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siRNA Silencing EZH2 Reverses Cisplatin-resistance of Human Non-small Cell Lung and Gastric Cancer Cells

  • Zhou, Wen;Wang, Jian;Man, Wang-Ying;Zhang, Qing-Wei;Xu, Wen-Gui
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.6
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    • pp.2425-2430
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    • 2015
  • Clinical resistance to chemotherapeutic agents is one of the major hindrances in the treatment of human cancers. EHZ2 is involved in drug resistance and is overexpressed in drug-resistant cancer cell lines. In this study, we investigated the effects of EHZ2 on cisplatin -resistance in A549/DDP and AGS/DDP cells. EHZ2 mRNA and protein were found to be significantly overexpressed in A549/DDP and AGS/DDP cells, compared to parental cells. EHZ2 siRNA successfully silenced EHZ2 mRNA and protein expression. Proliferation was inhibited and drug resistance to cisplatin was improved. Flow cytometry showed that silencing of EHZ2 arrested A549/DDP and AGS/DDP cells in the G0/G1 phase, increasing apoptosis, rh-123 fluorescence intensity and caspase-3/8 activities. Silencing of EHZ2 also significantly reduced the mRNA and protein expression levels of cyclin D1 and MDR1,while up-regulating p15, p21, p27 and miR-218 in A549/DPP cells. Furthermore, silencing of EHZ2 also significantly increased the expression level of tumor suppressor factor miR-218. We also found down-regulating EHZ2 expression increased methylation in A549/DDP and AGS/DDP cells. This study demonstrates that drug resistance can be effectively reversed in human cisplatin-resistant lung and gastric cancer cells through delivery of siRNAs targeting EHZ2.

The Value of Ultrasonographic Endometrial Measurement in the Prediction of Pregnancy Outcome in In Vitro Fertilization (체외수정시술 주기에서 자궁내막발달과 착상에 관한 연구)

  • Kim, Sun-Haeng
    • Clinical and Experimental Reproductive Medicine
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    • v.20 no.2
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    • pp.117-123
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    • 1993
  • The condition of the endometrium is an important factor which may influence the success or failure in IVF-ET. This study was undertaken for evaluation of the value of endometrial growth as an early predictor for the success of IVF. Ultrasonographic endometrial measurement were performed in 43 IVF cycles that conceived, 101 cycles that did not with an IVF-ET There was no significant difference in the endometrial thickness and the serum concentration of estradiol in the pregnant versus nonpregnant group(10.4 vs. 9.9 mm: 2348 vs. 2017 pg/ml no hCG administration day). No correlation was found between the ultrasound image and serum estradiol levels around the time of hCG administration(r=0.54, p=0.13 no Day 2; r=0.45, p=0.14 no Day 1). The duration of gonadotropin treatment, number of follicles, number of oocytes retrieved, and fertilization rate were not statistically different in the two groups, however, there was a significant difference in the number of embryos in the pregnant versus nonpregnant group)p< 0.05). A higher pregnancy rate and ongoing pregnancy rate occured with an endometrial thickness over 11 mm compared with below 7mm(p< 0.05, p< 0.005). however, no significant differences were noted in the implantation rate and abortion rate among the groups that classified according to their endmetrial thickness. The endometrial growth(${\Delta}$) from hCG administration day(DO) to D6 was greater in the women who achieved pregnancy than in the nonpregnant group(p< 0.01). There were no significant differences in serum estradiol levels, implantation rate, pregnancy rate, and abortion rate among the groups that classified according to the pattern of echogenesity of endometrium, however, significantly higher ongoing pregnancy rate was noted in group A, B compared with group C.(p< 0.0001, p< 0.001) These results suggest that there were no ultrasonographically detectable differences in the patterns of endometrial growth and development around the time of hCG administration in patients who conceive versus those that do not in IVF-ET.

