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http://dx.doi.org/10.4014/jmb.1307.07035

Development of a Novel Immunochromatographic Assay for Rapid Detection of VanA Ligase-Producing Vancomycin-Resistant Enterococci  

Ji, Gil Yong (Department of Pathology Medicine, Chungbuk National University College of Medicine)
Song, Hyung Geun (Department of Pathology Medicine, Chungbuk National University College of Medicine)
Son, Bo Ra (Department of Laboratory Medicine, Chungbuk National University College of Medicine)
Hong, Seung Bok (Department of Clinical Laboratory Science, Chungbuk Health and Science University)
Kim, Jong Wan (Department of Laboratory Medicine, Dankook University College of Medicine)
Shin, Kyeong Seob (Department of Laboratory Medicine, Chungbuk National University College of Medicine)
Publication Information
Journal of Microbiology and Biotechnology / v.24, no.3, 2014 , pp. 427-430 More about this Journal
Abstract
We developed a novel immunochromatographic assay (ICA) (EZ-Step VanA rapid kit; Dinona, Korea) for the detection of VanA ligase from vancomycin-resistant enterococci (VRE). Of eight monoclonal antibodies screened by ELISAs, the VanA ligase ICA constructed with 1H9 plus 3G11 showed the greatest reactivity. The detection limit of the kit was $6.3{\times}10^6$ CFU per test. Of 127 vancomycin-resistant microorganisms, 100 vanA VRE were positive in the VanA ligase ICA, and 27 non-vanA vancomycin-resistant isolates were negative. These results were consistent with those of the PCR analyses. Thus, our ICA is a reliable and easy-to-use immunological assay for detecting VanA-producing VRE in clinical laboratories.
Keywords
Vancomycin resistance; VanA; immunochromatographic assay; rapid detection; Enterococcus;
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