• Korean Journal of Microbiology
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• v.33 no.2
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• pp.131-135
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• 1997

Several microorganisms capable of producing protopectinase, which catalyzes dissociation of plant tissue to single cells, were isolated from soils. Crude enzymes prepared from culture supernatants of the strains released potato cells as a single cell from potato tissues. One of the isolated strains showing higher activity of protopectinase was selected and identified as Rhizopus sp. from the morphological characteristics. For preparing single cells from potato tissues, optimum enzyme activity of protopectinase, which was prepared from culture filtrate of Rhizopus sp. R2, was obescrved at $40^{\circ}C$ and pH 4.

• Journal of Korean Institute of Industrial Engineers
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• v.40 no.1
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• pp.118-127
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• 2014

The configuration such as series, parallel and k-out-of-n of a repairable system directly affects its reliability. The maintenance strategy can also affect the overall performance of the system. The objective of this work is to investigate the possible trade-off between the configuration of a repairable k-out-of-n system and its maintenance strategy. The redundancy is considered to be the design decision variables, whereas the preventive maintenance period is considered to be the maintenance decision variables. The optimization model is used to minimize the overall life cycle cost associated with the system, considering constraint on reliability. Finally, genetic algorithm is used to find the optimal values for the decision variables. The result is compared with optimal values for considering redundancy and maintenance respectively.

• Proceedings of the SAREK Conference
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• 2006.06a
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• pp.352-356
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• 2006

As a basic study to develop heating panel, the purpose of this study was to know possibility as a floor heating panel. We investigated working conditions and performance of pulsating heat pipe. Tests were conducted under the next conditions; Working fluid were R-22 and R-134a, charging ratio 40% and 50%, temperature of inlet water $60^{\circ}C$ and $70^{\circ}C$, flow rates $1{\sim}3kg/min$. The experimental results indicate that the pulsating heat pipe charged 50% showed better performance than 40%, R-22 is more suited to the working fluid than R-134a, and it has a possibility which can be applied to floor heating panel using a pulsating heat pipe.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.34 no.3
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• pp.175-181
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• 2008

Cellolose nanoparticles loaded with retinyl palmitate were prepared by modified spontaneous emulsification solvent diffusion method. We used polysorbate 20, polysorbate 60, and PPG-26-Buteth-26/PEG-40 Hydrogenated castor oil as dispersion medium. The optimum condition for particle size of cellulose nanoparticles was 1w/v% ethyl cellulose with, 3w/v% polysorbate 60 solution. And The optimum condition for leading amount of retinyl palmitate of cellulose nanoparticles was 2w/v% ethyl cellulose with 1w/v% polysorbate 60 solution. Also, we found that this optimum condition can be applicable to other active compounds.

• Clean Technology
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• v.13 no.2
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• pp.151-158
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• 2007

An experimental study to investigate the condensing heat transfer characteristics of small diameter horizontal double pipe heat exchangers with R-22 and R-410A was performed. Experimental facility was constructed to calculate and observe HTC(heat transfer coefficients), flow patterns and pressure drop. The main components include a liquid pump, an evaporator, a condenser(test section), a sight-glass, pressure taps and measurement apparatus. Two pipes of different diameters are tested; One 5.35 mm ID 0.5 mm thick, the other 3.36 mm ID 0.7 mm thick. The mass flow rate ranged from 200 to $500\;ks/m^2{\cdot}s$ and heating capacity were form 1.0 to 2.4 kW. The flow patterns of R-22 and R-410A were observed with a high speed camera through the sight-glass. The tests revealed that HTC of R-410A was higher than that of R-22 by maximum 5%. Annular pattern was observed for the most cases but stratified flow was also detected when x<0.2. The pressure drop in 3.36 mm ID pipe was higher than that of 5.35 mm by $30{\sim}50%$. Comparing with previous correlations such as Shah, Fujii and Soliman's, Fujii' showed the best good agreement with my data with a maximum deviation of 40%.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.40 no.4
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• pp.213-220
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• 2016

