Browse > Article

Spherical and Aspherical RGP Lens Fitting to Epithelium and Endothelium of Rabbit's Cornea -Scanning Electron Microscopy  

Kim, In-Suk (Department of Ophthalmic Optics of Chodang University)
Ryu, Gun-Chang (Department of Biology, Chonnam National University)
Chae, Soo-Chul (Department of Biology, Chonnam National University)
Jeon, Chang-Jin (Department of Biology, Kyungpook National University)
Publication Information
Applied Microscopy / v.36, no.3, 2006 , pp. 227-234 More about this Journal
Abstract
To investigate the comparative effect of spherical and aspherical RGP lens were worn during 3 weeks on rabbit's cornea. Four white rabbits were worn right eyes with spherical lens and 4 white rabbits were worn right eyes with aspherical RGP lens. Left eyes were served as control. The rabbits were sacrificed at 3 weeks after fitting and observed morphological changes by scanning electron microscopy and also investigate proliferation rate of the corneal epithelium with RGP wearing. After spherical RGP lens wearing, the epithet layer damaged compared to aspherical lens. The superficial cell layer strip off seriously, cell size significantly changed abnormal. Both spherical and aspherical RGP lens fitting group showed so many bacteria and back surface of lens was found like a fern shape. The aspherical RGP lens original material type was some formal than spherical lens. We thought that these pattern was significantly altered with spherical lens by prohibited transmitter oxygen from atmosphere therefore the epithelium shape was changed. This suggested wearing the aspherical lens might be less physiologic than shperical lens fitting.
Keywords
BrdU lebelled; Epithelium and endothelium of rabbit's cornea; Spherical and aspherical RGP lens;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Fischbarg J, Lim JJ: Fluid and electrolyte transports across corneal endothelium, In Current Topics in Eye Research, Vol 4 (Ed. Zadunaisky JA, Davson H), Academic Press, New York 201-223, 1984
2 Holden BA, Mertz GW: Critical oxygen Levels to corneal edema for daily and extended wear contact lens, Invest Ophthalmol Vis Sci 25 : 1161-1167, 1984
3 Holden BA: The ocular response to contact lens wear, Optom Vis Sci 66 : 717-733, 1990
4 Ladage PM, Yamamoto K, Ren DH, et al.: Effects of rigid and soft contact lens daily wear on corneal epithelium, tear lactate dehydrogenase and bacterial binding to exofoliated epithelial cells, Ophthalmology 108 : 2001
5 Ladage PM, Yamamoto K, Ren DH, et al.: Proliferation rate of rabbit corneal epithelium during overnight rigid contact lens wear, Invest Ophthalmol vis Sci 42 : 2804-2812, 2001
6 Maurice DM: The cornea and the sclera: Invest Physiology of the eye (Ed. Davson, H), Academic Press, Orlando 1-158, 1984
7 Madigan MC, Holden BA: Reduced epithelial adhesion after extended contact lens wear correlates with reduced hemidesmosome density in cat cornea, Invest Ophthalmol Vis Sci 33 : 314-323, 1992
8 O'Leary DJ, Madgewick R, Wallace J, Ang J: Size and number of epithelial cells washed form the cornea after contact lens wear, Optom Vis Sci 692-696, 1998   DOI
9 Lemp MA, Gold JB: The effect of extended-wear hydrophilic contact lens on the human corneal epithelium, Am J Ophthalmol 101 : 274-277, 1986   DOI
10 Hodson SA, Guggenheim J, Kaila D, Wiogham C: Anion pumps in ocular tissues, Biochem soc trans 19 : 849-852, 1991   DOI
11 Gobbles M, Breitbach R, Spitznas M: Barrier function of the corneal epithelium of contact lens patients. Forschr Ophthalmol 87 : 646-648, 1990
12 Zantos SG, Holden BA: Transient endothelial changes soon after wearing soft contact lenses, Am J Optom Physiol Opt 54 : 856-858, 1977   DOI
13 Ilhwon Kwak, joohwa Lee, myungjin Ju: Cell apoptosis induced by contact lens fitting of rabbit's cornea, J KOS 39 : 2591-2597, 1998
14 Bruce AS, Brennan NA: Corneal pathophisiology with contact lens wear, Sur Ophthalmol 25-58, 1990
15 Ren DH, Petroll WM, Jester JV, Cavanagh HD: The effect of rigid gas permeable contact lens wear on proliferation of rabbit corneal and conjugatival epithelial cell, CLAO J 25 : 136-141, 1999