• Journal of the Institute of Convergence Signal Processing
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• v.9 no.1
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• pp.82-88
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• 2008

In this paper, Runge-Kutta (RK)-Butcher algorithm is proposed to study the periodic and oscillatory problems. Simulation results obtained using RK-Butcher algorithms and the classical fourth order Runge-Kutta (RK(4)) methods are compared with the exact solutions of a few periodic and oscillatory problems to confirm the performance of the proposed algorithm. The simulation results from RK-Butcher algorithms are always found to be very close to the exact solutions of these problems. Further, it is found that the RK-Butcher algorithm is superior when compared to RK(4) methods in terms of accuracy. The RK-Butcher algorithm can be easily implemented in a programming language and a more accurate solution may be obtained for any length of time. RK-Butcher algorithm is applicable as a good numerical algorithm for studying the problems of orbit and two body as it gives the nearly identical solutions.

• Interaction and multiscale mechanics
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• v.6 no.2
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• pp.173-195
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• 2013

Meshfree methods are known to have the capability to overcome the strict regularization requirements and numerical instabilities that encumber the finite element method (FEM) in large deformation problems. They are also more naturally suited for problems involving material perforation and fragmentation. To take advantage of the high efficiency of FEM and high accuracy of meshfree methods, a coupled finite element (FE) and reproducing kernel (RK, one of the meshfree approximations) formulation is described in this paper. The coupling of FE and RK approximation is implemented in an evolutionary fashion, where the extent and location of the evolution is dependent on a triggering criteria provided by the material constitutive laws. To enhance computational efficiency, Gauss quadrature is applied to integrate both FE and RK domains so that no state variable transfer is required when mesh conversion is performed. To control the hourglassing that might occur with 1-point integrated hexahedral grids, viscous type hourglass control is implemented. Meanwhile, the FEM version of the K&C concrete (KCC) model was modified to make it applicable in both FE and RK formulations. Results using this code and the KCC model are shown for the modeling of concrete responses under quasi-static, blast and impact loadings. These analyses demonstrate that fragmentation phenomena of the sort commonly observed under blast and impact loadings of concrete structures was able to be realistically captured by the coupled formulation.

• Journal of Ginseng Research
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• v.43 no.1
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• pp.10-19
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• 2019

Background: Frequent overdose of paracetamol (APAP) has become the major cause of acute liver injury. The present study was designed to evaluate the potential protective effects of ginsenoside Rk1 on APAP-induced hepatotoxicity and investigate the underlying mechanisms for the first time. Methods: Mice were treated with Rk1 (10 mg/kg or 20 mg/kg) by oral gavage once per d for 7 d. On the 7th d, allmice treated with 250mg/kg APAP exhibited severeliverinjury after 24 h, and hepatotoxicitywas assessed. Results: Our results showed that pretreatment with Rk1 significantly decreased the levels of serum alanine aminotransferase, aspartate aminotransferase, tumor necrosis factor, and interleukin-$1{\beta}$ compared with the APAP group. Meanwhile, hepatic antioxidants, including superoxide dismutase and glutathione, were elevated compared with the APAP group. In contrast, a significant decrease in levels of the lipid peroxidation product malondialdehyde was observed in the ginsenoside Rk1-treated group compared with the APAP group. These effects were associated with a significant increase of cytochrome P450 E1 and 4-hydroxynonenal levels in liver tissues. Moreover, ginsenoside Rk1 supplementation suppressed activation of apoptotic pathways by increasing Bcl-2 and decreasing Bax protein expression levels, which was shown using western blotting analysis. Histopathological observation also revealed that ginsenoside Rk1 pretreatment significantly reversed APAP-induced necrosis and inflammatory infiltration in liver tissues. Biological indicators of nitrative stress, such as 3-nitrotyrosine, were also inhibited after pretreatment with Rk1 compared with the APAP group. Conclusion: The results clearly suggest that the underlying molecular mechanisms in the hepatoprotection of ginsenoside Rk1 in APAP-induced hepatotoxicity may be due to its antioxidation, antiapoptosis, anti-inflammation, and antinitrative effects.

