• Journal of Life Science
• /
• v.21 no.9
• /
• pp.1205-1213
• /
• 2011

The optimal concentrations of salts in medium for cell growth and the production of carboxymethylcellulase (CMCase) by a recombinant E. coli JM109/DL-3 were established using two statistical methods: orthogonal array method (OAM) and response surface method (RSM). The analysis of variance (ANOVA) of data based on OAM indicated that $K_2HPO_4$ gave maximum sum of square (S) and percentage contribution (P) for cell growth as well as production of CMCase. The optimal concentrations of $K_2HPO_4$, NaCl, $MgSO_4{\cdot}7H_2O$, and $(NH_4)_2SO_4$ in medium for cell growth extracted by Qualitek-4 (W32b) Software were 10.0, 1.0, 0.2, and 0.6 g/l, respectively, whereas those for the production of CMCase by E. coli JM109/DL-3 were 5.0, 1.0, 0.4, and 0.6 g/l. The analysis of variance (ANOVA) resulting from RSM indicated that a highly significant salt for cell growth was $K_2HPO_4$ ("probe>F" less than 0.0001), whereas $K_2HPO_4$ and $MgSO_4{\cdot}7H_2O$ were significant for the production of CMCase. The optimal concentrations of $K_2HPO_4$, NaCl, $MgSO_4{\cdot}7H_2O$, and $(NH_4)_2SO_4$ for cell growth extracted by Design Expert Software were 7.44, 1.08, 0.22, and 0.88 g/l, respectively, whereas those for production of CMCase were 5.84, 0.69, 0.28, and 0.54 g/l. The optimal concentrations of salts and their influences on cell growth and production of CMCase extracted by OAM were almost the same as those by RSM. Production of CMCase by a recombinant E. coli JM109/DL-3 under optimized concentration of salts was 1.93 times higher than that by Bacillus amyloliquifaciens DL-3.

• Journal of Animal Science and Technology
• /
• v.53 no.3
• /
• pp.253-259
• /
• 2011

The aim of this work was to determine the method and predict the optimum conditions for egg quality stored for 7 days when combination treatments of irradiation and chitosan coating were applied using response surface methodology (RSM). A central composite design was chosen for the RSM in this study and the factors were irradiation dose (0~2 kGy) and concentration of chitosan coating material (0~2%). Performance of the irradiation and chitosan coating were evaluated by analyzing the egg quality and functional property factors. The predicted maximum level of Haugh units and foaming ability calculated by a developed model were 74.19 at 0 kGy of irradiation with coating by 0.96% chitosan solution and 50.83 mm at 2.0 kGy with 1.01%, respectively. The predicted minimum value of foam stability and 2-thiobarbituric acid reactive substances (TBARS) value were 2.97 mm at 0.39 kGy with 0.21% and 0.54 mg malonaldehyde/kg egg yolk at 0 kGy with 0.90% of chitosan solution, respectively. Results clearly showed that gamma irradiation negatively affected the Haugh unit and TBARS but positively affected the foaming capacity. The estimated value from the developed model by RSM was verified by no statistical difference with observed value. Therefore, RSM can be a good tool for optimization and prediction of egg quality when 2 or more treatments are combined. However, one should decide the target quality first to achieve a successful implementation of this technology.

• Journal of the Korean Society for Precision Engineering
• /
• v.29 no.12
• /
• pp.1368-1375
• /
• 2012

In this paper, a method for prediction of spread is proposed to design proper roll profile taking into account spread in shape rolling of angle bar. The effect of the process variables on spread, such as draught ratio, bending angle and aspect ratio, is analyzed by FE-analysis and response surface method (RSM). Roll profiles for equal angle bar are designed with the spread predicted by the regression equation. Effectiveness of the designed roll profiles are verified by FE-analysis in which the flange length, strain distribution, mean strain and roll torque are compared with those by Geuze. Finally, the proposed method is applied to the design of roll profile for unequal angle bar. As a result, the final product can be obtained within the allowable tolerance of ${\pm}0.5mm$ in length. Therefore, it is found that the prediction of spread can improve the efficiency of design roll profile in shape rolling of angle bar.

• 한국생물공학회:학술대회논문집
• /
• 2003.04a
• /
• pp.228-232
• /
• 2003

Lovastatin produced by Aspergillus terreus via polyketide pathway is a secondary metabolite with high anti-hypercholesterolemic activity. In this paper we are going to present effective strain development strategies for lovastatin production by comparing the productivity of the mutants obtained through traditional rational screening process and protoplast fusion method. Mutants resistant against various antibiotics and/or antimetabolites showed significantly higher lovastatin productivity than the corresponding mother strains, demonstrating that rational screening method was very efficient in selecting high yielding producers. Recombinant fusants obtained using protoplast fusion between high producers were observed to have very different morphology and physiology as represented by the production and secretion of lovastatin, as well as cell growth pattern. In parallel with the strain development, optimization process for the production medium was carried out in order to find optimal concentrations of the medium components using such a powerful statistical method as response surface method (RSM). It was concluded that not only the optimum production medium but also good morphological characteristics of the high-yielding producers led to higher lovastatin production.

