• Korean Journal of Agricultural Science
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• v.42 no.1
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• pp.7-13
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• 2015

Ninety genes randomly selected from tobacco whitefly (Bemisia tabaci) cDNA library was studied for selecting target gene in order to control of tobacco whitefly using TRV-VIGS vector (tobacco rattle virus-virus induced gene silencing vector) with RNAi. First of all, the occurrence of B. tabaci adult according to agro-infiltration of TRV was no significant difference. And that of TRV inserted tobacco whitefly cDNA showed a significant difference in each sample. P CV and N CV sample were more than 80% could be confirmed in 5 samples, for example, wh11, wh36, wh46, wh50 and wh71. Lastly, the occurrence of nymph and egg also showed a significant difference in each sample. That could be confirmed in 11 samples, for example, wh01, wh09, wh10, wh15, wh16, wh23, wh24, wh48, wh64 and wh66. In case of wh46, wh50 and wh71 sample could be confirmed that occurrence of B. tabaci adult was many, but occurrence of B. tabaci nymph and egg was a little. So sample showed a physioecological good effect to control of whitefly need to be investigated variation of gene expression in whitefly body using qRT-PCR through individual test.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.17 no.8
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• pp.673-682
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• 2007

Recently the trend of automobile industry is that IQS evaluation index against a sensitivity quality is increasing. To reduce rattle noise due to speaker sound at low frequencies, it is required the advanced technology analysis process of body structure. This paper optimized the design parameters of package tray panel according to the theoretical background about robust design and suggested the design guideline for resonance avoidance and the reduction of vibrational sensitivity considering the excitation frequency of woofer speaker. And this paper described the design process of a door module panel through the sensitivity analysis in case of the door speaker excitation. Finally, the analysis of the quality deviation using mother car is suggested to guarantee the stable characteristics of vehicle vibration in the early stage of vehicle development. These improvements can lead to shortening the time needed to develop better vehicles.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2014.10a
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• pp.791-795
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• 2014

In this paper, in order to impart the aging condition of the parts, by configuring the cycle of temperature from low temperature was performed by applying the aging conditions for vehicle cockpit module. The reason for the selected modules of the cockpit vehicle parts, because the joint occurrence typical components of the room component is a first module and ceiling cockpit module. After setting the excitation profile using the BSR exciter only that this is for the module degradation after the initial and grasp the change in the dynamic characteristics of the modules based on the before and after deterioration may be made in the module, grasp the noise generating position I measured the noise and proximity. Was also visualized on the position of the joint is generated using a sound camera to objective results occurring where the joint is selected through subjective evaluation.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.65.1-65
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• 2003

In addition to the five well-characterized genes of Tobacco mosaic virus (TMV), this virus contains a sixth open reading frame (ORF6) that encodes a 4.8 kDa protein. TMV ORF6 overlaps the ORFs encoding the 30 kDa movement protein and the adjacent 17.5 kDa capsid protein. Although the 4.8 kDa protein could not be detected in vivo, alteration of the AUG codons of this ORF resulted in a mutant virus that attenuated the virulence of the mutated TMV in Nicotiana benthamiana, but not N. tabacum (tobacco). These sequence changes did not affect either the replication or movement of the mutated TMV. Expression of TMV ORF6 from the virus expression vector Potato virus X (PVX) intensified the virulence of this virus in N. benthmiana, but not tobacco, while expression of TMV ORF6 from the virus expression vector Tobacco rattle virus enhanced the pathogenicity observed in both N. benthamima and tobacco. Thus, the TMV ORF6 is a host- and virus-specific. virulence factor. However, two separate assays indicated that the TMV 4.8 kDa protein was not a suppression of RNA silencing. A fusion protein formed between the TMV 4.8 kDa protein and the green fluorescent protein was expressed from the PVX vector and localized to plasmodesmata. Possible roles of the 4.8 kDa protein in pathogenicity will be discussed

• Korean Journal of Hospice Care
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• v.2 no.1
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• pp.75-86
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• 2002

Prupose: The purpose of this study was to identify signs and symptoms of hospice patients during their dying processes. Method: The subjects of this study were 76 patients who received hospice care services in 2 different hospice care programs in Seoul area. Data was obtained from January, 1999 to June, 2001 by hospice nurse's observation, interviews with patient's primary caregiver and hospice volunteers, patient's self report, nursing records and questionnaires which had been developed by selecting from various references and refining them based on the result of preliminay studies. The collected data were analyzed with the SPSS PC+ program and content analysis. Results: Mean lengths of hospice patient's dying process were 3.6 days. Physical signs and symptoms of hospice patients during their dying processes were 'increased sleep'(89.5%), 'decreased oral intake of food'(88.2%) and 'liquids'(86.8%), 'change of respiration'(82.9%), 'decreased urine output'(80.3%), 'cold extremities'(69.7%), 'death rattle'(67.1%), 'cyanosis'(57.9%), 'restlessness'(55.3%), etc. And psychological, spiritual and social signs and symptoms were 'decreased interpersonal relationships'(61.8%), 'panorama of memories from childhood'(60.5%), 'experiences as like hallucination'(56.5%), 'saying bye with family'(69.7%), 'forgiveness', 'make a will', etc. Experiences as like hallucination were seemed not as dream but reality were shown by 43 dying patients. They had experienced to see and to talk with their deceased mother(18.6%) and/or relatives(30.2%), angels(20.9%), heaven(11.6%), old house in which they had lived, someone from the world beyond with black clothes, etc. Conclusion: The above results indicate that death of hospice patients is ongoing process with dying signs and symptoms during several days contains not only in physical aspects but also among psychological, spiritual and social aspects including family dynamics.

