• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.3
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• pp.292-296
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• 2006

The antimicrobial activity of methanol extracts from 17 seaweeds was screened using a paper disc method and using three human skin pathogens: Staphylococcus aureus, S. epidermidis and Candia albicans. The serial extraction of Neorhodomela aculeata was also conducted using four different solvents (n-hexane, chloroform, ethyl acetate, and methanol) and the minimal inhibitory concentration (MIC) of each extract was examined for the three pathogens. Of the 17 seaweeds, the MeOH extracts of Ulva conglobata, N. aculeata and Symphyocladia latiuscula showed antimicrobial activities. For the extracts from N. aculeata and S. latiuscula, the inhibition zones were more than 10 mm in diameter against S. aureus and S. epidermidis, and >7mm for C. albicans. The inhibition zone of U. conglobata treatment was about 8 mm for S. aureus only. The MIC of each N. aculeata extract ranged from 8 to 32 mg/mL against the three human skin pathogens, and the lowest value (8 mg/mL was with the methanol extract. These results suggest that the MeOH extract of N. aculeata might be useful for developing new antibiotics against human skin pathogens.

• Journal of Plant Biotechnology
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• v.44 no.3
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• pp.287-295
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• 2017

Water temperature is one of the major factors that impacts the growth and life cycle of Pyropia tenera, one of the most valuable and cultivated marine red algae belonging to Bangiales (Rhodophytes). We analyzed transcriptome from gametophyte of P. tenera under normal and high temperature conditions, and identified four small heat shock proteins (sHSPs). They have no significant amino acid sequence homology with known proteins in public databases except PhsHSP22 from Pyropia haitanensis. PtsHSP19.3 gene responded to high temperature but slightly or not to desiccation, freezing or high salt condition. When the PtsHSP19.3 gene was overexpressed in Chlamydomonas reinhardtii, transformed Chlamydomonas lines revealed much higher growth rate than that of control cells under heat stress condition. Transformed cells also grew well in those of the control cell onto the medium containing high salt or $H_2O_2$. When the PtsHSP19.3 was fused to GFP and introduced into tobacco protoplast, fluorescence was detected at several spots. Results indicate that PtsHSP19.3 may form super-molecular assembles and be involved in tolerance to heat stress.

• Natural Product Sciences
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• v.18 no.2
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• pp.130-136
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• 2012

In the present work, we have collected 19 species of macroalgae (9 Phaeophta and 10 Rhodophyta) f rom all around of Korea: Dictyopteris divaricata, D. prolifera, Myelophycus cavus, Papenfussiella kuromo, Petalonia zosterifolia, Petrospongium rugosum, Rugulopteryx okamurae, Sargassum fulvellum, S. muticum, Callophyllis japonica, Gloiopeltis tenax, Gracilaria longissima, Gracilaria vermiculophylla, Grateloupia asiatica, Grateloupia lanceolata, Grateloupia sparsa, Grateloupia turuturu, Grateloupia sp, and Polyopes affinis. The macroalgal species were extracted by 70% ethanol (EtOH) for 24 h and evaluated its inhibitory effects on ${\alpha}$-glucosidase. Among ethanol extracts, Myelophycus cavus showed the most effectively inhibitory activity ($IC_{50}$, 2.17 ${\mu}g/ml$) against ${\alpha}$-glucosidase, followed by Sargassum fulvellum (<$IC_{50}$, 8.13 ${\mu}g/ml$), Dictyopteris prolifera ($IC_{50}$, 16.66 ${\mu}g/ml$), Rugulopteryx okamurae ($IC_{50}$, 50.63 ${\mu}g/ml$), and Petrospongium rugosum ($IC_{50}$, 101.62 ${\mu}g/ml$). Furthermore, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay showed no cytotoxicity on mouse pre-adipocytes cell line (3T3-L1). These results suggest that some edible macroalgae merit further evaluation for clinical usefulness as anti-diabetic functional foods.

