• Microbiology and Biotechnology Letters
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• v.36 no.3
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• pp.173-181
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• 2008

Bovine blood, cell, tissue, and organ are used as raw materials for manufacturing biologics such as biopharmaceuticals, tissue-engineered products, and cell therapy. Manufacturing processes for the biologics have the risk of viral contamination. Therefore viral validation is essential in ensuring the safety of the products. Bovine parvovirus (BPV) is one of the common bovine pathogens and has widely been known as a possible contaminant of biologics. In order to establish the validation system for the BPV safety of biologics, a real-time PCR method was developed for quantitative detection of BPV contamination in raw materials, manufacturing processes, and final products. Specific primers for amplification of BPV DNA were selected, and BPV DNA was quantified by use of SYBR Green 1. The sensitivity of the assay was calculated to be $1.3{\times}10^{-1}\;TCID_{50}/mL$. The real-time PCR method was validated to be reproducible and very specific to BPV. The established real-time PCR assay was successfully applied to the validation of Chinese hamster ovary (CHO) cell artificially infected with BPV. BPV DNA could be quantified in CHO cell as well as culture supernatant. Also the real-time PCR assay could detect $1.3{\times}10^0\;TCID_{50}/mL$ of BPV artificially contaminated in bovine collagen. The overall results indicated that this rapid, specific, sensitive, and robust assay can be reliably used for quantitative detection of BPV contamination during manufacture of biologics.

• Korean Journal of Food Science and Technology
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• v.43 no.1
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• pp.119-123
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• 2011

In this study, performances of culture methods using two selective media and real-time PCR were evaluated for detection of Campylobacter jejuni (C. jejuni) in various food samples. Sausage, ground beef, and radish sprouts inoculated with C. jejuni were enriched in Hunt broth and then streaked onto modified cefoperazone charcoal deoxycholate agar and Preston agar, followed by incubation under microaerobic conditions. The enriched Hunt broth (1 mL) was used in real-time PCR assay. No statistical differences were observed in sensitivity among the two selective media and real-time PCR for sausage and ground beef. However, the number of positives by real-time PCR in radish sprouts was much higher than the two selective media (p<0.05). It appears that real-time PCR could be used as an effective screening tool to detect C. jejuni, particularly in foods with a high number of background microflora such as fresh vegetables.

• Korean Journal of Microbiology
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• v.41 no.3
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• pp.168-176
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• 2005

Vibrio vulnificus, one of the marine bacterial pathogens causing septicemia, was detected using molecular methods, namely, PCR and/or Southern hybridization, and real-time PCR. Extracted and purified total DNAs by using commercial kits were used as templates for PCR. Multiplex-PCR was conducted by employing three sets of primers for the genes, hemolysin (vvhA), phosphomannomutase (pmm), and metalloprotease (vvpE), for V vulnificus virulence. The presence of DMSO ($5\%$) and BSA ($0.1\%$) in PCR reaction mixture improved a detection efficiency by higher PCR band intensities. TaqMan real-time PCR was carried out by using gene segment of vvhA as a target. Detection limit of PCR/Southern hybridization without enrichments was to be around $10^2\;cells\;g^{-1}$ of sample. However, those three methods using the enrichment at $35^{\circ}C$ in APW showed high sensitivity ($2\~10\;cells\;g^{-1}$ of sediments). Highly sensitive detection of V vulnificus by real-time PCR was achieved within $5\~6$ hr, whereas the detection by PCR/Southern hybridization required about 36 hr. Thus, it was evident that real-time PCR is the most rapid and efficient method for detecting V vulnificus in tidal flat sediments.

• Journal of Internet Computing and Services
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• v.19 no.4
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• pp.1-6
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• 2018

In smart cities, data from various kinds of sensors are collected and processed to provide smart services to the citizens. Noise information services with noise maps using the collected sensor data from various kinds of ubiquitous sensor networks is one of them. This paper presents a research result which generates three dimensional (3D) noise maps in real-time for smart cities. To make a noise map, we have to converge many informal data which include big image data of geographical Information and massive sensor data. Making such a 3D noise map in real-time requires the processing of the stream data from the ubiquitous sensor networks in real-time and the convergence operation in real-time. They are very challenging works. We developed our own methodology for real-time distributed and parallel processing for it and present it in this paper. Further, we developed our own real-time 3D noise map generation system, with the methodology. The system uses open source softwares for it. Here in this paper, we do introduce one of our systems which uses Apache Storm. We did performance evaluation using the developed system. Cloud computing was used for the performance evaluation experiments. It was confirmed that our system was working properly with good performance and the system can produce the 3D noise maps in real-time. The performance evaluation results are given in this paper, as well.

