• 대한한방부인과학회지
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• 제18권3호
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• pp.77-91
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• 2005

Purpose : Trichosanthis Radix is traditional medical herb which has been shown to inhibit tumor cell proliferation. In this study, the effects of Trichosanthis Radix extract were investigated on inducing growth inhibition and apoptosis of human uterine cervical carcinoma cells. Methods : Human uterine cervical carcinoma cells line, ME-180, was used for the study. The cells were treated with varying concentrations of Trichosanthis Radix extract. Cell growth and inhibitory rate were measured by MTT assay. Apoptosis induction was detected by fluorescence microscopy, DNA ladder formation and flow cytometry. Results : Trichosanthis Radix extract inhibited the growth of human uterine cervical carcinoma cells in a dose-dependent manner. It induced ME-180 cells to undergo apoptosis including fragmented nuclei and nucleosome-sized DNA fragmentation. Flow cytometric analysis showed the increasing rate of apoptotic cells by Trichosanthis Radix extract. Reduction of mitochondrial membrane potential and increase in caspase-3 activity and were found in ME-180 cells treated with Trichosanthis Radix extract. Conclusion : Our data suggest that Trichosanthis Radix extract inhibit the growth and proliferation of ME-180 cells by apoptotic induction and facilitates its activity via caspase-3 activation initiated by depolarization of mitochondria.

• 대한한방소아과학회지
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• 제16권1호
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• pp.81-103
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• 2002

This present study was carried out to investigate the effect of Saengjingamrotang and Saengjingamrotang plus Radix Trichosanthis in streptozotocin induced hyperglycemic rats. Body weigh, serum levels of glucose, insulin, total cholesterol, triglyceride and urine levels of volume, glucose, protein were measured in streptozotocin induced hyperglycemic rats orally receiving extracts of Sanengjingamrotang and Saengjingamrotang plus Radix Trichosanthis for 4 days. The results were as follows: 1. The change of body weight, the medication group of Saengjingamrotang plus Radix Trichosanthis concentration has the notable increase, the medication group of Saengjingamrotang concentration has no notable change, as compared to control group. 2. The change of the content on serum glucose, the medication group of Saengjingamrotang conctntration have notable decrease, as compared to control group. 3. The change of the content on serum insulin, the medication group of Saengjingamrotang plus Radix Trichosanthis concentration has the notable increase, the medication group of Saengjingamrotang concentration has no notable change, as compared to control group. 4. The change of the content on serum total cholesterol, the medication group of saengjingamrotang concentration and the medication group of Saengjingamrotang plus Radix Trichosanthis concentration have notable decrease, as compared to control group. 5. The change of the content on serum triglyceride, the medication group of Saengjingamrotang concentration and the medication group of Saengjingamrotang plus Radix Trichosanthis Concentration have notable decrease, as compared to control group. 6. The change of urine volume, the medication group of Saengjingamrotang plus Radix Trichosanthis concentration has the notable decrease, the medication group of Saengjingamrotang concentration has no notable change, as compared to control group. 7. The change of the content on urine glucose, the medication group of Saengjingamrotang concentration and the medication group of Saengjingamrotang plus Radix Trichosanthis concentration have notable decrease, as compared to control group. 8. The Change of the content of serum protein, the medication group of Saengjingamrotang concentration and the medication group of Saengjingamrotang plus Radix Trichosanthis concentration have notable decrease, s compared to control group. 9. The change of body weight, serum insulin and urine volume, the medication group of saenfjingamrotang plus Radix Trichosanthis concentration has more notable change than the medication group of Saengjingamrotang concentration, as compared to control group. According to above mentioned results, Saengjingamrotang and Saenfjingamrotang plus Radix Trichosanthis have the effect of decreased blood sugar, serum lipid levels and urine volume, protein, glucose in streptozotocin induced hyperglycemic rats, and so they were expected to be appled to the treatment of diabetes mellitus.