• Fisheries and Aquatic Sciences
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• v.12 no.1
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• pp.70-77
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• 2009

Porphyra yezoensis is known to act as a biofilter against nutrient-rich effluent in seaweed-based integrated aquaculture systems. However, few studies have examined its physiological status under such conditions. In this study, we estimated the photosynthetic activity of P. yezoensis by chlorophyll fluorescence of PSII (${\Delta}F/F'm$ and relative $ETR_{max}$) using the Diving-PAM fluorometer (Walz, Germany). In addition, bioremediation capacity, tissue nutrients, and C:N ratio of P. yezoensis were investigated. The ammonium concentration in seawater of seaweed tank 4 decreased from $72.1{\pm}2.2$ to $33.8{\pm}0.4{\mu}M$ after 24 hours. This indicates the potential role of P. yezoensis in removing around 43% of ammonium from the effluents. Tissue carbon contents in P. yezoensis were constant during the experimental period, while nitrogen contents had increased slightly by 24 hours. In comparison with the initial values, the ${\Delta}F/F'm$ and $rETR_{max}$ of P. yezoensis had increased by about 20 and 40%, respectively, after 24 hours. This indicates that P. yezoensis condition improved or remained constant. These results suggest that chlorophyll fluorescence is a powerful tool in evaluating the physiological status of seaweeds in a seaweed-based integrated aquaculture system.

• ALGAE
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• v.23 no.4
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• pp.311-316
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• 2008

The seasonal variation of marine algal biomass and community were studied with nondestructive sampling and quadrat method during May 2006 to April 2008 in the intertidal and subtidal zone at Yokjido. As result, total 120 species, 13 Chlorophyta, 34 Phaeophyta, 71 Rhodophyta and 2 Spermatophyta were identified in this study. The major species which contributed significantly to the total biomass were Ulva pertusa, Undaria pinnatifida, Ecklonia stolonifera, Sargassum horneri, S. serratifolium, Gelidium amansii and Prionitis cornea. The mean biomass was 235.5 g wet wt m$^{-2}$ in intertidal, 1,038.9 g wet wt m$^{-2}$ in 1 m, 1,013.1 g wet wt m$^{-2}$ in 5 m, and 19.7 g wet wt m$^{-2}$ in 10 m depths of mean sea level. Seasonal biomass change in intertidal zone, the peak season was in spring, while the lowest was in autumn. The vertical distribution of marine vegetation was characterized by Ulva pertusa - Gelidium divaricatum - Gloiopeltis furcata - Sargassum thunbergii - Hizikia fusiformis at intertidal zone, and Caulerpa okamurae - Gelidium amansii - Chondrus ocellatus - Ecklonia stolonifera - Sargassum horneri - Gracilaria textorii at subtidal zone. These result indicates that the marine algal species and biomass of 1 m and 5 m depths in subtidal zone are greater than intertidal zone, and Ecklonia stolonifera and Sargassum spp. are the dominant species of Yokjido.

• Journal of Plant Biology
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• v.38 no.4
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• pp.389-398
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• 1995

The species composition and biomass of intertidal benthic algae at 12 widely dispersed stations representative of mid-western coast of Korea were studied. A total of 151 species of marine algae including 19 Cyanophyta, 20 Chlorophyta, 32 Phaeophyta and 80 Rhodophyta is listed. Three species were found at all 12 stations: Sargassum thunbergii, Stylonema alsidii and Gracilaria verrucosa. Biomass per unit area exhibited a wide range of variation, ranging from a low of 41 g dry $wt{\cdot}m^{-2}$ at both Paeksajang and Taechon to a high of 549 g dry $wt{\cdot}m^{-2}$ at Uihangri, the northernmost station of the study area. The latter value is higher than those reported from any other stations in western coast of Korea. Biomass was composed largely of Phaeophyta at nearly every station. Sargassum thunbergii was dominant over the study area. The distribution of Corallina spp., however, is disjunct, since those occur as dominant species in biomass at the northern stations and to a lesser extent at the southern stations, but are nearly absent from the middle part of the study area.

• Journal of Navigation and Port Research
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• v.30 no.4
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• pp.309-314
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• 2006

Community structure and biodiversity of benthic marine algae were studied in the rocky shore of Phohang New Port, Yeoungil Bay. A total of 79 species of marine algae including 8 Chlorophyta, 23 Phaeophyta and 48 Rhodophyta are listed. The dominant algal species were Corallina pilulifera in all seasons, and Sargassum homeri in winter. Lomentaria catenata, Lomentaria hakodatensis, Grateloupia laceolata, Sargassum thunbergii, Chondria crassicaulis, Ulva pertusa, and Hypnea charoides were subdominant at different seasons. The algal biomass per unit area of the benthic algal community in Pohang New Port under construction was 14506 - 398.93 g dry wt m-2, which means that its annually averaged value is 273.45 g dry wt m-2 . The seasonal change in algal biomass showed highs in winter and spring, and lows in summer and autumn. The algal species diversity (H') based on algal biomass was 2.07 annually in average, with 2.42 in winter, 2.65 in spring, 1.79 in summer and 1.43 in autumn. It was high in winter and spring seasons and low in summer and autumn seasons. It is caused by that spring and winter are the profitable growth time for algae and species components in this study were dominated by the annual or seasonal rather than perennial plants.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2003.11a
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• pp.38-52
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• 2003

