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Antioxidant Enzyme Activities in Tissues of Papilio xuthus during Pupal Stage (호랑나비의 용기 동안 조직 내 항산화 효소의 활성)

  • Shin, Myung-Ja;Kim, Kyoung-Keun;Lim, Jae- Hwan;Jeong, Hyung-Jin;Seo, Eul-Won
    • Journal of Life Science
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    • v.19 no.2
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    • pp.228-233
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    • 2009
  • The purpose of this study is to evaluate the activities of five different antioxidant enzymes in various tissues of Papilio xuthus during pupal stage. Superoxide dismutase (SOD) activity in haemolymph was the highest just after pupation and then decreased gradually until 7 days after pupation but the activity in other tissue was constant during metamorphosis. This result indicates that primary antioxidant process of reactive oxygen species proceed in haemolymph. Catalase (CAT) activity in studied tissues was also the highest just after pupation and its relative activity was also high during pupal stage, suggesting that CAT is the primary enzyme in catalysis of hydrogen peroxide. Glutathion peroxidase (GPX) activity was constant and its relative activity was very low in all tested tissues. Glutathione S-transferase (GST) activity in haemolymph was high at 3 days and 5 days after pupation, and the activity in fat body was the highest at the 1 day after pupation and then decreased gradually for 7 days after pupation. Glutathion reductase (GR) activity in haemolymph and fat body was high at 1 day after pupation, but relatively low GR activity was detected in the rest tissues. Based on these results, GST activity was higher than that of GPX and GR, and it is also believed that GST was more involved in reduction process through lipid peroxidation than GPX.

Morphology of RF-sputtered Mn-Coatings for Ti-29Nb-xHf Alloys after Micro-Pore Form by PEO

  • Park, Min-Gyu;Park, Seon-Yeong;Choe, Han-Cheol
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2016.11a
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    • pp.197-197
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    • 2016
  • Commercially pure titanium (CP Ti) and Ti-6Al-4V alloys have been widely used for biomedical applications. However, the use of the Ti-6Al-4V alloy in biomaterial is then a subject of controversy because aluminum ions and vanadium oxide have potential detrimental influence on the human body due to vanadium and aluminum. Hence, recent works showed that the synthesis of new Ti-based alloys for implant application involves more biocompatible metallic alloying element, such as, Nb, Hf, Zr and Mo. In particular, Nb and Hf are one of the most effective Ti ${\beta}-stabilizer$ and reducing the elastic modulus. Plasma electrolyte oxidation (PEO) is known as excellent method in the biocompatibility of biomaterial due to quickly coating time and controlled coating condition. The anodized oxide layer and diameter modulation of Ti alloys can be obtained function of improvement of cell adhesion. Manganese(Mn) plays very important roles in essential for normal growth and metabolism of skeletal tissue in vertebrates and can be detected as minor constituents in teeth and bone. Radio frequency(RF) magnetron sputtering in the various PVD methods has high deposition rates, high-purity films, extremely high adhesion of films, and excellent uniform layers for depositing a wide range of materials, including metals, alloys and ceramics like a hydroxyapatite. The aim of this study is to research the Mn coatings on the micro-pore formed Ti-29Nb-xHf alloys by RF-magnetron sputtering for dental applications. Ti-29Nb-xHf (x= 0, 3, 7 and 15wt%, mass fraction) alloys were prepared Ti-29Nb-xHf alloys of containing Hf up from 0 wt% to 15 wt% were melted by using a vacuum furnace. Ti-29Nb-xHf alloys were homogenized for 2 hr at $1050^{\circ}C$. Each alloy was anodized in solution containing typically 0.15 M calcium acetate monohydrate + 0.02 M calcium glycerophosphate at room temperature. A direct current power source was used for the process of anodization. Anodized alloys was prepared using 270V~300V anodization voltage at room. Mn coatings was produced by RF-magnetron sputtering system. RF power of 100W was applied to the target for 1h at room temperature. The microstructure, phase and composition of Mn coated oxide surface of Ti-29Nb-xHf alloys were examined by FE-SEM, EDS, and XRD.

