• Title/Summary/Keyword: RDA test

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Effect of Dietary Herb Extract Mix on Antioxidative Activity of Chicken Thigh Meat (약용식물 추출물 급여가 닭 다리육의 항산화 기능에 미치는 영향)

  • Jo, Cheor-Un;Jang, Ae-Ra;Jung, Samooel;Choe, Jun-Ho;Kim, Bin-Na;Lee, Kyong-Haeng
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.3
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    • pp.302-308
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    • 2009
  • This study was performed to investigate the effect of dietary medicinal herb extract mix (MHEM, mulberry leaf : Japanese honeysuckle : goldthread=48.5:48.5:3.0) on antioxidative activity of chicken thigh meat. The dietary treatments consisted of a corn-soybean meal basal diet (control), basal diet with 0.3% and 1% MHEM. At the end of the feeding trial, thigh meat samples were collected and stored in a refrigerator at $4^{\circ}C$ to be analyzed on day 0, 3, 7 and 14. The MHEM did not affect proximate composition of the thigh meat. Total phenol contents of the thigh meats in treatment groups were higher than that of the control (p<0.05). 1,1-Diphenyl-2-picrylhydrazyl radical scavenging activity were higher in dietary treatment group. 2-Thiobarbituric acid reactive substances values of the treatment group were lower than that of control at day 7 and 14 and the added level also affected the inhibition of lipid oxidation of thigh meat. In sensory test, panelists preferred treatment group at day 7 and 14. Therefore, results indicate that dietary MHEM added to chicken may retard the oxidative deterioration of chicken thigh meat during cold storage.

Isolation and Characterization of Plant Growth Promoting Rhizobacterium Bacillus subtilis YK-5 from Soil (토양에서 식물생육촉진 활성을 가진 균주 Bacillus subtilis YK-5의 분리 및 특성)

  • Yeo, Soo-Hwan;Yook, Young-Min;Kim, Hyun-Soo
    • KSBB Journal
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    • v.24 no.4
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    • pp.334-340
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    • 2009
  • For the isolation of a plant growth-promoting rhizobacteria, strain YK-5 was selected from approximately 400 thermostable strains isolated from special soil samples. Strain YK-5 produced an antifungal compound, and optimum carbon and nitrogen sources for the production of the antifungal compound were investigated against Aspergillus flavus as a test strain. Modified LB medium containing 1% peptone, 1% yeast extract and 5% black sugar was determined to be the optimal medium for growth and antifungal compound production. Culture broth of strain YK-5 potently inhibited growth of the phytopathogenic fungus Fusarium oxysporum KACC 40052 for 7 days. The plant growth-promotion function of strain YK-5 was tested against radish and rice in pot trials. Leaf number, plant height and root length in YK-5-treated radish markedly exceeded (> 60%) those of untreated radish. Leaf length and white rootlet development were markedly more prominent than in commercially-treated rice plants. Strain YK-5 was determined to be Bacillus subtilis YK-5 by physiological, chemotaxonomical, and phylogenetical analyses.

A Study on Fast Food Consumption, Nutritional Knowledge, Food Behavior and Dietary Intake of University Students (대학생의 패스트푸드 이용, 영양지식, 식행동, 영양소 섭취 실태에 관한 연구)