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MBCAST: A Forecast Model for Marssonina Blotch of Apple in Korea

  • Kim, Hyo-suk;Jo, Jung-hee;Kang, Wee Soo;Do, Yun Su;Lee, Dong Hyuk;Ahn, Mun-Il;Park, Joo Hyeon;Park, Eun Woo
    • The Plant Pathology Journal
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    • v.35 no.6
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    • pp.585-597
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    • 2019
  • A disease forecast model for Marssonina blotch of apple was developed based on field observations on airborne spore catches, weather conditions, and disease incidence in 2013 and 2015. The model consisted of the airborne spore model (ASM) and the daily infection rate model (IRM). It was found that more than 80% of airborne spore catches for the experiment period was made during the spore liberation period (SLP), which is the period of days of a rain event plus the following 2 days. Of 13 rain-related weather variables, number of rainy days with rainfall ≥ 0.5 mm per day (Lday), maximum hourly rainfall (Pmax) and average daily maximum wind speed (Wavg) during a rain event were most appropriate in describing variations in airborne spore catches during SLP (Si) in 2013. The ASM, Ŝi = 30.280+5.860×Lday×Pmax-2.123×Lday×Pmax×Wavg was statistically significant and capable of predicting the amount of airborne spore catches during SLP in 2015. Assuming that airborne conidia liberated during SLP cause leaf infections resulting in symptom appearance after 21 days of incubation period, there was highly significant correlation between the estimated amount of airborne spore catches (Ŝi) and the daily infection rate (Ri). The IRM, ${\hat{R}}_i$ = 0.039+0.041×Ŝi, was statistically significant but was not able to predict the daily infection rate in 2015. No weather variables showed statistical significance in explaining variations of the daily infection rate in 2013.

Impact of After-School Education Programs on the Perceived Social Support, Self-Esteem and Behavioral Problems of Children from Broken Families (방과 후 교육 프로그램이 결손가정아동의 지각된 사회적지지, 자아존중감, 행동문제에 미치는 영향)

  • Han, Jin-Sook;Moon, Young-Sook
    • Korean Parent-Child Health Journal
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    • v.10 no.2
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    • pp.123-135
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    • 2007
  • Purpose: This study was to examine whether there were any differences in perceived social support, self-esteem and behavioral problems between children from broken families who participated in after-school education programs and others from broken families. Method: The subjects in this study were 45 children from dispersed families who were housed in a social-welfare agency in the city of Daejeon. Out of them, 22 children were grouped into an after-school educated group, and the others were selected as a uneducated group. SPSS program was used to analyze the collected data, and statistical data on real number and percentage were obtained. Besides, $x^2$-test and T-test were employed as well. Result: The major findings of the study were as follows: First, the perceived social support intergroup gap between the after-school educated group and uneducated group was not statistically significant. Second, the self-esteem intergroup gap was statistically significant(p<.01). Third, the delinquent behavior intergroup gap was statistically significant (p<.05). Fourth, as for correlational relationship among their perceived social support, self-esteem and behavioral problems, perceived social support has a positive correlation to self-esteem in the educated group(r=0.62, p<.01), and self-esteem was negatively correlated to internalizing problems in that group(r=-0.59, p<.01). The correlation of those factors was statistically significant. Conclusion: It has been confirmed that the after-school education program of broken familie child is very important in the healthy development of the children.

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Typing of Extended-Spectrum ${\beta}-Lactamase$ of Escherichia coli and Klebsiella pneumoniae Isolated from Rivers in Busan, Korea (부산지역 하천에서 분리된 장내세균 Escherichia coli와 Klebsiella pneumoniae의 광범위 베타 락탐 분해효소 (Extended-Spectrum ${\beta}-Lactamase$)에 대한 유형별 분류)

  • Lee, Hun-Ku;Kim, Hye-Jin;Kim, Gun-Do
    • Korean Journal of Microbiology
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    • v.43 no.2
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    • pp.116-123
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    • 2007
  • The purpose of this study was typing the plasmid mediated extended spectrum ${\beta}-lactamases$ produced by enteric bacteria isolated from rivers in Pusan. Six strains of Eschericha coli and fifteen strains of Klebsiella pneumoniae transferred their plasmid mediated extended spectrum ${\beta}-lactamase$ genes to the recipient strain Eschericha coli J53 $Azid^{R}$. The plasmid mediated extended spectrum ${\beta}-lactamase$ genes were sequenced directly after PCR and the types were determined by the BCM Search Launcher and GenBank nucleotid database. Determined types of the plasmid mediated extended spectrum ${\beta}-lactamases$ were TEM-52 and SHV-12. TEM-52 was isolated from both Escherichia coli and Klebsiella pneumoniae. However SHV-12 was isolated from Klebsiella pneumoniae only. The results indicated that the plasmid mediated extended spectrum ${\beta}-lactamase$ producing bacteria spreded over the area of clinical to the nature in Korea.

