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The Inhibitory Effect of Ecklonia cava and Eisenia bicyclis Ethanol Extract on Histamine in Mackerel (감태와 대황 에탄올 추출물 처리에 의한 저장 중 고등어 내의 히스타민 생성 억제 효과)

  • Kim, Bo-Ram;Kim, Koth-Bong-Woo-Ri;Kim, Min-Ji;Kim, Dong-Hyun;Jung, Seul-A;Kang, Bo-Kyeong;Bark, Si-Woo;Pak, Won-Min;Park, Hong-Min;Lim, Sung-Mee;Cho, Young-Je;Ahn, Dong-Hyun
    • Microbiology and Biotechnology Letters
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    • v.42 no.1
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    • pp.93-98
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    • 2014
  • This study was conducted to investigate the inhibitory effect of Ecklonia cava (EC) and Eisenia bicyclis (EB) ethanol extract on histamine production in mackerel. Changes in viable cell counts, histamine contents, pH and VBN of mackerel fillet treated with ethanol extracts during 25 days at 4 were measured. Treatments of EC and EB ethanol extract had reduced growth of viable cells by 2 log cycles during storage. Production of histamine was decreased by EC and EB extracts (115 and 96 ppm) when compared to the control at 5 days (384 ppm). The pH of mackerels treated with EC and EB extracts were no different, while the pH of the control increased during storage. Furthermore, the VBN of mackerels treated with EC and EB extracts were significantly decreased when compared to the control. In conclusion, EC and EB extract may reduce scombroid fish poisoning by decreasing histamine production in mackerel during refrigerated storage.

Comparative study on prevalence and components of metabolic syndrome and nutritional status by occupation and gender: Based on the 2013 Korea National Health and Nutrition Examination Survey (남녀별 직업군에 따른 대사증후군의 구성요소와 유병률 및 영양 상태의 비교 연구 : 2013년 국민건강영양조사 자료를 이용하여)

  • Kim, Ga Ram;Park, Hae Ryun;Lee, Young Mi;Lim, Young Suk;Song, Kyung Hee
    • Journal of Nutrition and Health
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    • v.50 no.1
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    • pp.74-84
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    • 2017
  • Purpose: In this study, factors of metabolic syndrome and nutritional status were examined according to gender and occupations using the 2013 Korea National Health and Nutrition Examination Survey (KNHANES). Methods: This study was conducted on 1,750 workers (male : 892, female : 858) aged between 30 and 64, who participated in a health survey, health examination, and nutrition survey using the 6th 2013 KNHANES. Occupations were classified into white collar and blue collar workers, and nutrient intake was analyzed using a food frequency questionnaire. Analysis of complex sample design data through SPSS 19.0 was used for analysis. Results: The prevalence rate of metabolic syndrome among blue collar (35.1%) was higher than that among white collar workers (26.8%) in male subjects (p < 0.05) as well as in blue collar (24.8%) compared to white collar workers (8.9%) in female subjects (p < 0.001). Intake frequency per week, considering one portion by food category, showed significant differences in cooked rice (p < 0.05) and bakeries and confectioneries (p < 0.05) in make workers as well as stew and casserole (p < 0.01) and fruits (p < 0.05) in female workers. With regard to nutrient intake by occupation and gender, white collar workers consumed a greater amount of nutrients (not including total energy intake) compared to blue collar workers in both male and female workers. With regard to nutrient adequacy ratio (NAR) and mean adequacy ratio (MAR) according to gender and occupation, white collar workers showed higher numbers than blue collar workers in both male and female subjects. Conclusions: This study examined the prevalence rates of metabolic syndrome and nutrient intake according to gender and occupation. In both male and female subjects, blue collar workers showed higher prevalence rates compared to white collar workers, and their diet quality was worse than white collar workers' diet quality. Considering this result, customized nutrition education according to gender and occupation should be provided to workers to prevent diseases.

EFFECT OF CROSS-SECTIONAL AREA OF 6 NICKEL-TITANIUM ROTARY INSTRUMENTS ON THE FATIGUE FRACTURE UNDER CYCLIC FLEXURAL STRESS: A FRACTOGRAPHIC ANALYSIS (반복 굽힘 스트레스 하에서 전동식 니켈-티타늄 파일의 단면적의 크기가 피로파절에 미치는 영향 : 파절역학 분석)

