• Archives of Pharmacal Research
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• 제21권4호
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• pp.378-384
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• 1998

The processing pathway of G-proteins and Ras family proteins includes the isoprenylation of the cysteine residue, followed by proteolysis of three terminal residues and .alpha.-carboxyl methyl esterification of the cysteine residue. Farnesylcysteine methyltransferase (FCMT) activity is responsible for the methylation reaction which play a role in the membrane attachment of a variety of cellular proteins. Four kinds of Ras protein (c-Ha-ras, c-N-Ras, c-Ki-Ras, pan-Ras) expression were detected in adenocarcinoma of human tissue by immunohistochemical method, and hematoxylin and eosin staining. The level of Ras protein in human stomach tumor tissues was much higher than in normal and peritumoral regions of the same biopsy samples. The FCMT activities of each cellular fractions were high in mitochondrial fraction followed by microsomal fraction, whole homogenate and cytosolic fraction. The inhibitory effect on FCMT activity on stomach tumor tissue was determined after treatment with 0.25 $\mu\textrm{M}$ of S-adenosyl-$_L$-homocysteine. S-adenosyl-$_L$-homocysteine inhibited FCMT activity from 11.2% to 30.5%. These results suggested that FCMT might be involved in Ras proteins activity.

• 대한화학회지
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• 제66권5호
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• pp.349-361
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• 2022

Because hydrotreating (HDT) of FCC gasoline is one of the important processes used to prepare such gasoline for blending, the development of a catalyst for this process is of great interest. Currently, the industrial HDT of FCC gasoline consists of two stages and the creation of a new catalyst for one-stage HDT will make this process more efficient. Recently, our group has developed the CoMo/Al₂O₃-ASA catalyst and studied the influence of Si/Al ratio on the target reactions of HDT process. Despite the high selectivity and activity, the catalyst with ASA is not applicable in industry because of its low strength. The present work moves forward to study the influence of the ASA content in the catalyst support and clarify the possibility to develop the catalyst that combines high activity and selectivity in HDT reactions with successful performance. Here we show that the CoMo catalyst with ASA/Al₂O₃ molar ratio 1/1 in the support is the best combination for FCC gasoline hydrotreatment due to exceptional properties of the catalyst composition.

• Molecules and Cells
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• 제43권10호
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• pp.889-897
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• 2020

K-RAS is frequently mutated in human lung adenocarcinomas (ADCs), and the p53 pathway plays a central role in cellular defense against oncogenic K-RAS mutation. However, in mouse lung cancer models, oncogenic K-Ras mutation alone can induce ADCs without p53 mutation, and loss of p53 does not have a significant impact on early K-Ras-induced lung tumorigenesis. These results raise the question of how K-Ras-activated cells evade oncogene surveillance mechanisms and develop into lung ADCs. RUNX3 plays a key role at the restriction (R)-point, which governs multiple tumor suppressor pathways including the p14^ARF-p53 pathway. In this study, we found that K-Ras activation in a very limited number of cells, alone or in combination with p53 inactivation, failed to induce any pathologic lesions for up to 1 year. By contrast, when Runx3 was inactivated and K-Ras was activated by the same targeting method, lung ADCs and other tumors were rapidly induced. In a urethane-induced mouse lung tumor model that recapitulates the features of K-RAS-driven human lung tumors, Runx3 was inactivated in both adenomas (ADs) and ADCs, whereas K-Ras was activated only in ADCs. Together, these results demonstrate that the R-point-associated oncogene surveillance mechanism is abrogated by Runx3 inactivation in AD cells and these cells cannot defend against K-Ras activation, resulting in the transition from AD to ADC. Therefore, K-Ras-activated lung epithelial cells do not evade oncogene surveillance mechanisms; instead, they are selected if they occur in AD cells in which Runx3 has been inactivated.