• Journal of Ginseng Research
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• v.43 no.4
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• pp.618-624
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• 2019

Background: Ginsenoside Re (Re) is one of the major components of Panax ginseng Meyer. Ginsenoside $Rk_3$ ($Rk_3$) is a secondary metabolite of Re. The aim of this study was to investigate and compare the effects and underlying mechanisms of Re and $Rk_3$ on cyclophosphamide-induced myelosuppression. Methods: The mice myelosuppression model was established by intraperitoneal (i.p.) injection of cyclophosphamide. Peripheral blood cells, bone marrow nucleated cells, and colony yield of hematopoietic progenitor cells in vitro were counted. The levels of erythropoietin, thrombopoietin, and granulocyte macrophage colony-stimulating factor in plasma were measured by enzyme-linked immunosorbent assay. Bone marrow cell cycle was performed by flow cytometry. The expression of apoptotic protein bcl-2, bax, and caspase-3 was detected by Western blotting. Results: Both Re and $Rk_3$ could improve peripheral blood cells, bone marrow nucleated cell counts, thymus index, and spleen index. Furthermore, they could enhance the yield of colonies cultured in vitro and make the levels of granulocyte macrophage colony-stimulating factor, erythropoietin, and thrombopoietin normal, reduce the ratio of $G_0/G_1$ phase cells, and increase the proliferation index. Finally, Re and $Rk_3$ could upregulate the expression of bcl-2, whereas they could downregulate the expression of bax and caspase-3. Conclusion: Re and $Rk_3$ could improve the hematopoietic function of myelosuppressed mice. The effect of $Rk_3$ was superior to that of Re at any dose. Regulating the levels of cytokines, promoting cells enter the normal cell cycle, regulating the balance of bcl-2/bax, and inhibiting the expression of caspase-3 may be the effects of Re and $Rk_3$ on myelosuppression.

• Journal of Ginseng Research
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• v.47 no.5
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• pp.645-653
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• 2023

Background: Changes to work-life balance has increased the incidence of cervical cancer among younger people. A minor ginseng saponin known as ginsenoside Rk1 can inhibit the growth and survival of human cancer cells; however, whether ginsenoside Rk1 inhibits HeLa cell proliferation is unknown. Methods and results: Ginsenoside Rk1 blocked HeLa cells in the G0/G1 phase in a dose-dependent manner and inhibited cell division and proliferation. Ginsenoside Rk1 markedly also activated the apoptotic signaling pathway via caspase 3, PARP, and caspase 6. In addition, ginsenoside Rk1 increased LC3B protein expression, indicating the promotion of the autophagy signaling pathway. Protein processing in the endoplasmic reticulum signaling pathway was downregulated in Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses, consistent with teal-time quantitative PCR and western blotting that showed YOD1, HSPA4L, DNAJC3, and HSP90AA1 expression levels were dramatically decreased in HeLa cells treated with ginsenoside Rk1, with YOD1 was the most significantly inhibited by ginsenoside Rk1 treatment. Conclusion: These findings indicate that the toxicity of ginsenoside Rk1 in HeLa cells can be explained by the inhibition of protein synthesis in the endoplasmic reticulum and enhanced apoptosis, with YOD1 acting as a potential target for cervical cancer treatment.

• Journal of Ginseng Research
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• v.44 no.3
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• pp.490-495
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• 2020

Background: Ginsenoside Rk1, a saponin component isolated from heat-processed Panax ginseng Meyer, has been implicated in the regulation of antitumor and anti-inflammatory activities. Although our previous studies have demonstrated that ginsenoside Rg3 significantly attenuated the activation of NMDA receptors (NMDARs) in hippocampal neurons, the effects of ginsenosides Rg5 and Rk1, which are derived from heat-mediated dehydration of ginsenoside Rg3, on neuronal NMDARs have not yet been elucidated. Methods: We examined the regulation of NMDARs by ginsenosides Rg5 and Rk1 in cultured rat hippocampal neurons using fura-2-based calcium imaging and whole-cell patch-clamp recordings. Results: The results from our investigation showed that ginsenosides Rg3 and Rg5 inhibited NMDARs with similar potencies. However, ginsenoside Rk1 inhibited NMDARs most effectively among the five compounds (Rg3, Rg5, Rk1, Rg5/Rk1 mixture, and protopanaxadiol) tested in cultured hippocampal neurons. Its inhibition is independent of the NMDA- and glycine-binding sites, and its action seems to involve in an interaction with the polyamine-binding site of the NMDAR channel complex. Conclusion: Taken together, our results suggest that ginsenoside Rk1 might be a novel component contributable to the development of ginseng-based therapeutic treatments for neurodegenerative diseases.

