Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-Atopic Activity of Tuna Heart Ethanol Extract

참치심장 에탄올 추출물의 항아토피 효과

  • Kang, Bo-Kyeong (Department of Food Science & Technology/Institute of Food Science, Pukyoung National University) ;
  • Kim, Koth-Bong-Woo-Ri (Institute of Fisheries Sciences, Pukyoung National University) ;
  • Kim, Min-Ji (Institute of Fisheries Sciences, Pukyoung National University) ;
  • Bark, Si-Woo (Department of Food Science & Technology/Institute of Food Science, Pukyoung National University) ;
  • Pak, Won-Min (Department of Food Science & Technology/Institute of Food Science, Pukyoung National University) ;
  • Kim, Bo-Ram (Department of Food Science & Technology/Institute of Food Science, Pukyoung National University) ;
  • Ahn, Na-Kyung (Department of Food Science & Technology/Institute of Food Science, Pukyoung National University) ;
  • Choi, Yeon-Uk (Department of Food Science & Technology/Institute of Food Science, Pukyoung National University) ;
  • Bae, Nan-Young (Department of Food Science & Technology/Institute of Food Science, Pukyoung National University) ;
  • Park, Ji-Hye (Department of Food Science & Technology/Institute of Food Science, Pukyoung National University) ;
  • Ahn, Dong-Hyun (Department of Food Science & Technology/Institute of Food Science, Pukyoung National University)
  • 강보경 (부경대학교 식품공학과/식품연구소) ;
  • 김꽃봉우리 (부경대학교 수산과학연구소) ;
  • 김민지 (부경대학교 수산과학연구소) ;
  • 박시우 (부경대학교 식품공학과/식품연구소) ;
  • 박원민 (부경대학교 식품공학과/식품연구소) ;
  • 김보람 (부경대학교 식품공학과/식품연구소) ;
  • 안나경 (부경대학교 식품공학과/식품연구소) ;
  • 최연욱 (부경대학교 식품공학과/식품연구소) ;
  • 배난영 (부경대학교 식품공학과/식품연구소) ;
  • 박지혜 (부경대학교 식품공학과/식품연구소) ;
  • 안동현 (부경대학교 식품공학과/식품연구소)
  • Received : 2014.11.03
  • Accepted : 2014.12.30
  • Published : 2015.01.31
Download PDF
⟨ Previous Next ⟩

Abstract

Atopic dermatitis (AD) is a form of allergic skin inflammatory characterized by late eczematous skin lesions. The incidence of AD is increasing, and it causes problems with administrative costs. Therefore, development of an AD treatment with no side effects is needed. The purpose of this study was to evaluate tuna heart ethanol extract (THEE), a functional extract from by-product of tuna. AD was induced by spreading 2,4-dinitrochlorobenzene (DNCB) on the backside of BALB/c mice. The effect of THEE was tested by measuring skin clinical severity score, secretion of cytokines and IgE, and proliferation. Secretion of $TNF-{\alpha}$, IL-4, IL-5, IL-13, and IgE significantly decreased in a THEE-independent manner. In contrast, levels of IL-10 and $IFN-{\gamma}$ significantly increased in mice sera and splenocytes. In addition, THEE alleviated AD symptoms compared to the DNCB only group. In conclusion, these results demonstrate that THEE has an inhibitory effect on AD and may be a useful substance for the development of cosmeceuticals.

본 연구는 DNCB(2,4-dinitrochlorobenzene) 도포를 통해 아토피 피부염을 유발시킨 BALB/c mice에 참치심장 에탄올 추출물(THEE)을 2주 동안 지속적으로 처리하여 THEE의 아토피 피부염 완화 효과에 대해 연구하였다. 이를 위해 Th1 및 Th2 cytokines의 분비량, 비장 세포 증식능, 혈청중 총 IgE 함량, 육안 평가 및 skin clinical severity score를 실시하였다. 그 결과 THEE를 지속적으로 도포함으로써 혈청 내 총 IgE의 분비량이 현저히 감소함을 나타내었다. Th1 세포로부터 생성되는 $IFN-{\gamma}$ cytokine은 증가하고 염증매개성 cytokine인 $TNF-{\alpha}$의 분비량은 감소함을 확인하였다. 또한 Th2 cytokine에 속하는 IL-4, IL-5, IL-13 분비량은 감소하였으며 항염증성 cytokine인 IL-10의 분비량이 증가함을 보여 항아토피 효과를 나타내었다. 육안 평가를 통해서 THEE를 2주간 지속적으로 도포 처리하였을 때 그 증상이 눈에 띄게 완화되는 것을 확인할 수 있었으며, skin clinical severity score에서도 DNCB 단독 처리군과 비교시 유의적으로 그 값이 감소하였다. 이상의 결과들로부터 THEE는 Th1과 Th2 cytokine의 생성 및 활성을 조절하고 IgE의 과다 발현을 억제함으로써 아토피 피부염의 개선에 뛰어난 효능을 가지고 있는 것으로 사료된다.

