• Journal of The Association for Neo Medicine
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• v.1 no.2
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• pp.15-30
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• 1996

In the study about the logical basis and interpreting methods of animal experiments using rats in traditional medicine, several conclusions are obtained and summarized as following. 1. The logical basis of the animal experiments in traditional medicine is the essential homogeneity between human and rat defined as various transformation of one Qi(一氣) packed the cosmos. 2. Morphologically, the rat has abundant Yin(陰) and less Yang(陽) in most of anatomical characteristics. 3. Physiologically, the rat has unstable heart and mild temper with good fertility, which shows the features of Yin animal. 4. Pathologically, the rat shows the pathology of injury of viscera by stresses(氣激傷臟) and pathological transformations of internal water(痰飮水濕) mainly. 5. Constitutionally, the rat is alike to water type man(水形人) or Soeumin(小陰人) out of Yin Ren(陰人). 6. So, the rat is the proper experimental animal for diseases of sputum and water, emotional stimulations, endocrine system, heart, kidney, Yin syndrome(陰證) etc..

• The Journal of Korean Physical Therapy
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• v.11 no.2
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• pp.115-122
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• 1999

This experiment was carried out to investigate the bombesin immunoreactivity in suprachiasmatic nucleus in rat and Mongolian gerbil hypothalamus after colchicine treatment and analyze the morphological difference between rat and Mongolian gerbil which is focused for experimental animal model of neuronal and circulatory diseases. The results were as followings. 1. The shape of suprachiasmatic nucleus was triangle in rat, but oval or kidney-shape in Mongolian gerbil 2. The suprachiasmatic nucleus devided into ventrolateral portion and dorsomedial portion in rat, but dorsolateral portion and ventromedial portion or superior portion and inferior portion in Mongolian gerbil. 3. The area of suprachiasmatic nucleus of rat was greater than one of Mongolian gerbil. 4. The bombesin immunoreactivity showed after colcichine treatment in rat and Mogolian gerbil suprachiasmatic nucleus. 5. The bombesin immunoreactivity was stronger in ventrolateral portion than in dorsomedial portion of suprachiasmatic nucleus in rat, but in ventromedial or inferior portion than in dorsolateral or superior portion of suprachiasmatic nucleus in Mongolian gerbil. 6. The bombesin immunoreactivity showed at the oval, ellipsoid or triangular neurons and varicose nerve terminals in ventrorateral portion of rat, and only nerve terminals in dorsomedial portion of rat suprachiasmatic nucleus. But the bombesin immunoreativity didn't show at neurons of Mongolian gerbil suprachiasmatic nucleus.

• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.2
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• pp.92-98
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• 2000

Mesangial cell has several key roles in thee control of glomerular function: it partocipates in the regulation of glomerular filtration rate, macromolecular clearance, and as both a source and target of numerous hormones and autocrines. Many of these insights into mesangial cell function have been obtained by studying mesangial cells in culture. However, no suitble cell lines have established yet. We here reported the immortalization of rat kidney glomeruar mesangial cell by transfection of E6 and E7 genes of human papillomavirus type 16 (HPV-16) via electroporation and lipofection. The reslts showed that only electroporation could transfect the genes to mesangial cells and the transfected cells maintained the viability for longer than 6 months. Fluorescence microscopic observation showed that cellular contractility and phagocytosis, which are the two main phenotypes of mesangial cells with rat glomerular epithelial cells showed that the growth of mesangial dells was suppressed by epithelial cell, but the growth of epithelisl cells was enhanced by mesangial cells. Moreover, Such results may imply that the glomerular cell-cell interaction plays an important role in the regulation of cell proliferation and differentiation.

• Biomolecules & Therapeutics
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• v.1 no.2
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• pp.220-225
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• 1993

Effect of BR-900317 on the angiotensin I-induced pressor response in pithed rats and the effects of its single oral administration on plasma angiotensin converting enzyme (ACE) activities in normotensive rats and on the cardiovascular system in hypertensive model rats (SHR, RHR), were compared with those of captopril. BR-900317 attenuated the angiotensin I-induced pressor effects in pithed rats. In a single oral dose administration study, BR-900317 inhibited the plasma ACE activities in a dose-dependent fashion. Duration of the action of BR-900317 was similar to that of captopril. BR-900317 produced antihypertensive effect in spontaneously hypertensive rats and dose-dependent antihypertensive effect in 2-kidney Goldblatt hypertensive rats without affecting heart rate. These results suggest that the main mechanism of the antihypertensive effect of BR-900317 is the suppression of angiotensin II production due to the inhibition of the ACE.

