• Proceedings of the Botanical Society of Korea Conference
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• 1985.08b
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• pp.51-57
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• 1985

In order to evlauate feasibility of the gene tagging by the maize transposable element Ac in heterologous plant systems, we have investigated physical distances and directions of transposition of the element in Arabidopsis thaliana and tobacco cultured cell line BY-2. We prepared a T-DNA construct that carried a non-autonomous derivative of Ac with a site for cleavage by endonuclease I-Scel (designated dAc-I-RS element). Another cleavage site was also introduced into the T-DNA region outside dAc-I-RS. A number of transgenic Arabidopsis plants were generated, each of which had a single copy of the T-DNA at a different chromosomal location. To examine the pattern of transposition, three out of these transgenic plants were crossed with the Arabidopsis plant that carried the gene for Ac transposase and progeny in which dAc-I-RS had been transposed were isolated. After digestion of the genomic DNA of these progeny with I-SceI, sizes of segment of DNA were determined byd pulse-field gel electrophoresis. We also performed linkage analysis for the transposed elements and sites of mutations near the elements. Our results with three transgenic lines showed that 50% of all transposition events had occurred within 1, 700 kilo-base pairs (kb) on the same chromosome, with 35% within 200 kb, and that the elements transposed in both directions on the chromosome with roughly equal probability. The data thus indicate that the Ac-Ds system is most useful for tagging of genes that are present within 200 kb of the chromosomal site of Ac in Arabidopsis. In addition, determination of the precise localization of the transposed dAc-I-RS element should definitely assist in map-based cloning of genes around insertion sites. In the present paper, we report typical examples of such gene isolation studies.

• Journal of Korean Physical Therapy Science
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• v.18 no.2
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• pp.73-80
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• 2011

Purpose: The purpose of this study was to investigate the correlation between the visual perception ability and the static dynamic balance ability in health elderly. Method: The Motor Free Visual Perception Test-Row Score(MVPT-RS) and MVPT-Process Time(MVPT-PT) were used for evaluating the visual perception abilities. Assessment of the balance ability was taken by using Good Balance System. In the assessment using Good Balance System, X, Y coordinate speed, anterior-posterior direction, medial-lateral direction and Velocity Movement(VM) in standing posture when eye open were measured as static balance abilities. Thirty-seven healthy elderly who live in Gwangyang participated in the experiment for 2 months, from October to November 2010. Results: 1. There were statistically significant differences of MVPT-RS, MVPT-PT, NSB-X, NSB-Y, NSB-VM, OLB-X, and OLB-VM based on the gender(p<0.05). 2. The negative correlations of MVPT-RS:NSB-Y(r=-0.354), MVPT-RS:OLB-X(r=-0.4), MVPT-RS: OLB-Y(r=-0.371), but positive correlations of MVPT-PT:DTB-T showed a statistical significance(r=0.45, p<0.05). 3. The positive correlations of NSB-X:NSB-Y(r=0.54), NSB-X: NSB-VM(r=0.848), NSB-Y:NSB-VM(r=0.531), OLB-X:OLB-Y(r=0.876), OLB-X:OLB-VM(r=0.872), and OLB-Y:OLB-VM(r=0.787) showed statistical significances(p<0.05). Conclusion: These results showed that the visual perception ability was correlated with some balance ability in health elderly. Especially the perception test process time(MVPT-PT) has closely related with the DTB-T. The visual perception ability is considered as a factor on the balance ability in health elderly. Further study will focus on the development of improving program of visual perception ability as an improving method of balancing ability in health elderly.

• Genomics & Informatics
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• v.19 no.3
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• pp.29.1-29.10
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• 2021

In our previous studies, we have demonstrated the association of certain variants of the thyroid-stimulating hormone receptor (TSHR), thyroid peroxidase (TPO), and thyroglobulin (TG) genes with congenital hypothyroidism. Herein, we explored the mechanistic basis for this association using different in silico tools. The mRNA 3'-untranslated region (3'-UTR) plays key roles in gene expression at the post-transcriptional level. In TSHR variants (rs2268477, rs7144481, and rs17630128), the binding affinity of microRNAs (miRs) (hsa-miR-154-5p, hsa-miR-376a-2-5p, hsa-miR-3935, hsa-miR-4280, and hsa-miR-6858-3p) to the 3'-UTR is disrupted, affecting post-transcriptional gene regulation. TPO and TG are the two key proteins necessary for the biosynthesis of thyroid hormones in the presence of iodide and H₂O₂. Reduced stability of these proteins leads to aberrant biosynthesis of thyroid hormones. Compared to the wild-type TPO protein, the p.S398T variant was found to exhibit less stability and significant rearrangements of intra-atomic bonds affecting the stoichiometry and substrate binding (binding energies, ΔG of wild-type vs. mutant: -15 vs. -13.8 kcal/mol; and dissociation constant, K_d of wild-type vs. mutant: 7.2E^-12 vs. 7.0E^-11 M). The missense mutations p.G653D and p.R1999W on the TG protein showed altered ΔG(0.24 kcal/mol and 0.79 kcal/mol, respectively). In conclusion, an in silico analysis of TSHR genetic variants in the 3'-UTR showed that they alter the binding affinities of different miRs. The TPO protein structure and mutant protein complex (p.S398T) are less stable, with potentially deleterious effects. A structural and energy analysis showed that TG mutations (p.G653D and p.R1999W) reduce the stability of the TG protein and affect its structure-functional relationship.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.17
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• pp.7213-7218
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• 2014

