• Title/Summary/Keyword: RDA test

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Bio-mechanical Analysis of the Grapevine Cluster Thinning Task using Working Chair

  • Lee, Kyung-Suk;Kim, Hyo-Cher;Chae, Hye-Seon;Kim, Kyung-Ran;Lim, Dae-Seop
    • Journal of the Ergonomics Society of Korea
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    • v.30 no.3
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    • pp.395-401
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    • 2011
  • Objective: This study examined the effects of working chair that was developed for farmers who work in grapevine's cluster thinning. Background: Agricultural work involves some of the nation's highest occupational risk exposures. Fruit cultivation has been recognized as one of the most hazardous crops in which to work. Grapevine cluster thinning task involve activities related to the main risk factors associated with upper limb work-related musculoskeletal disorders. Method: An experiment was designed to test the working chair. Six healthy male($25.8{\pm}4.9years$) were selected as the subjects for this study. Electromyography(EMG) was used to monitor the muscles activity, and Electro-goniometer was used to measure the ranges of motions of the elbow, trunk and knee. Subjective test was also carried out to examine discomfort body parts and their pain intensity. A grapevine's working space was built for the experiment and working chair was installed on it. In order to examine the muscle activity and range of motion, subjects used to the working chair for 30 minutes for each experimental condition. Another test without working chair was also performed for comparison. %MVC was used to quantify the level of muscle activity. Results: Decreases of muscle activity was found in all leg muscles and significant decrease of muscle activity was found in left Gastrocnemius. The range of motion of the trunk and knee also decreased when working chair used. Discomfort in lower back, thigh and shank region were reduced significantly. However, in upper limbs muscle activity tended to increase in working chair compared with conventional task. Conclusion: Improvement for cushion in seat back and pan required to reduce discomfort in buttocks. Application: Overall findings verified that the working chair might help to prevent upper limb and lower back MSDs based on the current study. These results can be practically used for work improvement for the grapevine growers to prevent MSDs.

Mutagenicity Studies of the Herbicide-resistance Phosphinotricin Acetyltransferase (PAT) (제초제저항성단백질 Phosphinotricin Acetyltransferase (PAT)의 유전독성시험)

  • Jeong, Mi-Hye;You, Are-Sun;Lee, Je-Bong;Shin, Jin-Sup;Kim, Jin-Hwa;Han, Jeung-Sul
    • The Korean Journal of Pesticide Science
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    • v.8 no.1
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    • pp.22-29
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    • 2004
  • To evaluate mutagenicity of Phosphinotricin Acetyltransferase(PAT) which is expressed by the glufosinate-resistance gene pat, in vitro reverse mutation test using Salmonella typhimurium, chromosome aberration test using chinese hamster lung(CHL) cells and in vivo micronucleus test of mice were performed. In the reverse mutation, the PAT did not induce mutagenicity in Salmonella typhimurium TA 98, TA 100, TA 1535, TA 1537 with and without metabolic activation at $5000{\mu}g/plate$. In the chromosome aberration test, the results showed no incidence of increased structural and numerical chromosome aberrations at any doses tested(100, 10, $1{\mu}g/mL$). In micronucleus test, the ratio of micronuclei was measured in polychromatic erythrocytes of bone marrow of male ICR mice intraperitoneally administrated with PAT(1250, 625, and 313 mg/kg), the results showed no incidence of increased micronucleated polychromatic erythrocytes (MNPCE). These results indicate that PAT might not have mutagenic potential in vitro and vivo systems.