Validation and Application of a Real-time PCR Protocol for the Specific Detection and Quantification of Clavibacter michiganensis subsp. sepedonicus in Potato

  • Cho, Min Seok;Park, Duck Hwan;Namgung, Min;Ahn, Tae-Young;Park, Dong Suk
    • The Plant Pathology Journal
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    • v.31 no.2
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    • pp.123-131
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    • 2015
  • Clavibacter michiganensis subsp. sepedonicus (Cms) multiplies very rapidly, passing through the vascular strands and into the stems and petioles of a diseased potato. Therefore, the rapid and specific detection of this pathogen is highly important for the effective control of the pathogen. Although several PCR assays have been developed for detection, they cannot afford specific detection of Cms. Therefore, in this study, a computational genome analysis was performed to compare the sequenced genomes of the C. michiganensis subspecies and to identify an appropriate gene for the development of a subspecies-specific PCR primer set (Cms89F/R). The specificity of the primer set based on the putative phage-related protein was evaluated using genomic DNA from seven isolates of Cms and 27 other reference strains. The Cms89F/R primer set was more specific and sensitive than the existing assays in detecting Cms in in vitro using Cms cells and its genomic DNA. This assay was also able to detect at least $1.47{\times}10^2copies/{\mu}l$ of cloned-amplified target DNA, 5 fg of DNA using genomic DNA or $10^{-6}$ dilution point of 0.12 at $OD_{600}$ units of cells per reaction using a calibrated cell suspension.

Sequence and phylogenetic analysis of the phnS gene encoding 2-hydroxychromene-2-carboxylate isomerase in Sphingomonas chungbukensis DJ77 (Sphingomonas chungbukensis DJ77 균주에서 2- hydroxychromene-2-carboxylate isomerase를 암호화하는 phnS 유전자의 염기서열과 상동성 분석)

  • 엄현주;강민희;김영필;김성재;김영창
    • Korean Journal of Microbiology
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    • v.39 no.3
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    • pp.123-127
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    • 2003
  • Sphingomonas chungbukensis DJ77 is able to metabolize phenanthrene as the sole carbon and energy source. The plasmid pUPX5 includes phnS gene encoding 2-hydroxychromene-2-carboxylate (HCCA) isomerase, which is needed for phenanthrene and naphthanene degradation. We determined the nucleotide sequence of DNA fragment of 3271 bp which included the phnS gene. The fragment included an open reading frame of 594 bp which has ATG initiation codon and TAA termination codon and GGAA ribosomal binding site. The predicted amino acid sequence of the enzyme consists of 198 amino acids. The deduced amino acid sequence of the phnS enzyme exhibited 94% identity with that of the corresponding enzyme in Sphingomonas aromaticivorans F199. The phnS gene is located downstream and in the same operon as phnQ and phnR, encoding a 2,3-dihydroxybiphenyl 1,2-dioxygenase and a ferredoxin component of biphenyl dioxygenase, respectively.

Preliminary Study to Develop the Korean Medical Pathologic Aging Scale and Korean Medical Pattern Identification for Dementia (한의학 병리적 노화 척도와 치매 한의학적 변증진단 개발 및 신뢰도 평가)

