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Anti-Obesity Effect of Enterococcus faecalis MD366 isolated from Raw Milk in Diet-Induced Obese Mice (원유에서 분리한 Enterococcus faecalis MD366가 고지방식이를 급여한 마우스의 비만 억제에 미치는 영향)

  • Park, Sun-Young;Cho, Seong-A;Han, Noo-Ri;Seong, Ki-Seung;Lim, Sang-Dong
    • Journal of Dairy Science and Biotechnology
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    • v.32 no.2
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    • pp.163-167
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    • 2014
  • This study aimed to investigate the anti-obesity effects of Enterococcus faecalis MD366, isolated from raw milk, in diet-induced obese mice. To examine the effect, male C57BL/6J mice were fed for six weeks on three different diets, i.e., a normal diet and orally administrated saline solution (ND), a high-fat diet and orally administrated saline solution (HFD), and a high-fat diet and orally administrated E. faecalis MD366 ($10^9CFU/day$) in saline solution (HFD+MD366). After six weeks, the rate of increase in body weight was 18.1% lower in the HFD+MD366 group compared to that in the HFD group. In addition, the weight of epididymal fat pad in the HFD+MD366 group was lower than that in the HFD group. The average levels of triglycerides, total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) were slightly reduced in the HFD+MD366 group compared to those in the HFD group. However, there were no significant differences between the groups. Measuring the adipocytes revealed that the percentage of adipocytes with a size of $2,000{\mu}m^2$ was higher than the percentages of other size classes in ND and HFD+MD366 groups, while the percentage of adipocytes larger than $5,000{\mu}m^2$ was highest in the HFD group. The mean adipocyte size in the HFD+MD366 group was smaller than that in the HFD group.

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Development of Economic Culture System Using Wastewater for Microalgae in Winter Season (폐수를 이용한 겨울철 경제적 미세조류 배양 시스템의 개발)

  • Lee, Sang-Ah;Lee, Changsoo;Lee, Seung-Hoon;An, Kwang-Guk;Oh, Hee-Mock;Kim, Hee-Sik;Ahn, Chi-Yong
    • Korean Journal of Environmental Biology
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    • v.32 no.1
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    • pp.58-67
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    • 2014
  • The outdoor mass cultivation is not possible for microalgae in Korea all year round, due to cold winter season. It is not easy to maintain proper level of productivity of microalgae even in winter. To prevent a drastic decrease of temperature in a greenhouse, two layers were covered additionally, inside the original plastic layer of the greenhouse. The middle layer was made up of plastic and the inner layer, of non-woven fabric. Acrylic transparent bioreactors were constructed to get more sunlight, not only from the upper side but also from the lateral and bottom directions. In winter at freezing temperatures, six different culture conditions were compared in the triply covered, insulated greenhouse. Wastewater after anaerobic digestion was used for the cultivation of microalgae to minimize the production cost. Water temperature in the bioreactors remained above $10^{\circ}C$ on average, even without any external heating system, proving that the triple-layered greenhouse is effective in keeping heat. Algal biomass reached to 0.37g $L^{-1}$ with the highest temperature, in the experimental group of light-reflection board at the bottom, with nitrogen and phosphorus removal rate of 92% and 99%, respectively. When fatty acid composition was analyzed using gas-chromatography, linoleate (C18 : 3n3) occupied the highest proportion up to 61%, in the all experiment groups. Chemical oxygen demand (COD), however, did not decrease during the cultivation, but rather increased. Although the algal biomass productivity was not comparable to warm seasons, it was possible to maintain water temperature for algae cultivation even in the coldest season, at the minimum cost.