Development of test method for the evaluation of pesticide acute toxicity using earthworm(Lumbricus rubellus) (붉은지렁이 (Lumbricus rubellus)를 이용한 농약의 급성독성 시험법 개발)

  • Park, Yeon-Ki;Park, Kyeong-Hoon;Kim, Byung-Seok;Kyung, Kee-Sung;Shin, Jin-Sup;Oh, Byung-Youl
    • The Korean Journal of Pesticide Science
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    • v.4 no.4
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    • pp.56-60
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    • 2000
  • A study was performed to determine the maintenance conditions of the earthworm, Lumbricus rubellus, for the acute toxicity test. To fine out climatic and soil conditions, the earthworms were maintained in artificial soil consisting of sand, clay mineral and peat at different levels of conditions for 14 days. Lumbricus rubellus led to an increase of biomass at temperature $22{\pm}2^{\circ}C$, soil pH $7.0{\pm}1$ and moisture 40%. And four fungicides were tested for acute toxicities to Lumbricus rubellus, according to the optimum condition. The test earthworms were exposed to each pesticide with various concentration gradients. After 14 days, the number of surviving earthworms and their weight alteration during the test period was determined. The 14-day $LC_{50}$ values for the Lumbricus rubellus, of carbendazim, benomyl, thiophanate-methyl and thiabendazole were determined to be 59, 53, 64 and 36 mg/ kg soil dry weight, respectively.

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A Longitudinal Study on the Change of Nutrients and Food Consumption with Advance in Age among Middle-aged and the Elderly (중.노년층에서 연령증가에 따른 영양소 및 식품섭취의 양적, 질적 변화에 대한 종단적 연구)

  • 김인숙;서은아;유현희
    • Korean Journal of Community Nutrition
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    • v.4 no.3
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    • pp.394-402
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    • 1999
  • The purpose of this study is to investigate diet quality and changes in nutrient and food intake with increase in age. Subjects were 69 patients(28 males, 41 females) living in Jeon-ju city, over middle-aged, and they were the same patients studied 4-7 years ago in a previous study. Dietary survey with one day 24-hour recall method was used. Energy, calcium, vitamin A and vitamin B$_2$intakes were lower than the RDA and vitamin C was in excessive status in pre-test and post-test. In food groups examrned, the consumption of potatoes, beans, vegetables, seaweeds, beverages, seasonings, oils, fish & shells and milks was increased amount than 4-7 years before but the consumption of cereals(p<0.01), sugars, seeds, fruits, processed food, meats and eggs was decreased. Diet quality was assessed by %RDA, nutrient adequacy ratio(NAR), mean adequacy ratio(MAR), dietary diversity score(DDS), meal balance and food group pattern. In %RDA, those proportion of appropriate intake in post-test in were higher than those in pre-test. For most nutrient except vitamin A, the levels of NAR in post-test were higher than in pretest. The MAR, an index of overall dietary quality, was 0.77 for pre-test and 0.83 for post-test(p<0.05). When we counted the major food groups consumed(KDDS), 62.3% of subjects had a KDDS of 3 in pre-test and 42.0% of subjects had a KDDS of 4 in post-test. Based on these results, in terms of variety and balance, total diet quality was improved with advancing of age.

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A New Rice Variety with Low Amylose, 'Manmibyeo' (중만생 저아밀로스 반찹쌀 품종 '만미(萬味)벼')

  • Song, You-Chun;Lee, Jong-Hee;Lee, Jeom-Sig;Ha, Woon-Goo;Park, No-Bong;Kwak, Do-Yeon;Jung, Kuk-Hyun;Oh, Byeong-Geun;Yeo, Un-Sang;Kang, Jong-Rae;Yi, Gi-Hwan;Jang, Jae-Ki;Nam, Min-Hee;Lim, Sang-Jong;Kwon, Oh-Kyung;Hwang, Hung-Goo
    • Korean Journal of Breeding Science
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    • v.42 no.3
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    • pp.257-261
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    • 2010
  • 'Manmibyeo', a new japonica rice cultivar, is a mid-late maturing ecotype cultivar developed by the rice breeding team of Department of Functional Crop, NICS, RDA in 2002. This cultivar was originated from the cross between 'Milyang 95' and $F_1$ of the cross between 'Bukrukbanna' and 'Milyang 95' in 1990/1991 winter season. It was selected by pedigree breeding method until F6 generation, and a promising line, YR14545-9-2-3-4, was advanced and designated as 'Milyang 162' in 1996. The local adaptability test of 'Milyang 162' was carried out at seven locations during 3 years in 1997, 1998 and 2002. It has about 86cm culm length and is lodging tolerant. This cultivar is susceptible to bacterial blight ($K_1$, $K_2$, $K_3$) and stripe virus, and moderately resistant to leaf blast disease. Milled rice kernels of 'Manmibyeo' is translucent with low amylose content in endosperm, clear in chalkiness and good at eating quality in panel test. The yield potential of 'Manmibyeo' in milled rice was about 4.46 MT/ha at ordinary fertilizer level in local adaptability test. This cultivar would be adaptable to the southern plain of Korea.

