• 대한임상검사과학회지
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• 제41권2호
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• pp.76-82
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• 2009

It would be one of the most important tests that determination of semen in crime scene as a matter of significant evidences. Recently, it has been developed for the identification of semen in forensic specimens which was used simply, easily and reproductively. In this study, Prostate-Specific-Antigen (PSA) Rapid Test kit was evaluated for the forensic identification of semen and compared with one step semen inspection forensic rapid test kit. The sensitivity and specificity of the rapid PSA kit were examined in addition to the stability of PSA. The positive band of rapid PSA kit shown even with 1,000,000-fold diluted semen, which was at least 100 timed higher than qualitative one step semen inspection forensic rapid test kit. PSA was detected in urine from normal male adult, however, it was not detected in urine from young boys and female body fluids. It was shown that PSA was very stable to resist boiling for 20 minutes and the effect of bacteria. In crime scene investigation, rapid PSA kit is expected to help to identify semen easily in the evidences.

• 분석과학
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• 제31권5호
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• pp.201-207
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• 2018

혈액은 범죄현장에서 가장 흔하게 볼 수 있는 체액 중 하나이며 용의자를 확인하고 사건 현장을 재구성하는데 중요한 역할을 한다. 혈액의 혈청학적 식별법은 법과학 분야에서 널리 사용되고 있지만, 오래된 혈흔에 대한 혈청학적 식별법의 비교에 관한 연구는 거의 수행되지 않았다. 본 연구의 목적은 오래된 혈흔의 다양한 식별법을 비교하고 그 유효성에 대한 정보를 제공하는 것이다. 본 연구에서는 Tetramethylbenzidine, $Bluestar^{(R)}$, Leucomalachite Green, Kastle-Meyer의 네 가지 혈액 예비시험과 Fecal Occult Blood (FOB)와 Rapid Stain $Identification^{(TM)}-Blood$ ($RSID^{TM}-Blood$) 두 가지 혈액 확증시험을 사용하여 비교연구를 수행하였다. 혈흔 시료는 최소 7년부터 최대 30년동안 실온에서 거즈에 보관된 부검 혈액을 사용하였다. 실험 결과 네 가지 예비 실험에서는 30년까지 경과된 시료를 포함하여 모든 시료에서 양성반응으로 관찰되었다. 반면에 FOB와 $RSID^{TM}-Blood$는 17년 이상 경과된 일부 시료(1988년부터 2001년)에서 음성이 나타나 오래된 혈흔 시료의 경우 확증시험에서 음성반응이 나타날 수 있음을 확인하였다. 이러한 연구 결과는 오래된 증거물에 대한 혈청학적 감정에서 적절한 혈액 식별법 선정에 도움이 될 수 있을 것으로 기대한다. 더불어 법과학적인 감정분야에서 활용할 때 유용한 배경지식이 되리라 사료된다.

• Parasites, Hosts and Diseases
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• 제59권3호
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• pp.257-263
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• 2021

Human gnathostomiasis is a parasitic disease caused by Gnathostoma nematode infection. A rapid, reliable, and practical immunoassay, named dot immuno-gold filtration assay (DIGFA), was developed to supporting clinical diagnosis of gnathostomiasis. The practical tool detected anti-Gnathostoma-specific IgG4 in human serum using crude extract of third-stage larvae as antigen. The result of the test was shown by anti-human IgG4 monoclonal antibody conjugated colloidal gold. The sensitivity and specificity of the test were both 100% for detection in human sera from patients with gnathostomiasis (13/13) and from healthy negative controls (50/50), respectively. Cross-reactivity with heterogonous serum samples from patients with other helminthiases ranged from 0 (trichinosis, paragonimiasis, clonorchiasis, schistosomiasis, and cysticercosis) to 25.0% (sparganosis), with an average of 6.3% (7/112). Moreover, specific IgG4 antibodies diminished at 6 months after treatment. This study showed that DIGFA for the detection of specific IgG4 in human sera could be a promising tool for the diagnosis of gnathostomiasis and useful for evaluating therapeutic effects.