• Journal of Food Hygiene and Safety
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• v.36 no.4
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• pp.289-297
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• 2021

In this study, multiplex PCR and real-time PCR were performed on Theragra chalcogramma (walleye pollock), Pangasianodon hypophthalmus (iridescent shark) and their processed foods, such as changnan-jeot and gaiyang-jeot (salted iridescent shark intestine). Species-specific primers for T. chalcogramma and P. hypophthalmus were designed, and genomic DNA was directly extracted from each sample to perform single PCR and multiplex PCR. As a result of PCR, in the case of single PCR, PCR bands of T. chalcogramma (297 bp) and P. hypophthalmus (132 bp) were identified, and in the case of multiplex PCR, it was confirmed that amplification occurred without cross-reaction between T. chalcogramma and P. hypophthalmus. As a result of checking the PCR sensitivity, the concentration of genomic DNA was detected up to 0.1 ng/µL in both single PCR and multiplex PCR. The real-time PCR results showed that the average Ct value of T. chalcogramma was 20.765±0.691, and the average Ct value of P. hypophthalmus sample was 35.719±1.828 in the T. chalcogramma species-specific primers. In the P. hypophthalmus species-specific primers, the average Ct value of the T. chalcogramma sample was 35.996±1.423, and the mean Ct value of the P. hypophthalmus sample was 20.096±0.793. These results demonstrated the significant differences in the efficiency, specificity and cross-reactivity of species-specific primers in real-time PCR. Based on these findings, 7 of changnan-jeot or gaiyang-jeot products were confirmed by multiplex PCR and real-time PCR, and valid results were confirmed in all samples.

• Journal of KIISE
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• v.44 no.1
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• pp.107-113
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• 2017

Real-time multicasting is a packet transmission scheme ensuring that multiple destinations receive a packet within the desired time line. In wireless sensor networks, a packet can be delivered to a limited distance under a given deadline, since the end-to-end delay tends to be proportional to the end-to-end physical distance. Existing real-time multicasting protocols select the distance between the source and the furthest destination as the distance limitation and construct a multicasting tree guaranteeing delivery paths to each destination within the distance limitation. However, the protocols might lead to real-time delivery failures and energy efficiency degradation due to the fixed distance limitation. In this study, we proposed a real-time multicasting protocol using time deadline. The proposed protocol obtains the maximum transmittable distance with a given time deadline and subsequently constructs a multicasting tree using the maximum transmittable distance. The form of the multicasting tree varies according to the given time deadline to trade off the energy efficiency against the real-time delivery success ratio. The simulation results showed that the proposed scheme is superior to the existing protocols in terms of energy efficiency and real-time delivery success ratio under various time deadlines.

• Transactions of the Korean Society of Automotive Engineers
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• v.14 no.4
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• pp.192-198
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• 2006

The real-time simulator of a turning-type sluice gate actuated by the hydraulic cylinders is developed using a PC and a visual C++ program language. The real-time simulator receives the directional control valve signal selected by the operator using the mouse, updates the state variables of the turning-type sluice gate system responding to the control signal, and draws the moving figures of the sluice gate, cylinder, reserved water every drawing time on the PC monitor. Also, the operator can observe the sluice gate angle, cylinder force, cylinder pressures, and hydraulic power representing the operation of sluice gate system through the PC monitor every drawing time. The simulator can be a very useful tool to design and improve the turning-type sluice gate system.

• Journal of the Korean Institute of Intelligent Systems
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• v.8 no.6
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• pp.91-98
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• 1998

A DEDS is a system whose stated change in response to the occurrence of events from a predefined event set. A major difficulty in developing analytical results for the system is the lack of appropriate modeling techniques. In this paper, we consider the modeling and control problem for Discrete Event Dynamic Systems(DEDS) in the Temporal Logic framework(TLF) which have been recently defined. The traditional TLF is enhanced with time functions for real time control of Discrete Event Dynamic Systems. A sequence of event which drive the system from a given initial state to a given final state is generated by pertinently operating the given plants. This paper proposes the use of Real-time Temporal Logic as a modeling tool for the analysis and control of DEDS. An given example of fixed-time traffic control problem is shown to illustrate our results with Real-time Temporal Logic Framework.

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.842-847
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• 2005

This paper proposes a novel real-time EMG pattern recognition for the control of a multifunction myoelectric hand from four channel EMG signals. To cope with the nonstationary signal property of the EMG, features are extracted by wavelet packet transform. For dimensionality reduction and nonlinear mapping of the features, we also propose a linear-nonlinear feature projection composed of PCA and SOFM. The dimensionality reduction by PCA simplifies the structure of the classifier, and reduces processing time for the pattern recognition. The nonlinear mapping by SOFM transforms the PCA-reduced features to a new feature space with high class separability. Finally a multilayer neural network is employed as the pattern classifier. We implement a real-time control system for a multifunction virtual hand. From experimental results, we show that all processes, including virtual hand control, are completed within 125 msec, and the proposed method is applicable to real-time myoelectric hand control without an operation time delay.

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.1836-1839
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• 2005

We have developed Ethernet based real-time communication systems called "Vnet/IP" for DCS which is the control system for process automation. This paper describes the features and the technologies of the ASIC which is utilized in the communication interface hardware for Vnet/IP. Vnet/IP has been developed for mission-critical communications. Hence it has real-time feature, high reliability and precise time synchronization capability. At the same time, it is able to deal with standard protocols without influence on mission-critical communications. The communication interface hardware has a host interface and dual redundant network interfaces. The host interface can be chosen PCI-bus or R-bus which is the proprietary internal bus developed for the high reliable redundant controller. Each network interface is a RJ45 connection with 1Gbps maximum in compliance with IEEE802.3.