Effects of Achyrantes japonica on Carrageenan-Induced Arthritis Rat Model (Carrageenan으로 유발한 관절염 쥐에서의 우슬추출물 효과)

  • Kim, Young-Ock;Lee, San-Won;Lee, Seung-Eun
    • Korean Journal of Medicinal Crop Science
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    • v.17 no.6
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    • pp.470-474
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    • 2009
  • Achyrantes japonica (AJ) has been used to treat edema and arthritis in the traditional Korean medicine. To elucidate the anti-inflammatory and anti-nociceptive effects of ethanol extract of AJ, the carrageenan-induced paw edema using a plethysmometer and thermal hypersensitivity using the plantar test were measured. Ibuprofen was used as a control drug. Treatment with AJ (200mg/kg p.o.) significantly reduced paw edema, compared to the carrageenan - treated rats. In the plantar test, the thermal withdrawal latency in AJ - treated group was significantly increased than the carrageenan - treated group. The results indicate that AJ could have be the anti-inflammatory and anti-nociceptive properties.

'Saemimyeon', a Tongil-Type Medium-Late Maturing Rice Variety with High Amylose ContentUsed for Rice Noodle Preparation (쌀면전용 고아밀로스 중생 통일형 벼 '새미면')

  • Cho, Jun-Hyeon;Lee, Jong-Hee;Park, No-Bong;Son, Young-Bo;Oh, Sung-Hwan;Han, Sang-Ik;Song, You-Chun;Seo, Woo-Duck;Park, Dong-Soo;Nam, Min-Hee;Lee, Ji-Yoon
    • Korean Journal of Breeding Science
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    • v.50 no.4
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    • pp.522-528
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    • 2018
  • Saemimyeon, a Tongil type, medium-late maturing rice variety, is especially used for preparing rice noodles. Its high amylose content was developed to fit market demands and to be affordable for rice processing industries. One of the high yielding lines, Milyang181 (Hanareum), was used in the final three-way cross of $IR50^*2$/YR18241-B-B-115-1-1 for yield improvement and cultivation stabilization, including disease resistance. YR24235-10-1-3, a high yielding and compact plant type, was selected and named Milyang278 after yield test at NICS (RDA, Miryang) in 2010. It was subjected to regional yield test at six sites in the middle and southern plain areas of South Korea. Saemimyeon heading occurs on August 12 and is a mid-late maturing cultivar, with resistance to leaf blast, rice stripe virus, and bacterial blight (K1-K3a), but it is susceptible to major diseases and insect pest infestation. Saemimyeon showed a high amylose content of 26.7%, with a relatively low KOH digestion value of 3.5, which are key factors in rice noodles and pasta processing. In the local adaptability tests, the yield of Saemimyeon was 7.08 MT/ha-an increase of approximately 106% compared to that of Dasan. Thus, Saemimyeon is suitable for cultivation in the southern and middle plain areas of South Korea.

Development of Rapid Diagnostic Technology for Pig Disease (2) - Rapid detection of PPE in the pig feces -

