• Journal of the Korean Data and Information Science Society
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• v.15 no.4
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• pp.743-752
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• 2004

In this paper, we develop the Bayesian model selection procedure using the reference prior for comparing two nested model such as the independent and intraclass models using the distance or divergence between the two as the basis of comparison. A suitable criterion for this is the power divergence measure as introduced by Cressie and Read(1984). Such a measure includes the Kullback -Liebler divergence measures and the Hellinger divergence measure as special cases. For this problem, the power divergence measure turns out to be a function solely of $\rho$, the intraclass correlation coefficient. Also, this function is convex, and the minimum is attained at $\rho=0$. We use reference prior for $\rho$. Due to the duality between hypothesis tests and set estimation, the hypothesis testing problem can also be solved by solving a corresponding set estimation problem. The present paper develops Bayesian method based on the Kullback-Liebler and Hellinger divergence measures, rejecting $H_0:\rho=0$ when the specified divergence measure exceeds some number d. This number d is so chosen that the resulting credible interval for the divergence measure has specified coverage probability $1-{\alpha}$. The length of such an interval is compared with the equal two-tailed credible interval and the HPD credible interval for $\rho$ with the same coverage probability which can also be inverted into acceptance regions of $H_0:\rho=0$. Example is considered where the HPD interval based on the one-at- a-time reference prior turns out to be the shortest credible interval having the same coverage probability.

• Journal of the Korean Mathematical Society
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• v.46 no.2
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• pp.363-447
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• 2009

The author previously defined the spectral invariants, denoted by $\rho(H;\;a)$, of a Hamiltonian function H as the mini-max value of the action functional ${\cal{A}}_H$ over the Novikov Floer cycles in the Floer homology class dual to the quantum cohomology class a. The spectrality axiom of the invariant $\rho(H;\;a)$ states that the mini-max value is a critical value of the action functional ${\cal{A}}_H$. The main purpose of the present paper is to prove this axiom for nondegenerate Hamiltonian functions in irrational symplectic manifolds (M, $\omega$). We also prove that the spectral invariant function ${\rho}_a$ : $H\;{\mapsto}\;\rho(H;\;a)$ can be pushed down to a continuous function defined on the universal (${\acute{e}}tale$) covering space $\widetilde{HAM}$(M, $\omega$) of the group Ham((M, $\omega$) of Hamiltonian diffeomorphisms on general (M, $\omega$). For a certain generic homotopy, which we call a Cerf homotopy ${\cal{H}}\;=\;\{H^s\}_{0{\leq}s{\leq}1}$ of Hamiltonians, the function ${\rho}_a\;{\circ}\;{\cal{H}}$ : $s\;{\mapsto}\;{\rho}(H^s;\;a)$ is piecewise smooth away from a countable subset of [0, 1] for each non-zero quantum cohomology class a. The proof of this nondegenerate spectrality relies on several new ingredients in the chain level Floer theory, which have their own independent interest: a structure theorem on the Cerf bifurcation diagram of the critical values of the action functionals associated to a generic one-parameter family of Hamiltonian functions, a general structure theorem and the handle sliding lemma of Novikov Floer cycles over such a family and a family version of new transversality statements involving the Floer chain map, and many others. We call this chain level Floer theory as a whole the Floer mini-max theory.

• Journal of the Korean Chemical Society
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• v.26 no.1
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• pp.1-6
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• 1982

The addition of thiophenol to ${\alpha}$-methylstyrene has been studied MO theoretically using CNDO/2 method. Although overall reaction proceeds in two steps i.e., (1) decomposition of thiophenol to give phenylthiyl radical and (2) addition of the radical to ${\alpha}$-methylstyrene to give a new monomer radical, theoretical results suggested that the phenylthiyl radical formation step, (1), was the dominant process in determining the rate of addition; this was the rationale behind the negative ${\rho}$ value obtained experimentally from the Hammett plots for substituents on the thiyl radicals. The departure from a linear Hammett plot for addition of ${\rho}$-chlorophenylthiyl and m-trifluoromethyl phenylthiyl to ${\rho}$-methoxy-${\alpha}$-methylstyrene could be explained as a result of an increased contribution of the addition step, (2) to the overall reaction rate.

