• 대한의생명과학회지
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• 제29권4호
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• pp.344-354
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• 2023

This study emphasizes the importance of early diagnosis and response to COVID-19, leading to the development of a rapid diagnostic kit using quantum dots. The research focuses on finely tuning bioconjugation with quantum dots to enhance the accuracy and sensitivity of COVID-19 diagnosis. We have developed a COVID-19 rapid diagnostic kit that exhibits a sensitivity more than 50 times higher than existing COVID-19 diagnostic kits. Quantum dots enable the accurate detection of COVID-19 viral antigens even at low concentrations, providing a rapid response in the early stages of infection. The COVID-19 quantum dot diagnostic kit offers quick analysis time, utilizing the quantum properties of particles to swiftly measure COVID-19 infection for immediate response and isolation measures. Additionally, this diagnostic kit allows for multiple analyses with ease, as multiple quantum dots can detect various antigens and antibodies simultaneously in a single experiment. This efficiency enhances testing, reduces sample requirements, and lowers experimental costs. The application of this diagnostic technology is anticipated in the future for early diagnosis and monitoring of other infectious diseases.

• 대한임상검사과학회지
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• 제43권1호
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• pp.1-5
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• 2011

The infection rate of syphilis is still increasing in the world especially in developing countries and the infection is often seen in large amounts of clinical specimens. For the diagnosis of this disease, Rapid Plasma Reagin (RPR)/Venereal Disease Research Laboratory (VDRL) has still been used as one of major primary methods to diagnose syphilis even though the test readings are somewhat subjective with high false positive rates. Recently, the automatic ARCHITECT Syphilis TP, which is based on the detection of the TP-specific antibodies, has been introduced in many laboratories. Therefore, the clinical assessment of the method is needed to provide primary diagnosis of syphilis at the moment. We evaluated 3 different manual rapid kits and ARCHITECT Syphilis TP comparing with RPR/FTA-ABS and analysed their diagnostic properties. From February 2006 to April 2008, 203 positive and 250 negative specimens, obtained from Chungbuk National University Hospital were used for the evaluation. In the evaluation between manual rapid kits, their specificities were as high as 99.2 ~ 99.6% while their sensitivities were observed with little differences; 98.0% (199/203) for Kit A, 96.6% (196/203) for Kit B, and 97.4% (197/203) for Kit S. In the case of ARCHITECT Syphilis TP test, it showed 100% specificity (250/250) and 98.5% sensitivity (249/250). Kappa values comparing with RPR/FTA-ABS were 0.978 for Kit A, 0.964 for Kit B and Kit S, and 0.987 for ARCHITECT Syphilis TP. From our evaluation, we found out that manual rapid tests and ARCHITECT Syphilis TP have very good clinical accuracies and high kappa agreements with RPR/FTA-ABS. Due to its automation and quick simultaneous diagnosis with another serological markers, we suggest that the ARCHITECT Syphilis TP is one of best suitable method for the primary diagnosis of syphilis and that it might be able to replace RPR method in the laboratories.

• Journal of Microbiology and Biotechnology
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• 제28권10호
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• pp.1683-1690
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• 2018

Accurate and rapid diagnosis of influenza infection is essential to enable early antiviral treatment and reduce the mortality associated with seasonal and epidemic infections. Immunochromatography is one of the most common methods used for the diagnosis of seasonal human influenza; however, it is less effective in diagnosing pandemic influenza virus. Currently, rapid diagnostic kits for pandemic influenza virus rely on the detection of nucleoprotein (NP) or hemagglutinin (HA). NP detection shows higher specificity and is more sensitive than HA detection. In this study, we time-dependently screened expression conditions, and herein report optimal conditions for the expression of recombinant nucleoprotein (rNP), which was 48 h after infection. In addition, we report the use of the expressed rNP in a rapid influenza diagnostic test (SGT i-flex Influenza A&B Test). We constructed expression vectors that synthesized rNP (antigen) of influenza A and B in insect cells (Sf9 cells), employed the purified rNP to the immunoassay test kit, and clearly distinguished NPs of influenza A and influenza B using this rapid influenza diagnostic kit. This approach may improve the development of rapid test kits for influenza using NP.

