Browse > Article
http://dx.doi.org/10.5010/JPB.2017.44.3.287

Overexpression of the Small Heat Shock Protein, PtsHSP19.3 from Marine Red Algae, Pyropia tenera (Bangiales, Rhodophyta) Enhances Abiotic Stress Tolerance in Chlamydomonas  

Jin, Yujin (Department of Biology Education and Kumho Life Science Laboratory, Chonnam National University)
Yang, Sungwhan (Department of Biology Education and Kumho Life Science Laboratory, Chonnam National University)
Im, Sungoh (Department of Biology Education and Kumho Life Science Laboratory, Chonnam National University)
Jeong, Won-Joong (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology)
Park, EunJeong (Seaweed Research Center, National Fisheries Research and Development Institute)
Choi, Dong-Woog (Department of Biology Education and Kumho Life Science Laboratory, Chonnam National University)
Publication Information
Journal of Plant Biotechnology / v.44, no.3, 2017 , pp. 287-295 More about this Journal
Abstract
Water temperature is one of the major factors that impacts the growth and life cycle of Pyropia tenera, one of the most valuable and cultivated marine red algae belonging to Bangiales (Rhodophytes). We analyzed transcriptome from gametophyte of P. tenera under normal and high temperature conditions, and identified four small heat shock proteins (sHSPs). They have no significant amino acid sequence homology with known proteins in public databases except PhsHSP22 from Pyropia haitanensis. PtsHSP19.3 gene responded to high temperature but slightly or not to desiccation, freezing or high salt condition. When the PtsHSP19.3 gene was overexpressed in Chlamydomonas reinhardtii, transformed Chlamydomonas lines revealed much higher growth rate than that of control cells under heat stress condition. Transformed cells also grew well in those of the control cell onto the medium containing high salt or $H_2O_2$. When the PtsHSP19.3 was fused to GFP and introduced into tobacco protoplast, fluorescence was detected at several spots. Results indicate that PtsHSP19.3 may form super-molecular assembles and be involved in tolerance to heat stress.
Keywords
Red algae; Pyropia tenera; small heat shock protein; Heat tolerance; Abiotic stress tolerance;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Wehmeyer N, Vierling E (2000) The expression of small heat shock proteins in seeds responds to discrete developmental signals and suggests a general protective role in desiccation tolerance. Plant Physiol 122:1099-1108   DOI
2 Zhai M, Sun Y, Jia C, Peng S, Liu Z, Yang G (2016) Over-expression of JrsHSP17.3 gene from Juglans regia confer the tolerance to abnormal temperature and NaCl stresses. J Plant Biol 59:549-558   DOI
3 Zhang K, Ezemaduka AN, Wang Z, Hu H, Shi X, Liu C, Lu X, Fu X, Chang Z, Yin CC (2015) A Novel Mechanism for Small Heat Shock Proteins to Function as Molecular Chaperones. Scientific reports. 5:8811   DOI
4 Yan H, Zhang A, Chen J, He X, Xu B, Xie G, Miao Z, Zhang X, Huang L (2017) Geneome-wide analysis of the PvHsp20 family in switchgrass: motif, genomic organization, and identification of stress or developmental-related Hsp20s. Front Plant Sci 8:1024   DOI
5 Basha E, O’Neill H, Vierling E (2012) Small heat shock proteins and alpha-crystallins: dynamic proteins with flexible functions. Trends Biochem Sci 37:106-117   DOI
6 Blouin NA, Brodie JA, Grossman AC, Xu P, Brawley SH (2011) Porphyra: a marine crop shaped by stress. Trends Plant Sci 16:29-37   DOI
7 Choi S, Hwang MS, Im SO, Kim NJ, Jeong WJ, Park EJ, Gong YG, Choi DW (2013) Transcriptome sequencing and comparative analysis of the gametophyte of Pyropia tenera under normal and high-temperature condition. J Appl Phycol 25:1237-1246   DOI
8 Hwang MS, Chung IK, Oh YS (1997) Temperature responses of Porphyra tenera Kjellman and P. yezoensis Ueda (Bangiales, Rhodophyta) from Korea. Algae 12:207-213
9 Hwang MS, Kim SM, Ha DS, Baek JM, Kim HS, Choi HG (2005) DNA sequences and identification of Porphyra cultivated by natural seeding on the southwest coast of Korea. Algae 20:183-196   DOI
10 Im S, Choi S, Hwang MS, Park EJ, Jeong WJ, Choi DW (2015) De novo assembly of transcriptome from the gametophyte of the marine red algae Pyropia seriata and identification of abiotic stress response genes. J Appl Phycol 27:1343-1353   DOI