Keywords

References

  1. Leung DYM, Boguniewicz M, Howell MD, Nomura I, Hamid QA. 2004. New insights into atopic dermatitis. J Clin Invest 113: 651-657. https://doi.org/10.1172/JCI21060
  2. Ahn KM. 2004. Role of mast cells in allergic inflammation and innate immunity. Korean J Pediatr 47: 1137-1141.
  3. Epstein PR. 2005. Climate change and human health. N Engl J Med 353: 1433-1436. https://doi.org/10.1056/NEJMp058079
  4. Leung DYM. 1999. Pathogenesis of atopic dermatitis. J Allergy Clin Immunol 104: S99-108. https://doi.org/10.1016/S0091-6749(99)70150-8
  5. Kevin DC. 1994. Atopic dermatitis: recent trends in pathogenesis and therapy. J Invest Dermatol 102: 128-137. https://doi.org/10.1111/1523-1747.ep12371746
  6. Novak N, Simon D. 2011. Atopic dermatitis-from new pathophysiologic insights to individualized therapy. Allergy 66: 830-839. https://doi.org/10.1111/j.1398-9995.2011.02571.x
  7. Sidbury R, Hanifin JM. 2000. Old, new, and emerging therapies for atopic dermatitis. Dermatol Clin 18: 1-11.
  8. Choi J, Kim JH, Lee JW. 2011. Physiological properties of tuna cooking drips hydrolysate prepared with gamma irradiation. Process Biochem 46: 1875-1878. https://doi.org/10.1016/j.procbio.2011.06.005
  9. Je JY, Qian ZJ, Byun HG, Kim SK. 2007. Purification and characterization of an antioxidant peptide obtained from tuna backbone protein by enzyme hydrolysis. Process Biochem 42: 840-846. https://doi.org/10.1016/j.procbio.2007.02.006
  10. Hwang WI, Baik NG, Hwang YK, Lee SD. 1992. Antitumor and immunological effects of tuna extract. J Korean Soc Food Nutr 21: 353-366.
  11. Jung HS. 2007. Antioxidant effect of histidine containing low molecular weight peptide isolated from skipjack boiled extract. Korean J Food Cookery Sci 23: 221-226.
  12. Shin MO, Ku MJ, Bae SJ. 2007. Cytotoxicity and quinone reductase activity stimulating effects of fin of Thunnus thynnus extracts in various cancer cells. Korean J Nutr 40: 147-153.
  13. Park SE, Kim HW, Lee SR, Kim BK. 2000. Effects of nucleic acids complex of tuna testis on immunological activities. J Korean Assoc Cancer Prev 5: 15-23.
  14. Ahn JY, Im LR, Park JH, Kim DH, Lee MY. 2009. Effects of Rumecis Radix water extract on development of atopic dermatitis in BALB/c mice. Kor J Pharmacogn 40: 218-223.
  15. Renz H, von Mutius E, Illi S, Wolkers F, Hirsch T, Weiland SK. 2002. T(H)1/T(H)2 immune response profiles differ between atopic children in eastern and western Germany. J Allergy Clin Immunol 109: 338-342. https://doi.org/10.1067/mai.2002.121459
  16. Suzuki R, Shimizu T, Kudo T, Ohtsuka Y, Yamashiro Y, Oshida K. 2002. Effects of n-3 polyunsaturated fatty acids of dermatitis in NC/Nga mice. Prostaglandins Leukot Essent Fatty Acids 66: 435-440. https://doi.org/10.1054/plef.2002.0370
  17. Munoz C, Carlet J, Fitting C, Misset B, Blériot JP, Cavaillon JM. 1991. Dysregulation of in vitro cytokine production by monocytes during sepsis. J Clin Invest 88: 1747-1754. https://doi.org/10.1172/JCI115493
  18. Lee YH. 2007. Effect of Phellinus linteus grown in germinated brown rice on atopic dermatitis. J Kor Soc Cosm 13: 514-519.
  19. Powrie F, Coffman RL. 1993. Cytokine regulation of T-cell function: potential for therapeutic intervention. Trends Pharm Sci 14: 164-168. https://doi.org/10.1016/0165-6147(93)90202-U
  20. Novak N, Bieber T. 2003. Allergic and nonallergic forms of atopic diseases. J Allergy Clin Immunol 112: 252-262. https://doi.org/10.1067/mai.2003.1595
  21. Beltrani VS. 1999. The clinical spectrum of atopic dermatitis. J Allergy Clin Immunol 104: S87-98. https://doi.org/10.1016/S0091-6749(99)70050-3
  22. Smith JR, Hart PH, Williams KA. 1998. Basic pathogenic mechanisms operating in experimental models of acute anterior uveitis. Immunol Cell Biol 76: 497-512. https://doi.org/10.1046/j.1440-1711.1998.00783.x
  23. Moore KW, O'Garra A, de Waal Malefyt R, Vieira P, Mosmann TR. 1993. Interleukin-10. Annu Rev Immunol 11: 165-190. https://doi.org/10.1146/annurev.iy.11.040193.001121
  24. Gonzalez-Rey E, Chorny A, Delgado M. 2007. Regulation of immune tolerance by anti-inflammatory neuropeptides. Nat Rev Immunol 7: 52-63. https://doi.org/10.1038/nri1984

Cited by

  1. Anti-wrinkle effects of a tuna heart H2O fraction on Hs27 human fibroblasts vol.37, pp.1, 2016, https://doi.org/10.3892/ijmm.2015.2407
  2. Anti-Atopic Effects of Mixed Extracts from Date Plum, Persimmon, and Mulberry Leaves vol.45, pp.4, 2016, https://doi.org/10.3746/jkfn.2016.45.4.501
  3. Anti-atopic Effect of Hot Water and Supercritical Carbon Dioxide Fluid Extract of Persimmon (Diospyros kaki) Peels vol.47, pp.3, 2015, https://doi.org/10.9721/KJFST.2015.47.3.394
  4. Study of Anti-atopic Dermatitis Effects of Juice of Raphanus sativus var in HaCaT Cell Line vol.32, pp.4, 2017, https://doi.org/10.7841/ksbbj.2017.32.4.311
  5. Anti-inflammatory effects of Dendropanax morbifera in lipopolysaccharide-stimulated RAW264.7 macrophages and in an animal model of atopic dermatitis vol.19, pp.3, 2019, https://doi.org/10.3892/mmr.2019.9887