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Diversity of the Streptococcal Strains Isolated from Diseased Olive Flounder (Paralichthys olivaceus) (넙치 (Paralichthys olivaceus) 병어에서 분리된 연쇄상구균의 다양성)

  • KIM Jong-Hun;KIM Eunheui
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.36 no.6
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    • pp.654-660
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    • 2003
  • To evaluate the biological diversity of fish pathogenic streptococci, 35 strains isolated from diseased olive flounder (Paralichtys olivaceus), were analyzed using a random amplified polymorphic DNA (RAPD) technique with the oligonucleotide commercial primer 6 (Amersham Biosciences). Api 20 Strep test, drug resistance and artificial infection were carried out for further characterization of the isolates. RAPD fingerprints showed similar pattern in 25 strains (about $71.4\%$ of 35 isolates) and these strains were designed as RA group 1. Similarities greater than $44\%$ were obtained when the Dice coefficient was applied among the isolates of RA 1. On the other hand, the reference Streptococcus iniae showed a similar RAPD profile to the isolates with similarity levels of $40-93.3\%.$ Rh I was suggested to be the dominant group isolated from olive flounder suffering from streptococcosis. However, the isolates of Rh 1 group were not classified into the same species by the Api 20 Strep identification system. There was no peculiarity in drug resistance patterns of Rh I group isolates against 7 antibacterial agents. However, only 3 of 25 isolates $(0.12\%)$ showed oxytetracycline (OTC) resistance and OTC might be a useful chemotherapeutic agent in controlling the streptococcosis by strains of RA I group in olive flounder. Fish injected intraperitoneally with $10^5$ CFU of an isolate of Rh I and RA III group showed $60\%\;and\;50\%$ accumulative mortality for 20 days, respectively ($20\%$ in control or Rh II). However luther comparative studies about differences in virulence between isolates are needed.

Free-Form Surface Reconstruction Method from Second-Derivative Data (형상이차미분을 이용한 자유곡면 형상복원법)

  • Kim, Byoung Chang;Kim, DaeWook;Kim, GeonHee
    • Korean Journal of Optics and Photonics
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    • v.25 no.5
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    • pp.273-278
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    • 2014
  • We present an algorithm for surface reconstruction from the second-derivative data for free-form aspherics, which uses a subaperture scanning system that measures the local surface profile and determines the three second-derivative values at those local sampling points across the free-form surface. The three second-derivative data were integrated to get a map of x- and y-slopes, which went through a second Southwell integration step to reconstruct the surface profile. A synthetic free-form surface 200 mm in diameter was simulated. The simulation results show that the reconstruction error is 19 nm RMS residual difference. Finally, the sensitivity to noise is diagnosed for second-derivative Gaussian random noise with a signal to noise ratio (SNR) of 16, the simulation results proving that the suggested method is robust to noise.

Etching characteristic of SBT thin film by using Ar/$CHF_3$ Plasma (Ar/$CHF_3$ 플라즈마를 이용한 SBT 박막에 대한 식각특성 연구)

  • 서정우;이원재;유병곤;장의구;김창일
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 1999.11a
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    • pp.41-43
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    • 1999
  • Among the feffoelectric thin films that have been widely investigated for ferroelectric random access memory (FRAM) applications, SrBi$_2$Ta$_2$$O_{9}$ thin film is appropriate to memory capacitor materials for its excellent fatigue endurance. However, very few studies on etch properties of SBT thin film have been reported although dry etching is an area that demands a great deal of attention in the very large scale integrations. In this study, the a SrBi$_2$Ta$_2$$O_{9}$ thin films were etched by using magnetically enhanced inductively coupled Ar/CHF$_3$ plasma. Etch properties, such as etch rate, selectivity, and etched profile, were measured according to gas mixing ratio of CHF$_3$(Ar$_{7}$+CHF$_3$) and the other process conditions were fixed at RF power of 600 W, dc bias voltage of 150 V, chamber pressure of 10 mTorr. Maximum etch rate of SBT thin films was 1750 A77in, under CHF$_3$(Ar+CHF$_3$) of 0.1. The selectivities of SBT to Pt and PR were 1.35 and 0.94 respectively. The chemical reaction of etched surface were investigated by X-ray photoelectron spectroscopy (XPS) analysis. The Sr and Ta atoms of SBT film react with fluorine and then Sr-F and Ta-F were removed by the physical sputtering of Ar ion. The surface of etched SBT film with CHF$_3$(Ar+CHF$_3$) of 0.1 was analyzed by secondary ion mass spectrometer (SIMS). Scanning electron microscopy (SEM) was used for examination of etched profile of SBT film under CHF$_3$(Ar+CHF$_3$) of 0.1 was about 85˚.85˚.˚.

