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Evaluation of waterlogging tolerance using chlorophyll fluorescence reaction in the seedlings of Korean ginseng (Panax ginseng C. A. Meyer) accessions (엽록소 형광반응을 이용한 인삼 유전자원의 습해 스트레스 평가)

  • Jee, Moo Geun;Hong, Young Ki;Kim, Sun Ick;Park, Yong Chan;Lee, Ka Soon;Jang, Won Suk;Kwon, A Reum;Seong, Bong Jae;Kim, Me-Sun;Cho, Yong-Gu
    • Journal of Plant Biotechnology
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    • v.49 no.3
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    • pp.240-249
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    • 2022
  • Measuring chlorophyll fluorescence (CF) is a useful tool for assessing a plant's ability to tolerate abiotic stresses such as drought, waterlogging and high temperature. Korean ginseng is highly sensitive to water stress in paddy fields. To evaluate the possibility of non-destructively diagnosing waterlogging stress using chlorophyll fluorescence (CF) imaging techniques, we screened 57 ginseng accessions for waterlogging tolerance. To evaluate waterlogging tolerance among the 2-year-old Korean ginseng accessions, we treated ginseng plants with water stress for 25 days. The physiological disorder rate was characterized through visual assessment (an assigned score of 0-5). The physiological disorder rates of Geumjin, Geumsun and GS00-58 were lower than that of other accessions. In contrast, lines GS97-62, GS97-69 and GS98-1-5 were deemed susceptible. Root traits, chlorophyll content and the reduction rates decreased in most ginseng accessions. Further, these metrics were significantly lower in susceptible genotypes compared to resistant ones. All CF parameters showed a positive or negative response to waterlogging stress, and this response continuously increased over the treatment time among the genotypes. The CF parameter Fv/Fm was used to screen the 57 accessions, and the results showed clear differences in Fv/Fm between the susceptible and resistant genotypes. Susceptible genotypes had an especially low Fv/Fm value of less than 0.8, reflecting damage to the reaction center of photosystem II. It is concluded that Fv/Fm can be used as a CF parameter index for screening waterlogging stress tolerance in ginseng genotypes.

Evaluation of Temperature and Precipitation over CORDEX-EA Phase 2 Domain using Regional Climate Model HadGEM3-RA (HadGEM3-RA 지역기후모델을 이용한 CORDEX 동아시아 2단계 지역의 기온과 강수 모의 평가)

  • Byon, Jae-Young;Kim, Tae-Jun;Kim, Jin-Uk;Kim, Do-Hyun
    • Journal of the Korean earth science society
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    • v.43 no.3
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    • pp.367-385
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    • 2022
  • This study evaluates the temperature and precipitation results in East Asia simulated from the Hadley Centre Global Environmental Model version 3 regional climate model (HadGEM3-RA) developed by the UK Met Office. The HadGEM3-RA is conducted in the Coordinated Regional climate Downscaling Experiment-East Asia (CORDEX-EA) Phase II domain for 15 year (2000-2014). The spatial distribution of rainbands produced from the HadGEM3-RA by the summer monsoon is in good agreement with the Asian Precipitation Highly Resolved Observational Data Integration Towards Evaluation of water resources (APRODITE) data over the East Asia. But, precipitation amount is overestimated in Southeast Asia and underestimated over the Korean Peninsula. In particular, the simulated summer rainfall and APRODITE data show the least correlation coefficient and the maximum value of root mean square error in South Korea. Prediction of temperature in Southeast Asia shows underestimation with a maximum error during winter season, while it appears the largest underestimation in South Korea during spring season. In order to evaluate local predictability, the time series of temperature and precipitation compared to the ASOS data of the Seoul Meteorological Station is similar to the spatial average verification results in which the summer precipitation and winter temperature underestimate. Especially, the underestimation of the rainfall increases when the amounts of precipitation increase in summer. The winter temperature tends to underestimate at low temperature, while it overestimates at high temperature. The results of the extreme climate index comparison show that heat wave is overestimated and heavy rainfall is underestimated. The HadGEM3-RA simulated with a horizontal resolution of 25 km shows limitations in the prediction of mesoscale convective system and topographic precipitation. This study indicates that improvement of initial data, horizontal resolution, and physical process are necessary to improve predictability of regional climate model.

