• Journal of the Korea Society of Computer and Information
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• v.27 no.12
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• pp.9-16
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• 2022

In this paper, we propose a plan to design and utilize the RDBS(Record Block Data file management System) so that data can be recovered when data files in the Combat Management System are mismatched. The CMS(Combat Management System) manages the same files in multiple IPN(Infomation Processing Node) repositories to support multiplexing. However, mismatches in data files can occur due to equipment maintenance or user immaturity. The existing CMS does not manage the history of changes in data files, and when a mismatch occurs, data file were synchronized based on the latest date. But, It is difficult to say that files with the latest date have the highest reliability, and once the file synchronization has progressed, it cannot be recovered with pre-synchronization data. To solve this problem, data was stored and synchronized in units of record blocks using RDBS proposed in this paper, and the Rsync algorithm was used to reduce the overhead of file synchronization due to units of record blocks. SW applied with RDBS was tested for performance in a simulated environment, and it was confirmed that it could be applied to CMS through normal operation confirmation.

• Reproductive and Developmental Biology
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• v.37 no.3
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• pp.109-115
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• 2013

Until recently the most popular tetracycline-inducible gene expression system has been the one developed by Gossen and Bujard. In this study, we tested the latest version of same system and the results are summarized as follows: Compared with previous one, the difference of new system are minor changes of nucleotide sequences in transactivator and tetracycline response element (TRE) regions. Sensitivity to the doxycycline (a tetracycline derivative) was improved. Leakiness of GFP marker gene expression in non-inducible condition was significantly decreased. Higher expression of the marker gene was observed when the cells were fed with doxycycline-containing medium. Optimal insertion site of woodchuck posttranscriptional regulatory element (WPRE) sequence which was known to increase gene expression was different depending on the origin of cells. In chicken embryonic fibroblast, location of WPRE sequence at 3' end of TRE resulted in the highest GFP expression. In bovine embryonic fibroblasts, 3' end of transactivator was the best site for the GFP expression.

• Reproductive and Developmental Biology
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• v.37 no.3
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• pp.91-96
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• 2013

Transgenic chickens have been spotlighted as an highly potent bioreactor for their fecundity, short generation time, and eggs associated with mass production of protein. In this study, we generated transgenic chickens exhibiting oviduct specific expression of human growth hormone fused to human transferrin for oral administration. Gene of the modified growth hormone located at downstream ovalbumin promoter (~3.6 kb) was introduced to stage X blastodermal cell employing retrovirus vector system. Several transgenic chickens were successfully generated. However, genomic analyses showed unexpected deletion within the transgene. The modification of the transgene seemed to occur during germ cell formation because the deletion was detected only from the sperm DNA of the G0 founder animal. There was no evidence of deletion in the somatic cell DNA samples of the same chicken. Consequently, same pattern of the deletion was confirmed in both somatic and germ cells of the G1 progeny.

• Reproductive and Developmental Biology
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• v.38 no.4
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• pp.143-146
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• 2014

Antioxidants, as reactive oxygen species scavengers, are one of the beneficial additives in serum-free defined culture medium. In this study, three separate experiments were performed to determine the effects of 3-hyroxyflavone added to the culture medium on the developmental competence of follicular bovine oocytes during in vitro maturation (IVM) and/or in vitro culture (IVC). The rate of blastocyst developed from oocytes cultured in IVM medium with 3-hyroxyflavone was significantly higher than that from control oocytes (39.0% vs. 26.3%, p<0.001), respectively. However, oocytes cultured in the medium with addition of 3-hyroxyflavone only at IVC period did not show significance in the blastocyst development when compared with control. When 3-hyroxyflavone was added to both IVM and IVC media, the rate of blastocyst formation was even significantly lower (21.1%) than control (26.5%; p<0.05). The present findings suggested that antioxidative activity of 3-hydroxyflavone added to only IVM medium beneficially affected the developmental competence of follicular bovine.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 2004.05a
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• pp.9-12
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• 2004

Developing an efficient knowledge base construction mechanism as an input method for expert systems (ES) development is of extreme importance due to the fact that an input process takes a lot of time and cost in constructing an ES. Most ES require experts to explicit their tacit knowledge into a form of explicit knowledge base with a full sentence. In addition, the explicit knowledge bases were composed of strict grammar and keywords. To overcome these limitations, this paper proposes a knowledge conceptualization and construction mechanism for automated knowledge acquisition, allowing an efficient decision. To this purpose, we extended traditional knowledge map (KM) construction process to dynamic knowledge map (DKM) and combined this algorithm with relational database (RDB). In the experiment section, we used medical data to show the efficiency of our proposed mechanism. Each rule in the DKM was characterized by the name of disease, clinical attributes and their treatments. Experimental results with various disease show that the proposed system is superior in terms of understanding and convenience of use.

