• Molecules and Cells
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• v.41 no.2
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• pp.127-133
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• 2018

Chromatin remodeling factors are involved in many cellular processes such as transcription, replication, and DNA damage response by regulating chromatin structure. As one of chromatin remodeling factors, remodeling and spacing factor 1 (RSF1) is recruited at double strand break (DSB) sites and regulates ataxia telangiectasia mutated (ATM) -dependent checkpoint pathway upon DNA damage for the efficient repair. RSF1 is overexpressed in a variety of cancers, but regulation of RSF1 levels remains largely unknown. Here, we showed that protein levels of RSF1 chromatin remodeler are temporally upregulated in response to different DNA damage agents without changing the RSF1 mRNA level. In the absence of SNF2h, a binding partner of RSF1, the RSF1 protein level was significantly diminished. Intriguingly, the level of RSF1-3SA mutant lacking ATM-mediated phosphorylation sites significantly increased, and upregulation of RSF1 levels under DNA damage was not observed in cells overexpressing ATM kinase. Furthermore, failure in the regulation of RSF1 level caused a significant reduction in DNA repair, whereas reconstitution of RSF1, but not of RSF1-3SA mutants, restored DSB repair. Our findings reveal that temporal regulation of RSF1 levels at its post-translational modification by SNF2h and ATM is essential for efficient DNA repair.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.17 no.6
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• pp.113-119
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• 2017

The Rich Communication Service(RCS) is a next generation mobile messaging service. Since it has been developed and managed by a wireless service provider, 3rd party application developers cannot utilize the RCS features in their apps. A few studies have been proposed to solve this problem, even though they were not viable level of deployment. This paper presents a Web-based Rich Communication Service Software Framework(WebRSF) to help the 3rd party application developers adopt RCS features easily. WebRSF converts complicated RCS protocols to simple web-based protocols for providing the RCS 1-to-1 chat service to the 3rd parties. Communication protocols between WebRSF and its clients are defined in this paper. Also, a network configuration diagram and a message flow diagram are defined. Finally, performance evaluation between the WebRSF client and the RCS client are performed to simulate the load on clients' devices. It shows that the WebRSF client has 17% less loads than the RCS client while initiating sessions, and has 25% more loads after initiating sessions.

• Journal of Sericultural and Entomological Science
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• v.42 no.2
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• pp.114-119
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• 2000

Effects of ethanlo treatment on the structural and thermal characteristics of regenerated Antheraea pernyi silk fibroin (RSF) were investigated. Infrared spectroscopy and X-ray diffractometry showed that the conformational transition of RSF might be affected by concentration of ethanol and its treatment time. The structure of RSF was rapidly changed from random coil to $\beta$-sheet conformation when RSF was treated with les than 75% ethanol concentration. However, RSF treated with ethanol(100%) did not show conformational change. Differential scanning calorimetry showed that exotherm at 232$\^{C}$ disappeared and the intensity of endotherm at 228$\^{C}$ decreased with treatment of 75% ethanol. Dynamic thermal analysis showed that loss modulus (E") and tan $\delta$$\_$E/ of RSF treated with aqueous ethanol was broaden and shifted to higher temperature in comparison with those of untreated RSF.

• Journal of the Korean Wood Science and Technology
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• v.39 no.3
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• pp.221-229
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• 2011

Due to the increase of oil price and the environmental issue such as the emission of volatile organic compounds, the necessity for developing alternative resins of petroleum-based adhesive resins, which have extensively been used for the manufacture of wood-based products, has been speculation since the early 1990. In our study, rapeseed flour (RSF), which is the by-product of bio-diesel produced from rapeseed, were hydrolyzed by enzymes. As a crosslinking agents of the RSF hydrolyzates, phenol-formaldehyde prepolymers (PF) were prepared. The RSF hydrolyzates and PF were mixed to complete the formulation of RSF-based adhesive resins, and the resins were applied to make the laminated veneer lumber (LVL). The physical and mechanical properties of the LVL were measured to examine whether RSF can be used as raw materials of adhesive resins for the fabrication of LVL or not. The average moisture content and soaking delamination rate of the LVL bonded with RSF-based adhesive resins exceeded the minimum requirement of KS standard. Moreover, thermal analysis of the RSF-based resins showed similar tendencies except for the RSF-based adhesive resins formulated with pectinase-hydrolyzed RSF. The bending strengths of the LVL were higher than that of the LVL made with commercial PF resins. These results showed the potential of RSF as a raw material of alternative adhesives for the production of LVL. Further works on the optimal conditions of RSF hydrolysis and spreading characteristics for RSF-based adhesive resins is required to improve the adhesive performance of RSF-based resins.

