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Development of Rapid and Simple Drug Identification and Semi Quantitative Analytical Program by Gas Chromatography-Mass Spectrometry  

Kim, Eun-Mi (Drug & Toxicology Div., National Forensic Service)
Han, Eun-Young (Drug & Toxicology Div., National Forensic Service)
Hong, Hyo-Jeong (Drug & Toxicology Div., National Forensic Service)
Jeong, Su-Jin (Drug & Toxicology Div., National Forensic Service)
Choe, Sang-Gil (Narcotic Analysis Div., National Forensic Service)
Rhee, Jong-Sook (Drug & Toxicology Div., National Forensic Service)
Jung, Jin-Mi (Drug & Toxicology Div., National Forensic Service)
Yeom, Hye-Sun (Drug & Toxicology Div., National Forensic Service)
Lee, Han-Sun (Drug & Toxicology Div., National Forensic Service)
Lee, Sang-Ki (Drug & Toxicology Div., National Forensic Service)
Publication Information
YAKHAK HOEJI / v.55, no.2, 2011 , pp. 106-115 More about this Journal
Abstract
Systematic toxicological analysis (STA) means the process for general unknown screening of drugs and toxic compounds in biological fluids. In order to establish STA, in previous study we investigated pattern of drugs & poisons in autopsy cases during 2007~2009 in Korea, and finally selected 62 drugs as target drugs for STA. In this study, rapid and simple drug identification and quantitative analytical program by gas chromatography-mass spectrometry(GC-MS) was developed. The in-house program, "DrugMan", consisted of modified chemstation data analysis menu and newly developed macro modules. Total 55 drugs among 62 target drugs were applied to this program, they were 14 antidepressants, 8 anti-histamines, 5 sedatives/hypnotics, 5 narcotic analgesics, 3 antipsychotic drugs, and etc. For calibration curves, fifty five drugs were divided into four groups of range considering their therapeutic or toxic concentrations in blood specimen, i.e. 0.05~1 mg/l, 0.1~1 mg/l, 0.1~5 mg/l or 0.5~10 mg/l. Standards spiked bloods were extracted by solid-phase extraction (SPE) with trimipramine-D3 as internal standard. Parameters such as retention times, 3 mass fragment ions, and calibration curves for each drug were registered to DrugMan. A series of identification, semi quantitation of target drugs and reporting the results were performed automatically. Calibration curves for most drugs were linear with correlation coefficients exceeding 0.98. Sensitivity rate of DrugMan was 0.90 (90%) for 55 drugs at the level of 0.5 mg/l. For standard spiked bloods at the level of 0.5 mg/l for 29 drugs, semi quantitative concentrations were ranged 0.36~0.64 mg/l by DrugMan. If more drugs are registered to database in DrugMan in further study, it will be useful tools for STA in forensic toxicology.
Keywords
systematic toxicological analysis (STA); GC-MS; DrugMan;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Ishida, T., Kudo, K., Naka, S., Toubou, K., Noguchi, T. and Ikeda, N. : Rapid diagnosis of drug intoxication using novel $NAGINATA^{TM}$ gas chromatography/mass spectrometry software. Rapid Communications in Mass Spectrometry 21, 3129 (2007).   DOI   ScienceOn
2 Kudo, K., Ishida, T., Hikiji, W., Hayashida, H., Uekusa, K., Usumoto, Y., Tsuji, A. and Ikeda, N. : Construction of calibration-locking database for rapid and reliable drug screening by gas chromatography-mass spectrometry. Forensic. Toxicol. 27, 21 (2009).   DOI   ScienceOn
3 Kudo, K., Ishida, T., Hikiji, W., Usumoto, Y., Umehara, T., Nagamatsu, K., Tsuji, A. and Ikeda, N. : Pattern of poisoning in Japan: selection of drugs and poisons for systematic toxicological analysis. Forensic. Toxicol. 28, 25 (2010).   DOI   ScienceOn
4 김은미, 이주선, 최혜영, 임미애, 정희선 : 고상추출법 및 NCIGC-MS를 이용한 소변 중 15종 벤조디아제핀계 약물 동시분석. 한국법과학회지 8, 24 (2007).
5 Peters, F. T. : Analytical Method Development and Validation, FBI Laboratory Symposium on Forensic Toxicology, Washington D.C., August 29-30 (2004).
6 김은미, 김지현, 홍효정, 정수진, 인상환, 이종숙, 정진미, 이한선, 이상기 : 최근 3년간 국내 변사체 중 약독물 검출 유형(2007-2009)-약독물 검출 시스템 확립을 위한 목표 약물의 선별-. 약학회지 54, 341 (2010).
7 최혜영, 이주선, 최상길, 김은미, 김재균, 김영운, 임미애, 정희선 : 음주운전자 275명 혈액 중 마약류 및 남용약물의 분석. 약학회지 52, 137 (2008).
8 인상환, 김지현, 홍효정, 이정아, 정진일, 민승식, 하정민, 최화경, 정희선 : LC/MS 및 direct MS에 의한 약물 분석 및 Library 구축(II), 27종의 수면진정제류. 국립과학수사연구소연보 40, 334 (2008).
9 Baselt, R. C. : Disposition of Toxic Drugs and Chemicals in Man, 8th edi, Biomedical Publications (2008).
10 Molina, D. K. : Handbook of Forensic Toxicology for Medical Examiners, CRC Press (2010).
11 Moffat, A. C., Osselton, M. D. and Widdop, B. : Clarke's Analysis of Drugs and Poisons in pharmaceuticals, body fluids and postmortem material, 3rd edi, Pharmaceutical Press (2004).
12 Reference blood level list of therapeutic and toxic substances, http://www.tiaft.org.
13 Polettini, A. : Applications of LC-MS in Toxicology, Pharmaceutical Press (2006).
14 Trullols, E., Ruisanchez, I. and Rius, F. X. : Validation of qualitative analytical methods. Trends in Analytical Chemistry 23, 137 (2004).   DOI   ScienceOn
15 Kadokami, K., Tanada, K., Taneda, K. and Nakagawa, K. : Novel gas chromatography-mass spectrometry database for automatic identification and quantification of micropollutants. Journal of Chromatography A 1089, 219 (2005).   DOI