• 약학회지
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• 제57권5호
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• pp.330-336
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• 2013

"The Act on Medication Treatment of Sexual Impulse of Sex Offenders" known as chemical castration has been effective since July 2011 in Korea. According to the law, monitoring of male sex hormone in urine is enforced to request National Forensic Service more than once a month after injection of medicine designed to reduce sex impulse. We established a rapid and sensitive method for the monitoring of testosterone (T) and epitestosterone (E) in human urine by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Three mL of urine was pretreated by solid-phase extraction for purification and performed enzymatic hydrolysis. The pretreated samples were extracted twice with 2 ml of ethyl acetate and n-hexane (2 : 3). The separation was applied on Thermo Hypersil GOLD C18 column ($1.9{\mu}m$, $100{\times}2.1mm$). A gradient elution of methanol and water of 0.1% formic acid were used as mobile phase and the retention time was less than 10 min. LC-MS/MS system coupled with an electrospray ionization source was performed in multiple reaction monitoring mode. The transitions of the analytes executed as following: m/z $289{\rightarrow}97$, 109 for T and E, m/z $292{\rightarrow}109$ for $T-d_3$ and $E-d_3$ as internal standards. The validation results of the method were satisfactory. The limits of detection were 0.05 ng/ml and the limits of quantification were 0.1 ng/ml. This method was successfully applied to real human urine sample. The developed method will be useful for monitoring T/E ratio in urine of sex offenders.

• 약학회지
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• 제55권2호
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• pp.106-115
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• 2011

Systematic toxicological analysis (STA) means the process for general unknown screening of drugs and toxic compounds in biological fluids. In order to establish STA, in previous study we investigated pattern of drugs & poisons in autopsy cases during 2007~2009 in Korea, and finally selected 62 drugs as target drugs for STA. In this study, rapid and simple drug identification and quantitative analytical program by gas chromatography-mass spectrometry(GC-MS) was developed. The in-house program, "DrugMan", consisted of modified chemstation data analysis menu and newly developed macro modules. Total 55 drugs among 62 target drugs were applied to this program, they were 14 antidepressants, 8 anti-histamines, 5 sedatives/hypnotics, 5 narcotic analgesics, 3 antipsychotic drugs, and etc. For calibration curves, fifty five drugs were divided into four groups of range considering their therapeutic or toxic concentrations in blood specimen, i.e. 0.05~1 mg/l, 0.1~1 mg/l, 0.1~5 mg/l or 0.5~10 mg/l. Standards spiked bloods were extracted by solid-phase extraction (SPE) with trimipramine-D3 as internal standard. Parameters such as retention times, 3 mass fragment ions, and calibration curves for each drug were registered to DrugMan. A series of identification, semi quantitation of target drugs and reporting the results were performed automatically. Calibration curves for most drugs were linear with correlation coefficients exceeding 0.98. Sensitivity rate of DrugMan was 0.90 (90%) for 55 drugs at the level of 0.5 mg/l. For standard spiked bloods at the level of 0.5 mg/l for 29 drugs, semi quantitative concentrations were ranged 0.36~0.64 mg/l by DrugMan. If more drugs are registered to database in DrugMan in further study, it will be useful tools for STA in forensic toxicology.

• 분석과학
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• 제26권5호
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• pp.340-351
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• 2013

The chemical element and structural characterization of different types of cements and its brick stones are been investigated under forensic aspects using X-ray florescence (XRF) and X-ray diffraction (XRD) spectrometer. The XRF provides rapid compositional data for controlling almost all stages of raw materials, clinker and cement. The decisive advantage of XRD methods is based on the unique character of the diffraction patterns of crystalline substances, the ability to distinguish between elements and their oxides, and the possibility to identify chemical compounds, polymeric forms, and mixed crystals by non-destructive examination. Therefore, combination of these examinations is useful and able to apply for the forensic analysis in comparison of cements and brick stones. There are more study remained to determine the viability of method for forensic analysis of brick stones and the limits of the discrimination that can be achieved.

