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Isolation and Characterization of Plant Growth Promoting Rhizobacterium Bacillus subtilis YK-5 from Soil  

Yeo, Soo-Hwan (Department of Korean Food Research for Globalization, NAAS, RDA)
Yook, Young-Min (Keimyung Biotech Co.)
Kim, Hyun-Soo (Department of Microbiology, College of Natural Science, Keimyung University)
Publication Information
KSBB Journal / v.24, no.4, 2009 , pp. 334-340 More about this Journal
Abstract
For the isolation of a plant growth-promoting rhizobacteria, strain YK-5 was selected from approximately 400 thermostable strains isolated from special soil samples. Strain YK-5 produced an antifungal compound, and optimum carbon and nitrogen sources for the production of the antifungal compound were investigated against Aspergillus flavus as a test strain. Modified LB medium containing 1% peptone, 1% yeast extract and 5% black sugar was determined to be the optimal medium for growth and antifungal compound production. Culture broth of strain YK-5 potently inhibited growth of the phytopathogenic fungus Fusarium oxysporum KACC 40052 for 7 days. The plant growth-promotion function of strain YK-5 was tested against radish and rice in pot trials. Leaf number, plant height and root length in YK-5-treated radish markedly exceeded (> 60%) those of untreated radish. Leaf length and white rootlet development were markedly more prominent than in commercially-treated rice plants. Strain YK-5 was determined to be Bacillus subtilis YK-5 by physiological, chemotaxonomical, and phylogenetical analyses.
Keywords
Plant growth-promoting rhizobacteria; antifungal activity; Bacillus sutilis YK-2; phytopathogenic fungi;
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Times Cited By KSCI : 2  (Citation Analysis)
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