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A Response Prediction Model for the Vortex-Induced Vibration of Marine Risers in Sheared Flow (전단류중 Marine Riser의 와류유기 진동 예측모델에 관한 연구)

  • Yong-Yun,Nam;Tae-Young,Chung
    • Bulletin of the Society of Naval Architects of Korea
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    • v.26 no.2
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    • pp.64-72
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    • 1989
  • A response prediction model is introduced for the non-lockin vibration of a marine riser in sheared flow, where the riser is modelled as linearly varying tensioned-beam. This prediction model is based on the Green's function approach and random vibration theory. This model, of course, can treat general beams having slowly varying spatial system parameters. According to the predicted result of a marine riser by the prediction model proposed in this paper, the dynamic behavior of a marine riser has the mixed characteristics of finite and infinite boundary behavior. Furthermore the velocity response distribution along the riser length is much similar with the sheared flow profile. The predicted response result of a marine riser having linearly varying tension was also compared to that of constant mean tensioned-beam model. It was found that the constant mean tensioned-beam case gives over-estimated responses at the top side of the riser.

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A combination method to generate fluctuating boundary conditions for large eddy simulation

  • Wang, Dayang;Yu, X.J.;Zhou, Y.;Tse, K.T.
    • Wind and Structures
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    • v.20 no.4
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    • pp.579-607
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    • 2015
  • A Combination Random Flow Generation (CRFG) technique for obtaining the fluctuating inflow boundary conditions for Large Eddy Simulation (LES) is proposed. The CRFG technique was developed by combining the typical RFG technique with a novel calculation of k and ${\varepsilon}$ to estimate the length- and time-scales (l, ${\tau}$) of the target fluctuating turbulence field used as the inflow boundary conditions. Through comparatively analyzing the CRFG technique and other existing numerical/experimental results, the CRFG technique was verified for the generation of turbulent wind velocity fields with prescribed turbulent statistics. Using the turbulent velocity fluctuations generated by the CRFG technique, a series of LESs were conducted to investigate the wind flow around S-, R-, L- and U-shaped building models. As the pressures of the models were also measured in wind tunnel tests, the validity of the LES, and the effectiveness of the inflow boundary generated by the CRFG techniques were evaluated through comparing the simulation results to the wind tunnel measurements. The comparison showed that the LES accurately and reliably simulates the wind-induced pressure distributions on the building surfaces, which indirectly validates the CRFG technique in generating realistic fluctuating wind velocities for use in the LES. In addition to the pressure distribution, the LES results were investigated in terms of wind velocity profiles around the building models to reveal the wind flow dynamics around bluff bodies. The LES results quantitatively showed the decay of the bluff body influence when the flow moves away from the building model.

Analysis on the Characteristics of Government-dominant and Non-governmental Food Bank Programs from the Users' Perspective (정부주도형 및 민간주도형 푸드뱅크(Food Bank) 사업의 이용자 실태 및 이용특성 분석)

  • Kang Hye-Seune;Hong Min-Ah;Yang Il-Sun;Jo Mina;Kim Chul-Jae
    • Korean Journal of Community Nutrition
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    • v.10 no.2
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    • pp.224-233
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    • 2005
  • The objectives of this study were to (a) examine the users' characteristics of both government-dominant and non-governmental food bank programs, (b) understand the supporting quality of the food bank program, and (c) analyse the effectiveness of the programs from the users' perspectives. The random samples of 120 food bank operators were selected by proportionate stratified random sampling method and from each food bank, 3 users were selected for the survey. A total of 205 users were participated in this survey. The main results of this study were as follows; Average profile of food bank users were 58 year old, female, livelihood protectee ($56.6\%$). Food assistance frequency were mostly once a week ($34.1\%$), and everyday ($22.4\%$). The survey also showed that non-governmental food banks delivered the food directly to the $42.7\%$ of users, otherwise the users of government-dominant ones went to the food bank to receive the foods. Most of the donated foods were cooked food ($50.0\%$), and the users also wanted to receive that kinds of food. Saving food expenses ($55.6\%$) and overcoming hunger ($16.6\%$) were found as the effectiveness to the users.

Dry Etching of NiFe, NiFeCo, and Ta in Cl2/Ar Inductively Coupled Plasma (Cl2/Ar 유도 결합 플라즈마를 이용한 NiFe, NiFeCo, Ta의 건식식각)

  • Ra, Hyun-Wook;Park, HyungJo;Kim, Ki Ju;Kim, Wan-Young;Hahn, Yoon-Bong
    • Korean Chemical Engineering Research
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    • v.43 no.1
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    • pp.76-79
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    • 2005
  • Dry etching of NiFe, NiFeCo, and Ta for magnetic random access memory (MRAM) by inductively coupled plasmas (ICPs) of $Cl_2/Ar$ has been carried out. NiFe and NiFeCo showed maximum etch rates at a particular ICP source power, but the etch rate of Ta increased with the ICP source power. The etch rates of the magnetic thin films increased with the RF chuck power, but decreased with the operating pressure and the $Cl_2$ concentration. To avoid a corrosion problem by chlorine, the etched samples were rinsed with de-ionized water for 5 minutes after etching. The etch profile showed a clean and smooth surface at 50% $Cl_2$ concentration.

