• The Korean Journal of Ecology
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• v.12 no.3
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• pp.161-170
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• 1989

To verify allelopathic effects of Artemisia princeps var. orientalis, a number of laboratory experiments have been performed. Aqueous extracts of leaves, stems and roots of the above species inhibited the seed germination and seedling growth of experimental species, receptor plants. In general, the higher was the concentration of the extracts, the lower was the germination and the growth ratio. Volatile substances released from leaves of the donor plants also inhibited the seed germination and the radicle elongation of receptor plants. Therefore, to find out the inhibitory substances emitted from the wormwood, gas chromatography was employed. As a result of the analysis, 17 KDICical substances were isolated from the leaves and most of them were identified as phenolic compounds.

• Journal of Ginseng Research
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• v.9 no.2
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• pp.146-153
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• 1985

This study was conducted to know the effect of vinyl mulching from the sowing to germination on the seedbed for germination and production of seedling. Embryo growth was restrained with decreasing the water content in seed and stopped below 10% water content. Germination was possible over 55% water content but radicle growth was stopped at 55% water content. Ratioes of embryo / emdosperm length were about 50% at seeding time, and about 80% at just before freeing season, and the ratio was increased from the thawing season again. Vinyl mulching increased the soil water content and soil temperature. Germination rate and number of available seedling in vinyl mulching were increased 10% and 12%, respectively.

• Plant Biotechnology Reports
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• v.3 no.3
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• pp.199-203
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• 2009

Rugosa rose (Rosa rugosa) is cultivated as a garden flower and an important genetic resource for the breeding of roses (R. hybrida). This study describes culture conditions for high frequency plant regeneration from zygotic embryo explants via somatic embryogenesis in rugosa rose. Mature zygotic embryo, cotyledon, and radicle explants formed embryogenic calluses at frequencies of 38, 6.7, and 8.8% when cultured on half-strength Murashige and Skoog medium (${\frac{1}{2}}MS$) supplemented with 2.26, 9.05, and $9.05{\mu}M$ 2,4-dichlorophenoxyacetic acid, respectively. Embryogenic calluses produced numerous somatic embryos, which then developed into plantlets on ${\frac{1}{2}}MS$ without growth regulators. Regenerated plantlets were grown to whole plants in a growth chamber.

• Journal of Life Science
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• v.10 no.6
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• pp.598-604
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• 2000

This study was conducted to investigate the effect of simulated acid rain (SAR) on germination, growth, acid buffering capacity and nutrient leaching in Impatiens balsamina L. and Tagetes patula L.. In both species, germination was not inhibited at pH 4.0, but the germination rate decreased at the lower pH of 3.0, showing higher rate in Inpatiens balsamina L. than Tagetes patula L.. As the pH decreases, the growth of radicle was markedly decreased than that of hypocotyl in both species. The plant height, root length, leaf area, total dry weight, relative growth rate and net assimilation rate were inhibited by SAR. The acid buffering capacity in the leaves were increased at pH 4.0, on the other hand, it was shown a tendency to decrease at pH 2.0 in both species. As the pH levels decreased from 5.6 to 2.0, the nutrient leaching from leaves was significantly increased in both species. Based on the results, there are a great difference in the responses to SAR between the two species. In general, Tagetes patula L. represented a higher tolerance to SAR than Impatiens balsamina L.. These results suggested that interspecific variation in the acid buffering capacity and nutrient leaching from leaves may be responsible for the interspecific susceptibility to SAR.

• Korean Journal of Soil Science and Fertilizer
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• v.16 no.3
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• pp.280-285
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• 1983

The effect of pH and calcium on the growth of subterraneum clover (Trifolium subteraneum L.) varieties Dwalganup, Mt. Barker, and Tallarook was determined in solution culture. At pH 4.5 and below all components of growth except germination percentage were adversely affected. The effect of lower pH was improved at high calcium level(3.0mM). There was a varietal difference in response to low pH. Mt. Barker showed the highest percent germination and produced the longest radicle length, whereas the greatest value in top dry weight, root dry weight, root length and number of laterals was obtained with Tallarook.

• The Korean Journal of Ecology
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• v.12 no.4
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• pp.219-236
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• 1989

The effect of salinity on the seed germination and seedling growth of Pinus densiflora for. erecta Uyeki were studied under the controlled conditions in the growth chamber. The seeds were sorted into three classes in weight such as large ($15.49\pm$1.63mg), medium($10.61\pm$1.38mg), and small ($6.57\pm$1.33mg) to determine the role of seed weight in germination and seedling growth of the pine. Polymorphic seeds of the pine were germinated an salinity range of 0 to 1.5% NaCl under various temperature ($10^{\circ}$ $-25^{\circ}$ with $5^{\circ}$C interval of constant temperature, and $10^{\circ}$ $-20^{\circ}$C, $15^{\circ}$ $-25^{\circ}$C of alternating temperature) in order to determine their germinability and seeding growth. In control plot, there was little difference of germination percentage among the seed weight classes, but in saline plot, the larger seeds generally had a higher percentage and rate of germination. There occurred synergistic interaction between salinity and temperature in the germination and the increase of temperature enhanced germination of seeds at the same salinity level. Alternating temperature regimes of $15^{\circ}$-$25^{\circ}$C yielded maximum germination and no germination was occurred at $10^{\circ}C$. The germination at alternating temperature showed higher germination percentage than at constant temperature. The percentage and of germination decreased drastically with increased salinity level to 1.00%; no germination was occurred at 1.50% salinity level. The growth fo sddelings from larger seeds was better than that of smaller seeds at the same salinity and temperature. The hypocotyl and radicle were more sensitive than cotyledon to the increased salinity stress.