  • Hwang, Soo-Youn;Oh, So-Ram;Lee, Yoon;Lim, Sang-Min;Kum, Kee-Yeon
    • Restorative Dentistry and Endodontics
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    • v.34 no.5
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    • pp.424-429
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    • 2009
  • This study aimed to assess the influence of different cross-sectional area on the cyclic fatigue fracture of Ni-Ti rotary files using a fatigue tester incorporating cyclical axial movement. Six brands of Ni-Ti rotary files (ISO 30 size with. 04 taper) of 10 each were tested: Alpha system (KOMET), HeroShaper (MicroMega), K3 (SybronEndo), Mtwo (VDW), NRT (Mani), and ProFile (Dentsply). A fatigue-tester (Denbotix) was designed to allow cyclic tension and compressive stress on the tip of the instrument. Each file was mounted on a torque controlled motor (Aseptico) using a 1:20 reduction contra-angle and was rotated at 300 rpm with a continuous, 6 mm axial oscillating motion inside an artificial steel canal. The canal had a $60^{\circ}$ angle and a 5 mm radius of curvature. Instrument fracture was visually detected and the time until fracture was recorded by a digital stop watch. The data were analyzed statistically. Fractographic analysis of all fractured surfaces was performed to determine the fracture modes using a scanning electron microscope. Cross-sectional area at 3 mm from the tip of 3 unused Ni-Ti instruments for each group was calculated using Image-Pro Plus (Imagej 1.34n, NIH). Results showed that NRT and ProFile had significantly longer time to fracture compared to the other groups (p < .05). The cross-sectional area was not significantly associated with fatigue resistance. Fractographycally, all fractured surfaces demonstrated a combination of ductile and brittle fracture. In conclusion, there was no significant relationship between fatigue resistance and the cross-sectional area of Ni-Ti instruments under experimental conditions.

The Influences of Coastal Upwelling on Phytoplankton Community in the Southern Part of East Sea, Korea (동해 남부 연안 해역에서 냉수대 발생이 식물플랑크톤 군집에 미치는 영향)

  • Kim, A-Ram;Youn, Seok-Hyun;Chung, Mi-Hee;Yoon, Sang-Chol;Moon, Chang-Ho
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.19 no.4
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    • pp.287-301
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    • 2014
  • In order to understand environment condition and phytoplankton community before and after coastal upwelling, the influences of upwelling events on phytoplankton community were studied at 18 stations located the Southern part of East Sea, Korea from May to August 2013. The surface water masses showed low temperature and high salinity due to upwelling events at coastal stations (A1, B1, C1). Correlation between temperature and nutrients (DIP, r=-0.218, p<0.01; DIN, r=-0.306, p<0.01; silicate, r=-0.274, p<0.01) was significantly negative. This result could be explained that nutrients were supplied to surface water by the upwelling of bottom water. Phytoplankton communities were composed of 186 species. Phytoplankton abundance were relatively high in May (C1, $726{\times}10^3cells\;L^{-1}$) and July (A1, $539{\times}10^3cells\;L^{-1}$). Total chlorophyll a and micro-size fraction ($&gt;20{\mu}m$) increased at coastal stations in July and August, while phytoplankton abundance and total chl. a was much low in June. Dominant species in June was Pseudo-nitzschia spp. of which the cell size was $309{\mu}m^3$. Cell size of Pseudo-nitzschia spp. was smaller than dominant species in other period. Therefore, the increase in total chloro-phyll a and the size of phytoplankton was resulted in the sufficient supply of nutrients. In contrast, these tendencies were not observed at outside stations. These results suggested that coastal upwelling was an important influencing factor to determine the species composition and standing stock of phytoplankton community in the coastal waters of the Southern part of East Sea, Korea.

Levels of Plasma Glucose and Lipid in Rats Fed Bread Supplemented with Natural Extracts (천연추출물이 첨가된 식빵을 섭취한 흰쥐의 혈당 및 지질수준에 미치는 영향)

  • Kim, Se-Wook;Han, Ah-Ram;Chun, Su-Hyun;Nam, Mi-Hyun;Hong, Chung-Oui;Kim, Bok Hee;Kim, Tae Cheol;Lee, Kwang-Won
    • Korean Journal of Food Science and Technology
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    • v.48 no.1
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    • pp.77-85
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    • 2016
  • In this study, 4-week-old rats were fed bread supplemented with Terminalia chebula (TC), Plantago asiatica (PA), Linder obtusiloba (LO), and Capsosiphon fulvescens (CF) ethanol extracts, to determine the decrease in blood glucose levels, as well as the anti-inflammatory and lipid-enhancing effects. Previous studies have demonstrated the antioxidative effects of these ethanol extracts. After sacrifice, the liver tissue, whole blood, and serum samples were collected for biochemical analysis. The results showed a significant decrease in blood glucose level, lipid peroxidation, malondialdehyde (MDA) level, HbA1c level, total cholesterol, and low-density lipoprotein (LDL)-cholesterol (p<0.05) and an increase in high-density lipoprotein (HDL)-cholesterol level in rats fed bread supplemented with LO and CF ethanol extracts (p<0.05). Therefore, the results of this study demonstrate that bread supplemented with LO and CF ethanol extracts can potentially affect the blood glucose level and lead to lipid enhancement.