• Journal of the Korean Wood Science and Technology
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• v.40 no.3
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• pp.177-185
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• 2012

The interest to develop adhesives from renewable resources is growing to substitute petroleum-based adhesive resins in the manufacture of wood based panels. In our study, rapeseed flour (RSF), which is the by-product of bio-diesel produced from rapeseed, were hydrolyzed with acid and alkali. As a crosslinking agents of the RSF hydrolyzates, phenol-formaldehyde prepolymers (PF) were prepared. The RSF hydrolyzates and PF were mixed to complete the formulation of RSF-based adhesive resins, and the resins were applied to make the medium density fiberboard (MDF). The physical and mechanical properties of the MDF were measured to examine whether RSF can be used as raw materials of adhesive resins for the manufacture of MDF or not. The average moisture content and density of the MDF made with RSF-based adhesive resins satisfied the minimum requirement of KS standard, but the thickness swelling was not. The bending strengths of the MDF made with RSF-based adhesive resins were lower than that of the MDF made with commercial UF resins, but the internal bonding strengths of tested MDF in some make-up conditions of RSF-based adhesive resins were higher than that of MDF made with commercial UF resins. These results showed the potential of RSF as a raw material of adhesives for the production of MDF. Future works on the optimal manufacturing process conditions of MDF made with RSF-based adhesive resins are required to improve the performance of MDF made with RSF-based resins.

• Journal of the Korean Society of Food Science and Nutrition
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• v.25 no.6
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• pp.963-968
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• 1996

Effects of raw soy flour(RSF) and RSF with magnesium supplementation(RSF+Mg) feeding on lipid metabolism and serum glucose concentration of diabetic and nondiabetic rats were studied. Male rats(Sprague-Dawley, body weight $332.8\pm19.3g)$ were assigned to four dietary groups and fed for 4 weeks. For each experimental diet some rats were injected with streptozotocin in intraperitonium (i.p.) to induce diabetes, and other rats were injected with buffer i.p. as a nondiabetic group. The diabetic rats fed diets of control, RSP and RSF+Mg showed significant body weight decrease(-67~-79g) compared with nondiabetic group. Pancreas weights in diabetic RSF and RSF + Mg groups were heavier than those of the control and nondiabetic groups. , Fasting serum glucose level of diabetic RSF and RSF+Mg groups significantly decreased by 73 and 44% compared with the diabetic control group, but they significantly increased by 77 and 111% compared with the nondiabetic group. Serum triglyceride, phospholipid and total cholesterol concentration of diabetic RSF and RSF + Mg groups significantly decreased compared with the diabetic control group. Serum HDL-cholesterol, HDL-cholesterolitoal cholesterol ratio and liver total cholesterol were not affected by RSF and RSP+Mg feeding, Liver total lipid and triglyceride concentration were significantly lower in diabetic groups than those in the nondiabetic group. The results indicated that RSF and RSF+Mg feeding did not produce hyperlipemia and somewhat improved the glucose levels in diabetic rats.

• International Journal of Industrial Entomology and Biomaterials
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• v.45 no.2
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• pp.70-77
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• 2022

Wet-spun regenerated silk fibroin (RSF) fibers have been extensively studied owing to their 1) useful properties as biomaterials, including good blood compatibility and cyto-compatibility; 2) the various methods available to control the structural characteristics and morphology of the fiber, and 3) the possibility of fabricating blended fibers and new material-embedded fibers. In this study, silk nanofibrils prepared using a new method were embedded in RSF to fabricate wet-spun silk nanofibril/RSF composite fibers. Up to 2% addition of silk nanofibril, the silk nanofibril/RSF dope solution showed slight shear thinning, and the G' and G" of the dope solution were similar. However, above 3% silk nanofibril content, the viscosity of the dope solution significantly increased. In addition, shear thinning was remarkably evident, and the G' of the dope solution was much higher than the G", indicating a very elastic state. As the silk nanofibril content was increased, the wet-spun silk nanofibril/RSF composite fiber became uneven, with a rough surface, and more beaded fibers were produced. Scanning electron microscopy observations revealed that the beaded fibers were attributed to the inhomogeneous dispersion and presence of agglomerates of the silk nanofibrils. As the silk nanofibril content and RSF concentration increased, the maximum draw ratio decreased, indicating the deterioration of the wet spinnability and post-drawing performance of silk nanofibril/RSF.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.9
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• pp.1426-1432
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• 2013