• Journal of Plant Biotechnology
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• v.48 no.1
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• pp.44-53
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• 2021

The present work aims to study the induction of somatic embryogenesis in cork oak (Quercus suber L.) from immature zygotic embryos and young apical leaves obtained from 2-month-old seedlings through acorn germination on sterilized peat. The immature zygotic embryos were grown for 1 month on the mineral solution of MS in the presence of 4.52 µM 2,4-D and 30 g/L sucrose. They were then transferred to the same mineral solution with no added growth regulators. In the third subculture, yellow somatic embryos, characterized by two voluminous cotyledons, were differentiated from the radicle of the immature zygotic embryos. The induction of somatic embryogenesis in young leaves required a series of transfers on different culture media containing 30 g/L sucrose and 100 mg/L myo-inositol. Secondary or recurrent somatic embryogenesis occurred within the immature somatic embryo radicles after 1 month of culture on growth regulator-free medium containing WPM macronutrients, MS micronutrients, and vitamins.

• Journal of Korean Society of Forest Science
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• v.28 no.1
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• pp.36-42
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• 1975

In the beginning of the germination of Castanea creata seeds, seedlings with identical genetic characteristics from only one seed were produced by longitudinally splitting the radicle and epicotyl in half with a razor blade. The results are summarized as follows: 1. 2-8 genetically identical Castanca crenata seedlings were produced by longitudinally splitting the radicles and epicotyles from one to four in number in half with a razor blade 1-3 times about 7 days apart, while the seeds were germinating. 2. The proportion of success of splitting seedlings was very high (90-100%). When some of the spliting seedlings were withered a sprout appeared again and it was possible the seedling to revive. 3. The production rate, growth and T/R rate of seedlings which were split only once were lower that those of the seedlings which were split several times. 4. The growth and T/R rate of split seedlings which were one of no more than four seedlings produced from one seed showed no difference in comparison with normal seedlings, but in seedlings which were one of more than four, the growth and T/R rate were considerably decreased. 5. With the progress of growth, the external shapes of splitting seedlings completely returned to the original state, and no problems in growth, were found.

• Korean Journal of Plant Resources
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• v.19 no.1
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• pp.169-173
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• 2006

The effects of aqueous extract and volatile substances of 2 Angelica plants on seed germination and seedling growth were investigated. The seed germination of Angelica gigas showed increase in proportion to increase in aqueous extract concentration of A. gigas, while that of Angelica acutiloba was reduced proportionally to the extract concentration. The seed germination of A. gigas and A. acutiloba treated with aqueous extract of A. acutiloba was inhibited. The seedling elongation of A. gigas and A. acutiloba was slightly increased at lower concentration of aqueous extract of A. gigas, whereas it was proportionally decreased at higher concentrations. The seedling elongation of 2 Angelica plants was decreased by the aqueous extract of A. acutiloba. The aqueous extract of A. acutiloba caused significant inhibition in seedling growth of 2 Angelica plants. The seed germination of Lactuca sativa was not affected by volatile substances emitted from 2 Angelica plants. The radicle elongation of L. sativa treated with volatile substances of 2 Angelica plants was inhibited slightly and it was not suppressed according to the concentration of volatile substances.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.2
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• pp.112-117
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• 2000

This study was conducted to evaluate the drought tolerance of Japonica and Indica rice cultivars during germinating and seedling stages by using the polyethylene glycol (PEG) solution. Each 5 cultivars of Japonica and Indica were cultured from 14 days after seeding(DAS) to 21 DAS using the PEG solution in a moderate water potential (-0.63 MPa). The lengths of radicle and plumule during the germinating stage were inhibitied by the PEG treatment to about 50% and 85%, respectively. The application of PEG to the seedling of two rice types caused to inhibit the plant height and leaf age about 23 % and 10%, respectively. Shoot and root dry weights by PEG treatment were inhibited more severely in Japonica than those in Indica. The difference on delaying of leaf area expansion between both rice types was not found with treatment of PEG, while the leaf color was increased in both Japonica and Indica by 19.9% and 9.2%, respectively. The average photosynthetic ability was inhibited more in Japonica to 36.0% than did Indica to 27.9%. The stomatal conductance was severely affected by PEG treatment, and the degree was varied in both rice types, ranged with 80-85% in Japonica and 29.3-81.6% in Indica. These results indicate there is little relationship between seed germination and seedling growth under the stress of low water potential.