  • Kim, Gyeong-Won;Sin, Eun-Mi;Mun, Eun-Hye
    • Journal of the Korean Dietetic Association
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    • v.10 no.1
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    • pp.13-24
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    • 2004
  • This study was designed to examine fast food consumption, nutritional knowledge, food behavior and dietary intake of university students and to investigate if there were differences in these variables by fast food consumption. The questionnaire was administered to university students in Daejeon. Data(n=269) was analyzed using $x^2$-test and analysis of variance. Subjects were mostly female(62%) and freshmen or sophomores(86%). Based on the frequency of fast food consumption, subjects were categorized into non-users(27.9%), users(<2 times/week, 42%) and frequent users($\geq$2 times/week, 30.1%). Those who used fast foods(n=194) consumed the foods 7.5 times per month, on the average. Subjects scored 15.6 out of 20 on a nutritional knowledge scale, showing the moderate level of knowledge. When examined by fast food use, the nutritional knowledge score was 15.5 for non-users, 16.1 for users, and 15.0 for frequent-users(p<0.05). Only two items, regarding 'fat type(animal, plant) and health' and 'importance of having breakfast', were significantly different by fast food consumption, with user group and non-users scoring higher than frequent-users(p<0.05). Food behaviors, measured by 20 items, were not desirable, with mean scores of 51.5(possible score: 20-100). Subjects showed problems in eating meals regularly, eating a variety of foods, eating breakfast, and consumption of some food groups(vegetables, fruits, and proteins). Fast food non-users showed more desirable food behaviors than users or frequent-users, such as having processed foods (p<0.001) and eating-out less frequently(p<0.01). Dietary intake data showed that some nutrient intakes, including energy, calcium, iron, zinc and folic acid were less than 75% of the RDA. Index of nutritional quality(INQ) was adequate except for calcium and zinc. Compared to non-users or user group, frequent-users of fast foods consumed higher amounts of lipids(p<0.05), and had lower INQ for calcium(p<0.01). This study described the status of fast food consumption, nutritional knowledge, food behavior of university students, and provided some baseline data for planning nutrition education for university students.

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Efficacy Test of Mating Disruptors Against Peach Fruit Moth, Grapholita molesta, using Polypropylene Dispenser Containing Ester Wax (에스테르 왁스가 함유된 폴리프로필렌 방출기 제조와 복숭아순나방 교미교란 효과 검정)

  • Yoon, Ji Young;Kim, Da Eun;Im, Yu Na;Lee, Ji Sung;Yang, Chang Yeol;Kim, Jong-Duk
    • Korean journal of applied entomology
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    • v.54 no.4
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    • pp.369-374
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    • 2015
  • Grapholita molesta (GM) has been considered as a major problem to apple and peach orchards. Mating disrupters have been applied to control the pest over the world as an Integrated Pest Management [IPM] tool. Various types of dispensers releasing mating disrupters have been developed with different formulation of wax, polyethylene tube and aerosol. In this study, the dispensers that were composed of ester waxes and polypropylene (PP) film were used as dispenser materials to analyze release pattern of GM pheromone compared with paraffin wax and polyethylene (PE) film. The release pattern in PP film group was consistent with time while PE film group irregular. Based on these results, Japan wax_PP film dispenser (Japan_PP) was manufactured and showed the mating disruption effect of 98% during 5months in peach orchard.

Influence of Silicate Application on Rice Grain Quality (규산 시용이 미입의 품질에 미치는 영향)

  • Kang, Yang-Soon;Lee, Jong-Hoon;Kim, Jeong-Ill;Lee, Jae-Saeng
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.6
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    • pp.800-804
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    • 1997
  • To evaluate the characters related to rice grain quality as affected by silicate application, the ripened color, appearence and physicochemical properties of rice grain which were produced at the silt-loam paddy field of National Yeongnam Agricultural Exeperiment Station with a little poor drainage for trials on long-term effects of continuous application of same fertilizer were analyzed comparing with the NPK fertilizer an the NPK+compost in 1995. The whiteness value and color space value in ripened color of rice hull by silicate application were higher than those in the plots of NPK and NPK+compost, because of low percentage of rusty grain and sooty grain, The percentage of perfect grain in brown rice and in milled rice by the silicate application increased by 6.1~7.5% and by 1.8~3.5% respectively, as compared with the NPK application and white core and white belly of milled rice were decreased, The amylose content, protein content and starch-iodine blue value of milled rice by silicate application were lower than those in the plots of NPK and NPK+compost, while the peak point and breakdown viscosity of gelatinized rice flour on amylograph, and tastes value evaluated as a indicater of pannel test by nireco tester were higher. By silicate application these factors could be affected toward better in eating quality.

