• Natural Product Sciences
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• v.16 no.1
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• pp.26-31
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• 2010

High performance liquid chromatographic method with diode-array detection (HPLC-DAD) has been performed for the simultaneous determination of four marker constituents, paeoniflorin, trans-cinnamic acid, schisandrin and glycyrrhizin in traditional herbal medicinal preparation, So-Cheong-Ryong-Tang (SCRT). The presence of paeoniflorin, trans-cinnamic acid, schisandrin and glycyrrhizin in this decoction was ascertained by retention time, spiking with each authentic standard, UV spectrum and ESI mass spectrum. All four compounds showed good linearity ($r^2$ > 0.998) in a relatively wide concentration ranges. The RSD for intra-day and inter-day precision was less than 3% and the limits of detection (LOD) were less than 30 ng. The mean recovery of each compound was 94.1-113.0% with RSD values less than 3.0%. These results suggest that the developed HPLC method is simple, effective and could be readily utilized as a quality control method for commercial SCRT products.

• The Plant Pathology Journal
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• v.31 no.4
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• pp.402-413
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• 2015

Yellow rust (stripe rust), caused by Puccinia striiformis f. sp. tritici, is one of the most destructive foliar diseases of wheat in Egypt and worldwide. In order to identify wheat genotypes resistant to yellow rust and develop molecular markers associated with the resistance, fifty F8 recombinant inbred lines (RILs) derived from a cross between resistant and susceptible bread wheat landraces were obtained. Artificial infection of Puccinia striiformis was performed under greenhouse conditions during two growing seasons and relative resistance index (RRI) was calculated. Two Egyptian bread wheat cultivars i.e. Giza-168 (resistant) and Sakha-69 (susceptible) were also evaluated. RRI values of two-year trial showed that 10 RILs responded with RRI value >6 <9 with an average of 7.29, which exceeded the Egyptian bread wheat cultivar Giza-168 (5.58). Thirty three RILs were included among the acceptable range having RRI value >2 <6. However, only 7 RILs showed RRI value <2. Five RILs expressed hypersensitive type of resistance (R) against the pathogen and showed the lowest Average Coefficient of Infection (ACI). Bulked segregant analysis (BSA) with eight simple sequence repeat (SSR), eight sequence-related amplified polymorphism (SRAP) and sixteen random amplified polymorphic DNA (RAPD) markers revealed that three SSR, three SRAP and six RAPD markers were found to be associated with the resistance to yellow rust. However, further molecular analyses would be performed to confirm markers associated with the resistance and suitable for marker-assisted selection. Resistant RILs identified in the study could be efficiently used to improve the resistance to yellow rust in wheat.

• Journal of the Korean Wood Science and Technology
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• v.36 no.3
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• pp.9-16
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• 2008

Ribbed birch (Betula costata Trautv.), Japanese larch (Larix kaempferi (Lamb.) Carr.), and tulip tree (Liriodendron tulipifera L.), were used as experimental specimens to measure the sound absorption coefficient with various resonator types, regular resonator (Type-R), eccentric resonator (Type-E), aligned resonator (Type-A), and screwed resonator (Type-S). Resonators consisted of the simple perforation by hand drilling. Sound absorption performances of the resonators installed perforations were better than those of untreated specimens. They were varied with the resonator's type and wood species. Increased area by a wood screw gave no significant change on the sound absorption.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2005.05a
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• pp.458-462
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• 2005

This paper deal with the optimal lamination condition of cylindrical shell applied new composite URN300 for a study of composite empirical formula. Finite element analyses for isotropic materials considered element numbers and boundary conditions are compared with existing empirical formulas to apply FE analysis for composite. And composite tensile test is done to know the composite material applied FE analysis for composite. The results of FE analyses for isotropic materials have indicated that Optimal element number and boundary condition were 1600 and both simple support. These conditions were applied in composite FE analyses. Ply orientations and lamination patterns in FE analyses for composite were considered. Ply orientations are $0^{\circ},\;15^{\circ},\;30^{\circ},\;45^{\circ},\;60^{\circ},\;75^{\circ},\;and\;90^{\circ}$. Lamination patterns are $[\pm\theta/0/90]_{14s]$ and $[\pm\theta_{14}/0_{14}/90_{14}]_s$ in FE analysis. Lamination pattern $[\pm\theta_{14}/0_{14}/90_{14}]_s$ is the equivalent model of $[\pm\theta/0/90]_{14s}$. At the result of this study, the FE analyses for composite have indicated that the optimized ply orientation $75^{\circ}$ is and real model must use in FE analysis for accurate results.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2005.05a
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• pp.467-470
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• 2005