• 한국전산유체공학회:학술대회논문집
• /
• 2005.10a
• /
• pp.163-167
• /
• 2005

A research to evaluate efficiency of design optimization was performed for aerodynamic design optimization problem in distributed computing environment. The aerodynamic analyses which take most of computational work during design optimization were divided into several jobs and allocated to associated PC clients through network. This is not a parallel process based on domain decomposition rather than a simultaneous distributed-analyses process using network-distributed computers. GBOM(gradient-based optimization method), SAO(Sequential Approximate Optimization) and RSM(Response Surface Method) were implemented to perform design optimization of transonic airfoil and to evaluate their efficiencies. One dimensional minimization followed by direction search involved in the GBOM was found an obstacle against improving efficiency of the design process in distributed computing environment. The SAO was found quite suitable for the distributed computing environment even it has a handicap of local search. The RSM is apparently the fittest for distributed computing environment, but additional trial and error works needed to enhance the reliability of the approximation model are annoying and time-consuming so that they often impair the automatic capability of design optimization and also deteriorate efficiency from the practical point of view.

• Journal of computational fluids engineering
• /
• v.11 no.2 s.33
• /
• pp.19-24
• /
• 2006

A research to evaluate the efficiency of design optimization was carried out for aerodynamic design optimization problem in distributed computing environment. The aerodynamic analyses which take most of computational work during design optimization were divided into several jobs and allocated to associated PC clients through network. This is not a parallel process based on domain decomposition in a single analysis rather than a simultaneous distributed-analyses using network-distributed computers. GBOM(gradient-based optimization method), SAO(Sequential Approximate Optimization) and RSM(Response Surface Method) were implemented to perform design optimization of transonic airfoils and evaluate their efficiencies. dimensional minimization followed by direction search involved in the GBOM was found an obstacle against improving efficiency of the design process in the present distributed computing system. The SAO was found fairly suitable for the distributed computing environment even it has a handicap of local search. The RSM is apparently the most efficient algorithm in the present distributed computing environment, but additional trial and error works needed to enhance the reliability of the approximation model deteriorate its efficiency from the practical point of view.

• Journal of Korean Society for Quality Management
• /
• v.41 no.2
• /
• pp.249-260
• /
• 2013

Purpose: The main objective of this paper is to propose an RD method by developing a neural network (NN)-based estimation approach in order to provide an alternative aspect of response surface methodology (RSM). Methods: A specific modeling procedure for integrating NN principles into response function estimations is identified in order to estimate functional relationships between input factors and output responses. Finally, a comparative study based on simulation is performed as verification purposes. Results: This simulation study demonstrates that the proposed NN-based RD method provides better optimal solutions than RSM. Conclusion: The proposed NN-based RD approach can be a potential alternative method to utilize many RD problems in competitive manufacturing nowadays.

• Journal of the Korean Society of Manufacturing Technology Engineers
• /
• v.19 no.1
• /
• pp.114-120
• /
• 2010

Optimization of the turning process has been concentrated on the selection of the optimal cutting parameters, such as cutting speed, feed rate and depth of cut. However, optimization of the cutting parameters does not necessarily guarantee the maximum profit. For the maximization of the profit, parameters other than cutting parameters have to be taken care of. In this study, 8 price-related parameters were considered to maximize the profit of the product. Regression equations obtained from RSM technique to relate the cutting parameters and maximum cutting volume with a given insert were used. The experiments with four combinations of cutting inserts and material were executed to compare the results that made the profit and cutting volume maximized. The results showed that the cutting parameters for volume and profit maximization were totally different. Contrary to our intuition, global optimization was achieved when the number of inserts change was larger than those for volume maximization. It is attributed to the faster cutting velocity, which decreases processing time and increasing the number of tool used and the total tool changing time.

• Journal of Power System Engineering
• /
• v.21 no.3
• /
• pp.85-92
• /
• 2017

Global air traffic is forecast to grow at an average annual rate of around 5% in the next 20 years. The continuous growth of air traffic and raised environmental awareness put increasing pressure on aero engine manufacturers to reduce fuel burn and emissions. NEWAC are a new integrated program of the European Union with focus on innovative core engine concepts to achieve this problem. In this paper, Within NEWAC, active core engine configurations will be investigated. the investigation is focused on the optimal design of the CAC heat exchanger for active core. For optimal design of he CAC heat exchanger, the HTFS of basic design of heat exchanger are analyzed so as to proceed an optimization routines based on Response Surface Method(RSM) and Design of Experiment(DOE). As a result, CAC heat exchanger optimized by 1.0314 lb/s mass flow rate and 3.9058 mm TP of tube layout and 206.8181 mm height of heat exchanger and 918 tube number for heat transfer and pressure drop. We confirm the design optimization using RSM and DOE is useful on complex structure of heat exchanger.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.27 no.1
• /
• pp.144-151
• /
• 2003

In this paper, an optimum design problem for endosseous implant in dentistry is studied to find best implant design. An optimum design problem is formulated to reduce stresses arising at the cortical as well as cancellous bones, in which sufficient design parameters are chosen fur design definition that encompasses major implants in popular use. Optimization at once (OAO) with the large number of design variables, however, causes too costly solution or even failure to converge. A concept of multilevel optimization (MLO) is employed to this end, which is to group the design variables of similar nature, solve the sub-problem of smaller size fur each group in sequence, and this is iterated until convergence. Each sub-problem is solved based on the response surface method (RSM) due to its efficiency for small sized problem. Favorable solution is obtained by the MLO, which is compared to both solutions made by RSM and sequential quadratic programming (SQP) in the OAO problem.