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Effects of Fly Ash and Gypsum Mixture on Reducing Phosphorus Loss from Paddy Soil (논 토양에서 석탄회와 석고의 혼합제를 활용한 인산유출 저감)

  • Lee, Yong-Bok;Lee, Seul-Bi;Oh, Ju-Hwan;Lee, Chang-Hoon;Hong, Chang-Oh;Kim, Pil-Joo
    • Korean Journal of Environmental Agriculture
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    • v.27 no.1
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    • pp.66-71
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    • 2008
  • Phosphorus transfer from agricultural soils to surface waters is an important environmental issue. Fly ash and phospho-gypsum which are industrial by-product were investigated as a means of reducing dissolved phosphorus in arable soil. To determine the optimum mixing ratio of fly ash(FA) and phospho-gypsum(PG) for reducing dissolved reactive P(DRP) in soil, various mixture ratio of FA and PG were mixed with two soil. The DRP content and pH in soils were analysed after 3 weeks incubation under flooding condition. Although DRP content in soils was significantly decreased by FA-PG mixture compared with control, there were no significant difference among the FA and PG mixture ratio of 75:25, 50:50, and 25:75. The mixture of 75% FA and 25% PG was selected for field test. A field experiment was carried out to evaluate the reducing DRP content in paddy soil to which 0(NPK), 20(FG 20), 40(FG 40), and 60(FG 60) Mg $ha^{-1}$ of the mixture were applied. The DRP content was reduced by 31% at the application rate of 60 Mg $ha^{-1}$. In contrast to deceasing DRP, Ca-P content increased significantly with the mixture application rate. After rice harvesting, available $SiO_2$, P, and exchangeable Ca content in soil increased significantly with application rate due to high content of Si, P, and Ca in the mixture. Mixtures of fly ash and gypsum should reduce P loss from paddy soil and increase soil fertility.

Calcium current on cryopreservation in mouse oocytes (동결 생쥐 난자에서의 calcium 전류)

  • Kang, Da-Won;Kim, Eun-Sim;Choe, Chang-Yong;Park, Jae-Yong;Han, Jae-Hee;Hong, Seong-Geun
    • Korean Journal of Veterinary Research
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    • v.42 no.1
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    • pp.35-43
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    • 2002
  • Cryopreservation is commonly used for an efficient utilization of semen, oocytes and embryos but has disadvantage in the survival, development of the post-thawed eggs. The high risk in the survival, development of eggs after thawing is thought to be caused by inappropriate internal regulation of $Ca^{2+}$ and/or formation of intracellular ice crystals. In this experiment, we tested whether the $Ca^{2+}$ current (iCa), a decisive factor to $Ca^{2+}$ entry, was altered in post-thawed oocytes by using whole cell voltage clamp technique. The quality and survival rates of the oocytes derived from both fresh and frozen groups were examined by morphology and FDA-test. Vitrified oocytes (VOs) were incubated for 4 hr after thawing and then donated to this experiment. Ethyleneglycol-ficoll-galactose (EFG) was used as a cryoprotectant for vitrification. The membrane potential was held at -80 mV and step depolarizations of 250 ms were applied from -50 mV to 50 mV in 10 mV increments. The survival rates showed a higher in VOs vitrified with EFG containing $Ca^{2+}$ than in VOs vitrified with EFG under the $Ca^{2+}$-free condition (82.0% vs 14%). In group with/without $Ca^{2+}$, the survival rates were significantly (P<0.01) difference. In the fresh metaphase II oocytes (FOs), current-voltage (I-V) relationship showed that iCa began to activate at -40 mV and reached its maximum at -10 mV. With same voltage pulses, inward currents were elicited in VOs. I-V relationships observed in VOs were similar to those in FOs. Time constants of activation and inactivation of the inward current shown in VOs were not different to those in FOs. This accordance in I-V relations and time constants in FOs with those in VOs indicates that the inward currents in FOs are unaltered by vitrification and thawing. Therefore, vitrification with EFG does not play as a factor to deteriorate $Ca^{2+}$ entry across the membrane of the oocytes.

