• Korean Journal of Environment and Ecology
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• v.11 no.4
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• pp.407-414
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• 1998

The rare and endangered plants in Naesorak area were investigated for four months during May to August in 1997, and the results were summarized as 35 taxa which is classified as 19 families, 32 species, and 3 varieties. At the level of families, 19 families such as Pinaceae(1 species), Taxaceae(1 species), Cupressaceae(1 species and 1 variety), Liliaceae(5 species and 1 variety), Aristolochiaceae(2 species), Ranunculaceae(4 species), Berberidaceae(1 species), Saxifragaceae(2 species), Rosaceae(1 species), Oleaceae(2 species and 1 variety), Solanaceae(1 species), Scrophulariaceae(1 species), Valerianaceae(1 species), Campanulaceae(1species), and Compositae(1 species) were recorded. The most of the habitats of the species was easily found along the major mountain trails, and a kind of destructions of these plants by human interferences is due to the fact that the illegal collections for food and medicine are occurred. Those species of Taxus caespitosa, Rhododendron aureum, Vaccinium bracteatum, Allium victoralis var. platyphullum and Leontice microryncha has a few individuals in habitats and also shows the unfavorable growing state, so the intensive and continuous habitat management of the habitats were inevitably dimanded.

• Journal of Bio-Environment Control
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• v.31 no.2
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• pp.133-141
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• 2022

Bulbophyllum auricomum Lindl. is a rare orchid and has flowers with an attractive fragrance. The present study investigated the tissue culture method for micropropagation. Capsules derived from artificial self-pollination were obtained for the best seed germination in MS basal medium. Plant growth regulators (1.0 mg·L^-1 of BAP and 2.0 mg·L^-1 of NAA) were affected by callus induction from subcultured pseudobulb explants. For the callus subculture, different natural plant extracts were tested in 11 treatment media. Among them, MS medium with 150 mL·L^-1 of coconut water was generally effective in fresh weight (1.75 ± 0.08) and (3.01 ± 0.20) of callus proliferation and PLBs induction at 1 and 2 months, respectively, followed by an MS combination of 30 g·L^-1 of banana and 20 g·L^-1 of potato extract. The results of a comparative study of different MS mediums containing plant growth regulators with a natural extract combination and MS medium supplemented with natural extract only showed that MS medium supplemented with a combination of natural extracts (150 mL·L^-1 of coconut water) and plant growth regulators (2.0 mg·L^-1 of BAP and 1.0 mg·L^-1 of NAA) obtained the highest shoot regeneration (3.37 ± 0.17) and (6.41 ± 0.68) after 1 month and 2 months of culturing, respectively.

• Korean Journal of Environmental Biology
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• v.39 no.2
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• pp.245-257
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• 2021

Ulleungdo, a volcanic island, has different climate, soil, and topography characteristics from inland of the Korean Peninsula. Its agriculture also exhibits distinctive features. This study focused on floristic compositions and characteristics of crop lands in Ulleungdo. Flora surveys were conducted for 37 areas three times, one in May of 2016, one in July of 2020, and one in August of 2020. Results showed that vascular plants of crop lands in Ulleungdo included 186 taxa of 53 families, 132 genera, 160 species, 14 varieties, five subspecies, and five hybrids. Asteraceae had the most diverse species (33 taxa), followed by Poaceae (25 taxa), Fabaceae (12 taxa), Polygonaceae (11 taxa), and Liliaceae (nine taxa). The biological type of crop lands in Ulleungdo island was determined to be Th-R₅-D₄-e type. Rare plants were found for nine taxa: Athyrium acutipinnulum Kodama ex Nakai, Sedum takasimense Nakai, Rubus takesimensis Nakai, Dystaenia takesimana (Nakai) Kitag., Ligustrum foliosum Nakai, Aster pseudoglehni Y.Lim, Cirsium nipponicum (Maxim.) Makino, Lilium hansonii Leichtlin ex D.D.T.Moore, and Polygonatum robustum (Korsh.) Nakai. In addition, 45 taxa contained naturalized plants of 13 families, 33 genera, 44 species, and one variety. Urbanization and naturalization indices were 13.9%, and 24.2%, respectively.

• Korean Journal of Environment and Ecology
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• v.35 no.4
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• pp.347-376
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• 2021

Investigating and evaluating regionally distributed plant resources provide fundamental information (inventory) to conserve plant diversity in target areas. Nevertheless, the examination of inventory to collect information in areas with little interest has been insufficient. In this study, the vicinity of Mt. Baegamsan(1,004m) was investigated for plant resources in the central and southern part of the Korean peninsula, which has received low interest. Nine surveys identified a total of 638 taxa consisting of 108 families, 339 genera, 572 species, 12 subspecies, 49 varieties, and 5 forms. One taxon was designated to be the endangered wild plant by the Ministry of Environment, and a total of 29 taxa were designated to be endemic species in Korea. Floristic target species identified by the surveys included 1 taxon for grade V, 13 taxa for grade IV, 36 taxa for grade III, 29s taxa for grade II, and 34 taxa for grade I. A total of 38 taxa were identified to be naturalized plants, and 3 of them were designated as invasive alien plants by the Ministry of Environment. There was a difference in plant distribution between western and eastern areas of Mt. Baegamsan. The endangered and valuable plants for conservation were mainly identified from Mt. Baegamsan peak area, the ridge, and the western area. Considering the plant distribution, the Mt. Baegamsan region, located on Nakdong-jungmaek, was estimated to be dispersal routes for plant expansion and retreat.