A Study on the Adsorption of Rare Earth Elememts by Raw and Crosslinked Chitosan (천연 및 가교 키토산에 의한 희토류 원소의 흡착과 회수에 관한 연구)

  • Cho, Sung-Ill;Choi, Jong-Moon;Kim, Young-Sang;Lee, Sueg-Geun
    • Analytical Science and Technology
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    • v.17 no.2
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    • pp.108-116
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    • 2004
  • The adsorption characteristics of raw and crosslinked chitosan for rare earth elements (REEs) have been studied. The range of optimum pH for the maximum adsorption was observed: pH 4.5~5.5 for $Nd^{3+}$, $Tm^{3+}$ on raw and crosslinked chitosan; pH 4.0~5.5 for $La^{3+}$ and $Ce^{3+}$ on crosslinked chitosan and pH 2.0 for those on raw chitosan. The adsorption rate of REE at pH 4.0 has been found in the order of $Er^{3+}$ > $Gd^{3+}$ > $Yb^{3+}$ > $Nd^{3+}$ > $Lu^{3+}$ > $Eu^{3+}$ > $Tm^{3+}$ > $Ho^{3+}$ > $Dy^{3+}$ > $La^{3+}$ > $Ce^{3+}$ > $Y^{3+}$ > $Pr^{3+}$ in single metal system and that of $Lu^{3+}$ > $Yb^{3+}$ > $Tm^{3+}$ > $Dy^{3+}$ > $Ho^{3+}$ > $Er^{3+}$ > $Eu^{3+}$ > $Gd^{3+}$ > $Nd^{3+}$ > $Y^{3+}$ > $La^{3+}=Ce^{3+}=Pr^{3+}$ in multi metal system. In the competitive adsorption of multi metal system, the amount of metal adsorption generally increased with increasing atomic number and with decreasing ionic radius. On the adsorption studies of metal ions on chitosan, the time of equilibrium adsorption which was reached at the maximum adsorption was about 5 hours. 83~95 % for $Nd^{3+}$ ion and 90~106 % for $Tm^{3+}$ ion, were recovered from the crosslinked chitosan.

The Effects of Dietary Supplementation of Feather Meal Digests on the Performances and Muscular Taurine Contents in Broiler Chickens (육계 사료내 우모 Digest 첨가시 생산성과 계육내 Taurine 함량에 미치는 영향)

  • Lee, S.M.;Lim, H.S.;Kim, W.Y.;Paik, I.K.
    • Journal of Animal Science and Technology
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    • v.46 no.5
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    • pp.753-762
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    • 2004
  • its digests on the performance of broiler chicks and taurine content in broiler meat. A total of 100 broiler chickens were assigned to five dietary treatments; Control, PM diet(FM), NaOH treated FM diet(NaOH-FM), HN03 treated FM diet(HN03 - FM) and 0.5% synthetic taurine supplemented diet(Taurine). Treated diets were supplemented with FM or FM digests at the level of 5% to the control diet Treated diets were fed during the last 3wks of 6wks feeding. During the finisher period, weight gain of chicks fed FM treatments tended to decrease in general. Feed intakes of FM and Taurine were significantly lower than the control but were not significantly different from NaOH - FM or HN03- PM. Feed conversion rate(feed intake/gain) of NaOH-FM was significantly higher than those of FM and HNOJ - FM but not different from those of the control and Taurine. Taurine content of the control was highest in heart muscle(1482 J1.g/g) followed by leg muscle(778f.lg/g) and breast muscle(79 J1.g/g). Taurine contents of leg and breast muscle were significantly(P< 0.01) affected by treatments but that of heart muscle was not. Taurine content of Taurine treatments was highest in both leg and breast muscle. Taurine content of leg muscle was increased 170"10 by Taurine supplementation, 123% by FM supplementation, 122% by NaOH - FM supplementation and 63% by HNOJ- FM supplementation compared with control. Taurine content of breast muscle of Taurine treatment was 246% higher than the control, but other treatments were not different from the control. In conclusion, supplementation of feather meal at 5% level of broiler diet can enrich taurine content of leg muscle. Chemical treatments of FM were not effective in improving taurine enrichment of broiler meat.