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Demand Surveys for Big Research Facilities and Equipments to Advance National S&T Research Infrastructure (과학기술 하부구조 선진화를 위한 대형 연구장비의 수요 조사)

  • 권용수;민철구
    • Proceedings of the Technology Innovation Conference
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    • 1997.12a
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    • pp.159-176
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    • 1997
  • This paper deals with demand surveys for big science and technology research facilities and equipments to advance national S'||'&'||'T research infrastructure. We perform surveys thrice based on applied Delphi method on the future demand of big S'||'&'||'T research facilities and equipments among Korean scientists and engineers. We employ the concept of big S'||'&'||'T research facilities and equipments as follows: \circled1 The operating size of it is equivalent to that of an institute or research center, and/or \circled2 The users in various disciplines are many, and/or \circled3 The application areas or spill-over effects are large, and/or \circled4 The scale and scope of research objects is equivalent to that of mega science area such as earth.oceanography.space, and/or \circled5 The expenses for installing and operating it are to be supported by government, and/or \circled5 The facilities are expected as necessary for international joint research, and/or \circled7 It is necessary for promoting creative basic science and developing creative technology. We ask the respondents to answer the following questionnaire: - How to prioritize the equipments according to the degree of importance\ulcorner $\square$ Promotion of basic science and mega science, the development of the technologies to enhance the public welfare, the competitiveness of industrial technologies, the job creation for the S'||'&'||'T personnel, and international cooperation. - Who should be in charge of acquisition and operation of the equipments\ulcorner $\square$ Industry, Government Research Institutes, Academy, ERC and SRC. - When shall we acquire the equipment\ulcorner $\square$ Within 2000, 2002, 2007, 2012, and 2017. - How shall we acquire the equipments\ulcorner $\square$ International Joint Development, Domestic Development, Acquisition from Overseas, - How much will the equipment generate spill-over effects to national competitiveness\ulcorner $\square$ Promotion of basic science, contribution to the economy, supply of S'||'&'||'T personnel, and international cooperation. We suggest the following equipments as prioritized candidates after consulting the officers from MOST, MOE, MIC, MOEN and experts from KBSI and STEPI:(table omitted) where, #1, Korea Advanced Liquid Metal Reactor, #2. 800 MHz Superconduction Fourier-Transform Nuclear Magnetic Resonance Spectrometer, #3. Ion Accelerator, #4. Seismic Test Facility, #5. Transonic Wind Tunnel, #6. Radio Telescope for Very Long Baseline Interferometer, #7. 3000t Universal(or Large Structure) Testing Machine, #8. Compost Facility or Plasma Pyrolysis Facility.

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PHOTOTOXIC EFFECTS OF LOW LEVEL LASER IRRADIATION ON HUMAN OSTEOSARCOMA CELLS (골육종세포에 미치는 레이저 조사의 광독성 효과)

  • Son, Jang-Ho;Cho, Young-Chul;Ryu, Sung-Ho;Kim, Gyoo-Cheon;Sung, Iel-Yong;Park, Bong-Soo
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.30 no.6
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    • pp.509-515
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    • 2004
  • Photodynamic therapy(PDT) has advanced to clinical trials for the treatment of a variety of solid tumors and presents an alternative treatment option for tumors resistant to chemo-and/or radio-therapy. PDT is based on the combination of laser light of appropriate wavelength and energy to activate a systemically or locally applied photosensitizer that concentrates preferentially in malignant tissues. In this study, phototoxicity of laser EIT 21 was analysed in human osteosarcoma cell(HOS) and the second objective of this study was to determine the ability of laser EIT 21 to induce apoptosis. This study demonstrated that laser EIT 21 had a phototoxicity to HOS cells. In order to examinate whether cell death was induced by necrosis or apoptosis, variety of techniques which assess apoptosis were used. TUNEL assay showed only a few the positive reaction on condensed nuclei. It is hard to find condensed or fragmented nuclei on HOS cells irradiated with laser EIT 21 in Hemastat and AO/EB stain. By DNA electrophoresis, cells also did not show DNA degradation characteristic of apoptosis with a ladder pattern of DNA fragments. Apoptosis-related factors were analyzed by western blotting. The expression of p53 was constant and cells irradiated with laser did not show the caspase-3 and PARP degradation, therefore we suggest that p53 and caspase-3 are not involved in laserinduced cell death.