• 한국지반환경공학회 논문집
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• 제17권9호
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• pp.21-28
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• 2016

본 연구의 목적은 유역규모별 홍수침수면적을 산정하기 위한 하천지형 구축기법과 그에 따른 침수면적을 비교하는 것이다. 대상 지역은 경상북도 청도군과 의성군 내 14개소 하천이며 수치지도 DEM, ASTER DEM과 SRTM DEM을 이용하여 하천지형을 구축하였으며, HEC-GeoRAS, RAS Mapper, RiverCAD 모형을 적용하여 홍수침수해석을 수행하였다. 해석 결과와 실제 침수흔적도와 비교하였으며, 각 해석 모형들과 하천지형 구축기법에 따라 침수면적을 비교하였다. 침수흔적도와 비교한 결과 HEC-GeoRAS 모형의 결과가 이 침수흔적도의 범람면적과 대부분의 유역에서 유사한 값을 나타내었으며, SRTM DEM이 ASTER DEM보다 수치지도 DEM과 유사한 결과를 나타내었다. 중 대규모 유역의 경우 HEC-GeoRAS 모형과 Ras Mapper 모형, 소규모 유역의 경우 HEC-GeoRAS 모형과 RiverCAD 모형이 효율적인 해석 모형으로 나타났다.

• 한국산학기술학회논문지
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• 제17권4호
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• pp.726-733
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• 2016

최근 집중호우로 인한 홍수발생 빈도와 그 규모가 커지고 있다. 이러한 현상을 반영하여 많은 국가에서는 향후 발생가능한 홍수로 발생할 수 있는 피해에 대한 위험성에 대한 인식을 국민에게 제고할 수 있는 홍수위험지도가 제작되고 있다. 홍수위험지도는 다양한 홍수범람해석모형을 통해 작성되는 데 침수원인에 따라 외수범람해석모형과 내수범람해석모형으로 구분할 수 있다. 국내 외적으로 다양한 홍수범람해석모형이 사용되고 있지만 미육군공병단에서 개발된 HEC-RAS 모형을 제외한 대부분은 고가의 상용 프로그램으로써 그 사용성이 제한되어 있는 것이 사실이다. 이러한 상황에서 현재 미육군공병단에서는 기존 1차원 모형인 HEC-RAS 모형과 연계 가능한 2차원 모형을 개발 중에 있다. 이 모형은 HEC-RAS 5.0이라는 명칭으로 베타버전이 공개되었다. 본 연구에서는 곡교천을 대상으로 선공개된 HEC-RAS 5.0 모형 베타버전과 국내 홍수위험지도 작성 시 사용되는 FLUMEN 모형과의 비교를 통해 모형의 특징, 사용성, 적용성, 결과의 정확성 등을 평가하였다. 본 연구를 통해 얻은 결과는 향후 HEC-RAS 5.0 모형이 안정되어 서비스 되면 외수침수범람해석과 관련된 사업과 연구 등 다방면에 활용될 수 있을 것으로 판단된다.

• 인간행동과 음악연구
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• 제12권2호
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• pp.19-36
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• 2015

본 연구는 외상성 뇌손상 청소년을 대상으로 리듬청각자극(RAS) 적용 시 선율의 유무에 따른 보행 훈련 효과를 비교하고, 보행인자의 변화를 알아보고자 시행하였다. 이를 위해 3명의 외상성 뇌손상 청소년을 대상으로 사전 사후 평가를 제외한 총 8회의 보행훈련을 진행하였다. 회기별 중재 시 선율 유무에 따른 보행 속도 차이를 비교하였고, 훈련 효과에 따른 시공간적 지표 및 운동 형상학적 지표 변화 비교를 위해 VICON 370 Motion Analysis System을 사용하여 보행 지표를 측정하였다. 연구 결과 RAS 보행 훈련 시 제공한 음악 자극 형태에 따라 보행 속도에 미치는 유의미한 영향이 없으므로, 선율의 사용 유무는 보행 훈련 효과와 직접적인 관련이 없음을 알 수 있었다. 또한 사전사후 검사 결과, RAS 보행 훈련 후 분속수, 보행 속도, 활보장, 활보장 시간, 보행 대칭성이 공통적으로 개선되었으며, 대상자 모두 공통적으로 고관절 및 슬관절의 움직임에서 필요 이상의 과도한 굴곡이나 신전이 감소함에 따라 양측의 비대칭성 감소 및 움직임의 안정성이 증가한 모습을 보였다. 리듬 적용과 리듬 자극에 선율을 반영한 음악 자극 적용에 따른 보행 속도 간 차이를 비교한 본 연구 결과는 운동기능을 위한 RAS 적용 시, 일정한 간격의 리듬자극에 의한 리듬-운동 동조화가 핵심 기제로 작용함을 시사한다. 또한, 본 연구는 외상성 뇌손상 청소년을 대상으로 RAS 보행 훈련을 통한 보행 기능뿐만 아니라 보행 패턴의 개선을 살펴본 것에 의의가 있다.