There are about 10 motors for tile actuator of the automation system in an auto-mobile recently. The performance of the motor-case is much related to the noise and the vibration of an auto-mobile Multi-Forming process is so much the better than existing deep-drawing or Multi-step forming by press by less cost, installation and staff. But there isn't the specific and general process design, so we aren't good at competition. So in the first step, I want to study about the core design for the multi-forming process. We can access by the elasto-plastic theory and the finite element method, and we use a commercial package of the Deform-2D and, Deform-3D which is based on three-dimensional elasto-plastic finite element, evaluated propriety oi the package. The evaluation of the package propriety was simulated by simple bending example. It was found the elasto-plastic theory was mostly in agreement with the simulation. We proposed that three type of section for the core and analyzed by finite element method (Deform-2D). We can get the best result with the ellipse type core. Then we apply the result of the preceding analysis to the finite element method (Deform-3D). In 3D-finite element analysis, we can get the result of 8/100mm-roundness. This result can help the improvement of the multi-forming process.

• Horticultural Science & Technology
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• v.31 no.1
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• pp.72-79
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• 2013

Inactivation of the gene coding for dihydroflavonol 4-reductase (DFR) is responsible for the color difference between red and yellow onions (Allium cepa L.). Two inactive DFR-A alleles, DFR-$A^{PS}$ and DFR-$A^{DEL}$, were identified in our previous study. A functional marker was developed on the basis of the premature stop codon that inactivated the DFR-$A^{PS}$ allele. A derived cleaved amplified polymorphic sequences (dCAPS) primer was designed to detect the single nucleotide polymorphism, an A/T transition, which produced the premature stop codon. Digested PCR products clearly distinguished the homozygous and heterozygous red $F_2$ individuals. Meanwhile, to develop a molecular marker for detection of the DFR-$A^{DEL}$ allele in which entire DFR-A gene was deleted, genome walking was performed and approximately 3 kb 5' and 3' flanking sequences of the DFR-$A^R$ coding region were obtained. PCR amplification using multiple primers binding to the extended flanking regions showed that more of the extended region of the DFR-A gene was deleted in the DFR-$A^{DEL}$ allele. A dominant simple PCR marker was developed to identify the DFR-$A^{DEL}$ allele using the dissimilar 3' flanking sequences of the DFR-A gene and homologous DFR-B pseudogene. Distribution of the DFR-$A^{PS}$ and DFR-$A^{DEL}$ alleles in yellow onion cultivars bred in Korea and Japan was surveyed using molecular makers developed in this study. Results showed predominant existence of the DFR-$A^{PS}$ allele in yellow onion cultivars.

• Korean Journal of Metals and Materials
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• v.56 no.11
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• pp.835-843
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• 2018

In this work, we report a delta rosette strain sensor based on highly stretchable silver nanowire (AgNW) percolation piezoresistors. The proposed rosette strain sensors were easily prepared by a facile two-step fabrication route. First, three identical AgNW piezoresistive electrodes were patterned in a simple and precise manner on a donor film using a solution-processed drop-coating of the AgNWs in conjunction with a tape-type shadow mask. The patterned AgNW electrodes were then entirely transferred to an elastomeric substrate while embedding them in the polymer matrix. The fabricated stretchable AgNW piezoresistors could be operated at up to 20% strain without electrical or mechanical failure, showing a maximum gauge factor as high as 5.3, low hysteresis, and high linearity ($r^2{\approx}0.996$). Moreover, the sensor responses were also found to be highly stable and reversible even under repeated strain loading/unloading for up to 1000 cycles at a maximum tensile strain of 20%, mainly due to the mechanical stability of the AgNW/elastomer composites. In addition, both the magnitude and direction of the principal strain could be precisely characterized by configuring three identical AgNW piezoresistors in a delta rosette form, representing the potential for employing the devices as a multidimensional strain sensor in various practical applications.