• Korean Journal of Environment and Ecology
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• v.16 no.1
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• pp.104-123
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• 2002

The flora survey on Chungnyeongsan and Seorisan was conducted 6 times from 1998 to 2000.817 taxa such as 112 families 415 genera 688 species 1 subspecies 116 varieties 12 forma were recorded. Dominant species in investigated areas was Quercus mongozica and some deciduous broad-leaved species, such as Quercus sp., Acer sp., Fraxinus sp., Carpinus sp., etc. were codominant species in these areas. Pinus koraiensis and Larix teptozepis plantations were also found in these areas. The 16 taxa of rare plants protected by Forestry Service were found in these areas and among them 10 taxa were new populations which had not been recorded in these areas. Discovery of Erunthis stellata, Aconitum koreanum, Paeonia japonica, Scopotia japonica and Acunthopanax senticosus populations indicate that these areas could be regarded as very important one in a biogeographical aspect. Endemic plants were listed 35 taxa and among them 22 taxa including Corydalis maculata, Ulmus macrocarpa, Scrophutaria horuiensis, Cirsium setidens, etc. were new one. Naturalized plants were listed 39 taxa and among them 30 taxa were new one. Lower naturalized index(NI) and higher urbanized index(UI) were recorded compared to those of neighbored areas. There were many kinds of threatening factors fur the plant resources in survey areas, so it is necessary to limit the number of visitors and to introduce the natural relaxation system.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.15 no.3
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• pp.29-43
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• 2012

This study evaluated 23 botanic gardens and arboretums under two main categories including professional group and operational group. As for professional group, the main difference between the functions of botanic gardens and arboretums was analyzed. In the case of operational group compared professional group respectively, on categories including importance of function and implementation, seeking to provide fundamental data of botanic gardens and arboretums by enhancing the quality of visitors and examining functional differences. The result of a difference in arboretums' function between professional and operational groups, the study analyzed the importance of botanic gardens and arboretums in terms of detailed categories such as research, education, collection, display, retention, and service for visitors. As a result, professional group showed retention 4.32>education 4.11>research 4.09>service for visitors 4.05, and operational group showed retention 3.70>service 3.52>education 3.41>research 3.37, indicating that both groups had retention as the most selected answer. Looking closely at the functions of the currently implemented detailed categories of botanic gardens and arboretums, such as research, education, collection, display, retention, and service for visitors, the study showed that 91.3% selected rare species and individual multiplication for research function, 78.3% chose education of natural ecosystem for children for education, 73.9% answered space for experiencing nature for display, and 73.9% also replied managon, diof rare species, reproduction, and retention of native plants' species for retention category, and service for visitors showed 95.7% for implementation. The study could not clearly point out the influence between predictors due to absence of an established comprehensive evaluation model. Also, it analyzed and reviewed comparison of functions of professional group and operational group, but in the future, it should conduct study on a systemic and objective classification of botanic gardens and arboretums to clearly examine the difference in views between groups, which also requires, at the same time, study on management policy of botanic gardens and arboretums or suggesting guidelines that suit types of forms, and also study on facilities and program development that can be applied to each class.

• Korean Journal of Veterinary Research
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• v.41 no.2
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• pp.123-132
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• 2001

The regional distributions and relative frequencies of some gastrointestinal endocrine cells in the 8 portions (fundus, pylorus, duodenum, jejunum, ileum, cecum, colon and rectum) of the gastrointestinal tract of ICR mouse (ICR) with immunohistochemical method using 7 types of specific antisera against somatostatin, serotonin, glucagon, cholecystokinin (CCK)-8, secretin, pancreatic polypeptide (PP) and gastrin. In this study, somatostatin-, serotonin-, glucagon-, CCK-8-, secretin- and gastrin-immunoreactive (IR) cells were identified. Most of these IR cells in the intestinal portion were generally spherical or spindle in shape (open-typed cell) while cells showing round in shape (close-typed cell) were found in the stomach regions occasionally. Their relative frequencies were varied according to each portion of gastrointestinal tract. Somatostatin-IR cells were demonstrated throughout whole gastrointestinal tract except for large intestine. Serotonin-IR cells were detected throughout whole gastrointestinal tract and they were most predominant endocrine cell types in this species of mouse. Glucagon-IR cells were restricted to the fundus and rectum with moderate and a few frequencies, respectively. CCK-8-IR cells were observed in the pylorus, duodenum and ileum with numerous, moderate and rare frequencies, respectively. Secretin-IR cells were restricted to the duodenum and ileum with a few and rare frequencies, respectively. Gastrin-IR cells were restricted to the pylorus with numerous frequency. However, no PP-IR cells were found in this study. In conclusion, some peculiar distributional patterns of gastrointestinal endocrine cells were found in the ICR mouse compared to those of other mammals.