  • Lee, Go eun;Moon, Kwang Su;Kim, Nam Kwen;Chung, sun yong;Jung, In Chul;Kang, Hyung Won
    • The Journal of Korean Medicine
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    • v.38 no.3
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    • pp.111-123
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    • 2017
  • Objectives: To develop and investigate the reliability of the pathologic aging scale based on korean medical theory and korean medical pattern identification for dementia. Methods: We searched the textbook of korean neurophychiatry and Donguibogam and selected items through professional consensus. We compared between dementia(n=40) and normal elderly(n=38) and tested the reliability of two scales. Results: After professional consensus, we drafted the Korean Medical Pathologic Aging Scale(12 items, Likert 3 scale) and Korean Medical Pattern Identification for Dementia(4 patterns, 28 items, Likert 5 scale). On Korean Medical Pathologic Aging Scale, There is no significant difference between two groups. We had good internal consistency(Cronbach's alpha = 0.6) and test-retest reliability(r=0.631) but low inter-rater reliability(r=0.430). On Korean Medical Pattern Identification for Dementia, dementia patients diagnosed with Qi deficiency are significantly more than those in normal group. We had fairly good internal consistency(Cronbach's alpha = 0.574) and excellent test-retest(kappa= .800) and inter-rater reliability(kappa = .733). Conclusions: Korean Medical Pattern Identification for Dementia is appropriate for diagnosing korean medical pattern. But Korean Medical Pathologic Aging Scale isn't appropriate to discriminate dementia from normal elderly because of many subjective items. Therefore objective measurement of sensory dysfunction would be needed to measure pathologic aging based on korean medical theory.

Effects of a Au-Cu Back Layer on the Properties of Spin Valves

  • In, Jang-Sik;Kim, Sang-Hoon;Kang, Jae-Yong;Tiwari, Ajay;Hong, Jong-Ill
    • Journal of Magnetics
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    • v.12 no.3
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    • pp.118-123
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    • 2007
  • We have studied the effect of Au-Cu back layer system ${\sim}10{\AA}$ thick on the properties of a spin valve. The back layers were Cu, Au, co-sputtered $Cu_xAu_{1-x}$, laminated $[Au/Cu]_n$. and bi-layer [Au/Cu]. When Au was added to the Cu, the resistance of the spin valve abruptly increased most likely due to impurity scattering. The GMR values were not increased significantly for all the structures. In the case of co-sputtered $Cu_xAu_{1-x}$, the changes in the resistance, ${\Delta}R$, was increased at a composition of ${\sim}Au_{0.5}Cu_{0.5}$. This increase in ${\Delta}R$ is due to increase in the resistance and not from the enhanced spin-dependent scattering. The structural analyses showed that the orthorhombic $Au_{0.5}Cu_{0.5}$ was formed in the back layer instead of the face-centered tetragonal $Au_{0.5}Cu_{0.5}$ as we expected. Thermal annealing over $400^{\circ}C$ may be required to have face-centered tetragonal in the $10{\AA}$ thick ultra-thin film. In the case of a laminated or bi-layered back layer, the properties of the spin valve were improved, which may be attributed to the increase in the mean free path of conduction electrons.

The Effect of Problem Based Learning on Nursing Students' Interaction and Self-directed Learning: A Social Network Analysis (문제중심학습방법이 대학생들의 학습자 상호작용 및 자기주도학습능력에 미치는 영향: 사회연결망 분석을 중심으로)

  • Piao, Mei Hua;Kim, Jeong Eun
    • Perspectives in Nursing Science
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    • v.13 no.1
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    • pp.29-35
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    • 2016
  • Purpose: This study aimed to explore the underlying structures of students' interaction networks to monitor network changes during the year, to verify the relationship with self-directed learning, and to identify the effect of problem-based learning on interaction and self-directed learning. Methods: A longitudinal study was designed which included 3 parts (A=25, B=27, C=26) with a total of 78 second-year nursing students from 2013 to 2014. Interaction indicators used group network centralization and density, and individual in-degree centrality. Results: Group network centralization showed mean reversion patterns, however, centralization and density showed a slight increase from 2013 to 2014 (Centralization of A part from 52.78 to 36.96, B part from 20.56 to 32.20, C part from 34.40 to 37.24; Density of A part from 0.122 to 0.123, B part from 0.111 to 0.121, C part from 0.109 to 0.121). The individual in-degree centrality is significantly correlated with self-directed learning and the correlation coefficient increased during the year (r=.274 in 2013, r=.356 in 2014, p<.001). Conclusion: Students share information more interactively during the year and the more they share the higher the scores of self-directed learning.