Background: Egypt has one of the highest prevalences of hepatitis C virus (HCV) infection worldwide. Although the IL28B gene polymorphism has been shown to modify the course of chronic HCV infection, this has not been properly assessed in the Egyptian population. Materials and Methods: The IL28B rs12979860 single nucleotide polymorphism (SNP) was therefore examined in 256 HCV-infected Egyptian patients (group II) at different stages of disease progression and in 48 healthy volunteers (group I). Group II was subdivided into GII-A (chronic hepatitis patients, n=119), GII-B (post hepatitis cirrhosis, n=66) and GII-C (HCC on top of cirrhosis, n=71). Results: The C/T genotype was the commonest in all groups. It was more frequent in GI (52%) than in GII (48%). There was no significant difference in the frequency of C/T and C/C or T/T genotypes between groups and subgroups (p=0.82). Within the subgroups; the C/C genotype was more common in GII-B while C/T and T/T genotypes were more common in GII-C, though with no significant difference (p=0.59 and p=0.80). There was no significant association between IL28B rs12979860 SNP and viral load, ALT, AFP level, METAVIR scores for necro-inflammation and fibrosis, and Child-Pugh classification. Conclusions: 1) IL28Brs12979860 C/T genotype is the commonest genotype in HCV-associated CH and HCC in Egypt. 2) IL28Brs12979860 polymorphisms are not associated with disease progression or aggression (histological staging, severity of fibrosis in CH or the incidence of post-HCV HCC). 3) Differences in IL28Brs12979860 genotypes could be a consequence of environmental or ethnic variation.

• Korean Journal of Medicinal Crop Science
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• v.12 no.2
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• pp.171-178
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• 2004

The objectives of this study were to establish the genetic transformation system of stilbene synthase in Rehmannia glutinosa. Resveratrol, which is both a phytoalexin with antifungal activity and a phytochemical associated with reduced cancer risk and reduced cardiovascular disease, is synthesized in a limited number of plant species including peanut. Resveratrol synthesis is catalyzed by the enzyme stilbene synthase including resveratrol synthase (RS). Stilbene synthase gene (RS3) obtained from peanut, Arachis hypogaea, Fabaceae has been transferred into chinese foxglove, Rehmannia glutinosa by using Agrobacterium mediated transformation. PCR analysis with RS3 primer confirmed that the targeted gene was introduced into the plant genome, 904 bp in size. Further analyses of identification of transformation using developed other molecular techniques and transgenic plants that RS t-DNA introduced to chinese foxglove (R. glutinosa L) and its reaction product, stilbene such as resveratrol will be isolate and characterize using NMR, MS, and HPLC.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.2
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• pp.205-208
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• 2002

The use of chopped sugarcane stalk (CSS) as a roughage for lactating cows was examined using four Holstein crossbred cows in a private dairy farm in Khon Kaen, Thailand, in comparison with rice straw (RS), which is the conventional roughage in the dry season in the region. Cows were subjected to the following two dietary treatments: Diet 1) RS with commercial concentrate feed, and Diet 2) CSS and RS with commercial concentrate feed. The diet was switched over every 3 weeks. The amount of concentrate was determined by the experience of the owner of the cows. RS and CSS were given ad libitum. There was no difference in milk production between two groups, although the total DMI was less in cows fed CSS. Solid-not-fat (SNF) content in milk was significantly higher in the cows given CSS. The NEFA content was significantly lower in the animals given CSS, which suggested that cows given only RS as roughage would be suffering from energy malnutrition. Therefore, it was considered that CSS feeding improved energy supply, which resulted in higher SNF in milk. In the nutritional point of view, the present study clearly showed CSS can be used as a roughage for dairy cows in the dry season.