Seaweeds are large algae that grow in a saltwater or marine environment. There are over 400 species of seaweeds in the bay of Korea. Most seaweeds are divided into three groups according to their color : the green seaweeds(division Chlorophyta), the brown(Phanphyta) and the red(Rhodophyta), Seaweeds have been harvested for use as food, fertilizer, animal feed and medicines for thousands years. In the past decade, there has been increasing interest in using seaweeds for animal feedstuffs and feed supplements as their health qualities are becoming better known. In this review some perspectives on nutritional values and the role of functionality ingredients of various seaweeds will be also discussed. Ecklonia cava kjellman(EC), a perennial seaweed, is one of the main components of the marine forests along the East and South coasts of Korea. We conducted three experiments to evaluate the nutritional values of EC and the crude lectin extracted residues from EC(LEEC) and to investigate their dietary effects on productivity and immunocompetence in poultry. The crude protein content of EC was relatively low(about 10 ％) and its amino acids were poorly available. But, the results of our study suggested that EC can be used into broiler feed up to 3 ％ without any adverse effect, if its nutritional values are well evaluated prior to use. Similar results of dietary LECC up to 1.0 ％ level on egg production and egg quality have been obtained in layers. The concentrations of serum TNF-${\alpha}$ after Salmonella challenge were rapidly increased in accordance with the level of LEEC. These results indicated that LEEC can be used as a feed additives for enhancing of immunocompetence in poultry.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.8 no.4
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• pp.420-425
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• 2003

Species composition, community structure and biodiversity of marine benthic community were studied in the intertidal zone of Seoam, Busan. A total of 75 species of benthic marine plants including 4 Cyanophyta, 6 Chlorophyta, 17 Phaeophyta, 47 Rhodophyta and 1 Magnoliophyta are listed. The dominant marine plants were melobesioidean algae, Chondracanthus tenellus, Sargasium thunbergii, Corallina spp., and Phyllospadix japonica and Ulva pertusa was added in summer. Chthamalus challengeri and Mytilus edulis were dominant zoobenthic species in the upper and middle intertidal zone. The algal species diversity index based on coverage was 1.81; 2.25 from frequency; 2.19 from average of total frequency and coverage, and 1.80 from importance value. The algal diversity indices estimated from different sources were quite different. This means that the index value changes depending on the sources used to calculate the species diversity index, indicating how important it is to select the based data and that it is necessary to standardize the methodology when studying later the algal diversity index. On the other hand, the number of species identified in this study has been found to be reduced by 65% at highest, compared with the result of the investigation that was conducted in the coast of Busan.

• ALGAE
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• v.36 no.3
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• pp.165-174
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• 2021

Radicilingua Papenfuss and Calonitophyllum Aregood are two small genera of the family Delesseriaceae that consist of only three and one taxonomically accepted species, respectively. The type species of these genera, Radicilingua thysanorhizans from England and Calonitophyllum medium from the Americas, are morphologically very similar, with the only recognized differences being vein size and procarp development. To date, only other two species were recognized inside the genus Radicilingua: R. adriatica and R. reptans. In this study, we analysed specimens of Radicilingua collected in the Adriatic and Ionian Sea (Mediterranean), including a syntype locality of R. adriatica (Trieste, northern Adriatic Sea), alongside material from near the type locality of R. thysanorhizans (Torpoint, Cornwall, UK). The sequences of the rbcL-5P gene fragment here produced represent the first molecular data available for the genus Radicilingua. Phylogenetic reconstruction showed that the specimens from the Adriatic and Ionian Seas were genetically distinct from the Atlantic R. thysanorhizans, even if morphologically overlapping with this species. A detailed morphological description of the Mediterranean specimens, together with an accurate literature search, suggested that they were distinct also from R. adriatica and R. reptans. For these reasons, a new species was here described to encompass the Mediterranean specimens investigated in this study: R. mediterranea Wolf, Sciuto & Sfriso. Moreover, in the rbcL-5P tree, sequences of the genera Radicilingua and Calonitophyllum grouped in a well-supported clade, distinct from the other genera of the subfamily Nitophylloideae, leading us to propose that Calonitophyllum medium should be transferred to Radicilingua.