• 한방안이비인후피부과학회지
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• 제14권1호
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• pp.209-225
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• 2001

Recently many efforts were focused to understand the mechanical insights of melanogenesis to develop the agents for hyper-pigmentation and hypo-pigmentation. In the melanin biosynthetic pathway, tyrosinase is the rate limiting enzyme, and ${\alpha}$-melanocyte stimulating hormone(MSH) or cAMP-elevating agents stimulate melanogenesis and enhance the melanin synthesis and the tyrosinase activity. The author has analyzed the effects of Radix Trichosanthis on the basal melanogenic activities of B16/F10 mouse melanoma cells, and on the ${\alpha}$-MSH or forskolin-induced melanogenesis. Radix Trichosanthis alone markedly suppressed melanin content and tyrosinase activity in a dose-dependent manner. Pretreatment of the cells with Radix Trichosanthis also suppressed the increase of ${\alpha}$-MSH (10 nM) or forskolin (20${\mu}M$)-induced melanin content and tyrosinase activity. The decrease in the tyrosinase activity was paralled by a decrease in the abundance of tyrosinase protein and tyrosinase promoter activity. Pretreatment of the cells with Radix Trichosanthis also inhibited the increase of forskolin($20{\mu}M$) induced the amount of tyrosinase protein and tyrosinase promoter activity. The results of DOPA staining revealed that pretreatment of the cells with Radix Trichosanthis showed less intensity than B16 melanoma cells stimulated with ${\alpha}$- MSH or forskolin. These results suggest that Radix Trichosanthis inhibits melanogenesis and abrogates ${\alpha}-MSH and cAMP-induced melanogenesis in B16 melanoma cells.

• 대한한의학회지
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• 제23권4호
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• pp.45-54
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• 2002

Objectives: In order to investigate the relationship of Radix Trichosanthis components and the melanin synthesis, the author has analyzed the cell viability and tyrosinase activity, melanin content and morphologic changes in n-hexane, EtOAc, n-BuOH, and H2O fraction. Methods: At first, in order to determine the concentration of the Radix Trichosanthis component, the author investigated the viability of B16 melanoma cell. To measure the effects of Trichosanthes kirilowii extracts (n-BuOH, n-Hexane, EtOAc, H2O fractions) on the viability of A549 cells, A549 cells were treated with various concentrations (from 0.5 to $25{\;}\mu\textrm{g}/ml$) of components of Trichosanthes kirilowii. After 24hrs, the cell viability was measured by MTT assay. The EtOAc components of Trichosanthes kirilowii decreased the viability of A549 cells in a dose-dependent manner. H2O and n-BuOH components had no cell toxicity till $25{\;}\mu\textrm{g}/ml$, the n-hexane component showed minor cell toxicity at $25{\;}\mu\textrm{g}/ml$ and the EtOAc component cell toxicity was revealed at $5{\;}\mu\textrm{g}/ml$ concentration. Results: 1. The results of tyrosinase activity and the Radix Trichosanthis component; n-hexane and EtOAc components controlled it effectively; the n-BuOH components were less effective. 2. The results of melanin content analysis showed that the n-hexane and EtOAc components effectively inhibited, the n-BuOH fraction inhibited less, and H2O component didn't inhibit the terminal melanin formation. 3. In the n-BuOH and H2O component there were no changes, but in the n-hexane component the melanin content was effectively inhibited. 4. In the EtOAc fraction, although the melanin content was inhibited, the cell count was evidently suppressed, Of all of the Radix Trichosanthis components, the n-Hexane and EtOAc fractions inhibited the melanin synthesis best, but owing to its toxicity, the EtOAc components inhibited the cell count. Conclusion: The above results demonstrated that Radix Trichosanthis n-hexane fraction efficiently inhibited the tyrosinase activity and melanin synthesis.

• 한방안이비인후피부과학회지
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• 제17권1호
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• pp.82-93
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• 2004