11 McLachlan J (1973) Growth media-marine. In: Stein JR (ed) Handbook of phycological methods, Cambridge University Press, New York
12 Kim KK, Kim R, Kim S-H (1998) Crystal structure of a small heat shock protein. Nature 394:595-599   DOI
13 Kotak S, Vierling E, Baumlein H, von Koskull-Doring P (2007) A novel transcriptional cascade regulating expression of heat stress proteins during seed development of Arabidopsis. Plant Cell 19:182-195   DOI
14 Lambert W, Koeck PJ, Ahrman E, Purhonen P, Cheng K, Elmlund D, Hebert H, Emanuelsson C (2011) Subunit arrangement in the dodecameric chloroplast small heat shock protein Hsp21. Protein Science 20:291-301   DOI
15 Lee BH, Won SH, Lee HS, Miyao M, Chung WI, Kim IJ, Jo JK (2000) Expression of the chloroplast-localized small heat shock protein by oxidative stress in rice. Gene 245:283-290   DOI
16 Malik MK, Slovin JP, Hwang CH, Zimmerman JL (1999) Modified expression of a carrot small heat shock protein gene, hsp17.7, results in increased or decreased thermotolerance double danger. Plant J 20:89-99   DOI
17 Mu C, Zhang S, Yu G, Chen N, Li X, Liu H (2013) overexpression of small heat shock protein LimHSP16.45 in Arabidopsis enhances tolerance to abiotic stresses. Plos One 8:12
18 Neta-Sharir I, Isaacson T, Lurie S, Weiss D (2005) Dual role for tomato heat shock protein 21: protecting photosystem II from oxidative stress and promoting color changes during fruit maturation. Plant Cell 17:1829-1838   DOI
19 Ruibal C, Castro A, Carballo V, Szabados L, Vidal S (2013) Recovery from heat, salt and osmotic stress in Physcomitrella patens requires a functional small heat shock protein PpHsp16.4. BMC Plant Biol 13:174   DOI
20 Sahoo D, Tang X, Yarish C (2002) Porphyra-the economic seaweed as a new experimental system. Curr Sci India 83:1313-1316
21 Scharf KD, Siddique M, Vierling E (2001) The expanding family of Arabidopsis thaliana small heat stress proteins and a new family of proteins containing alpha-crystallin domains Acd proteins. Cell Stress & Chaperones 6:225-237   DOI
22 Schroda M, Vallon O (2009) Chaperones and proteases. In: Stern DB (ed) Chlamydomonas source book 2nd edn, volume 2, Elsevier, San Diego
23 Siddique M, Gernhard S, von Koskull-Doring P, Vierling E, Scharf KD (2008) The plant sHSP superfamily: five new members in Arabidopsis thaliana with unexpected properties. Cell Stress and Chaperones 13:183-17   DOI
24 Van Montfort R, Slingsby C, Vierling E. (2002) Structure and function of the small heat shock protein/alpha-crystallin family of molecular chaperones. Advances in Protein Chem 59:105-156
25 Stengel F, Baldwin AJ, Painter AJ, Jaya N, Basha E, Kay LE, Vierling E, Robinson CV, Benesch JLP (2010) Quaternary dynamics and plasticity underlie small heat shock protein chaperone function. Proc Nat Acad Sci USA 107:2007-2012   DOI
26 Sun W, Van Montagu, Verbruggen N (2002) small heat shock proteins and stress tolerance in plants. Biochemica et Biophysica Acta 1577:1-9   DOI
27 Sun W, Bernard C, van de Cotte B, van Montagu M, Verbruggen N (2001) At-HSP17.6A, encoding a small heat-shock protein in Arabidopsis, can enhance osmotolerance upon overexpression. Plant J 27:407-415   DOI
28 Wang W, Vinocur B, Shoseyov O, Altman A (2004) Role of plant heat-shock proteins and molecular chaperons in the abiotic stress response. Trends Plant Sci 9:244-252   DOI
29 Van Montfort RL, Basha E, Friedrich KL, Slingsby C, Vierling E (2001) Crystal structure and assembly of a eukaryotic small heat shock protein. Nature Structural Biology 8:1025-1030   DOI
30 Volkov RA, Panchuk II, Schoffl F (2005) Small heat shock proteins are differentially regulated during pollen development and following heat stress in tobacco. Plant Mol Biol 57:487-50   DOI
31 Waters ER (2013) The evolution, function, structure, and expression of the plant sHSPs. J Exp Bot 64:31.403   DOI
32 Waters ER, Aevermann BD, Sanders-Reed Z (2008) Comparative analysis of the small heat shock proteins in three angiosperm genomes identifies new subfamilies and reveals diverse evolutionary patterns. Cell Stress & Chaperones 13:127-142   DOI