  • Kim, Hyuck-Joo;Hong, Jong-Tae;Yu, Byeong-Kee;Kim, Giyoung;Kim, Suk
    • Journal of Biosystems Engineering
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    • v.38 no.2
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    • pp.121-128
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    • 2013
  • Purpose: Porcine proliferative enteropathy (PPE), caused by the obligate intracellular bacterium Lawsonia intracellularis, is a widely distributed disease throughout the world causing substantial economic loss. In order to diagnose PPE rapidly, the rapid kit was developed and tested. Methods: In this study, a rapid kit was developed to screen the PPE rapidly at the pig farm. Also, occult blood test with fecal occult blood (FOB) kit was done for detecting the blood in pig feces which might be the evident of hemorrhagic PPE. For developing the kit, we tested fecal samples of PPE infected pigs diagnosed by polymerase chain reaction (PCR) method. Results: With the developed rapid kit, Lawsonia intracellularis was detected in high density emulsion of ileum. On the other hand, the test result of detecting Lawsonia in feces showed too high non-specific response. In addition, nevertheless the FOB test result showed that blood evident could be founded in pig feces, the diagnosing result was not fit to PCR test result, which shows blood in pig feces could be from not only hemorrhagic PPE but also many reasons. Conclusions: To deal with the PPE effectively, it will be better for farmers to screen the PPE in earlier stage with easy and rapid diagnosing tool on farm. This study found out that the rapid kit could detect the Lawsonia intracellularis and hemoglobin in pig feces. However, the non-specific response to negative samples of PPE was too high to use at a pig farm. Further research is needed for lowering the non-specific response with the rapid kit.

Development of an electronic sow liquid feeding system using RFID (1) - Development and performance test of the prototype - (RFID이용 전자식 모돈 개체별 액상급이기 개발(1) - RFID를 이용한 모돈 액상급이기 제작 및 성능시험 -)

  • Kim, Hyuck Joo;Hong, Jong Tae;Yu, Byeong Kee;Kim, Sang Cheol;Choi, Kyu Hong;Chang, Hong Hee
    • Journal of Biosystems Engineering
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    • v.37 no.6
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    • pp.373-384
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    • 2012
  • Purpose: In EU, the animal welfare regulations are strengthening. Also, in Korea, for strengthening of animal protection laws, animal welfare farm certification scheme is being prepared to enforce. To get the certification of animal welfare farm, especially for sows, individual specific feeding in sow group is necessary in accordance with the animal welfare regulation. Also, liquid feeding is reported to be more effective for farrowing as well as dry sow. Therefore, in this study, a electronic liquid sow feeder using RFID which could be fed to farrowing sow simultaneously is developed. Methods : We manufactured a prototype of appropriate elements which were evaluated in the preliminary test for selecting RFID tag, supply pump, mixing type, etc. With the prototype, the performance test for liquid feeding gilt was done in group raising training barn. Results : The performance test result shows the C.V.(coefficient of variation) of liquid mixing and feeding is 0.77~1.97% and 2.4~5.3%, respectively, which means the system could feed sows uniformly. The feeding time of pneumatically activated ball valve is 2 seconds per 0.9 kg of liquid feed. The eating time for gilt were 65 seconds in average. Conclusions : The prototype could feed sow uniformly, and deliver the liquid feed 9 times for a sow continuously. Also, total eating time for a sow was 18 minutes in one visit to the prototype of feeding station.

Genetic Analysis on Germination Rate and Fat Acidity of Japonica Rice Seeds after High Temperature Storage

  • Noh, Gwang-Il;Kim, Ki-Young;Shin, Seo-Ho;Park, Hyun-Su;Kim, Bo-Kyeong;Shin, Mun-Sik;Ko, Jae-Kwon
    • Korean Journal of Breeding Science
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    • v.40 no.3
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    • pp.228-233
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    • 2008
  • This study was carried out to get the genetic information on the germination rate and fat acidity after 12 weeks' storage at $35^{\circ}C$. Germination rate decreased with longer storage period, while fat acidity increased. Germination rate was higher in the order of Koshihikari, Hanmaeum and Unkwangbyeo after 12 weeks' storage at $35^{\circ}C$. Fat acidity of Koshihikari and Hanmaeum was gradually increased, but that of Unkwangbyeo was dramatically increased after 4 weeks' storage at $35^{\circ}C$. The genetic mode on germination rate and fat acidity using the joint scaling test was shown to be additive-dominance gene effects. Additive([d]) effect was higher than dominance effect([h]) in all crosses. The average degree of dominance, [h]/[d], was less than 1, indicating that germination rate and fat acidity was partially dominant. Germination rate and fat acidity based on frequency distribution of 2 crosses were quantitative characters expressed by polygenes.