• Journal of Ginseng Research
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• v.41 no.1
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• pp.78-84
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• 2017

Background: In the present study, metabolite profiles of ginsenosides Rk1 and Rg5 from red ginseng or red notoginseng in zebrafish were qualitatively analyzed with ultraperformance liquid chromatography/quadrupole-time-of-flight MS, and the possible metabolic were pathways proposed. Methods: After exposing to zebrafish for 24 h, we determined the metabolites of ginsenosides Rk1 and Rg5. The chromatography was accomplished on UPLC BEH C18 column using a binary gradient elution of 0.1% formic acetonitrile-0.1% formic acid water. The quasimolecular ions of compounds were analyzed in the negative mode. With reference to quasimolecular ions and MS2 spectra, by comparing with reference standards and matching the empirical molecular formula with that of known published compounds, and then the potential structures of metabolites of ginsenosides Rk1 and Rg5 were acquired. Results: Four and seven metabolites of ginsenoside Rk1 and ginsenoside Rg5, respectively, were identified in zebrafish. The mechanisms involved were further deduced to be desugarization, glucuronidation, sulfation, and dehydroxymethylation pathways. Dehydroxylation and loss of C-17 residue were also metabolic pathways of ginsenoside Rg5 in zebrafish. Conclusion: Loss of glucose at position C-3 and glucuronidation at position C-12 in zebrafish were regarded as the primary physiological processes of ginsenosides Rk1 and Rg5.

• Journal of Industrial Technology
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• v.44 no.1
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• pp.17-23
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• 2024

K-means is a popular and efficient data clustering method which is one of the most important technique in data mining. K-means is sensitive for initialization and has the possibility to be stuck in local optimum because of hill climbing clustering method. Therefore, we need a robust K-means (RK-means) not only to reduce this possibility but also to increase the probability to search the global optimal clustering solution. The objective of this paper is to propose RK-means with best initial solution from good solutions with good central data for each cluster. The central data of each cluster is selected based on Roulette wheel probabilistic selection using sum of relative distance rate of each data. They have a problem in high density data because they deterministically select the central data for just one initial solution of K-medoid. Our proposed initial solution is the good starting point to find the robust solution by K-means with reducing the possibility being stuck in local optimal solutions. The performance of proposed RK-means data clustering is validated using machine learning repository datasets (Iris, Wine, Glass, Vowel, Cloud) comparing to original K-means by experiment and analysis. Our simulation shows that RK-means using probabilistically relative distance rate are better than K-means with random initialization. The minimum squared distance by RK-means with smaller deviation is lower than that by K-means with higher deviation. RK-means is competitive comparing to data clustering methods based on simulated annealing (SA) and hybrid K-means with SA (KSA & KSAK).

• The Journal of Asian Finance, Economics and Business
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• v.2 no.3
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• pp.23-32
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• 2015

The aim of this work is to study the specifics of demographic processes in the Republic of Kazakhstan and to justify proposals concerning improvement of tendencies and methods of realizations of demographic policy in the country. The following methods have been used in this study: the principles of system approach; methods of statistical and comparative economic analysis, sociological analysis; method of expert evaluations; generalization and system analysis. Statistical method is based on the accountability of statistics office of the Ministry of National Economy of the Republic of Kazakhstan (RK) and on the results of sociological studies. The official statistic information, materials of the population census of the RK, materials of the Ministry of Health and Social Development of RK, data from author's studies, carried out in the Institute of Economy of science committee of Ministry of Education and Science of the Republic of Kazakhstan were used in this study. The conclusion about positive tendencies in demographic processes and about necessity of improvement of demographic policy was made. A number of proposals and recommendations concerning creation of favorable social and economic conditions for improvement of demographic situation, enhancement of institutional basis of demographic policy, forms and methods of its realization considering priorities of future development of Kazakhstan were justified.

• The Journal of the Korea Contents Association
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• v.14 no.10
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• pp.454-462
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• 2014

With the development of location aware technologies and mobile devices, location-based services have been studied. To provide location-based services, many researchers proposed methods for processing various query types with Mapreduce(MR). One of the proposed methods, is a Reverse k-nearest neighbor(RkNN) query processing method with MR. However, the existing methods spend too much cost to process the continuous RkNN query. In this paper, we propose an efficient continuous RkNN query processing method with MR to resolve the problems of the existing methods. The proposed method uses the 60-degree-pruning method. The proposed method does not need to reprocess the query for continuous query processing because the proposed method draws and monitors the monitoring area including the candidate objects of a RkNN query. In order to show the superiority of the proposed method, we compare it with the query processing performance of the existing method.