Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Induction of somatic embryogenesis from immature zygotic embryos and young apical leaves in cork oak (Quercus suber L.)

  • El Ansari, Zineb Nejjar (Biology Department, Faculty of Sciences, Abdelmalek Essaadi University) ;
  • El Bouzdoudi, Brahim (Environmental Technologies, Biotechnology and Bio-resources Valorisation Team (ETBBV), Department of Biology, Faculty of Science and Technology Al Hoceima, Abdelmalek Essaadi University) ;
  • Errabii, Tomader (Biology Department, Faculty of Sciences, Abdelmalek Essaadi University) ;
  • Saidi, Rabah (Sciences of Matter and Life Department, Tetouan Higher Teacher Training College of Tetouan, Abdelmalek Essaâdi University) ;
  • El Kbiach, Mohammed L'bachir (Biology Department, Faculty of Sciences, Abdelmalek Essaadi University)
  • Received : 2021.01.14
  • Accepted : 2021.03.24
  • Published : 2021.03.31
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Abstract

The present work aims to study the induction of somatic embryogenesis in cork oak (Quercus suber L.) from immature zygotic embryos and young apical leaves obtained from 2-month-old seedlings through acorn germination on sterilized peat. The immature zygotic embryos were grown for 1 month on the mineral solution of MS in the presence of 4.52 µM 2,4-D and 30 g/L sucrose. They were then transferred to the same mineral solution with no added growth regulators. In the third subculture, yellow somatic embryos, characterized by two voluminous cotyledons, were differentiated from the radicle of the immature zygotic embryos. The induction of somatic embryogenesis in young leaves required a series of transfers on different culture media containing 30 g/L sucrose and 100 mg/L myo-inositol. Secondary or recurrent somatic embryogenesis occurred within the immature somatic embryo radicles after 1 month of culture on growth regulator-free medium containing WPM macronutrients, MS micronutrients, and vitamins.

Keywords

References

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