Effects of Coir Substrate Application and Substrate Volume on the Growth and Yields of Strawberry in a Hydroponically Cultured System (딸기 수경재배에 코이어 배지 적용과 근권부 배지 용량이 생육 및 수확량에 미치는 영향)

  • Hwang, Jeongsu;Yun, Sungwook;Kwon, Jinkyung;Park, Minjung;Lee, Dongsoo;Lee, Heeju;Lee, Siyoung;Lee, Sanggyu;Hong, Youngsin
    • Journal of Bio-Environment Control
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    • v.31 no.3
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    • pp.163-169
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    • 2022
  • This study was conducted to examine an alternative cultivating method that uses coir substrates in a hydroponically cultured system. Three treatment conditions were applied with one-layer substrate (10 cm height) with a coir chip and dust ratio of 5:5 (Treatment A), two-layer coir substrate (20 cm height) with a coir chip and dust ratio of 5:5 (Treatment B), one-layer coir substrate (15 cm height) with a coir chip and dust ratio of 7:3 (Treatment C). The control condition was a plastic container filled with a coir chip and dust ratio of 5:5. Various criteria were measured and compared between the treatments and the control. The yield of strawberry was smaller in the control than in the treatments. No significant difference in growth characteristic was found in the height treatments of the coir substrates. The net photosynthetic rate of the treatments was 14.68-15.76 µmol CO2·m-2·s-1. This does not show a statistically significant difference. The root activity was better in treatment B and C than in treatment A and the control. The length and width of leaves were measured as 4.04-4.13 cm and 3.26-3.34 cm. These results are not statistically significant. The leaf length and width ratio was 1.27 in the control and 1.24 in the treatments. The findings show that no statistically significant benefit was found when utilizing coir substrates with different height treatments in the hydroponic culture system. However, the harvested fruit per plant weights 72.38 g in treatment A and 48.69 g in treatment C. The number of harvested fruit was least in treatment C in which a coir chip and dust ratio of 7:3 was applied. Therefore, further research is needed to examine how the chip and dust ratio in coir substrate affects growth characteristics.

Growth Characteristics of Strawberry Runner Plants according to Mixing Ratio of Reused Rockwool, Decomposed Granite, and Horticultural Media (재사용 암면, 마사토 및 원예용 상토의 혼합비율에 따른 딸기 자묘의 생육 특성)

  • Jeong, Ji-Hee;Bae, Hyo Jun;Ko, Baul;Ku, Yang Gyu;Kim, Ho Cheol;Bae, Jong Hyang
    • Journal of Bio-Environment Control
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    • v.31 no.4
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    • pp.497-503
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    • 2022
  • This study was conducted to investigate the horticultural media + decomposed granite + reused rock wool in the following mixing ratio: Control = 100:0, M1 = 80:0:20, M2 = 60:30:10, M3 = 40:30:30, M4 = 30:40:30, M5 = 0:50:50 (reused rockwool : decomposed granite : horticultural media) and develop the physicochemical properties and the growth of 'Sulhyang' strawberry runner plant. In the physical aspect of the horticultural media, statistical differences were recognized that the bulk density and particle density were lower in the control and M1. But the bulk density and particle density were high in the M3, M4, and M5, because it had high mixing ratio between recycled rock wool and decomposed granite. EAW and WBC showed a similar tendency. The air porosity and total porosity were higher in control and M1 than M3, M4, M5. Exchangeable cation (K+, Ca2+, Na+, Mg2+) and base replacement capacity (CEC) were higher in control and M1, than M2, M3, M4, and M5. As a result of the cultivation of 'Sulhyang' runner plant, the plant length was long in M2, 32.1 cm and smaller than M5 to 28.4 cm. However, if the crown diameter, which is the growth indicator of the runner plant, all 6 treatments were formed 11.23 mm-12.03 mm, which is considered to be suitable for the growth of the runner plant. There wasn't a statistical difference between the weight and dry weight of the root. As a result, the growth difference of the seedlings by the horticulture media was similar. Therefore, considering the physical properties of the horticultural media, it was judged that the air porosity and total porosity would be improved when the recycled rock wool and the decomposed granite were properly mixed rather than the use of the horticultural media as a single medium, which would be advantageous for irrigation management.

