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http://dx.doi.org/10.12749/RDB.2016.40.1.7

In Vitro Assay of Mammary Gland Tissue Specific hEPO Gene Expression  

Koo, Bon Chul (Department of Physiology, Catholic University of Daegu School of Medicine)
Kwon, Mo Sun (Department of Physiology, Catholic University of Daegu School of Medicine)
Kim, Teoan (Department of Physiology, Catholic University of Daegu School of Medicine)
Publication Information
Reproductive and Developmental Biology / v.40, no.1, 2016 , pp. 7-13 More about this Journal
Abstract
Effectiveness of transgene transfer into genome is crucially concerned in mass production of the bio-pharmaceuticals using genetically modified transgenic animals as a bioreactor. Recently, the mammary gland has been considered as a potential bioreactor for the mass production of the bio-pharmaceuticals, which appears to be capable of appropriate post-translational modifications of recombinant proteins. The mammary gland tissue specific vector system may be helpful in solving serious physiological disturbance problems which have been a major obstacle in successful production of transgenic animals. In this study, to minimize physiological disturbance caused by constitutive over-expression of the exogenous gene, we constructed new retrovirus vector system designed for mammary gland-specific expression of the hEPO gene. Using piggyBac vector system, we designed to express hEPO gene under the control of mammary gland tissue specific and lactogenic hormonal inducible goat ${\beta}$-casein or mouse Whey Acidic Protein (mWAP) promoter. Inducible expression of the hEPO gene was confirmed using RT-PCR and ELISA in the mouse mammary gland cells treated with lactogenic hormone. We expect the vector system may optimize production efficiency of transgenic animal and reduce the risk of global expression of transgene.
Keywords
Human Erythropoietin (hEPO); Mammary Gland Tissue Specific Gene Expression; ${\beta}$-Casein Promoter; WAP Promoter;
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