Reliability Design Analysis for Underwater Buriend PBA Based on PoF (고장물리 기반 수중 매설형 PBA에 대한 신뢰성 설계 연구)

  • Kim, Ji-Young;Lee, Ki-Won;Yoon, Hong-Woo;Lee, Seung-Jin;Heo, Jun-Ki;Kwon, Hyeong-Ahn
    • Journal of Applied Reliability
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    • v.17 no.4
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    • pp.280-288
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    • 2017
  • Purpose: PBA buried in underwater requires high reliability because of its mission critical characteristic and harsh operational environment during its life cycle. Therefore, various reliability improvement activities are necessary. The defect on PBA manufacturing process have been studied, as a result, many activities and standards have been presented. However, there are less studies regarding failure pattern on physical features based on design. In this paper, we studied a possible failure patten based on physical features that is related with manufacturing process of PBA. And reliability improvement design based on PoF (Physical of Failure) were intruduced in this paper. Methods: A reliability prediction simulation were performed on the components A and B of the H system using Sherlock Software which is a PoF commercial tool from DFR solution. Solder fatigue and PTH fatigue analysis based on thermal cycling profiles and random vibration was analyzed on three earthquake response spectrum. Result: It was validated that life time and reliability improvement design through solder fatigue and PTH fatigue analysis in case of component. For compoenet B, random vibration fatigue was additionally analyzed and validated reliability for earthquakes profile. Conclusion: In design stage prior to manufacturing, PoF can be analyzed, and it is possible to make a reliability improvement/validated design using design data. This study can be applied in every design step and contribute to make more stable development product.

A computer based simulation model for the fatigue damage assessment of deep water marine riser

  • Pallana, Chirag A.;Sharma, Rajiv
    • Ocean Systems Engineering
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    • v.12 no.1
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    • pp.87-142
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    • 2022
  • An analysis for the computation of Fatigue Damage Index (FDI) under the effects of the various combination of the ocean loads like random waves, current, platform motion and VIV (Vortex Induced Vibration) for a certain design water depth is a critically important part of the analysis and design of the marine riser platform integrated system. Herein, a 'Computer Simulation Model (CSM)' is developed to combine the advantages of the frequency domain and time domain. A case study considering a steel catenary riser operating in 1000 m water depth has been conducted with semi-submersible. The riser is subjected to extreme environmental conditions and static and dynamic response analyses are performed and the Response Amplitude Operators (RAOs) of the offshore platform are computed with the frequency domain solution. Later the frequency domain results are integrated with time domain analysis system for the dynamic analysis in time domain. After that an extensive post processing is done to compute the FDI of the marine riser. In the present paper importance is given to the nature of the current profile and the VIV. At the end we have reported the detail results of the FDI comparison with VIV and without VIV under the linear current velocity and the FDI comparison with linear and power law current velocity with and without VIV. We have also reported the design recommendations for the marine riser in the regions where the higher fatigue damage is observed and the proposed CSM is implemented in industrially used standard soft solution systems (i.e., OrcaFlex*TM and Ansys AQWA**TM), Ms-Excel***TM, and C++ programming language using its object oriented features.

Development of a Returnable Folding Plastic Box RFID Module for Agricultural Logistics using Energy Harvesting Technology (에너지 하베스팅 기술을 활용한 농산물 물류용 리턴어블 접이식 플라스틱 상자 RFID 모듈 개발)

  • Jong-Min Park;Hyun-Mo Jung
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.29 no.3
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    • pp.223-228
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    • 2023
  • Sustainable energy supplies without the recharging and replacement of the charge storage device have become increasingly important. Among various energy harvesters, the triboelectric nanogenerator (TENG) has attracted considerable attention due to its high instantaneous output power, broad selection of available materials, eco-friendly and inexpensive fabrication process, and various working modes customized for target applications. In this study, the amount of voltage and current generated was measured by applying the PSD profile random vibration test of the electronic vibration tester and ISTA 3A according to the time of Anodized Aluminum Oxide (AAO) pore widening of the manufactured TENG device Teflon and AAO. The discharge and charging tests of the integrated module during the random simulated transport environment and the recognition distance of RFID were measured while agricultural products (onion) were loaded into the returnable folding plastic box. As a result, it was found that AAO alumina etching processing time to maximize TENG performance was optimal at 31 min in terms of voltage and current generation, and the integrated module applied with the TENG module showed a charging effect even during the continuous use of RFID, so the voltage was kept constant without discharge. In addition, the RFID recognition distance of the integrated module was measured as a maximum of 1.4 m. Therefore, it was found that the surface condition of AAO, a TENG element, has a great influence on the power generation of the integrated module, and due to the characteristics of TENG, the power generation increases as the surface dries, so it is judged that the power generation can be increased if the surface drying treatment (ozone treatment, etc.) of AAO is applied in the future.