Recently many efforts were focused to understanding the mechanical insights of melanogenesis to develop the agents for hyper-pigmentation and hypo-pigmentation. In the melanin biosynthetic pathway, tyrosinase is the rate limiting enzyme, and ${\alpha}$-melanocyte stimulating hormone(MSH) or cAMP-elevating agents stimulate melanogenesis and enhance the melanin synthesis and the tyrosinase activity. The author has analyzed the effects of Radix Trichosanthis on the basal Melanogenic activities of Bl6/F10 mouse melanoma cells, and on the ${\alpha}$-MSH or forskolin-induced melanogenesis. Radix Trichosanthis alone markedly suppressed melanin content and tyrosinase activity in a dose-dependent manner. Pretreatment of the cells with Radix Trichosanthis also suppressed the increase of ${\alpha}$-MSH(10 nM) or forskolin(20 ${\mu}$M)-induced melanin content and tyrosinase activity. The decrease in the tyrosinase activity was paralled by a decrease in the abundance of tyrosinase protein and tyrosinase promoter activity. Pretreatment of the cells with Radix Trichosanthis also inhibited the increase of forskolin(20 ${\mu}$M) induced the amount of tyrosinase protein and tyrosinase promoter activity. The results of DOPA staining revealed that pretreatment of the cells with Radix Trichosanthis showed less intensity than B16 melanoma cells stimulated with ${\alpha}$-MSH or forskolin. These results suggest that Radix Trichosanthis inhibits melanogenesis and abrogates ${\alpha}$-MSH and cAMP-induced melanogenesis in B16 melanoma cells.

• 한방안이비인후피부과학회지
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• 제17권1호
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• pp.131-142
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• 2004

The purpose of this research was to investigate cytotoxity of Sunbanhwalmyungeum extract. Cytotoxity was determined by MTT assay method. After tumor cell lines(G361, BI6F10, MDA, A549) transplantation, the extracts of SunBangHwalMyungEum and its composition oriental medicines were administered, cytotoxity was measured by absorbance. The results were obtained as follows. 1. Sunbanhwalmyungeum extract and its composition oriental medicines extracts showed the concentration was higher, the more cytotoxity increased. 2. Both water and ethanol extracts of Sunbanhwalmyungeum showed excellent cytotoxity against G361, B16F10, MDA, A549 and high cytotoxity over 80$\%$ against G361, B16F10, MDA except A549 at the concentration of 1000ppm. 3. In water extract, Rhei Radix et Rhizoma, Gleditsiae Spina, Trichosanthis Radix, Glycyrrhizae Radix, Ledebouriellae Radix showed excellent cytotoxity. In ethanol extract, Gleditsiae Spina, Citri Pericarpium, Trichosanthis Radix, Paeoniae Radix Rubra, Myrrha showed excellent cytotoxity. 4. Rhei Radix et Rhizoma, Gleditsiae Spina, Trichosanthis Radix, Paeoniae Radix Rubra showed high cytotoxity in both water and ethanol extrats. 5. In water extract, Glycyrrhizae Radix, Ledebouriellae Radix, Myrrha showed high cytotoxity against A361, Lonicerae Flos, Olibanum, Fritillariae cirrhosae Bulbus, Paeoniae Radix Rubra, Manitis Squama against B16F10, Paeoniae Radix Rubra, Manitis Squama against MDA, Rhei Radix et Rhizoma, Angelicae gigantis Radix against A549. 6. In ethanol extract, Lonicerae Flos, Trichosanthis Radix showed high cytotoxity against G361, Rhei Radix et Rhizoma, Angelicae gigantis Radix, Gleditsiae Spina, Olibanum, Angelicae dahuricae Radix, Fritillariae cirrhosae Bulbus, Paeoniae Radix Rubra, Glycyrrhizae Radix, Ledebouriellae Radix, Myrrha against B16F10, Rhei Radix et Rhizoma, Manitis Squama against MDA, Citri Pericarpium, Manitis Squama against A549.

• 대한예방한의학회지
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• 제3권1호
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• pp.83-100
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• 1999

This present study was carried out to investigate the effect of Saengjingamrotang and Saengjingamrotang plus Radix Trichosanthis in streptozotocin induced hyperglycemic rats. Body weight, serum levels of glucose insulin total cholesterol triglyceride and urine levels of volume glucose protein were measured in streptozotocin induced hyperglycemic rats orally receiving extracts of Saengjingamrotang and Saengjingamrotang plus Radix Trichosanthis for 4 days. According to above mentioned results, Saengjingamrotang and Saengjingamrotang plus Radix Trichosanthis have the effect of decreased blood sugar, serum lipid levels and urine volume, protein, glucose in streptozotocin induced hyperglycemic rats, and so they were expected to be appled to the treatment of diabetes mellitus.