Early Growth Characteristics of Quercus rubra Associated with Soil Physicochemical Properties and Meteorological Factors in Six Regions of South Korea (토양 물리·화학적 성질 및 기상인자에 따른 국내 6개 지역의 루브라참나무 초기 생장 특성)

  • Hwang, Hwan Su;Kim, Tae Lim;Oh, Changyoung;Lim, Hyemin;Lee, Il Hwan
    • Journal of Korean Society of Forest Science
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    • v.111 no.3
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    • pp.357-364
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    • 2022
  • We investigated the early growth characteristics of Quercus rubra planted in six regions (Hwaseong, Yangpyeong, Pyeongchang, Samcheok, Chungju, and Gimje) in South Korea in relation to soil physicochemical properties and meteorological factors. Q. rubra (1-0) were planted at a density of 3,000 trees ha-1. The average height, root collar diameter (RCD), and volume of 8-year-old Q. rubra planted in 2014 were 3.52 m, 3.84 cm, and 0.0023 m3, respectively. The growth parameters of Q. rubra were the highest and lowest in Hwaseong and Pyeongchang, respectively. Correlation analysis among the soil physicochemical properties, meteorological factors, and plantation growth characteristics found that pH was the only soil factor negatively correlated with RCD, and the other soil factors were not significantly correlated with the growth characteristics. However, growth characteristics were positively correlated to average temperature from March to October and daily maximum temperature; and they were negatively correlated to altitude, topology, and the number of rainy days from March to October. In particular, the trees planted in Hwaseong area showed the best early growth characteristics because this area had the highest daily maximum temperature, the x average temperature from March to October, the low altitude, and it is located close to the foot of a mountain. In Pyeongchang, the early growth characteristics were negatively affected by winter cold damage because of the high altitude, low daily minimum temperature, and damage by wild animals. In Hwaseong, meteorological factors such as temperature and altitude were more highly correlated to growth characteristics of Q. rubra than the physicochemical soil properties. These results will provide useful information for determining suitable sites for Q. rubra plantations and for predicting early growth characteristics in response to environmental factors.

Efficacy of Three Antiviral Agents and Resistant Cultivars on Tomato Yellow Leaf Curl Virus in Tomato (토마토황화잎말림바이러스병에 대한 저항성 품종과 항바이러스 활성 물질 3종의 효과 검증)

  • Kwon, Yongnam;Cha, Byeongjin;Kim, Mikyeong
    • Research in Plant Disease
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    • v.28 no.2
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    • pp.82-91
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    • 2022
  • Recently, several in vitro studies have reported antiviral activity of agents of systemic acquired resistance against plant virus infection, but the approach has not been applied in a wide range of agricultural fields. The objective of this study was to evaluate the inhibitory effect of the exogenous application of salicylic acid (SA), chitosan (CH), or eugenol (EG) in tomato yellow leaf curl virus (TYLCV) infection of greenhouse-grown tomato plants. In vitro, the initial time of symptom was observed in TYLCV-infected plants (VP) of the resistant cultivar 'Superdotaerang' at 12 days post inoculation (dpi) after application of antiviral agents. At 32 dpi, the disease rate of TYLCV in the CHT+VP (0.1% chitosan and virus infected control) treated plants was 87.5%, lower than that of the other treatment. However, the virus content in the CHT+VP treated plants was higher than those of the other treatments, and SA, EG, and CH did not show significant effect on plant height or shoot and root fresh weight. Our results from summer-cultivated greenhouse-grown tomatoes show that none of the tested agents had an inhibitory activity on viral infection or yield of tomato 'Dotaerangsola'cultivar. In contrast, all treated 'TY Giants' cultivars that possessed TYLCV resistance genes Ty-1 and Ty-3a did not show typical symptoms and the virus content was remarkably lower than those in the TYLCV treated plants in 'Superdotaerang'. The results of this research indicated that the planting of resistant tomato cultivars was effective method instead of using SA, EG, and CH (known as resistance-inducing factors for control) of TYLCV in the field.