• 한국식품저장유통학회지
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• 제30권2호
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• pp.262-271
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• 2023

본 연구에서는 real-time PCR(SureTect^TM kit와 PowerChek^TM kit), LAMP(3M MDS), 선택 배지를 이용하여 즉석섭취식품에 존재하는 Salmonella spp.와 L. monocytogenes의 검출 능력을 비교 및 평가하고 식품 매트릭스가 real-time PCR의 결과에 미치는 영향을 조사하였다. 4가지 서로 다른 농도로 접종된 식품을 동일한 증균배지를 이용하여 증균 후 세 가지 방법으로 검출한 결과, real-time PCR, LAMP, 선택 배지에서 모두 양성으로 검출되어 인위적으로 접종된 식품에서의 검출 성능은 동등한 것으로 나타났다. 또한, 식품 매트릭스가 real-time PCR의 신속 검출에 미치는 영향을 조사한 결과, Salmonella spp.의 검출에서 샐러드가 다른 식품에 비해 Ct value가 유의적으로 높은 것으로 나타나, 섬유질이 풍부한 식품에 존재하는 Salmonella spp.의 검출을 위해서는 충분한 균질화와 균체의 탈리, 그리고 효율적인 DNA의 증폭이 필요함을 알 수 있었다. 반면, L. monocytogenes의 검출은 식품 매트릭스마다 상이하며 혼합적인 양상을 보였다. 현재의 식품공전 규정에서 식품에 존재하는 식중독균의 신속 검출을 위한 장비와 시약의 사용은 대부분 사용자의 선택에 의존하고 있다. 본 실험에서 real-time PCR로 사용된 SureTect^TM kit와 PowerChek^TM kit는 기존 real-time PCR kit의 대체재로서 사용이 가능할 것으로 판단되며, 또한, LAMP도 우수한 검출 성능을 보였기에 식품안전 관리 수단으로 활용될 가능성이 있음을 시사하고 있다.

• Journal of Microbiology and Biotechnology
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• 제24권3호
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• pp.427-430
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• 2014

We developed a novel immunochromatographic assay (ICA) (EZ-Step VanA rapid kit; Dinona, Korea) for the detection of VanA ligase from vancomycin-resistant enterococci (VRE). Of eight monoclonal antibodies screened by ELISAs, the VanA ligase ICA constructed with 1H9 plus 3G11 showed the greatest reactivity. The detection limit of the kit was $6.3{\times}10^6$ CFU per test. Of 127 vancomycin-resistant microorganisms, 100 vanA VRE were positive in the VanA ligase ICA, and 27 non-vanA vancomycin-resistant isolates were negative. These results were consistent with those of the PCR analyses. Thus, our ICA is a reliable and easy-to-use immunological assay for detecting VanA-producing VRE in clinical laboratories.

• 농업과학연구
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• 제39권3호
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• pp.421-425
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• 2012

식중독을 일으키는 대표적인 원인균인 살모넬라를 검출하기 위해 측방 유동형 간이진단 키트를 제작하였다. 또한, 제작된 간이진단 키트의 검출한계를 향상시키고 살모넬라 균수를 정량적으로 분석하기 위해 이미지 분석 시스템을 적용하였다. 그 결과 간이진단 키트의 검출한계는 $10^6\;cfu/mL$에서 $10^5\;cfu/mL$로 향상시킬 수 있었다. 살모넬라 균수의 정량적 분석을 위해 $T_{peak}/C_{peak}$와 $T_{area}/C_{area}$값을 이용하여 다중회귀분석을 수행하였으며 개발된 다중회귀방정식 중에서 가장 단순하고 결정계수가 높은 다중회귀방정식을 선정하였다. 추가로 제작된 간이진단 키트를 이용하여 개발된 다중회귀방정식을 검증한 결과 결정계수가 0.9393으로 우수한 선형성을 나타내었다. 본 연구에서 개발된 다중회귀방정식을 이용하여 더 정확하게 간이진단 키트에서 측정된 살모넬라 균수를 예측하는 것이 가능하였다.