• Proceedings of the Korea Water Resources Association Conference
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• 2019.05a
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• pp.155-155
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• 2019

The Agricultural Policy/Environmental eXtender (APEX) models have been developed for assessing agricultural management efforts and their effects on soil and water at the field scale as well as more complex multi-subarea landscapes, whole farms, and watersheds. National Academy of Agricultural Sciences, Wanju, Korea, has modified a key component of APEX application, named APEX-Paddy for simulating water quality with considering appropriate paddy management practices, such as puddling and flood irrigation management. Calibration and validation are an anticipated step before any model application. Simple techniques are essential to assess whether or not a parameter should be adjusted for calibration. However, very few study has been done to evaluate the ability of APEX-Paddy to simulate the impact of multiple management scenarios on nutrients loss. In this study, the observation data from experimental fields at Iksan in South Kora was used in calibration and evaluation process during 2013-2015. The APEX auto- calibration tool (APEX-CUTE) was used for model calibration and sensitivity analysis. Four quantitative statistics, the coefficient of determination ($R^2$),Nash-Sutcliffe(NSE),percentbias(PBIAS)androotmeansquareerror(RMSE)were used in model evaluation. In this study, the hydrological process of the modified model, APEX-Paddy, is being calibrated and tested in predicting runoff discharge rate and nutrient yield. Field-scale calibration and validation processes are described with an emphasis on essential calibration parameters and direction regarding logical sequences of calibration steps. This study helps to understand the calibration and validation way is further provided for applications of APEX-Paddy at the field scales.

• Clinical and Experimental Reproductive Medicine
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• v.32 no.3
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• pp.287-293
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• 2005

Objective: To evaluate the usefulness of serum concentrations of macrophage migration inhibitory factor (MIF) of patients with ovarian cysts for differential diagnosis of endometrioama. Method: From Jan. 2003 to Dec. 2004, preoperative serum MIF levels were assessed in 28 women with endometrioma, 32 with benign epithelial tumor, 23 with functional and simple cysts, 22 with benign mature cystic teratoma, and 25 women without ovarian tumor as control. MIF levels were determined using an ELISA (Quantikine Human MIF immunoassay, R&D Systems, Inc., USA). Results: Mean MIF levels were higher in all groups with benign tumors than control (all p<0.01), but there was no significant difference between benign tumor groups (p=0.95). There was no significant correlation between MIF levels and tumor volume, body mass index (BMI) (p=0.635, 0.674 respectively) Serum MIF level had significant correlation with count of WBC and neutrophils (p=0.008, 0.024 respectively), but had no correlation with count of lymhocytes and monocytes (p=0.688, 0.294 respectively). Conclusions: This study showed a marked increase in MIF concentrations in the peripheral blood of patients with endometrioma, but there was no significant difference with other benign tumors. Serum MIF level had significant correlation with count of WBC and neutrophils. These suggest serum MIF level has no usefulness for differential diagnosis of endometrioma from other benign ovarian cysts.

• Steel and Composite Structures
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• v.19 no.2
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• pp.369-384
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• 2015

This work presents a free vibration analysis of functionally graded metal-ceramic (FG) beams with considering porosities that may possibly occur inside the functionally graded materials (FGMs) during their fabrication. For this purpose, a simple displacement field based on higher order shear deformation theory is implemented. The proposed theory is based on the assumption that the transverse displacements consist of bending and shear components in which the bending components do not contribute toward shear forces and, likewise, the shear components do not contribute toward bending moments. The most interesting feature of this theory is that it accounts for a quadratic variation of the transverse shear strains across the thickness, and satisfies the zero traction boundary conditions on the top and bottom surfaces of the beam without using shear correction factors. In addition, it has strong similarities with Euler-Bernoulli beam theory in some aspects such as equations of motion, boundary conditions, and stress resultant expressions. The rule of mixture is modified to describe and approximate material properties of the FG beams with porosity phases. By employing the Hamilton's principle, governing equations of motion for coupled axial-shear-flexural response are determined. The validity of the present theory is investigated by comparing some of the present results with those of the first-order and the other higher-order theories reported in the literature. Illustrative examples are given also to show the effects of varying gradients, porosity volume fraction, aspect ratios, and thickness to length ratios on the free vibration of the FG beams.