Effects of Long Term Fertilizations on Growth, Yield and Grain Development of Rice (비료의 장기연용이 벼의 생육ㆍ수량 및 미립발달에 미치는 영향)

  • Han, Hee-Suk;Lee, Moon-Hee;Shim, Jai-Sung
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.36 no.1
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    • pp.41-51
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    • 1991
  • This study was conducted to determine the effect of 20 years long term fertilizations on the physical and chemical properties of paddy soil and the growth, yield, yield components and grain development of rice. Non-fertilized, PK, NK, NP, NPK, NPK + compost, NPK+straw and NPK+lime have been applied since 1968 after surface paddy soil was removed. NPK+compost and NPK+straw applications increased the content of organic matter, available P and CEC, and lime increased soil acidity and SiO$_2$ content. While chemical contents in non-fertilized treatment were low as compared with other treatments. Soil porosity was higher in NPK+straw (51.4%) and NPK+lime(53.1%) than in NPK application (49.8%). Soil hardness was highest in the NPK application and was lowest in the NPK + lime. Continuous application of straw with NPK markedly increased the content of aggregate with over 1mm(19.6%) as compared with NPK application (7.1%). Plant height, tiller number, root number, leaf area index and total dry weight were higher in the applications of compost, straw and lime with NPK than in any other treatments. Brown rice yield in non-fertilized, PK and NP applications was decreased 45, 55, 15 and 5% of that in NPK application, respectively, while application of compost, straw and lime with NPK increased the yield by 11, 14 and 4%, respectively, during 20 years. The number of differentiated rachis branchs in the application of compost, straw and lime was 17 to 21 and that in the other application was 13 to 15, whereas the degenerated rachis branchs was low in the application of compost, straw and lime with NPK. The applications having higher level of perfect rice grain such as non-fertilized, NPK+compost, NPK+straw and NPK+lime had high grain weight and had low level of white core rice, white belly rice. The white core and belly rice was highest in the NP application and notched belly rice kernel was markedly increased in NK and NP applications. The period of grain filling was 30 DAH at NP and NPK applications, 35 DAH at NK and NPK+lime, 40DAH at NPK+compost and NPK+ straw, and 45DAH at non-fertilized, respectively.

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Transition of Rice Culture Practices during Chosun Dynasty through Old References V. Cultivation and Cropping Patterns (주요 고농서를 통한 조선시대의 도작기술 전개 과정 연구 V. 재배양식)

  • Lee, Sung-Kyum;Guh, Ja-Ok;Lee, Eun-Woong;Lee, Hong-Suk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.1
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    • pp.104-115
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    • 1992
  • The rice culture techniques included 'Jodosukyungbeob'(旱稻水耕法 : culture techniques of early-ripening paddy rice), 'Mandosukyungbeob' (晩稻水耕法) : culture techniques of late-Ripening paddy rice 'Handobeob'(旱稻<山稻>法 : culture techniques of upland rice), 'Myojongbeob'(苗種法 : culture techniques of paddy rice by transplanting), 'Kunangbeob'(乾秧法 : culture techniques of rice by transplanting which rears seeding in dry paddy) and 'Sudogunpanongbeob'(水稻乾播農法 : culture techniques of paddy rice seeding in dry field). Especially, 'Kunangbeob' and 'Sudogunpanongbeob' were originally developed in Korea as seen in 1600s(Kyoungje : 經濟) and early 1800s (Yoji : 要旨). In 'Jodosukyungbeob' it took 9 days for seed dipping, water-sprouting and prevent damage by birds, each for 3 days in China, but in Korea seed dipping in water took 3 days and the rest of the procedures were flexibly established. In matured soils, practices were fall plowing right after harvest, recognition of effective tillering and additional fertilization use of human manure, and stimulation of sprouting by lime application. The unique culture techniques adequate for Korean situations were practiced, which included weed control after draining accurately for 3 to 4 times, draining at mid season for improving wind and drought tolerance, rice harvesting at appropriate time for preventing grain shattering, and seeding in rows. 'Mandosukyungbeob' was improved techniques contrast to those of China, and the major contents were selection of proper varieties, good stand establishment by seeding high rates, induction of vigorous tillers, and adoption of 'Jokjongbeob'(足種法 : seeding method by foot). Also, one of the most prominent rice cultures by our ancestors was 'Kunpanongbeob' that was systemized form habitual practice of Pyongan Province. The unique technique actualized was 'Hando [旱稻(山稻)]' culture technique which was the combinations of 'Jokjongbeob', root stimulation method, and disaster-tolerant mixture cropping with adoptation of variety theory, although it was originated from China. The transplanting techniques has come before 'Jikseol'($\ulcorner$直說$\lrcorner$) and its merits were sufficiently realized. However, this method was basically prohibited from the early Chosun dynasty because extremely bad harvest was expected under drought conditions and insufficient conditions of water storage. But, it was permitted in the areas that contained water all the times and in case of large-scale farming especially. Most of rice culture was transplanted in the end of the Chosun dynasty because transplanting was continuously spreaded in the three southern provinces of Korea. Under these circumstances, transplanting technique was improved from the early to the end of the Chosun dynasty by weed control, fertilizing, water management, and quadratic transplanting. Based on these techniques, agricultural productivity was improved 5 times by that time. 'Kunpanongbeob' was created and developed properly for Korean conditions that is dry in early season and flooding in late season. This was successively developed and established into transplanting technique of nursery seedling.