• 대한수의학회지
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• 제58권1호
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• pp.27-31
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• 2018

Canine coronavirus is a single-stranded RNA virus that causes enteritis in dogs of any age. Coronaviral enteritis is seldom definitively diagnosed, since it is usually much less severe than many other types of enteritis and is self-limiting. Conventional diagnostics for the canine coronaviral enteritis such as polymerase chain reaction (PCR), virus isolation, and electron microscopic examination are inappropriate for small animal clinics due to the complicated experimental processes involved. Therefore, a commercially available lateral flow test kit based on chromatographic immunoassay techniques was tested to evaluate its performance as a first-line diagnostic test kit that could be used in clinics. The coronavirus antigen test kit detected canine coronavirus-infected dogs with 93.1% sensitivity and 97.5% specificity. The detection limit of the test kit was between $1.97{\times}10^4/mL$ and $9.85{\times}10^3/mL$ for samples with a 2-fold serial dilution from $1.25{\times}10^6\;TCID_{50}$ ($TCID_{50}$, 50% tissue culture infectious dose). Additionally, the test kit had no cross-reactivity with canine parvovirus, distemper virus, or Escherichia coli. Overall, the commercially available test kit showed good diagnostic performance in a clinical setting, with results similar to those from PCR, confirming their potential for convenient and accurate use in small animal clinics.

• KSBB Journal
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• 제31권1호
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• pp.46-51
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• 2016

Recently paper based diagnostic kits have drawn great interest in the point-of-care testing market (POCT). The paper based detection systems provide inexpensive, rapid and safe analyses for disease markers and/or pathogens. Vitamin C (i.e., ascorbic acid) regulates body's immune system as an antioxidant agent. Humans, however, do not have enough amounts of enzymes involved in the synthesis of vitamin C that it is required to be obtained from their diets (e.g., beverages and/or supplements). Here, we have prepared a paper based kit to detect the concentration of Vitamin C presented in commercially available beverages. The evaluation provides the fast, simple and accurate results for detecting Vitamin C in the prepared paper based kit.

• Toxicological Research
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• 제20권2호
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• pp.101-108
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• 2004

유전자변형작물의 이점과 잠재적 위해에 관한 다른 사회적 인식은 각국에서 다른 반응을 유발시켜왔다 한국을 포함한 많은 국가는 새로운 규제를 제정하기 위해 부심하고 있다 한국은 최근에 3% 이상의 유전자변형작물 혼입을 포함하는 모든 식품에 표시제를 실시하였다. 유전자변형작물 혼입을 신속하고 간편하게 검출하는 방법의 하나는 PCR에 의한 도입 DNA의 증폭이다 이 목적을 위한 많은 PCR kit가 시판되고 있어, 본 연구는 이들 상업화된 유전자변형작물 검출 kit의 성능을 시험하였다. 그 결과 이들 15개 kit 중 6개는 안정성, 재현성 및 검출 한계의 측면에서 제작사 스스로 제시한 요구조건도 충족하지 못하여 이들 kit의 개발 및 생산 단계에서 품질관리에 문제점이 있음을 시사하였다 본 시험은 또한 duplex와 triplex검출 kit가 단일 PCR 반응에서 명백한 검출을 보장할지라도, monoplex 검출 kit의 검출 능력이 가장 높다는 것을 시사하였다. 또한, kit들은 콩과 옥수수 사이에서 다른 검출 한계를 보였다. 본 연구의 결과는 GM 작물의 재배, 국가간 이동, GM 작물을 사용한 식품 생산 과정의 모니터링 뿐만 아니라 GM작물과 관련한 정부의 법규를 준수하기 위한 GM작물의 혼입의 건전한 과학적 추적체계의 개발에 유용할 것이라 사료된다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.264-265
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• 2000

Helicobacter pylori(H. pylori) is the causative agent of chronic gastritis and the single most important factor in peptic ulcer disease, however, the pathogenetic mechanisms underlying H, pylori infection are not well understood. Futhermore, there is a strong association between H. pylori infection and gastric cancer. Various diagnostic methods for detecting H. pylori infection are available. These can be divided into invasive methods, requiring endoscopy, and non-invasive tests, mainly 13C-urea breath tests and serologic detection of antibodies. Rapid urease test is the most recommendable endoscopic test for the diagnosis of H. pylori infection, presently. CLO test kit is the represent of rapid urease test kits. The principles of CLO test kit is that hydrolysis of urea by urease Is detected by a dye indicators showing a color change. Our device is used same principle but we improved the reaction time is more faster and positive color change is more distinctive from the color of the negative specimen. So, this kit is more reliable because it response faster and accuracy.