• Mycobiology
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• v.46 no.2
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• pp.101-113
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• 2018

Lichen genus Stereocaulon (Schreb.) Hoffm is distributed throughout the world. Although 15 Stereocaulon species have been recorded in Korea, no detailed taxonomic or revisionary research has been conducted for nearly two decades. In this study, we collected 260 putative Stereocaulon spp. samples and identified the species based on morphological, chemical, and molecular characteristics. From the collected samples, 10 species of Stereocaulon were identified, nine of which had already been reported, although this was the first report for the tenth, S. octomerellum Hue, in Korea. General characteristics of Stereocaulon spp. include coralloid phyllocladia and tubercular cephalodia; however, the specimen first collected in Korea was a rare species with tomentum on the pseudopodetia. The specimen of S. octomerellum is characterized by the presence of a primary thallus, granule to short coralloid phyllocladia, and pseudopodetia up to 1 cm in size, with tubercular cephalodia. To determine the phylogeny of the specimens, we compared the ITS sequences of ribosomal DNA and the b-tubulin gene sequences. Phylogenetic analysis showed that the Korean Stereocaulon species were monophyletic and placed in the previous phylogenetic classification. Species of S. intermedium and S. exutum, however, were polyphyletic, and are morphologically variable and widespread species. Overall, we present here detailed morphological and chemical descriptions of each species identified and a revised key of all known Stereocaulon species in South Korea.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.23 no.3
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• pp.45-62
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• 2020

This study is to present the basic data for the conservation and management of natural resources of National Park by understanding the ecological characteristics of special protected areas located in Tohamsan District, Gyeongju National Park, South Korea. The numbers of vascular plants were summarized as 106 taxa including 47 families, 79 genera, 96 species, 1 subspecies, 7 varieties and 2 forms. For status by sites, there were 59, 50 and 55 taxa in A area(Pedicularis ishidoyana), B area(Millettia japonica) and C area(Cypripedium macranthos), respectively. In the dominant species, the tree layer was Quercus serrata(A area), Platycarya strobilacea and Pinus koraiensis(B area) and Fraxinus rhynchophylla(C area), the subtree layer was Carpinus cordata and Quercus serrata(A area), Pinus koraiensis and Castanea crenata(B area) and Fraxinus rhynchophylla(C area), the 1^st shrub layer was Toxicodendron trichocarpum and Rhododendron schlippenbachii(A area), Lindera erythrocarpa and Styrax japonicus(B area) and Fraxinus rhynchophylla and Lindera erythrocarpa(C area), 2^nd shrub layer was Styrax obassis and Lespedeza maximowiczii(A area), Lespedeza maximowiczii and Rhododendron mucronulatum(B area) and Lindera erythrocarpa(C area), the herbaceous layer was Athyrium yokoscense, Dryopteris chinensis, Dryopteris lacera and Lindera obtusiloba(A area), Athyrium yokoscense, Millettia japonica, Carex humilis Leyss. var. nana and Carex ciliato-marginata(B area) and Hydrangea macrophylla (Thunb.) Ser. subsp. serrata, Ajuga spectabilis and Oplismenus undulatifolius(C area).

• Journal of Embryo Transfer
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• v.32 no.2
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• pp.53-58
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• 2017

It is very difficult to get the information about semen quality analysis in transgenic pigs because of limited numbers and research facilities. Therefore, in the present study, we analyzed the semen quality of transgenic boars generated for xenotransplantation research. Briefly, the semen samples were collected from 5 homozygous ${\alpha}1,3$-Galactosyltransferase knock-out ($GalT^{-/-}$) transgenic boars and immediately transported to the laboratory. These semen samples were decupled with DPBS and conducted to analyze semen parameters by a computer-assisted semen analysis (CASA) system. The boar semen were examined all 12 parameters such as total motility (TM), curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), and hyperactivated (HYP), etc. In results, among the 5 $GalT^{-/-}$ boars, three boars (#134, 144, and 170) showed normal range of semen parameters, but #199 and 171 boars showed abnormal ranges of semen parameters according to standard ranges of semen parameters. Unfortunately, #171 boar showed azoospermia symptom with rare sperm counts in the original semen. Conclusively, assessment of semen parameters by CASA system is useful to pre-screening of reproductively healthy boar prior to natural mating and artificial insemination for multiplication and breeding.