• Proceedings of the Korean Society of Crop Science Conference
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• 2003.10a
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• pp.50-51
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• 2003

• Applied Biological Chemistry
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• v.48 no.4
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• pp.339-344
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• 2005

Rice blast, which is caused by the fungus Magnaporthe grisea, is one of the most destructive diseases of rice. To identify genes involving in the signal transduction pathways that mediate rice blast resistance, we screened over 2,000 mutant lines of a highly resistant variety RIL260 that were generated by using a DEB (1, 3-Butadiene diepoxide) treatment method. In the mutant population, the frequency of albino plants was 6.7%, indicating that this population has a high frequency of mutations in the genome. The primary screening identified 29 mutant plants that exhibit a complete or partial loss of the resistance to rice blast. Among them, M5465, the most susceptible line, was subsequently examined by DNA gel-blot experiments using DNA molecular markers of Pi5(t) that has been previously identified as a durable resistance locus in RIL260. The result revealed that a large deletion and rearrangement of genomic DNA occurred in the Pi5(t) locus. The results suggest that DEB can be used as an efficient mutagen to induce large scale mutations in the rice genome. The isolated mutants should be useful for elucidating the Pi5(t)-mediated signaling pathways of rice blast resistance.

• Journal of Crop Science and Biotechnology
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• v.11 no.3
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• pp.171-176
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• 2008

The agronomic traits, such as days to flowering and maturity, plant height, 100-seed weight and seed filling period, are quantitatively inherited and important characters in soybean(Glycine max L. Merr.). A total of 126 $F_5$ recombinant inbred lines(RILs) developed from the cross of PI 171451$\times$Hwaeomputkong were used to identify quantitative trait loci(QTLs) for days to flowering(FD), days to maturity(MD), plant height(PH), 100-seed weight(SW), number of branches(NB) and seed filling period(FP). A total of 136 simple sequence repeat(SSR) markers segregated in a RIL population were distributed over 20 linkage groups(LGs), covering 1073.9 cM of the soybean genome with the average distance between adjacent markers of 7.9 cM. Five independent QTLs were identified for FD, three for MD, two for PH, three for SW, one for NB and one for FP. Of these, three QTLs were related to more than two traits of FD, MD, PH, NB and FP and mapped near the same positions on LGs H and O. Thus, these traits could be correlated with biologically controlled major QTLs in this soybean RIL population.

• Proceedings of the Korean Society of Crop Science Conference
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• 2000.04a
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• pp.34-35
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• 2000

• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.4
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• pp.414-421
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• 2019

Pod shattering during the maturing stage causes a serious yield loss in soybean. It is the main limiting factor of soybean cultivation and mechanization. It is important to develop varieties suitable for mechanical harvesting and to develop energy-efficient agricultural machinery to save labor and costs. 'Daewonkong,' developed by the National Institute of Crop Science (NICS) in 1997, is an elite cultivar that occupies more than 80% of the soybean cultivation area in Korea because of its strong tolerance to pod shattering. The objectives of this study were to investigate the variation in pod shattering degree in a RIL population developed from a 'Daewonkong' parent and to select promising lines with pod shattering tolerance. 'Daewonkong' demonstrated a high level of tolerance to pod shattering compared to the 'Tawonkong' and 'Saeolkong' varieties, with no shattered pods after 72 hours of drying. Screening of pod shattering showed a clear distinction between the tolerant and susceptible varieties. Also, the distribution of shattering pod ratio in the two populations showed a similar pattern for three years. The promising lines with pod shattering tolerance included 27 lines in the 'Daewonkong'×'Tawonkong' population and 21 lines in the 'Daewonkong'×'Saeolkong' population. The promising lines are expected to be widely used as breeding parents for creating soybean cultivars with pod shattering tolerance.

• Genomics & Informatics
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• v.19 no.2
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• pp.19.1-19.9
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• 2021

Plant height is an important component of plant architecture and significantly affects crop breeding practices and yield. We studied DNA variations derived from F5 recombinant inbred lines (RILs) with 96.8% homozygous genotypes. Here, we report DNA variations between the normal and dwarf members of four lines harvested from a single seed parent in an F6 RIL population derived from a cross between Glycine max var. Peking and Glycine soja IT182936. Whole genome sequencing was carried out, and the DNA variations in the whole genome were compared between the normal and dwarf samples. We found a large number of DNA variations in both the dwarf and semi-dwarf lines, with one single nucleotide polymorphism (SNP) per at least 3.68 kb in the dwarf lines and 1 SNP per 11.13 kb of the whole genome. This value is 2.18 times higher than the expected DNA variation in the F6 population. A total of 186 SNPs and 241 SNPs were discovered in the coding regions of the dwarf lines 1282 and 1303, respectively, and we discovered 33 homogeneous nonsynonymous SNPs that occurred at the same loci in each set of dwarf and normal soybean. Of them, five SNPs were in the same positions between lines 1282 and 1303. Our results provide important information for improving our understanding of the genetics of soybean plant height and crop breeding. These polymorphisms could be useful genetic resources for plant breeders, geneticists, and biologists for future molecular biology and breeding projects.

• Proceedings of the Korean Society of Crop Science Conference
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• 2001.09a
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• pp.142-143
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• 2001

• Proceedings of the Korean Society of Crop Science Conference
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• 2018.04a
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• pp.28-28
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• 2018

• Proceedings of the Korean Society of Crop Science Conference
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• 2018.10a
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• pp.217-217
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• 2018

• KOREAN JOURNAL OF CROP SCIENCE
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• v.65 no.3
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• pp.172-181
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• 2020

Koshihikari has been one of the most popular rice cultivars with good eating quality since the 1960s despite its susceptibility to blast disease and lodging. To map the genes controlling blast resistance and to develop promising blast-resistant breeding lines inheriting Koshihikari's high eating quality, a recombinant inbred line (RIL) population was developed from a cross between Koshihikari and a blast resistance donor with early maturity, Baegilmi. A total of 394 Koshihikari × Baegilmi RILs (KBRIL), and the two parents, were evaluated for blast resistance and major agronomic traits including heading date, culm length, panicle length, and tiller number. A linkage map encompassing 1,272.7 cM was constructed from a subset of the KBRIL (n = 142) using 130 single nucleotide polymorphisms. Two quantitative trait loci (QTL) for blast resistance, qBL1.1 harboring Pish/Pi35 and qBL2.1 harboring Pib, were mapped onto chromosomes 1 and 2, respectively. qBL1.1 was detected in both of the experimental sites, Namwon and Jeonju, while qBL2.1 was only detected in Namwon. qBL1.1 and qBL2.1 did not affect agronomic traits, including heading date, culm length, panicle length, and tiller number. From the 394 KBRILs, lines that were phenotypically similar to Koshihikari were selected according to heading date and culm length and were further divided into the following two groups based on blast resistance: Koshishikari-type blast resistant lines (KR, n = 15) and Koshishikari-type blast susceptible lines (KS, n = 15). Although no significant differences were observed in the major agronomic traits between the two groups, the KR group produced a greater mean head rice ratio than the KS group. The present study provides useful materials for developing blast-resistant cultivars that inherit both Koshihikari's high eating quality and Baegilmi's blast resistance.