• Applied Microscopy
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• v.36 no.3
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• pp.227-234
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• 2006

To investigate the comparative effect of spherical and aspherical RGP lens were worn during 3 weeks on rabbit's cornea. Four white rabbits were worn right eyes with spherical lens and 4 white rabbits were worn right eyes with aspherical RGP lens. Left eyes were served as control. The rabbits were sacrificed at 3 weeks after fitting and observed morphological changes by scanning electron microscopy and also investigate proliferation rate of the corneal epithelium with RGP wearing. After spherical RGP lens wearing, the epithet layer damaged compared to aspherical lens. The superficial cell layer strip off seriously, cell size significantly changed abnormal. Both spherical and aspherical RGP lens fitting group showed so many bacteria and back surface of lens was found like a fern shape. The aspherical RGP lens original material type was some formal than spherical lens. We thought that these pattern was significantly altered with spherical lens by prohibited transmitter oxygen from atmosphere therefore the epithelium shape was changed. This suggested wearing the aspherical lens might be less physiologic than shperical lens fitting.

• Journal of Korean Ophthalmic Optics Society
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• v.13 no.4
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• pp.43-49
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• 2008

Purpose: The current study was conducted to evaluate the compatibility of UV-A blocking contact lens on eye protection with regular contact lens. Methods: The protective activity of regular contact lens (UV-A blocking: 20%) and UV-A blocking contact lens (UV-A blocking: 85%) on the denaturation of RNase A, catalase, and superoxide dismutase (SOD) induced UV-A irradiation were compared by acrylamide gel electrophoresis. The enzyme solutions were irradiated with UV-A for 1, 3, 6, 24 and 96 hours at the wavelength of 365 nm. Covering area with contact lenses were varied as 50%, 70% and 100% according to the calculation of blocking areas of anterior eye that could be covered with RGP lens, soft contact lens, and eye glasses, respectively. Results: Denaturations of RNase, catalase and SOD were exaggerated when they were exposed to UV-A for a longer period. The denaturation was effectively prevented by UV-A blocking contact lens compared to regular contact lens. The capability of UV-A blocking contact lens was considerably reduced when the covering area with contact lens decreased and exposure time to UV-A extended. Conclusion: Therefore, it would be suggested that wearing contact lens for a long time under sunlight is carefully considered since the activity of UV-A blocking contact lens against UV-A irradiation may not be enough to protect enzymes presented in eyes when exposure time to UV-A increased.

• Journal of Korean Ophthalmic Optics Society
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• v.20 no.2
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• pp.237-246
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• 2015

Purpose: The present study was conducted to reveal the correlation of structural denaturation and decrease of enzyme activity when the antioxidative enzymes, superoxide dismutase (SOD) and catalase (CAT) were repeatedly exposed to UV-B, and further investigate whether the denaturation and inactivation of those enzymes can be effectively blocked by using UV-inhibitory RGP lens. Methods: Each enzyme solution was prepared from the standardized SOD and CAT, and repeatedly exposed to UV-B of 312 nm for 30 minutes, 1 hour and 2 hours a day over 1, 2, 3, 4 and 5 days. Structural denaturation of SOD and CAT induced by repeat UV-B irradiation was confirmed by the electrophoretic analysis, and their enzyme activity was determined by the colorimetric assay using the proper assay kit. At that time, the change in structure and activity of the antioxidant enzymes directly exposed to UV-B was compared to the case that UV-B was blocked by UV-inhibitory RGP lens. Results: SOD exposed repeatedly to UV-B showed the polymerization pattern in the electrophoretic analysis when it repeatedly exposed for 30 min a day, however, the change of its activity was less than 10%. On the other hand, CAT repeatedly exposed to UV-B reduced size and density of the electrophoretic band which indicated a structure denaturation, and its activity was significantly decreased. In the case that the repeat exposure time was longer, CAT activity was completely lost even though some enzyme band occurred in the electrphoretic analysis. In addition, the degeneration of CAT due to UV-B irradiation was inhibited to some extent by using RGP lens with a UV-B blocking of 63.7%, however, it was not completely inhibited. Conclusions: From these results, it was revealed that the structural denaturation of antioxidative enzymes was not perfectly correlated with the reduction in enzyme activity according to the type of enzyme. It is recommended to minimize the exposure time to UV when wearing contact lens, or wear the contact lenses having UV blocking rate of the FDA Class I blocker or the sunglasses having equivalent UV-blocking rate for reducing the damage of antioxidative enzymes induced by UV.

• Journal of Korean Ophthalmic Optics Society
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• v.11 no.3
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• pp.181-185
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• 2006

The measurement of the radius of corneal curvature with keratometer was followed in 184 university students who wearing RGP contact lens and consisted of female(167), male(17) and keratoconus patients(3). Overall mean value in the radius of corneal curvature is 7.77mm, and right and left eyes is appeared the same mean value. Overall mean value in horizontal and vertical is 7.88mm and 7.65mm. Horizontal means is larger than vertical means by 0.22mm of the all female and male students. Male's mean value in the radius of corneal curvature(7.84mm) is larger than female's by 0.08mm, and right and left eyes is also the same mean value. Keratoconus patients' mean value in the radius of corneal curvature(6.86mm) is smaller than others students by 0.91mm.

• Journal of Korean Ophthalmic Optics Society
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• v.18 no.2
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• pp.197-202
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• 2013

Purpose: This research was performed to measure and analyze scotopic pupil size in myopes and to figure out the factors that influence it. Methods: The pupil size of 191 healthy myopic subjects were measured with the pupillometer (Colvard pupillometer, OASIS medical, USA) in scotopic and analyzed with the age, corneal size, spherical equivalent refractive error, corneal curvature. In addition, it was compared with the measurements of intra-examiner and inter-examiner to verify reproducibility of pupillometer. Results: The mean (${\pm}$SD) scotopic pupil size was $6.64{\pm}0.68$ mm (range, 5.00~8.00 mm), the lower age and the larger corneal size, The bigger the pupil size. The lower spherical equivalent refractive error and steepper corneal curvature tends to be smaller. The reproducibility of intra-examiner and inter-examiner in pupillometer showed the reliability highly (Guttman splithalf point > 0.91). Conclusions: The pupil size associated with age, corneal size, spherical equivalent refractive error and corneal curvature in scotopic condition. It can refer to prevent inconvenience that may occur RGP contact lenses, cataract surgery and refractive surgery.

• Journal of Korean Ophthalmic Optics Society
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• v.17 no.4
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• pp.469-476
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• 2012

Purpose: The purpose of this study is to investigate the changes of asphericity according to diameter and direction of the cornea. Methods: The changes of asphericity according to diameter and direction of cornea is measured by using Pentacam(Oculus Co., Germany), as targeting 57 adults (average $27.0{\pm}4.8$, 20 men, 37 women) without any ophthalmic diseases, which may be effective in eyesight, and refractive surgery. Results: As diameter increases in every direction based on the corneal vertex, asphericity has attentively increased (p<0.05), and the size of asphericity in each direction from every measured diameter showed as superior > nasal > inferior > temporal. In group comparison by nearsightness and astigmatism level, asphericity measured high when nearsightness and astigmatism levels were higher, and this appears to be statistically attentive.(p<0.05). Conclusions: Asphericity of cornea significantly increased according to increase of diameter of cornea, and as measured figure or increased quantity appeared differently in all directions based on corneal vertex, so it helps to understand shape of cornea, and considered that is the part to be considered during manufacturing and fitting of RGP lenses.