• 한국응용과학기술학회지
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• 제39권3호
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• pp.417-422
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• 2022

쥐참외뿌리 추출물이 항산화 활성 및 피부 세포에서의 독성을 확인하여, 피부에 효과적으로 사용할 수 있는 기능성 소재로써의 활용 가능성을 확인해 보고자 하였다. 쥐참외뿌리 추출물의 항산화 활성의 지표가 되는 총 폴리페놀과 총 플라보노이드 함량을 확인하였고, 피부에서의 Neutral red assay를 이용하여 세포 독성을 확인하였다. 연구 결과, 총 폴리페놀과 총 플라보노이드의 함량은 농도 의존적으로 증가하였다. 섬유아 세포인 HDF cell에서의 높은 생존율이 확인되었으며, B16F10 melanoma cel와 RAW 264.7 cell에서는 5 ㎍/mL부터 세포 생존율이 유의하게 낮아지는 것이 확인되었다. 본 연구 결과는 쥐참외뿌리 추출물의 항산화 활성 및 피부 세포에서의 기초적인 자료로 사용가능할 것으로 사료되어 진다.

• 대한본초학회지
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• 제24권3호
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• pp.29-37
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• 2009

Objectives : The present study was carried out to investigate the effect of Trichosanthis Radix extract (TRE) on the proliferation and activation of eosinophils which were prepared from lung cells of asthma-induced mice by ovalbumin (OVA) treatment. Methods : C57BL/6 mouse was exposed to OVA three times a week for 6 weeks. The mouse lung tissues were dissected out, chopped and dossiciated with collagenase (1 $\mu$g/ml). Eosinophils were activated by rmIL-3/rmIL-5 co-treatments. The lung cells were treated with TRE, incubated for 48 hr at 37$^{\circ}C$, and analyzed by flow cytometer, ELISA and RT-PCR methods Results : To measure cytotoxicity, mouse lung fibroblast cells (mLFCs) were pretreated with various concentrations of TRE. TRE at 100 $\mu$g/ml, the highest concentration, examined did not have any cytotoxic effects on mLFCs. In FACS analysis, number of granulocyte/lymphocyte, CD3e-/CCR3+, CD3e+/CD69+, CD4+/CD8+ T cells in asthma-induced lung cells were significantly decreased by TRE treatment compared to the control group. But CD4+/CD25+ T cells were not examined significant change in lung cells treated with TRE. In ELISA analysis, production levels of IL-3, IL-5, IL-13 and histamine in asthma-induced lung cells, which were induced by rIL-3 plus rmIL-5 co-treatment, were significantly decreased by TRE treatment. Conclusions : The present data suggested that Trichosanthis Radix on the inhibition of parameters associated with asthma responses in eosinpophils, and thus implicate the possibility for the clinical application of Trichosanthis Radix.

• 한국응용과학기술학회지
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• 제39권4호
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• pp.580-587
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• 2022

본 연구에서는 쥐참외뿌리 추출물의 항산화 활성 및 미백, 항염증 활성에 대한 생리활성 효과와 기능성 소재로써의 활용 가능성을 확인해 보고자 하였다. 쥐참외뿌리 추출물의 DPPH 라디칼 소거 활성을 통한 항산화 활성, 멜라닌 세포인 B16F10 melanoma 세포에 대한 멜라닌 생성 억제능을 통한 미백 활성 효과, 대식 세포인 RAW 264.7 세포에 대한 NO 생성 억제능을 통한 항염증 활성 효과를 확인해 보고자 하였다. 연구결과, 쥐참외뿌리 추출물은 농도 의존적으로 DPPH 라디칼 소거활성이 확인되었으며, 양성 대조군인 Ascorbic acid와 비슷한 DPPH 라디컬 소거활성이 확인되었다. 100 nM 𝛼-MSH로 유도된 멜라닌 생성 억제 활성과 LPS 1 ㎍/mL로 유도된 NO 생성 억제능을 유의하게 억제 하는 것을 확인되었다. 이에 따라 쥐참외뿌리 추출물은 항산화 및 미백, 항염증 효과를 가진 기능성 소재로써의 활용 가능성이 있음이 사료되어 진다.