Browse > Article
http://dx.doi.org/10.5806/AST.2018.31.5.201

Comparative study of serological detection methods on old bloodstain samples  

Lee, Minji (Forensic DNA division, National Forensic Service)
Ahn, Eu-Ree (Forensic DNA division, National Forensic Service)
Kim, Da-Hye (Forensic DNA division, National Forensic Service)
Shin, Heejin (Forensic DNA division, National Forensic Service)
Jung, Ju Yeon (Forensic DNA division, National Forensic Service)
Lee, Sung-Jin (Forensic DNA division, National Forensic Service)
Chun, Byung-Won (Forensic DNA division, National Forensic Service)
Publication Information
Analytical Science and Technology / v.31, no.5, 2018 , pp. 201-207 More about this Journal
Abstract
Blood is a commonly found body fluid at crime scenes, and plays an important role in identifying suspects and in the reconstruction of crime scenes. Although serological detection of blood has been widely used in the field of forensic science, research on the detection of old bloodstains is scarce. This work aimed to compare various methods for the detection of old bloodstains and validate the reliability of their results. Four presumptive tests-Tetramethylbenzidine, $Bluestar^{(R)}$, Leucomalachite Green, Kastle-Meyer tests-and two confirmatory tests-Fecal Occult Blood (FOB) and Rapid Stain $Identification^{(TM)}-Blood$ ($RSID^{TM}-Blood$) tests-were compared. Bloodstain samples from post-mortem cases were collected on gauzes and then stored at room temperature for periods from 7 to 30 years. All the presumptive tests were positive, even for the 30-year-old sample. However, FOB and $RSID^{TM}-Blood$ provided false negative results for some samples stored for 17 years or more (1988 to 2001). The results indicate that FOB and $RSID^{TM}-Blood$ are not reliable for the detection of old bloodstains. These findings can be useful in the selection of an appropriate detection method for serological testing of old bloodstains. In addition, the information will be useful background knowledge when applied in the field of forensic practice.
Keywords
old bloodstain; serological detection; presumptive test; confirmatory test;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 K. Virkler and I. K. Lednev, Forensic Sci. Int., 188, 1-17 (2009).   DOI
2 H. Y. Lee, K.-J. Shin, and W. I. Yang, Korean J. Leg Med., 33, 84-88 (2009).
3 J. H. An, K-J. Shin, and W. I. Yang. BMB Rep., 45(10), 545-553 (2012).   DOI
4 L. Kobilinsky, 'Forensic Chemistry Handbook', John Wiley&Sons, New Jersey, 2011.
5 한면수, 과학수사와 증거재판에서 DNA프로필의 역할, 경찰학연구 제4호, 경찰대학, 173-269 (2003).
6 R. Li, 'Forensic Biology', 2nd Ed., Boca Raton, 2008.
7 J. Siegel and P. Saukko, 'Encyclopedia of Forensic Sciences', 2nd ed., London, 2012.
8 R. Saferstein, 'Forensic Science Handbook', Vol. 2, New Jersey, 1988.
9 L. J. Blum, P. Esperanca, and S. Rocquefelte., Can. Soc. Forensic Sci. J., 39. 81-100 (2006).   DOI
10 J. L. Webb, J. I. Creamer, and T. I. Quichenden., Luminescence, 21(4), 214-220 (2006).   DOI
11 M. vandewoestyne and T. lepez., J. Forensic Sci., 60(3), 707-711 (2015).   DOI
12 S. S. Tobe, N. Watson, and N. N. Daeid, J. Forensic Sci., 52(1), 102-109 (2007).   DOI
13 E. Johnston, C. E. Ames, K. E. Dagnall, and J. Foster., J. Forensic Sci., 53(3), 687-689 (2008).   DOI
14 S. K. Lim, K. W. Park, and S. K. Choi., Anal. Sci. & Technol., 17, 211-216 (2004).
15 I. horjan, L. Barbaric, and G. Mrsic., J. Forensic Leg Med., 38, 101-105 (2016).   DOI
16 S. Turrina and G. Filippini., Forensic Sci. Int. Genet. Suppl. Series, 1, 74-75 (2008).   DOI
17 H. C. Lee, T. Palmbach, and M. T. Miller. 'Henry Lee's Crime scene handbook. '1st Ed., London, 2001.
18 김택수, 황문규, 김슬기, '중요강력범죄 장기미제사건 수사를 위한 효율적 제도 연구', 경찰청 용역연구보고서, 2013.
19 Claudia Gomes, Cesar Lopez-Matayoshi, Sara Palome-Diez, Ana Maria Lopez-Parra, Pedro Cuesta-Alvaro, Carlos Baeza-Richer, Juan F. Gibaja, and Eduardo Arroyo-Pardo, Forensic Sci. Int. Genet. Suppl. Series, 6, e546-e548 (2017).   DOI
20 Blanchard, D., Transfus. Med. Rev., 4(3), 170-186 (1990).   DOI
21 S. Alters, 'Biology: Understanding Life', 3rd Ed., Jones & Bartlett Learning, Massachusetts, (2000).