• Parasites, Hosts and Diseases
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• v.52 no.3
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• pp.251-256
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• 2014

A novel recombinant Bacille Calmette-Guerin (rBCG) vaccine co-expressed Eimeria tenella rhomboid and cytokine chicken IL-2 (chIL-2) was constructed, and its efficacy against E. tenella challenge was observed. The rhomboid gene of E. tenella and chIL-2 gene were subcloned into integrative expression vector pMV361, producing vaccines rBCG pMV361-rho and pMV361-rho-IL2. Animal experiment via intranasal and subcutaneous route in chickens was carried out to evaluate the immune efficacy of the vaccines. The results indicated that these rBCG vaccines could obviously alleviate cacal lesions and oocyst output. Intranasal immunization with pMV361-rho and pMV361-rho-IL2 elicited better protective immunity against E. tenella than subcutaneous immunization. Splenocytes from chickens immunized with either rBCG pMV361-rho and pMV361-rho-IL2 had increased $CD4^+$ and $CD8^+$ cell production. Our data indicate recombinant BCG is able to impart partial protection against E. tenella challenge and co-expression of cytokine with antigen was an effective strategy to improve vaccine immunity.

• BMB Reports
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• v.44 no.6
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• pp.415-420
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• 2011

Diabetes is a well-known independent risk factor for vascular disease. However, its underlying mechanism remains unclear. It has been reported that increased influx of the hexosamine biosynthesis pathway (HBP) induces O-GlcNAcylation of proteins, leading to insulin resistance. In this study, we determined whether or not O-GlcNAc modification of proteins could increase vessel contraction. Using an endothelium-denuded aortic ring, we observed that glucosamine induced OGlcNAcylation of proteins and augmented vessel contraction stimulated by U46619, a thromboxane $A_2$ agonist, via augmentation of the phosphorylation of MLC20$MLC_{20}$, MYPT1(Thr855), and CPI17, but not phenylephrine. Pretreatment with OGT inhibitor significantly ameliorated glucosamine-induced vessel constriction. Glucosamine treatment also increased RhoA activity, which was also attenuated by OGT inhibitor. In conclusion, glucosamine, a product of glucose influx via the HBP in a diabetic state, increases vascular contraction, at least in part, through activation of the RhoA/Rho kinase pathway, which may be due to O-GlcNAcylation.

• Molecules and Cells
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• v.40 no.7
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• pp.495-502
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• 2017

The $5-HT_6R$ has been considered as an attractive therapeutic target in the brain due to its exclusive expression in the brain. However, the mechanistic linkage between $5-HT_6Rs$ and brain functions remains poorly understood. Here, we examined the effects of $5-HT_6R$-mediated cell morphological changes using immunocytochemistry, Western blot, and live-cell imaging assays. Our results showed that the activation of $5-HT_6Rs$ caused morphological changes and increased cell surface area in HEK293 cells expressing $5-HT_6Rs$. Treatment with 5-HT specifically increased RhoA-GTP activity without affecting other Rho family proteins, such as Rac1 and Cdc42. Furthermore, live-cell imaging in hippocampal neurons revealed that activation of $5-HT_6Rs$ using a selective agonist, ST1936, increased the density and size of dendritic protrusions along with the activation of RhoA-GTP activity and that both effects were blocked by pretreatment with a selective $5-HT_6R$ antagonist, SB258585. Taken together, our results show that $5-HT_6R$ plays an important role in the regulation of cell morphology via a RhoA-dependent pathway in mammalian cell lines and primary neurons.

• Polymer(Korea)
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• v.34 no.4
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• pp.346-351
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• 2010

The mechanical properties of polypropylene (PP) composites, especially the effects of the filler shapes on the modulus were studied. The experimental results were investigated and compared with the theoretical approaches proposed by Lee and Paul and based on Eshelby's principle, which three dimensional ellipsoids were filled as filler and analyzed in terms of aspect ratio, ${\rho}_\alpha=a_1/a_3$ and ${\rho}_\beta=a_1/a_2$. The shapes of fillers were observed by SEM and aspect ratios were statistically calculated. Young's moduli in the longitudinal and transverse directions for barium sulfate whose shape was sphere ($\rho_\alpha=\rho_\beta=1$) had the same values, as predicted values. The modulus in the $x_1$ direction for a glass fibers increased as the filler content increased, while the modulus in the $x_3$ direction was increased relatively small. Furthermore, mica was also used to investigate the effects of the primary and secondary aspect ratios on the mechanical properties.