Estimation of Genetic Parameters for Economic Traits and Profit by Milk Production of Holstein Dairy Cattle in Korea (국내 Holstein종 젖소의 경제형질과 착유량에 따른 소득의 유전모수 추정)

  • Noh, Jae-Kwang;Choi, Yun-Ho;Cho, Kwang-Hyun;Choi, Tae-Jeong;Na, Seung-Hwan;Cho, Ju-Hyun;Kim, Jin-Hyung;Shin, Ji-Sub;Do, Chang-Hee
    • Journal of Animal Science and Technology
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    • v.54 no.4
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    • pp.275-282
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    • 2012
  • The data including milk yields, fat and protein percent for 628,395 heads collected by National Agricultural Cooperative Federation, 15 type traits and final score for 62,262 heads collected by Korea Animal Improvement Association, which were born in 1998 to 2004, and net profits calculated from milk price and raising expenses of individuals were used to estimate genetic parameters. The highest positive genetic correlation, 0.81, was shown between body depth (BD) and loin strength (SR). Genetic correlations between body depth (BD) and udder depth (UD), front teat placement (TP) and front teat length (TL) were -0.23, which were lowest among the linear type traits. Furthermore, medium level of negative genetic correlations were shown the milk yield with milk contents rate traits. Mostly low level of positive genetic correlations were shown between the milk traits and linear score traits except milk yield and stature. Most of the genetic correlations of between the linear score traits and net profit were low level of positive or negative genetic correlations. Among the genetic correlations, body depth (BD), angularity (DF) and rear attachment width (UW), and final score (FS) with net profit were high as 0.17, 0.17, 0.18 and 0.18, respectively. Finally all of the genetic correlations between net profit and milk traits were positive and higher than the linear traits with positive genetic correlations. The results of this study suggest that net profit has been related with the linear traits, such as body depth (BD), angularity (DF) and rear attachment width (UW) traits, and furthermore, milk traits including yield and contents rates influence positively and greatly on net profit.

Genotype Frequencies of the Sex-Linked Feathering and Their Phenotypes in Domestic Chicken Breeds for the Establishment of Auto-Sexing Strains (자가성감별 계통 조성을 위한 국내 토종 닭의 깃털 조만성 양상과 유전자형 빈도)

  • Sohn, Sea-Hwan;Park, Dhan-Bee;Song, Hae-Ran;Cho, Eun-Jung;Kang, Bo-Seok;Suh, Ok-Suk
    • Journal of Animal Science and Technology
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    • v.54 no.4
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    • pp.267-274
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    • 2012
  • The method of sexing based on differences in the rate of feather growth provides a convenient and inexpensive approach. The locus of feather development gene (K) is located on the Z chromosome and can be utilized to produce phenotypes that distinguish between the sexes of chicks at hatching. To establish the auto-sexing native chicken strains, this study analyzed the genotype frequency of the feathering in domestic chicken breeds. The method of classification of slow- and rapid-feathering chickens was also investigated. In the slow-feathering chicks, the coverts were either the same length or longer than the primary wing feathers at hatching. However, the rapid-feathering chicks had the primary wing feathers that were longer than the coverts. The growth pattern of tail feather also distinctively differed between the rapid- and slow-feathering chicks after 5-days. The accuracy of wing feather sexing was about 98% compared with tail sexing. In domestic chicken breeds, Korean Black Cornish, Korean Rhode Island Red, and Korean Native Chicken-Red had both dominant (K) and recessive ($k^+$) feathering genes. The other breeds of chickens, Korean Brown Cornish, Ogol, White Leghorn, Korean Native Chicken-Yellow, -Gray, -White and -Black had only the recessive feathering gene ($k^+$). Consequently, feather sexing is available using the domestic chicken breeds. Establishing the maternal stock with dominant gene (K-) and paternal stock with recessive gene ($k^+k^+$), the slow-feathering characteristic is passed from mothers to their sons, and the rapid-feathering characteristic is inherited by daughters from their fathers.