In vivo Study of the Renal Protective Effects of Capsosiphon fulvescens against Streptozotocin-induced Oxidative Stress (스트렙토조토신 유발 당뇨 쥐의 산화스트레스에 대한 매생이 추출물의 신장 보호 효과)

  • Nam, Mi-Hyun;Koo, Yun-Chang;Hong, Chung-Oui;Yang, Sung-Yong;Kim, Se-Wook;Jung, Hye-Lim;Lee, Hwa;Kim, Ji-Yeon;Han, Ah-Ram;Son, Won-Rak;Pyo, Min-Cheol;Lee, Kwang-Won
    • Korean Journal of Food Science and Technology
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    • v.46 no.5
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    • pp.641-647
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    • 2014
  • In this study, we evaluated the effect of Capsosiphon fulvescens extract (CFE) and its active compound, pheophorbide A (PhA), on diabetic kidney failure. Diabetes mellitus (DM) was induced by a single intraperitoneal injection of streptozotocin (STZ; 40 mg/kg body weight (BW)). After a week, the rats were orally administered CFE (4 and 20 mg/kg BW) or PhA (0.2 mg/kg BW) once a day for 9 weeks. After scarification, renal tissue samples were collected for biochemical and histochemical analyses. Our study showed that the treatment with CFE and PhA significantly decreased lipid peroxidation level and the activities of glutathione peroxidase and glutathione-S-transferase (p<0.05), but it increased glutathione level and the activities of glutathione reductase, superoxide dismutase, and catalase in the renal tissues (p<0.05). The CFE- and PhA-treated rats with DM showed improved histochemical appearance and decreased abnormal glycogen accumulation. Therefore, we suggest that PhA-containing CFE could exert renal protective effects against STZ-induced oxidative stress.

Anti-Atopic Activity of Tuna Heart Ethanol Extract (참치심장 에탄올 추출물의 항아토피 효과)

  • Kang, Bo-Kyeong;Kim, Koth-Bong-Woo-Ri;Kim, Min-Ji;Bark, Si-Woo;Pak, Won-Min;Kim, Bo-Ram;Ahn, Na-Kyung;Choi, Yeon-Uk;Bae, Nan-Young;Park, Ji-Hye;Ahn, Dong-Hyun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.1
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    • pp.1-6
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    • 2015
  • Atopic dermatitis (AD) is a form of allergic skin inflammatory characterized by late eczematous skin lesions. The incidence of AD is increasing, and it causes problems with administrative costs. Therefore, development of an AD treatment with no side effects is needed. The purpose of this study was to evaluate tuna heart ethanol extract (THEE), a functional extract from by-product of tuna. AD was induced by spreading 2,4-dinitrochlorobenzene (DNCB) on the backside of BALB/c mice. The effect of THEE was tested by measuring skin clinical severity score, secretion of cytokines and IgE, and proliferation. Secretion of $TNF-{\alpha}$, IL-4, IL-5, IL-13, and IgE significantly decreased in a THEE-independent manner. In contrast, levels of IL-10 and $IFN-{\gamma}$ significantly increased in mice sera and splenocytes. In addition, THEE alleviated AD symptoms compared to the DNCB only group. In conclusion, these results demonstrate that THEE has an inhibitory effect on AD and may be a useful substance for the development of cosmeceuticals.

The impact of exposure to peer delinquency in elementary school students and the mediating effect of aggression: Comparison between male and female elementary school students (또래집단의 비행경험이 초등학생 비행경험에 미치는 영향: 공격성의 매개효과를 중심으로 -남녀 초등학생 비교-)

  • Lee, Sang Hoon;Choi, Bo Ram;Kim, Sung Hee;Jeong, Kyu Hyoung
    • Journal of the Korean Society of Child Welfare
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    • no.58
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    • pp.205-229
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    • 2017
  • The purpose of this study was to examine gender differences in the impact of exposure to peer delinquency among elementary school-age students and the mediating effects of aggression. The study utilized 458 cases (220 male students, 238 female students) of data from the 2015 Korea Welfare Panel Study (KoWePS) conducted by the Korea Institute for Health and Social Affairs (KIHASA). The theoretical frameworks used in this study included Bandura's social learning theory, Akers' social learning theory, and Sutherland's differential association theory. The findings were as follows. First, there was no statistically significant effect on peer group's delinquency experience overall, aggression, and delinquency experience by gender. Second, male students' delinquency experience of their peer group had a statistically significant effect on their delinquency, however, this was not true for female students. Third, in the case of male students, aggression was found to mediate the relationship between peer group delinquency experience and their own delinquency, but not for female students. From these findings, we suggest a practical and policy-driven intervention plan, focusing on reducing the contact frequency of delinquency experience and aggression, The purpose of this study was to examine gender differences in the impact of exposure to peer delinquency among elementary school-age students and the mediating effects of aggression. The study utilized 458 cases (220 male students, 238 female students) of data from the 2015 Korea Welfare Panel Study (KoWePS) conducted by the Korea Institute for Health and Social Affairs (KIHASA). The theoretical frameworks used in this study included Bandura's social learning theory, Akers' social learning theory, and Sutherland's differential association theory. The findings were as follows. First, there was no statistically significant effect on peer group's delinquency experience overall, aggression, and delinquency experience by gender. Second, male students'delinquency experience of their peer group had a statistically significant effect on their delinquency, however, this was not true for female students. Third, in the case of male students, aggression was found to mediate the relationship between peer group delinquency experience and their own delinquency, but not for female students. From these findings, we suggest a practical and policy-driven intervention plan, focusing on reducing the contact frequency of delinquency experience and aggression, which was found to adversely affect elementary school students' delinquency.