• The Plant Pathology Journal
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• v.28 no.1
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• pp.40-48
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• 2012

$Potato$ $virus$ $X$ (PVX) replication is precisely regulated by regulatory viral sequences and by viral and/or host proteins. In a previous study, we identified a 54-kDa cellular tobacco protein that bound to a region within the first 46 nucleotides (nt) of the 5' non-translated region (NTR) of the viral genome. Optimal binding was dependent upon the presence of an ACCA sequence at nt 10-13. To identify host factors that bind to 5' NTR elements including AC-rich sequences as well as stemloop 1 (SL1), we used northwestern blotting and matrixassisted laser desorption/ionization time-of-flight mass spectrometry for peptide mass fingerprinting. We screened several host factors that might affect PVX replication and selected a candidate protein, $Nicotiana$ $tabacum$ WRKY transcription factor 1 (NtWRKY1). We used a $Tobacco$ $rattle$ $virus$ (TRV)-based virus-induced gene silencing (VIGS) system to investigate the role of NtWRKY1 in PVX replication. Silencing of $WRKY1$ in $Nicotiana$ $benthamiana$ caused lethal apical necrosis and allowed an increase in PVX RNA accumulation. This result could reflect the balancing of PVX accumulation in a systemic $N.$ $benthamiana$ host to maintain PVX survival and still produce a suitable appearance of mosaic and mottle symptoms. Our results suggest that PVX may recruit the WRKY transcription factor, which binds to the 5' NTR of viral genomic RNA and acts as a key regulator of viral infection.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.23 no.3
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• pp.240-248
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• 2013

Torsional vibrations, such as the gear rattle of the manual transmission in vehicle systems, are correlated with the firing stroke from the engine. These vibro-impacts can be examined based upon linear time-invariant analysis. In order to understand the gear dynamics, a specific manual transmission with a front-engine front-wheel drive configuration is investigated. A method to reduce the degrees of freedom is suggested based upon the eigensolutions and frequency response functions, which will lead to the development of an efficient matrix size. The dynamic characteristics of single- and dual-mass flywheels are then compared. The effect of the dual-mass flywheel is investigated based upon the mobility analysis, which will lead to understanding of the concepts for avoiding vibro-impacts. A linear time-invariant system model is examined by employing the effective clutch stiffness from a two-stage clutch damper. Thus, the relationship between the dynamic characteristics and the clutch damper can be predicted by assuming a combination of different stage stiffness levels.

• The Journal of the Korea institute of electronic communication sciences
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• v.13 no.2
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• pp.473-480
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• 2018

In 2012, a new energy capturing method called TENG was suggested for energy harvesting applications. The TENG which captures electric energy in forms of friction or vibration has been researched as a new energy harvesting generation device. However, TENG works on rather high voltage and yields relatively low current, and this requires additional energy conversion and saving methods with either in semiconductive elements or circuitry for its application. Irregular generation from vibration sources rattle under 5Hz especially requires empirical studies. In this article, we suggest a electricity generation platform with energy storage methods. The platform is mounted on large sized animals, and the generation is actively monitored and controlled via Bluetooth-Low Energy to verify the platform.

• Research in Plant Disease
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• v.7 no.2
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• pp.80-85
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• 2001

This study was carried out to examine tne incidences of virus diseases in lily plants cultivated in highland areas, and to develop an effective detection method. Viral symptoms on lilies in the highland areas were differentiated into mosaic, crinkle, mottle, stripe and line pattern. The distribution of symptoms on infected plants was 43.8% of mosaic, 29.2% of crinkle, and 10.9% of mottle symptoms. Six viruses such as Lily symptomless vires(LSV), Cucumber mosaic virus (CMV), Lily mottle virus (LMoV), Lily virus X (LVX, Potexvirus), Tabacco mosaic virus (TMV,Tobamovirus), and Tabacco rattle virus (TRV,Tobravirus) were detected from the infected lilies. Infection rate of Lilium oriental (cvs. Casablanca and Marcopolo) was 2~4 times higher than that of L. asiatic (cvs. Solemio and Prato). Virus detection on lilies by one-step RT-PCR (by using reverse transcription and polymerase chain reaction simultaneously) was more rapid rapid and reliable than by the conventional RT-PCR method.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.8
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• pp.4776-4783
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• 2014

Many studies have examined the reduction of primary noise sources, but quality-related noise, such as BSR, is rarely studied. This study describes the quantitative BSR test method using a multi-axial simulator. The sine sweep test was conducted to detect the system resonance and its relation to BSR noise with high frequency. This method is applied to the seat frame with/without the vibration durability test. The results showed that the $1^{st}$ lateral resonance leads to higher BSR frequency noise. In addition, the reduction of the lateral mode system stiffness after the durability test results in a decrease in the BSR noise in sine sweep test mode.