Broadband Multi-Layered Radome for High-Power Applications (고출력 환경에 적용 가능한 광대역 다층 구조 레이돔)

  • Lee, Ki Wook;Lee, Kyung Won;Moon, Bang Kwi;Choi, Samyeul;Lee, Wangyong;Yoon, Young Joong
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.29 no.1
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    • pp.50-60
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    • 2018
  • In this paper, we developed a broadband multi-layered radome applicable for high-power applications. In this regard, we presented the wave propagation characteristics of the broadband multi-layered radome with the ABCD matrix and obtained the optimal thickness and the material constant for each layer by an optimization algorithm called "particle swarm optimization," implemented by a commercial numerical modeling tool. Further, we redesigned it in view of mechanical properties to reflect environmental conditions such as wind, snow, and ice. The power transmission property was reanalyzed based on the recalculated data of each layer's thickness to consider the limitations of the fabrication of a large structure. Under the condition of a peak electric field strength that is 10 dB above the critical electric field strength in air breakdown, we analyzed the air breakdown by radio frequency(RF) in the designed radome using the commercial full-wave electromagnetic tool. The radome was manufactured and tested by continuous wave(CW) RF small signal and large signal in an anechoic chamber. The test results showed good agreement with those attained by simulation.

Inactivation of Xanthomonas citri subsp. citri and Effect on Infection of Citrus Canker by Gamma Irradiation (감마선에 의한 감귤 궤양병균의 불활성화와 감귤 궤양병 감염에 미치는 영향)

  • Kim, Kyung Nam;Song, Min A;Han, Sang Heon;Song, Seong Joon;Jeun, Yong Chull
    • Research in Plant Disease
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    • v.20 no.4
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    • pp.283-288
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    • 2014
  • Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) has been quarantined by many countries in the world. Recently, the usage of methyl bromide should be limited, application by gamma irradiation on the agricultural production is raised as an alternative method. In this study, the level of gamma irradiation which could decrease of population of Xcc in the suspension or on the surface of citrus fruit was investigated. The $D_{10}$ value of Xcc, which is radiation dose required to reduce the number of the microorganism, was 55 and 28 Gy in the suspension and on the surface of citrus fruit, respectively. Furthermore, disease severity was suppressed on the citrus leaves inoculated with Xcc suspension pre-treated with gamma irradiation. Based on this study, it is suggested that Xcc on the citrus fruit could be eradicated by gamma irradiation and the results of this study may be valuable for application of gamma ray in quarantine activity.

Functional Improvement of Hot Melt Adhesive Using Polyamide Type Resin - (II) The Effects of Terpene Resin - (폴리아미드계 수지를 이용한 핫멜트 접착제의 기능향상 - (II) 테르펜수지의 영향 -)

  • Chung, Kyung-Ho;Hong, Young-Keun;Chun, Young-Sik
    • Applied Chemistry for Engineering
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    • v.9 no.2
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    • pp.226-231
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    • 1998
  • Hot melt adhesive which is solventless type has been widely used due to the possibility of automated adhesion process. The main purpose of this study is the development of polyamide based hot melt adhesive to improve the property of conventional ethylene-vinyl acetate hot melt adhesive, which has an inherent problem against heat resistance. In this study, it was found that the terpolymers of nylon 6, nylon 66, and nylon 12(CM831, 843P types) instead of nylon homopolymer were suitable base resins for hot melt adhesive, since the disruption of regularity in the polymer chains reduced the crystallinity, resulting in lower melting point and melt viscosity. According to the results, the optimum adhesion property could be obtained by the using 75/25~50/50 weight radio of CM831/843P resin as a base resin. Terpene resin was used as tackifier to improve adhesion and wetting properties. The best result can be obtained with the 10 wt.% addition of terpene resin. The terpene resin acted as proper tackifier in this system which decreased the melt temperature and viscosity, but increased the mechanical strength of adhesive itself. Also, the rheological property of the adhesive changed from typical non-Newtonian behavior to Newtonian behavior as terpene resin was added.

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Study on Recognition and Practice of Breastfeeding of mothers of under toddler (영유아 어머니의 모유수유에 대한 인식 및 실천에 관한 연구)