The objective of this study is to investigate the cooling performance of an air-conditioning system for a special purpose vehicle under tropical and severe weather conditions. In order to evaluate and compare the cooling performances, the dual refrigeration cycle using R-134a was tested on a special purpose vehicle with various refrigerant charge amounts and indoor temperatures. The cycle was tested considering indoor cooling speed and compression ratio (discharge pressure), and was optimized at the refrigerant charge amount of 1.5 kg and outdoor temperature of $40.0^{\circ}C$. The time to reach indoor temperature of $15.0^{\circ}C$ increased by 86.5% and 38.1%, at the indoor temperatures from $25.0^{\circ}C$ to $32.5^{\circ}C$ and from $32.5^{\circ}C$ to $40.0^{\circ}C$, respectively. In addition, with the increase in indoor temperature from $25.0^{\circ}C$ to $40.0^{\circ}C$, the cooling capacity increased by 7.3%, from 19.1 kW to 20.5 kW, but decreased by 7.0% from 4.67 to 5.1.

• Journal of Microbiology
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• v.44 no.1
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• pp.35-41
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• 2006

The unique gene encoding thioredoxin reductase (TrxR) was previously cloned and characterized from the fission yeast Schizosaccharomyces pombe, and its expression was induced by oxidative stress. To elucidate tbe regulatory mechanism of the S. pombe TrxR gene, three fusion plasmids were generated using polymerase chain reaction: pYUTR20, pYUTR30, and pYUTR40. Plasmid pYUTR20 has an upstream region of 891 base pairs, pYUTR30 has 499 in this region, and pYUTR40 has an 186 bp upstream region. Negatively acting sequence is located between $-1,526\;\~\;-891bp$ upstream of the gene. The upstream sequence, responsible for the induction of TrxR by menadione (MD), is situated on the $-499\;\~\;-186bp$ region, which is also required for TrxR induction by mercuric chloride. The same region also appeared to be required for Pap1-mediated transcriptional regulation of the TrxR gene, which contains the two plausible Papl binding sites, TTACGAAT and TTACGCGA. Consistently, basal and inducible expression of the TrxR gene was markedly lower in the Pap1-negative TP108-3C cells than in wild-type yeast cells. In summary, up-regulation of the S. pombe TrxR gene is mediated by Pap1 via the transcriptional motif(s) located on the $-499\;\~\;-186bp$ region.

• Journal of the Korean Institute of Oriental Medical Informatics
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• v.14 no.2
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• pp.73-79
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• 2008

Objectives : We investigated the correlation of the skin color to blood circulation on the face of Korean female by noninvasive-skin measuring devices. Methods : Korean female(37.47${\pm}$3.66 years, n=36) aged 30-45 years were participated in this study. We measured the skin color of the face with Spectrophotometer CM2600d (Minolta, Japan) and the skin blood flow with Laser Doppler Imaging-Periscan PIM II (Perimed AB, Sweden). The skin color and blood circulation of three groups (30-35 years, 36-40 years, 41-45 years) divided on age were analyzed by paired-t test and regression analysis. Results : The L* values of the skin were decreased and the a* values were increased significantly with age. This means that the more age increase, the more the skin is dark and red. But the skin blood flow was not significantly different with age and skin color. Conclusions : In the oriental medicine, It was reported that the face of the female over 35 years is changed gradually haggard and the blood circulation is decreased. In our study, the blood circulation was not changed and the skin color was dark and red with increasing the age. We think this symptom resulted from the better nutrition than the past, the change of the blood-quality and this changes were too sensitive to be measured by Laser Doppler Imaging system.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2000.04a
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• pp.206-209
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• 2000

Groove pressing which is analogous to groove rolling in the aspect of deformation mode was designed and influence of the uncommon shear deformation on the development of texture and R value was investigated. Texture developed by the groove pressing were measured as well as predicted. It was found out that the main component in the developed texture was {40。, 45。, 0。} in ODF which was regarded as a rotated Bs component and rarely observed in a plain rolling. The maximum R value was predicted to be 3.8 in 45。 direction which might be attributed to the new component.

• Proceedings of the KSR Conference
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• 1999.05a
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• pp.33-40
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• 1999

Park and Ride(P＆R) system has not implemented it's intended object in Seoul metropolitan area, still less it didn't impact on diminishing the ridership of urban railway. This paper is focused on analysis of Park and Rail ride user survey of travel behavior and trip chain. We propose the ideal location of P＆R in Seoul and stratagies to increase the utilization of P＆R.