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Effects of Nitrogen Fertilization Rate at Different Nursery Soils on Seedling Characters and Endosperm Consumption in Rice Seedling (상토 종류별 질소시비량이 벼 어린모 묘소질 및 배아양분 소모에 미치는 영향)

  • Kim, Sang-Su;Choi, Min-Gue;Lee, Seong-Yong;Yoo, Chul-Hyun;Cho, Soo-Yeon;Jun, Byung-Tae
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.5
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    • pp.514-520
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    • 1996
  • To clarify the proper nitrogen application level for rasing rice infant seedling under different nursery soil, Dongjinbyeo was raised at seedling box with different basal nitrogen level. The results are as follows. The higher the nitrogen level was, the lower emergence rate and the lower establishment rate. The establishment rate was less than 90%, when the nitrogen was more than 2g/box in hill soil and more than 1g/box in paddy soil. The more the nitrogen level was, the higher the seedling height in hill soil, but was higher in the order of N-2, 3, 1 and 0g /box. Leaf number wasn't significantly different between nusery soils and among nitrogen levels when seedling was raised more than 6 days. The endosperm survival rate was decreased as the increased nitrogen level, but wasn't different between the nursery soils. Amount of root was decreased as the increased nitrogen level in paddy soil, but was heavier in the order of N-1, 0, 2 and 3g /box in hill soil. Mat formation was better as the nitrogen level was decreased in all nursery soils. Considering the emergence rate, seedling charactors and mat formation, the proper nitrogen levels seemed to be 2g /box for hill soil and 1g /box for paddy soil.

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Thermal Behavior and Leaf Temperature in a High Pressure Sodium Lamp Supplemented Greenhouse (고압나트륨등 보광 온실의 열적 거동 및 엽온 분석)

  • Seungri Yoon;Jin Hyun Kim;Minju Shin;Dongpil Kim;Ji Wong Bang;Ho Jeong Jeong;Tae In Ahn
    • Journal of Bio-Environment Control
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    • v.32 no.1
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    • pp.48-56
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    • 2023
  • High-pressure sodium (HPS) lamps have been widely used as a useful supplemental light source to emit sufficient photosynthetically active radiation and provide a radiant heat, which contribute the heat requirement in greenhouses. The objective of this study to analyze the thermal characteristics of HPS lamp and thermal behavior in supplemented greenhouse, and evaluate the performance of a horizontal leaf temperature of sweet pepper plants using computational fluid dynamics (CFD) simulation. We simulated horizontal leaf temperature on upper canopy according to three growth stage scenarios, which represented 1.0, 1.6, and 2.2 plant height, respectively. We also measured vertical leaf and air temperature accompanied by heat generation of HPS lamps. There was large leaf to air temperature differential due to non-uniformity in temperature. In our numerical calculation, thermal energy of HPS lamps contributed of 50.1% the total heat requirement on Dec. 2022. The CFD model was validated by comparing measured and simulated data at the same operating condition. Mean absolute error and root mean square error were below 0.5, which means the CFD simulation values were highly accurate. Our result about vertical leaf and air temperature can be used in decision making for efficient thermal energy management and crop growth.