Occurrence of Freesia Basal Rot Caused by Sclerotium sp. (Sclerotium sp.에 의한 프리지아 균핵병 발생)

  • Lee Sang-Yeob;Ryu Jae-Gee;Kim Yong-Ki
    • Research in Plant Disease
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    • v.12 no.2
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    • pp.69-74
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    • 2006
  • Basal rot of freesia caused by a Sclerotium sp. occurred at Incheon areas. Incidence of the disease reached up to 45% and averaged 17.0% in the fields. Typical symptoms consisted of sheath dry and leaf blight due to rots on basal leaves. The causal fungus was identified as Sclerotium sp. based on following mycological characteristics. The fungus formed sclerotia on cultural media and plant tissues, but did not produce asexual spores. On cultural medium, aerial mycelia of the fungus changed color from white to clay with cultural age and smelled musty odor. Numerous irregular and elliptical black microsclerotia of the fungus were formed on potato dextrose agar (PDA) after 5 days of incubation at $25^{\circ}C$ and sized $115{\sim}200{\times}95{\sim}150 (av. 145{\sim}126.5){\mu}m$. The fungus grew at $10{\sim}32^{\circ}C$ and $pH 4.0{\sim}8.5$. However, the optimal temperature and pH for mycelial growth of the fungus were $24^{\circ}C$ and 5.5 respectively. The isolate showed present pathogenicity to not only freesia but gladiolus in the pathogenicity test, and the symptoms were similar to those observed in the fields. Basal rot of freesia caused by Sclerotium sp. is firstly reported in Korea.

A Mid-Maturing Apple Cultivar "Hongso", High Density Cultivation Type having a Good Taste (밀식재배형 식미우수 중생종 사과 "홍소(紅笑)")

  • Kim, Mok Jong;Kwon, Soon Il;Paek, Pong Nyeol;Nam, Jong Chul;Kang, Sang Jo;Shin, Yong Uk;Hwang, Jung Hwan;Kang, In Kyu;Choi, Cheol
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.556-559
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    • 2009
  • "Hongso" was originated from a cross between "Yoko" and "Hongro" in NIHHS, RDA, in 1989. "Hongso" was preliminarily selected in 2002 for its high fruit quality. After regional adaptability test at five sites from 2003 to 2006 as "Wonkyo Ga-30" it was finally selected in 2006. Optimum harvest time is early September which is similar to that of "Hongro". Fruit shape is globose conical and skin color is light red. Mean fruit weight is 295g and soluble solids content is 14.1oBrix. Fruits acidity is 0.34%, which is higher than that of "Hongro"(0.23%). It has a good taste for harmony of sugar and acidity. Storability is 3 weeks in room temperature. It is apt to russet on fruit skin. It is susceptible to Bitter rot. "Hongso" has a good cross compatibility with major cultivars such as "Fuji", "Hongro" and "Tsugaru". Tree habit is semi-spreading and tree vigor is weak. "Hongso" is high-density cultivation type cultivar, because it has a precocity, spur-type.

Effect of Organic Fertilizer Application on the Growth and Yield of Leaf Lettuce and Garland Crysanthemum (유기질비료(有機質肥料)(Bio-com)시용(施用)이 상치와 쑥갓의 생육(生育) 및 수량(收量)에 미치는 영향)

  • Kim, Jeong-Je;Cho, Byoung-Ok;Lee, Sang-Kyu
    • Korean Journal of Soil Science and Fertilizer
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    • v.20 no.2
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    • pp.169-177
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    • 1987
  • Effect of microbiological product manure (Bio-com) on the growth and yield of vegetables and on physico-chemical properties of soil. Leaf lettuce and Garland Chrysanthemum were taken for test plants. A Jungdong (Series) silty loam soil was used for pot experiments. The results are summarized as following: 1. Treatment with Bio-com stimulated plant growth that were increased in the number of leaf, leaf area, plant height and root length both for Leaf lettuce and Garland Cyrysanthemum. 2. Yield increase of 123-1170% was obtained from Bio-com treatments compared to N.P.K.+manure treatment. Application of 2250 kg/10a of Bio-com gave the highest yield. 3. Fertilizer effect of Bio-com appeared much earlier than that of manure. 4. Plant content of $K_2O$ was highest when treated with Bio-com. Plants absorbed considerably more N, $P_2O_5$ and MgO. 5. Content of N, $P_2O_5$ in Garland Chrysanthemum were higher when treated with Bio-com. 6. Content of $P_2O_5$ in soil was at the highest level. Soil pH, content of exchangeable soil Ca and Mg were also high with Bio-com fertilization.

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