high frequency factors were removed, and parameters were optimized. The models were compared with each other through the numerical analysis with variable time-step and fixed time-step, and the results were verified by comparison with the results of the actual vehicle tests.

• Journal of Hydrogen and New Energy
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• v.26 no.2
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• pp.184-191
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• 2015

As the power electronics system increases the frequency, the power loss and thermal management are paid more attention. This research presents a real time model of dissipation power with junction temperature response for 120kw IGBT inverter which is applied to the thermal management of high power IGBT inverter. Since the computational time is critical for real time simulation, look-up tables of IGBT module characteristic curve are implemented. The power loss from IGBT provides a clue to calculate the temperature of each module of IGBT. In this study, temperature of each layer in IGBT is predicted by lumped capacitance analysis of layers with convective heat transfer. The power loss and temperature of layers in IGBT is then communicated due to mutual dependence. In the dynamic model, PWM pulses are employed to calculation real time IGBT and diode power loss. Under Matlab/Simulink$^{(R)}$ environment, the dynamic model is validated with experiment. Results showed that the dynamic response of power loss is closely coupled with effective thermal management. The convective heat transfer is enough to achieve proper thermal management under guideline temperature.

• Speech Sciences
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• v.6
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• pp.219-233
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• 1999

This paper presents a real-time implementation of a speaker independent speech recognition system based on a discrete hidden markov model(DHMM). This system is developed for a car navigation system to design on-chip VLSI system of speech recognition which is used by fixed point Oak DSP core of DSP GROUP LTD. We analyze recognition procedure with C language to implement fixed point real-time algorithms. Based on the analyses, we improve the algorithms which are possible to operate in real-time, and can verify the recognition result at the same time as speech ends, by processing all recognition routines within a frame. A car noise is the colored noise concentrated heavily on the low frequency segment under 400 Hz. For the noise robust processing, the high pass filtering and the liftering on the distance measure of feature vectors are applied to the recognition system. Recognition experiments on the twelve isolated command words were performed. The recognition rates of the baseline recognizer were 98.68% in a stopping situation and 80.7% in a running situation. Using the noise processing methods, the recognition rates were enhanced to 89.04% in a running situation.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.25 no.3
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• pp.486-494
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• 2001

This paper presents a real-time multibody vehicle dynamic analysis method using recursive Kanes formulation and suspension composite joints. To shorten the computation time of simulation, relative coordinate system is used and the equations of motion are derived using recursive Kanes formulation. Typical suspension systems of vehicles such as MacPherson strut suspension system is modeled by suspension composite joints. The joints are derived and utilized to reduce the computation time of simulation without any degradation of kinematical accuracy of the suspension systems. Using the develop program, a multibody vehicle dynamic model is formed and simulations are performed. Accuracy of the simulation results is compared to the real vehicle field test results. It is found that the simulation results using the proposed method are very accurate and real-time simulation is achieved on a computer with single PowerPC 604 processor.

• Journal of Institute of Control, Robotics and Systems
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• v.22 no.7
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• pp.570-577
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• 2016

This paper proposes the design of a novel distributed message management scheme using a message-oriented management and analysis tool (MOMAT) for naval combat system (NCS) middle-ware. If a message is not guaranteed real-time of the NCS with each node, it causes the loss of data and decreases the reliability of systems. To solve these problems, improved message management schemes are proposed. Message management schemes are considering a real-time user management scheme and a real-time traffic management scheme. The proposed schemes are simulated with a developed simulation tool, data publisher, and subscriber connected through nodes in middle-ware. The simulation results show improved results in terms of message round-trip time (RTT), End-to-End delay, and throughput.