• The Journal of Internal Korean Medicine
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• v.32 no.2
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• pp.278-287
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• 2011

Objectives : The object of this study was to observe the nephroprotective effects of Sojongchobisunki-tang (SCST), which has traditionally been used as Korean medicine for treating various renal diseases, on cisplatin-induced rat acute renal failure. Methods : Three different dosages of SCST were orally administered once a day for 23 days before cisplatin treatment (5 mg/kg, single intraperitoneally administered) and 5 days after cisplatin treatment (once a day for 28 days). 6 groups, of 8 rats per group were used in the present study after 7 days of acclimatization. Changes of the body weight, kidney weight, serum BUN and creatinine levels were observed, as well as changes of the kidney MDA and GSH contents. The results were compared with captopril 100 mg/kg of which the effects on cisplatin-induced acute renal failures are already confirmed. Results : Acute renal failure induced by cisplatin were induced by oxidative stress and related lipid peroxidation in the present study. However, these acute renal failures and inhibition of antioxidant effects induced by cisplatin were dose-dependently reduced by treatment at all three different dosages of SCST extracts. Conclusions : This study suggests that SCST extracts showed favorable effects on the cisplatin-induced rat ARF.

• Natural Product Sciences
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• v.3 no.1
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• pp.59-70
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• 1997

A rat chemokine, cytokine-induced neutrophil chemoattractant-1 (CINC-1) has chemotactic and activating properties to neutrophils. Rat kidney epithelioid NRK-52E cells contained 4 ng/ml of ClNC-1 as a basal level and their CINC-1 production was significantly increased by stimulation with lipopolysaccharide (LPS) of E. coli. Maximal induction of ClNC-1 was 58 ng/ml when 3 ${\mu}g/ml$ of LPS was treated to the NRK-52E cells. Inhibitory effects on CINC-1 induction in LPS-stimulated NRK-52E cells by extracts prepared from herbal medicines and wild plants in Korea were analyzed. At the final concentration of 100 ${\mu}g/ml$ , 9 species out of 304 species of herbal extracts exhibited more than 50% of inhibition on the CINC-1 induction. The active extracts prepared from Artemisia argyi, Lythrum salicaria, Machilus thunbergii, Magnolia sieboldii, Nelumbo nucifera, Prunus persica, Rubus coreanus, Sanguisorba officinalis, and Tripterygium regelii have been sequentially fractionated to obtain methylene chloride, ethyl acetate, butanol, and aqueous layers. Among solvent fractions of the active herbal extracts, methylene chloride fractions of Artemisia argyi and Magnolia sieboldii exhibited the highest inhibitory effects on CINC-1 induction in LPS-stimulated NRK-52E cells.

• Korean Journal of Veterinary Pathology
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• v.1 no.1
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• pp.13-25
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• 1997

The anticarcinogenic effects of dimethyl disulfide(DMDS, methyl disulfide)and diallyl disulfide(DADA, allyl disulfide) were studied in a 28 weeks rat multi-organ carcinogenesis model. neoplastic and preneoplastic lesions were observed in the liver kidney thyroid gland esophagus duodenum colon, rectum and adrenal gland. Tesults showed that neoplastic lesions in the kidney liver and thyroid gland were inhibited by DADS but those in the liver and colon were enhanced by DMDS when compared to positive control group. incidence of neoplastic lesions in the other organs were not changed by DMDS or DADS exposure. While GST-p positive foci in the liver were increased by DMDS, DADS had no effect. There was no significant histopathological lesion in DMDS or DADS treated group without pretreatment with carcinogens.

• Korean Journal of Veterinary Pathology
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• v.1 no.2
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• pp.107-118
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• 1997

The anticarcinogenic effects of allyl methyl trisulfide(AMT) and methyl propyl disulfide(MPD) were studied in a 28 weeks rat multi-organ carcinogenesis model. Tumor incidence rate was decreased by AMT or MPD treatment comparing with the positive control. AMT treatment significantly decreased the incidence of neoplastic and preneoplastic lesions in the kidney thyroid gland urinary bladder alimentary tract lung and Zymbal's gland. MPD also inhibited incidence of noplastic and preneoplastic lesions in the liver kidney urinary bladder alimentary tract lung and Zymbal's gland but increased that in the thyroid gland. GST-p positive foci in the liver were slightly decreased by AMT or MPD. There was no significant histopathological lesions in AMT or MPD treated group without pretreatment of carcinogens.

• Proceedings of the Korean Society of Life Science Conference
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• 2007.10a
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• pp.75.2-75.2
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• 2007

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• The Korea Journal of Herbology
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• v.20 no.4
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• pp.53-60
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• 2005

Objectives : This study was carried out to determine if Dendrobii Herba have protective effect against cell injury induced by various toxic agents in rat kidney slices. Water(DWe) and methanol(DMe) extracts were prepared for this experiment. Methods : Cell injury was estimated by measuring lactate dehydrogenase(LDH). Lipid peroxidation was examined by measuring malondialdehyde, a product of lipid peroxidation. Results : DMe prevented the LDH release by $CCl_4$, menadione, tert-butyl hydroperoxide and mercury treatment in vitro in kidney slices, but DWe prevented the LDH release by $CCl_4$ and mercury. DMe also prevented reduction in GSH and lipid peroxidation induced by $CCl_4$ and mercury. Conclusion : Thus, DMe may have more powerful efficacy on anti-oxidative effects when compared with DWe. And further studies have to be followed concerned with extraction of Dendrobii Herba and its change of effects.