• Bulletin of the Korean Mathematical Society
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• v.57 no.1
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• pp.69-79
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• 2020

In this paper, we study the properties of T satisfying [CTC, T] = 0 for some conjugation C where [R, S] := RS - SR. In particular, we show that if T is normal, then [CTC, C] = 0. Moreover, the class of operators T satisfy [CTC, T] = 0 is norm closed. Finally, we prove that if T is complex symmetric, then T is binormal if and only if [C|T|C, |T|] = 0.

• Bulletin of the Korean Chemical Society
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• v.31 no.3
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• pp.606-610
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• 2010

Aminoacyl-tRNA synthetases (aaRSs) play vital roles in protein biosynthesis of living organisms and are interesting antibacterial drug targets. In order to find out new inhibitor candidate molecules as antibacterial agent, the binding modes of the candidate molecules were investigated at the active sites of aaRSs by molecular docking study. The docking simulations were performed with 48 compounds from four different scaffolds into the eight different aaRSs. The results show that scaffolds 3 and 4 compounds have consistently better binding capabilities, specifically for HisRS (E. coli) and IleRS (S. aureus). The binding modes of the best compounds with the proteins were well compatible with those of two ligands in crystal structures. Therefore, we expect that the final compounds we present may have reasonable aaRS inhibitory activity.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.3
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• pp.204-208
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• 1991

Five models for net radiation estimation reported by Linacre(1968), Berljand(1956), Nakayama et al. (1983), Chang (1970) and Doorenbos et al. (1977) were tested for the adaptability to Korea. A new model with effective longwave radiation term parameterized by air temperature, solar radiation and vapor pressure was formulated and tested for its accuracy. Above five models with original parameter values showed large absolute mean deviations ranging from 0.86 to 1.64 MJ/$m^2$/day. The parameters of the above five models were reestimated by using net radiation and meteorological elements measured in Suwon, Korea. These five models with new parameter values showed absolute mean deviations ranging from 0.74 to 0.88 MJ/$m^2$/day. The following model was newly formulated: Rn=(1- $\alpha$) Rs- $\sigma$ $T_{k}$$^{4}$ (0.0103 Exp (0 .0731 Rs) -0.0475 (equation omitted) +0 .2478) ($R^2$=0.997, n=63) where $\alpha$ =albedo, $\sigma$=Stefan-Boltzmann constant, Rs=solar radiation in MJ/$m^2$/day, Tk =air temperature in Kelvin and $e_{a}$=vapor pressure in mb. This model revealed 0.4988 MJ/$m^2$/day in absolute mean deviation when applied to an independent set of meteorological data.a.a.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.5
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• pp.2101-2107
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• 2014

Background: Single nucleotide polymorphisms (SNPs) affecting microRNA (miR) sequences may influence carcinogenesis. Our current study primarily aimed to confirm previously conducted association studies between rs2910164 found on miR-146a, and rs11614913 located on miR-196a2 polymorphisms and cancer phenotypes in the Japanese elderly population. rs2910164 (G/C) and rs11614913 (T/C) polymorphisms were determined by genotyping on the samples collected from 1,351 consecutive autopsy cases registered in the Japanese SNPs for geriatric research (JG-SNP) data base. Cancer samples were systematically reviewed, pathologically verified and assessed with respect to miR-146a and miR-196a2 genotypic variation. The current study covered 726 males and 625 females with a mean age of $80.3{\pm}8.9$ years. The study included 524 subjects without cancer and 827 subjects with at least one type of cancer, such as gastric (n=160), lung (n=148), colorectal (n=116) or others. Males with cancers (n=467) were more numerous than females (n=360). Both rs11614913 (CT: TT adjusted odds ratio (OR) 95% confidence interval (95%CI)=0.98 (0.75-1.28), p=0.873, CC: TT adjusted OR (95%CI)=1.06 (0.76-1.47), p=0.737, CT+CC: TT, adjusted OR (95%CI)=0.99 (0.77-1.29), p=0.990), and rs2910164 (CG: CC adjusted OR (95%CI)=1.12 (0.87-1.44), p=0.383, GG: CC adjusted OR (95%CI)=1.03 (0.71-1.48), p=0.887, CG+GG: CC adjusted OR (95%CI)=1.10 (0.87-1.39), p=0.446) polymorphisms did not show significant association with overall cancer in all subjects. However, "CC" genotype in rs11614913 polymorphism was significantly associated with increased gastric cancer (n=160) in all subjects (CC: CT+TT, adjusted OR (95%CI)=1.50 (1.02-2.22), p=0.040). We found that rs11614913 and rs2910164 do not pose general cancer risk, but rs11614913 may influence gastric cancer in Japanese elderly population. Confirmation of our study results requires further investigations with larger subject populations.