Effects of Refrigerated Storage Temperature and Duration on the Seedling Quality of Bare Root Plants and Container Seedlings of Quercus variabilis and Zelkova serrata (저장 온도 및 기간이 굴참나무와 느티나무 노지묘 및 용기묘의 묘목품질에 미치는 영향)

  • Cho, Min Seok;Yang, A-Ram;Noh, Nam Jin
    • Journal of Korean Society of Forest Science
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    • v.110 no.3
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    • pp.406-418
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    • 2021
  • This study was conducted to evaluate optimal storage techniques for bare root plants and container seedlings of Quercus variabilis and Zelkova serrata in order to maintain high quality of seedlings until planting. Refrigerated storage treatments were given at two temperatures (-2℃ [freezing] and 2℃ [cooling]) for nine different durations (0, 15, 30, 60, 120, 180, 240, 300, and 360 days after storage). We analyzed total nonstructural carbohydrate (TNC) content and measured shoot moisture content (SMC) during the storage stage and survival rate (SR) and dry weight during the planting stage of seedlings. The TNC content and SMC of the seedlings of the two species decreased with an increase in storage duration. The TNC content of seedlings rapidly decreased after 180~240 days of storage. The TNC reduction rate in the freezing treatment was lower than that in the cooling treatment. Also, with an increase in the storage duration of the two species, the SMC reduction rate in the cooling treatment increased in comparison with that in the freezing treatment. In both the species, the SR after planting decreased rapidly after 60 days of cooling storage and 180 days of freezing storage, respectively. The SR after planting was less than 60% when the TNC content for both the species dropped below 20 mg g-1. In addition, the SR was lower than 80% when SMC measured before storage decreased by approximately 30% and 20% for Q. variabilis and Z. serrata, respectively. Our results suggest that cooling (1~2℃) storage is recommended for a short-term period (2 months or less), whereas freezing (-2~-4℃) storage is suitable for longer periods (2~6 months). These optimal storage techniques, allied with seedling harvesting and handling systems, will improve the quality of seedling production in nursery stages and increase seedling growth performances in plantations.

Change Detection for High-resolution Satellite Images Using Transfer Learning and Deep Learning Network (전이학습과 딥러닝 네트워크를 활용한 고해상도 위성영상의 변화탐지)

  • Song, Ah Ram;Choi, Jae Wan;Kim, Yong Il
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.37 no.3
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    • pp.199-208
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    • 2019
  • As the number of available satellites increases and technology advances, image information outputs are becoming increasingly diverse and a large amount of data is accumulating. In this study, we propose a change detection method for high-resolution satellite images that uses transfer learning and a deep learning network to overcome the limit caused by insufficient training data via the use of pre-trained information. The deep learning network used in this study comprises convolutional layers to extract the spatial and spectral information and convolutional long-short term memory layers to analyze the time series information. To use the learned information, the two initial convolutional layers of the change detection network are designed to use learned values from 40,000 patches of the ISPRS (International Society for Photogrammertry and Remote Sensing) dataset as initial values. In addition, 2D (2-Dimensional) and 3D (3-dimensional) kernels were used to find the optimized structure for the high-resolution satellite images. The experimental results for the KOMPSAT-3A (KOrean Multi-Purpose SATllite-3A) satellite images show that this change detection method can effectively extract changed/unchanged pixels but is less sensitive to changes due to shadow and relief displacements. In addition, the change detection accuracy of two sites was improved by using 3D kernels. This is because a 3D kernel can consider not only the spatial information but also the spectral information. This study indicates that we can effectively detect changes in high-resolution satellite images using the constructed image information and deep learning network. In future work, a pre-trained change detection network will be applied to newly obtained images to extend the scope of the application.