• 치과방사선
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• 제28권1호
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• pp.245-259
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• 1998

Cellular transforming genes have been identified in a number of different tumor cell lines and tumor types. A significant number of these oncogenes belong to the ras gene family. The ras gene family consists of three closely related genes:H-ras, K-ras and N-ras which code for a related 21 kDa protein. Mutations in codon 12, 13 and 61 of one of the three ras genes convert these genes into acute oncogenes. The presence of H-ras gene mutations has important prognostic implications in various tumors. Each genomic DNA was isolated from tumors induced by implantation with DMBA, or by treatment with DMBA -implantation/irradiation. When genome DNA was transfected into NIH 3T3 cells and investigated by two-step PCR-RFLP, the fOllowing results were concluded: 1. Transformation foci developed in two groups when the genome DNA of two experimental groups were transfected into NIH 3T3 cells. 2. Transformation efficiency was 0.01-0.02 foci/㎍DNA in the experimental group with the DMBA-implantation, 0.01-0.03 foci/㎍lgDNA in the experimental group with the DMBA-implantation/irradiation according to results of transfection assay. 3. When the point mutation of H-ras gene was investigated by a two-step PCR-RFLP, there was 13.9％ (5/36) in the experimental group with the DMBA implantation, 15.4 ％ (6/39) in the experimental group with the DMBA -implantation/irradiation. 4. The point mutation in codon 12 and 61 of H-ras was 5.6％(2/36) and 8.3％(3/36) in the experimental group with the DMBA implantation. 5. The point mutation in codon 12 and 61 of H-ras gene was 7.7％(3/39) in the experimental group with the DMBA -implantation/irradiation.

• Asian-Australasian Journal of Animal Sciences
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• 제7권1호
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• pp.119-124
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• 1994

Experiments were performed to determine age-associated changes in ornithine decarboxylase (ODC) gene and Ha-ras cellular oncogene expression in tissues of female rats. In the kidney, ODC mRNA levels did not show age-associated changes, while ODC enzyme activities were decreased with advancing age from 3 to 10 months. These results suggest that post-transcriptional mechanism (s) are involved in the age-dependent decrease in renal ODC enzyme activity. In addition, we found no correlation between testosterone-induced renal ODC expression and DNA methylation pattern. Ha-ras mRNA levels in brain decreased as animals aged from 3 to 6 months, while renal Ha-ras mRNA levels were not influenced by age. Results demonstrate the age-dependent expression of Ha-ras in a tissue-specific manner.

• 한국농공학회:학술대회논문집
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• 한국농공학회 2001년도 학술발표회 발표논문집
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• pp.314-318
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• 2001

The analysis of the spatial extent of flood inundation is important for flood mitigation. Geographic Information System (GIS) has advantage of analyzing spatial distributed data. Hydrologic Engineering Center's River Analsysis System(HEC-RAS) with HEC-GeoRAS was used to analyze flood inundation. HEC-GeoRAS, which is an ArcView GIS extension designed to process geospatial data for HEC-RAS, is a useful tool for storing, managing, analyzing, and displaying spatially distributed data. Rational formula and 24-hr duration probability precipitation data of Suwon meteorological station were used to estimate the flood runoff. And water profiles were calculated using the HEC-RAS model with HEC-GeoRAS. The flooded region is 8.24ha when 50-yr probability precipitation was applied and 8.8ha when 100-yr was applied to Bahlan study watershed which is located in Whasung county, Kyunggi province, having an area of $29.79km^{2}$.

• Asian Pacific Journal of Cancer Prevention
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• 제14권5호
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• pp.2699-2701
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• 2013

The RAS family genes encode small GTP-binding cytoplasmic proteins. Activated KRAS engages multiple effector pathways, notably the RAF-mitogen-activated protein kinase, phosphoinositide-3-kinase (PI3K) and RalGDS pathways. In the clinical field, K-ras oncogene activation is frequently found in human cancers and thus may serve as a potential diagnostic marker for cancer cells in circulation. This mini-review aims to summarise information on Ras-induced oncogenesis and the clinical significance of K-ras.