Changes in the textural properties of rice cookies as a result of using roasted soybean flour (RSF) as an additive were investigated in this study. RSF was replaced as 3, 6, 9, 12, 15 and 20% of 100 g of rice flour. The addition of RSF increased the hardness of dough and cookies as measured by texture analyzer (TA), whereas moisture content of dough and cookies decreased upon addition of RSF. Further, the addition of RSF led to an increase in the L value of dough and cookies, but to a decrease in the a and b values. In addition, the bulk density of cookies decreased upon addition of RSF, whereas bulk density of dough also declined when compared to control. The spreadability of cookies was found to be lower in comparison to control. The values of ${\Delta}bulk$ densities (BD) and ${\Delta}hardness$ of cookies also increased upon addition of RSF. From the point of view of sensory evaluation, addition of 9% RSF to rice cookies showed the highest score in terms of overall acceptability and softness. A 12% RSF addition to rice cookies led to the highest score in sandiness. As a result, we conclude that the crispy texture of the rice cookie can be improved by adding RSF.

• Journal of the Korea institute for structural maintenance and inspection
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• v.13 no.1 s.53
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• pp.169-178
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• 2009

Ring-type steel fibers (RSFs) of the closed circular shape, have different resistance mechanisms other than straight steel fibers. RSFs also maintain the same value of the orientation factor for the plane enclosed by the fiber ring perimeter. In this research, comparative studies were performed for the panels reinforced with RSFs and with straight steel fibers of $15kg/m^3$ and $30kg/m^3$, respectively. Resisting mechanisms of RSFs were identified and higher toughness indices were experimentally observed for the concrete panels reinforced with RSFs than with straight steel fibers. Orientation factor for the RSF was suggested. It was found that RSFs were more effective in increasing toughness for the panel specimens with relatively thinner thickness than beam specimens.

• Proceedings of the Korean Vacuum Society Conference
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• 1999.07a
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• pp.192-192
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• 1999

SIMS(Secondary Ion Mass Spectrometry)는 다른 표면 분석장비와 비교하여^g , pp m,^g , pp b 단위의 미량분석이 가능한 장비로서, 특히 depth Profiling을 위한 dynamic SIMS는 Mass Spectrometer의 종류에 따라 Quadrupole SIMS (Q-SIMS)와, Magnetic Sector SIMS (M-SIMS)로 분류된다. 한편, Q-SIMS와 달리 M-SIMS의 경우, Transmission을 높여 주기 위해 Sample Holder에 수 keV의 bias를 걸어 주는데, 이로 인하여 분석 원소에 대한 Sensitivity가 향상되어 지는 반면, RSF의 변화와 같은 분석상의 Artifact가 발생하게 된다. 일반적으로 Q-SIMS의 경우에는 RSF의 RSD(Relative Standard Deviation)가 1%이내에서 보고되고 있지만 M-SIMS에 있어서는 이러한 Deviation이 M-SIMS보다 크게 나타난다. 이 차이는 주로 Sample Holder와 Immersion Lens 사이에 형성되는 Magnetic Field의 왜곡과 Spectrometer의 문제로부터 발생한다. 본 논문에서는 Sample Holder의 종류 및 holder so window 위치에 따라 RSF의 차이를 측정하고 그 data를 RS/1 통계 Package를 이용하여 계량적으로 검증하였으며, 그 차이의 원인과 대책을 제시하고자 한다. 실험에 사용된 Sample은 Si(100) p-type Wafer에 Boron을 이온 주입하여 제작하였다. 이온 주입 장비는 Varian E-500HP이며, 5.0E13 ions/cm2의 dose양을 80keV의 Energy로 각각 7도와 22도의 Tilt와 Twist Angle로 이온 주입을 하였다. SIMS분석에 사용된 Sample Holder는 각각 3 Hole, 9 Hole Type HOlder이며, 분석은 Cameca IMS-6f를 사용하여 B에 대한 Matrix Peak으로 28Si++를 얻었다. 실험 결과 3 Hole Type Sample Holder의 경우 RSF의 RSD는 5.84%, 9Hle Type Sample Holder의 경우는 14.3%로 나타났으나 분석 Window의 위치에 따르는 Grouping을 실시한 결과, 3 Hole Type Sample Holder의 경우 1.2%, 9Hole Type Sample Holder의 경우 9.8%로 RSF의 변화가 감소하였다. 이러한 Deviation은 Sample Holder를 Mount시킬 때 세 개의 Screw를 이용하여 Immersion Lens와의 평형을 잡아주기 때문에 발생하며, 이 Munting을 정확히 해줌으로써 RSF의 변화를 줄일 수 있으나, 실제로 완벽한 Mounting이 불가능하기 때문에 RSF를 일정하게 하기 위해서는 Sample Holder so Window의 취치를 일정하게 설정한 후 분석을 실시해야 한다고 판단된다.