• The Journal of Society for e-Business Studies
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• v.8 no.1
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• pp.85-102
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• 2003

Nowadays an information exchange on XML such as B2B electronic commerce is spreading. Therefore the systematic and stable management mechanism for storing the exchanged information is needed. For this goal there are many research activities for connection between XML application and relational database. But because XML data have hierarchical structures and relational database can store only flat-structured data, we need to make the conversion rule which changes the hierarchical architecture to 2-dimensional information. Accordingly the modeling methodology for storing each structured information in relational database is needed. In this paper, we introduce a XML modeling methodology to design W3C XML schema using UML and we propose a unified design methodology for relational database schema to store XML data efficiently in relational databases. In the second place, in order to verify objectivity of a unified design methodology. By the way of Ronald Bourret, First we introduce the method of the transformation from XML schema to object model and second we translate object model into relational database schema. Therefore we show the mutual consistency between those consequence, and so can verify a unified design methodology.

• IE interfaces
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• v.17 no.3
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• pp.330-337
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• 2004

Since XML (eXtensible Markup Language) was highlighted as an information interchange format, there is an increasing demand for incorporating XML with databases. Most of the approaches are focused on RDB (Relational Databases) because of legacy systems. But these approaches depend on the database system. Countless researches are being focused on DTD (Document Type Definition). However XML Schema is more comprehensive and efficient in many perspectives. We propose a meta-model for XML Schema that is independent of the database. There are three processes to build our meta-model: DOM (Document Object Model) tree analysis, object modeling and storing object into a fixed DB schema using model mapping approach. We propose four mapping rules for object modeling, which conform to the ODMG (Object Data Management Group) 3.0 standard. We expect that the model will be especially useful in building XML-based e-business applications.

• Reproductive and Developmental Biology
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• v.40 no.1
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• pp.7-13
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• 2016

Effectiveness of transgene transfer into genome is crucially concerned in mass production of the bio-pharmaceuticals using genetically modified transgenic animals as a bioreactor. Recently, the mammary gland has been considered as a potential bioreactor for the mass production of the bio-pharmaceuticals, which appears to be capable of appropriate post-translational modifications of recombinant proteins. The mammary gland tissue specific vector system may be helpful in solving serious physiological disturbance problems which have been a major obstacle in successful production of transgenic animals. In this study, to minimize physiological disturbance caused by constitutive over-expression of the exogenous gene, we constructed new retrovirus vector system designed for mammary gland-specific expression of the hEPO gene. Using piggyBac vector system, we designed to express hEPO gene under the control of mammary gland tissue specific and lactogenic hormonal inducible goat ${\beta}$-casein or mouse Whey Acidic Protein (mWAP) promoter. Inducible expression of the hEPO gene was confirmed using RT-PCR and ELISA in the mouse mammary gland cells treated with lactogenic hormone. We expect the vector system may optimize production efficiency of transgenic animal and reduce the risk of global expression of transgene.

• Reproductive and Developmental Biology
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• v.40 no.1
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• pp.1-5
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• 2016

This study investigated the effects of L-carnitine (LC) and nicotinic acid (NA) on sperm viability during liquid storage at $18^{\circ}C$ in miniature pigs. $10{\mu}M$ LC and 30 mM NA, combined LC and NA (LN) were treated in fresh semen for 3, 7, and 10 days. In results, sperm survival increased in NA- and LN-treated semen on 7 and 10 days (p<0.05), mitochondrial integrity of live sperm increased in LN-treated semen on 7 days (p<0.05), but not NA-treated semen. In addition, we examined the acrosome reaction of sperm in miniature pigs. LC and NA did not influence on acrosome reaction of boar sperm. In conclusion, LC and NA effectively maintained the viability and quality of sperm during long-term storage in miniature pigs, suggesting that the combined LN may be useful for improving the semen extender for long-term liquid storage in pigs.

• Reproductive and Developmental Biology
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• v.38 no.1
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• pp.41-52
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• 2014

Embryonic genome activation (EGA) is a highly complex phenomenon that is controlled at various levels. New studies have ascertained some molecular mechanisms that control EGA in several species; it is apparent that these same mechanisms regulate EGA in all species. Protein phosphorylation, DNA methylation and histone modification regulate transcriptional activities, and mechanisms such as ubiquitination, SUMOylation and microRNAs post-transcriptionally regulate development. Each of these regulations is highly dynamic in the early embryo. A better understanding of these regulatory strategies can provide the possibility to improve the reproductive properties in mammals such as pigs, to develop methods of generating high-quality embryos in vitro, and to find markers for selecting developmentally competent embryos.