  • Song, Ji-Ho;Kim, Soon-Ae;Moon, Young-Soo;Park, Young-Joo;Paik, Seng-Nam;Pyun, Soo-Ja;Lee, Nam-Hee;Cho, Kyul-Ja;Cho, Hee-Sook;Choi, Mi-Hyae;Choi, Yang-Ja
    • 모자간호학회지
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    • v.3 no.2
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    • pp.153-165
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    • 1993
  • This study presents results of surveys conducted Seoul area using structured questionnaire developed by researchers to determine the degree of recognition and practice of breast-feeding. The subjects were mothers of 255 childs 1-36 months of age who have experienced breast feeding over one month. Recognition of breastfeeding was significantly associated with "baby's health", "emotional relationship between mother and infant", "mother's health", "for economic reasons". The majority of mothers got information of breastfeeding through booklets(64.7%), from her mothers(44.7%), through T.V or Radio (45.5%), School education(41.6%) from mother in law(36.5%), and did not perceive professional advice from nurses or doctors as important. "Lack of milk" was the predominant reason for for terminating breastfeeding. Efforts for practice of breastfeeding was comparatively high because of experienced mothers of breastfeeding. Some of them were "frying to take liguid diet and nurtitional food for increasing secretion of milk", "fed on by infant-demanded schedule". Duration of breastfeeding was under on month (31.0%), 1-3 months(25.5%), 3-6 months(19.6%), 6-9 months (11.0%). Degree of recognition and practice of breastfeeding was significantly correlated with mother's educational background, location of delivary(big hopital), Rooming in, over 5 months of breastfeeding. Based on the results of this study, we suggest that effective and supportive breastfeeding program developed by nurses have to provide to mothers of infants to take an important role in breastfeeding.

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Verification of MCNP/ORIGEN-2 Model and Preliminary Radiation Source Term Evaluation of Wolsung Unit 1 (월성 1호기 MCNP/ORIGEN-2 모델 검증 및 예비 선원항 계산)

  • Noh, Kyoungho;Hah, Chang Joo
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.13 no.1
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    • pp.21-34
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    • 2015
  • Source term analysis should be carried out to prepare the decommissioning of the nuclear power plant. In the planning phase of decommissioning, the classification of decommissioning wastes and the cost evaluation are performed based on the results of source term analysis. In this study, the verification of MCNP/ORIGEN-2 model is carried out for preliminary source term calculation for Wolsung Unit 1. The inventories of actinide nuclides and fission products in fuel bundles with different burn-up were obtained by the depletion calculation of MCNPX code modelling the single channel. Two factors affecting the accuracy of source terms were investigated. First, the neutron spectrum effect on neutron induced activation calculation was reflected in one-group microscopic cross-sections of relevant radio-isotopes using the results of MCNP simulation, and the activation source terms calculated by ORIGEN-2 using the neutron spectrum corrected library were compared with the results of the original ORIGEN-2 library (CANDUNAU.LIB) in ORIGEN-2 code package. Second, operation history effect on activation calculation was also investigated. The source terms on both pressure tubes and calandria tubes replaced in 2010 and calandria tank were evaluated using MCNP/ORIGEN-2 with the neutron spectrum corrected library if the decommissioning wastes can be classified as a low level waste.

Use of Unmanned Aerial Vehicle for Multi-temporal Monitoring of Soybean Vegetation Fraction

  • Yun, Hee Sup;Park, Soo Hyun;Kim, Hak-Jin;Lee, Wonsuk Daniel;Lee, Kyung Do;Hong, Suk Young;Jung, Gun Ho
    • Journal of Biosystems Engineering
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    • v.41 no.2
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    • pp.126-137
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    • 2016
  • Purpose: The overall objective of this study was to evaluate the vegetation fraction of soybeans, grown under different cropping conditions using an unmanned aerial vehicle (UAV) equipped with a red, green, and blue (RGB) camera. Methods: Test plots were prepared based on different cropping treatments, i.e., soybean single-cropping, with and without herbicide application and soybean and barley-cover cropping, with and without herbicide application. The UAV flights were manually controlled using a remote flight controller on the ground, with 2.4 GHz radio frequency communication. For image pre-processing, the acquired images were pre-treated and georeferenced using a fisheye distortion removal function, and ground control points were collected using Google Maps. Tarpaulin panels of different colors were used to calibrate the multi-temporal images by converting the RGB digital number values into the RGB reflectance spectrum, utilizing a linear regression method. Excess Green (ExG) vegetation indices for each of the test plots were compared with the M-statistic method in order to quantitatively evaluate the greenness of soybean fields under different cropping systems. Results: The reflectance calibration methods used in the study showed high coefficients of determination, ranging from 0.8 to 0.9, indicating the feasibility of a linear regression fitting method for monitoring multi-temporal RGB images of soybean fields. As expected, the ExG vegetation indices changed according to different soybean growth stages, showing clear differences among the test plots with different cropping treatments in the early season of < 60 days after sowing (DAS). With the M-statistic method, the test plots under different treatments could be discriminated in the early seasons of <41 DAS, showing a value of M > 1. Conclusion: Therefore, multi-temporal images obtained with an UAV and a RGB camera could be applied for quantifying overall vegetation fractions and crop growth status, and this information could contribute to determine proper treatments for the vegetation fraction.