• 한국안전학회지
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• 제34권5호
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• pp.55-62
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• 2019

In this paper, we introduce a case of a fire accident during parking of a large truck that is repeatedly occurring. The shape and location of the combustion and electrical singularity commonly found in other vehicle fire accidents could limit the starter motor as the ignition section. In addition, it was possible to confirm the electrical melting singularity that could act as a cause of ignition between the start motor B terminal and the start motor enclosure. By combining the above investigations and investigations, it was possible to estimate the electric fire expressed from insulation breaking of the starter motor B terminal, and by using the renewable starter motor comparison product mounted on the fire vehicle, an experiment was performed to reproduce the ignition process from the starter motor under specific conditions. So. It is hoped that this will raise awareness about vehicle fires, which can lead to large fires or casualties, share the risks of using starter motors for regeneration, and help in the rapid and accurate investigation of similar vehicle fires in the future.

• 대한수사과학회지
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• 제1권1호
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• pp.45-53
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• 2006

The purposes of fire investigation are to identify and analyze the causes of fire and, using the results in promoting fire fighting policies, ultimately to prevent fires caused by similar causes and to protect people's life and properties from fire. However, although the patterns of fire are getting more diverse and complicated with the rapid development of industry, complicated with the rapid development of industry, fire fighting organizations are continuing traditional superficial fire investigation without operating a fire investigation department for the reason of insufficient manpower, equipment and budget, losing public confidence. Thus, the present study purposed to propose plans to improve the technicality of fire investigation and win public confidence in fire investigation. First, the technicality of fire investigation should be improved by operating a department and personnel exclusively for fire investigation. Second, fire investigation should be more scientific and precise using high tech fire investigation equipment. Lastly, responsible fire fighting administration should be realized through statistical analysis of the causes of fire and computerization. The results of this study are expected to contribute to the safety and security of our society.

• 한국정보통신학회논문지
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• 제18권7호
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• pp.1495-1504
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• 2014

인터넷 뱅킹과 같은 부가서비스, 채팅 등과 같은 대화형 서비스를 이용하는 정보화 사회가 정착되었고, 더욱이 스마트기기를 이용한 서비스 사용이 급속하게 발전함에 따라 신규 보안기술 분야로 디지털 포렌식에 관한 연구가 활발히 진행되고 있다. 본 논문에서는 디지털 포렌식에 관한 기존의 연구를 체계적으로 분석하고 앞으로 정부의 ICT R&D 중장기 전략과 ICT 패러다임 변화를 반영하여 첨단 IT기술과 우리나라 법체제를 융합하는 체계적인 디지털 포렌식 표준정립을 위한 기술-정책적 통합프로세스 프레임워크를 제안한다.

• 한국안전학회지
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• 제26권3호
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• pp.23-28
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• 2011

This paper studied combustion characteristics of the building materials such as polyurethane-foam, sponge type sound-absorbing materials and styrofoam. To estimate of the combustion characteristics, we carried out combustion experiment of the building materials. And then to evaluate the suitability of the building materials, we measured heat release rate(HRR) and smoke density(Ds) of polyurethane-foam, sponge type sound-absorbing materials and styrofoam using by a cone-calorimeter. From the combustion experimental results, the general type sound-absorbing materials (GSAM) and styrofoam were rapid burned simultaneously with ignition and the incombustibility type sound-absorbing materials(ISAM) and polyurethane-foam had all gone out simultaneously with ignition. Measured results of HRR and Ds were not satisfied KS F ISO 5660-1 and IMO FTP Code, from the results, the polyurethane-foam, the sponge type sound-absorbing material and the styrofoam were ill-suited for using building interior materials.