• Polymer(Korea)
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• v.34 no.4
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• pp.352-356
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• 2010

The effects of the filler shapes and contents on the coefficient of thermal expansion (CTE) for polypropylene (PP) composites which included three dimensional ellipsoids ($a_1>a_2>a_3$), as determined by two aspect ratios (${\rho}_\alpha=a_1/a_3$ and ${\rho}_\beta=a_1/a_2$) were analyzed by the theoretical approach proposed by Lee and Paul and compared with the experimental results. The shapes of fillers in the composites were various, such as spherical, fiber, disc, and ellipsoid, using barium sulfate, glass fiber, and mica. The longitudinal CTE of barium sulfate whose shape was sphere ($\rho_\alpha=\rho_\beta=1$) decreased. For the glass fiber, primary aspect ratio decreased with the filler content, and longitudinal CTE decreased as filler contents increased. Normal CTE initially increased in the lower filler content. For the mica, longitudinal and transverse CTE decreased but normal CTE increased in the lower filler content like predicted values.

• Molecules and Cells
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• v.44 no.7
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• pp.458-467
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• 2021

GPR43 (also known as FFAR2 or FFA2) is a G-protein-coupled receptor primarily expressed in immune cells, enteroendocrine cells and adipocytes that recognizes short-chain fatty acids, such as acetate, propionate, and butyrate, likely to be implicated in innate immunity and host energy homeostasis. Activated GPR43 suppresses the cAMP level and induces Ca²⁺ flux via coupling to Gα_i and Gα_q families, respectively. Additionally, GPR43 is reported to facilitate phosphorylation of ERK through G-protein-dependent pathways and interacts with β-arrestin 2 to inhibit NF-κB signaling. However, other G-protein-dependent and independent signaling pathways involving GPR43 remain to be established. Here, we have demonstrated that GPR43 augments Rho GTPase signaling. Acetate and a synthetic agonist effectively activated RhoA and stabilized YAP/TAZ transcriptional coactivators through interactions of GPR43 with Gα_q/11 and Gα_12/13. Acetate-induced nuclear accumulation of YAP was blocked by a GPR43-specific inverse agonist. The target genes induced by YAP/TAZ were further regulated by GPR43. Moreover, in THP-1-derived M1-like macrophage cells, the Rho-YAP/TAZ pathway was activated by acetate and a synthetic agonist. Our collective findings suggest that GPR43 acts as a mediator of the Rho-YAP/TAZ pathway.

• The Korean Journal of Physiology and Pharmacology
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• v.27 no.4
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• pp.325-331
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• 2023

α₁-adrenoceptors link via the G-protein Gq/G₁₁ to both Ca²⁺ entry and release from stores, but may also activate Rho kinase, which causes calcium sensitization. This study aimed to identify the subtype(s) of α₁-adrenoceptor involved in Rho kinase-mediated responses in both rat aorta and mouse spleen, tissues in which contractions involve multiple subtypes of α₁-adrenoceptor. Tissues were contracted with cumulative concentrations of noradrenaline (NA) in 0.5 log unit increments, before and in the presence of an antagonist or vehicle. Contractions produced by NA in rat aorta are entirely α₁-adrenoceptor mediated as they are competitively blocked by prazosin. The α_1A-adrenoceptor antagonist RS100329 had low potency in rat aorta. The α_1D-adrenoceptor antagonist BMY7378 antagonized contractions in rat aorta in a biphasic manner: low concentrations blocking α_1D-adrenoceptors and high concentrations blocking α_1B-adrenoceptors. The Rho kinase inhibitor fasudil (10 µM) significantly reduced aortic contractions in terms of maximum response, suggesting inhibition of α_1B-adrenoceptor mediated responses. In the mouse spleen, a tissue in which all 3 subtypes of α₁-adrenoceptor are involved in contractions to NA, fasudil (3 µM) significantly reduced both early and late components to the NA contraction, the early component involving α_1B- and α_1D-adrenoceptors, and the late component involving α_1B- and α_1A-adrenoceptors. This suggests that fasudil inhibits α_1B-adrenoceptor mediated responses. It is concluded that α_1D- and α_1B-adrenoceptors interact in rat aorta and α_1D-, α_1A- and α_1B-adrenoceptors interact in the mouse spleen to produce contractions and these interactions suggest that one of the receptors preferentially activates Rho kinase, most likely the α_1B-adrenoceptor.