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http://dx.doi.org/10.9721/KJFST.2011.43.1.119

Comparison of Real-Time PCR and Culture Methods for Detection of Campylobacter jejuni in Various Foods  

Chon, Jung-Whan (Department of Public Health, College of Veterinary Medicine, Konkuk University)
Hyeon, Ji-Yeon (Department of Public Health, College of Veterinary Medicine, Konkuk University)
Hwang, In-Gyun (Food Microbiology Division, Korea Food and Drug Administration)
Kwak, Hyo-Sun (Food Microbiology Division, Korea Food and Drug Administration)
Han, Jeong-A (Food Microbiology Division, Korea Food and Drug Administration)
Kim, Moo-Sang (Epidemiology Team, Seoul Metropolitan Government Research Institute of Public Health and Environment)
Kim, Jong-Hyun (Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health)
Song, Kwang-Young (Department of Public Health, College of Veterinary Medicine, Konkuk University)
Seo, Kun-Ho (Department of Public Health, College of Veterinary Medicine, Konkuk University)
Publication Information
Korean Journal of Food Science and Technology / v.43, no.1, 2011 , pp. 119-123 More about this Journal
Abstract
In this study, performances of culture methods using two selective media and real-time PCR were evaluated for detection of Campylobacter jejuni (C. jejuni) in various food samples. Sausage, ground beef, and radish sprouts inoculated with C. jejuni were enriched in Hunt broth and then streaked onto modified cefoperazone charcoal deoxycholate agar and Preston agar, followed by incubation under microaerobic conditions. The enriched Hunt broth (1 mL) was used in real-time PCR assay. No statistical differences were observed in sensitivity among the two selective media and real-time PCR for sausage and ground beef. However, the number of positives by real-time PCR in radish sprouts was much higher than the two selective media (p<0.05). It appears that real-time PCR could be used as an effective screening tool to detect C. jejuni, particularly in foods with a high number of background microflora such as fresh vegetables.
Keywords
Campylobacter jejuni; real-time PCR; culture method; selective media;
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Times Cited By KSCI : 5  (Citation Analysis)
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