Utility-Based Video Adaptation in MPEG-21 for Universal Multimedia Access (UMA를 위한 유틸리티 기반 MPEG-21 비디오 적응)

  • 김재곤;김형명;강경옥;김진웅
    • Journal of Broadcast Engineering
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    • v.8 no.4
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    • pp.325-338
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    • 2003
  • Video adaptation in response to dynamic resource conditions and user preferences is required as a key technology to enable universal multimedia access (UMA) through heterogeneous networks by a multitude of devices In a seamless way. Although many adaptation techniques exist, selections of appropriate adaptations among multiple choices that would satisfy given constraints are often ad hoc. To provide a systematic solution, we present a general conceptual framework to model video entity, adaptation, resource, utility, and relations among them. It allows for formulation of various adaptation problems as resource-constrained utility maximization. We apply the framework to a practical case of dynamic bit rate adaptation of MPEG-4 video streams by employing combination of frame dropping and DCT coefficient dropping. Furthermore, we present a descriptor, which has been accepted as a part of MPEG-21 Digital Item Adaptation (DIA), for supporting terminal and network quality of service (QoS) in an interoperable manner. Experiments are presented to demonstrate the feasibility of the presented framework using the descriptor.

Influence of Yohimbine on the Central Dopaminergic Regulation of Renal Function (신장기능의 중추 Dopamine성 조절에 미치는 Yohimbine의 영향)

  • Kook, Young-Johng;Kim, Kyung-Keun;Cho, Kang-Seon;Min, Byung-Kap
    • The Korean Journal of Pharmacology
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    • v.22 no.2
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    • pp.79-87
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    • 1986
  • Recently it has been shown that central dopaminergic system regulates the renal function and that intracerebroventricularly (icv) administered dopamine (DA) produces antidiuresis and antinaturiuresis, resembling icv norepinephrine, and evidence has been accumulated which would suggest the involvement of adrenergic system in the DA effects. It was attempted therefore in this study to see whether the DA effect is influenced by pretreatment of yohimbine which is known as a specific ${\alpha}_2-adrenoceptor$ antagonist. Yohimbine produced, when given icv in doses of $100\;{\mu}g/kg$, marked antidiuresis and antinatriuresis along with decreases in renal perfusion and glomerular filtration. DA, in doses of $15\;{\mu}g/kg$, also produced antidiuresis and antinaturiuresis. However, after yohimbine-pretreatment DA $15\;{\mu}g/kg$ improved renal hemodynamics, and electrolyte excretion and urine flow rate transiently increased. With $150\;{\mu}g/kg$ DA, the antidiuresis was more marked in the control group. But the yohimbine-pretreated animals responded with marked diuresis and natriuresis, sodium excretion increasing more than three-fold, which lasted for 20 minutes. $K^+-excretion$, osmolar clearance as well as free-water reabsorption increased. Renal hemodynamics improved partly. Apomorphine, a DA agonist, when given icv in doses of $150\;{\mu}g/kg$, produced diuresis and naturiuresis, concomitant with increased renal hemodynamics. Yohimbine-pretreatment however did not abolish the apomorphine-induced diuresis and naturiuresis. Antidiuresis and antinatriuresis elicited by norepinephrine, $10\;{\mu}g/kg$, was not affected by yohimbine-pretreatment. These results indicate that the renal effects of icv DA is not so simple as those of norepinephrine, and the diuretic natriuretic cffect which had been masked by the hemodynamic effect becomes manifest only when the decreases in hemodynamics were removed by the pretreatment of yohimbine. It was further suggested that those DA receptors which mediate the natriuretic response to icv DA is not affected by yohimbine, whereas those receptors involved in the decrease in renal hemodvnamics are blocked by yohimbine. And the possibility of involvement of adrcnergic system in the DA action is not substantiated.

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Leaching behaviour of the herbicide mefenacet in the soil columns (토양 column중 제초제 mefenacet의 용탈)

  • Kim, Sung-Min;Kwon, Jeong-Wook;Ahn, Ki-Chang;Cho, Il-Kyu;Kyung, Kee-Sung;Lee, Jae-Koo
    • The Korean Journal of Pesticide Science
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    • v.7 no.3
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    • pp.176-181
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    • 2003
  • The leaching behaviour of [aniline-$^{14}C$]mefenacet in soil was investigated using glass columns (5 cm I.D. $\times$ 30 cm. H) packed with two types of soils with different physicochemical properties. $^{14}C$-Mefenacet (8.33 kBq) and mefenacet (in total, 1.05 mg/kg) were treated onto soil columns and rice plants (Oryza sativa L.) were grown for 17 weeks on these columns. Leachates from the columns were collected at the rate of 122.5 mL per week. $^{14}C$-Activities leached from soil A (OM, 3.1%; CEC, 86 mmol(+)/kg; texture, loam) columns with and without rice plants were 1.95 and 4.19% of the originally applied, whereas those from soil B (OM, 1.3%; CEC, 71 mmol(+)/kg; texture, loam) were 2.69 and 7.05%, respectively. These results indicated that larger amounts of $^{14}C$ were percolated from soil B with less organic matter and from the columns without vegetation. $^{14}C$-Activities absorbed by rice plants from soil A and B were 8.95 and 8.47%, respectively, most of which remained in the root and shoot excluding unhulled grains and ears without grains. $62\sim73%$ of the originally applied $^{14}C$ remained in the depth of $0\sim5cm$ in soil. The mass balance indicated that the losses by volatilization and/or mineralization amounted to $3.4\sim9.2%$ of the originally applied. $^{14}C$-Radioactivities in the aqueous phase of the leachates ranged from 59.4 to 97.7% of the radioactivities in leachates, showing the fast transformation of mefenacet to the polar metabolites.

Several causes of non virus-induced mosaic symptom on potato leaves and its induction by herbicides (감자 이상모자이크증상의 몇 가지 발생원인 및 제초제에 의한 증상 유기)

  • Kwon, Min;Hahm, Young-Il;Kim, Hyun-Jun;Yiem, Myoung-Soon
    • The Korean Journal of Pesticide Science
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    • v.5 no.2
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    • pp.45-50
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    • 2001
  • In recent, non virus-induced mosaic symptoms(NVMS) on potato leaves were observed in the seed potato fields, and its incidence rate was $5{\sim}20%$ nationwide. It made difficult to rogue out virus-infected plants, and caused much arguments between seed potato production farmers and seed potato inspectors. The objectives of these experiments were to find out the causes of NVMS, and also to induce mosaic symptom(phytotoxicity) on potato plants by treatment of several herbicides. No significant correlations were found between incidence rates of NVMS and values from soil analyses; soil pH, soil EC, organic matter content, and contents of inorganic constituents($P_2O_5,\;NO_3$, Ca, Mg, K) in the soil around the potato planted. The examinations by ELISA, virus indicator plants, and TEM showed that NVMS on potato leaves was not caused by the viruses infection. But, the use of herbicides could induced the NVMS on potato leaves. The incidence rates of potato treated with pendimethalin linuron of 400 mL/10 a, pendimethalin of 200 mL/10 a, pendimethalin.oxadiazon of 300 mL/10 a, and control were 61.1%, 47.2%, 19.4%, and 1.4%, respectively. Based on these results, we confirmed that the treatment of pendimethalin alone and in mixture with other herbicides were the reason of NVMS on potato leaves. The yields among test plots were similar except dicamba treated plot, which decreased by about 23% compared to control plot. When their progenies harvested in 1999 were planted in the following season, no symptoms of mosaic were observed.

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