Browse > Article

Typing of Extended-Spectrum ${\beta}-Lactamase$ of Escherichia coli and Klebsiella pneumoniae Isolated from Rivers in Busan, Korea  

Lee, Hun-Ku (Department of Microbiology, Pukyong National Univ.)
Kim, Hye-Jin (Department of Microbiology, Pukyong National Univ.)
Kim, Gun-Do (Department of Microbiology, Pukyong National Univ.)
Publication Information
Korean Journal of Microbiology / v.43, no.2, 2007 , pp. 116-123 More about this Journal
Abstract
The purpose of this study was typing the plasmid mediated extended spectrum ${\beta}-lactamases$ produced by enteric bacteria isolated from rivers in Pusan. Six strains of Eschericha coli and fifteen strains of Klebsiella pneumoniae transferred their plasmid mediated extended spectrum ${\beta}-lactamase$ genes to the recipient strain Eschericha coli J53 $Azid^{R}$. The plasmid mediated extended spectrum ${\beta}-lactamase$ genes were sequenced directly after PCR and the types were determined by the BCM Search Launcher and GenBank nucleotid database. Determined types of the plasmid mediated extended spectrum ${\beta}-lactamases$ were TEM-52 and SHV-12. TEM-52 was isolated from both Escherichia coli and Klebsiella pneumoniae. However SHV-12 was isolated from Klebsiella pneumoniae only. The results indicated that the plasmid mediated extended spectrum ${\beta}-lactamase$ producing bacteria spreded over the area of clinical to the nature in Korea.
Keywords
Escherichia coli; extended-spectrum ${\beta}-lactamase$ (ESBL); isoelectric focusing (IEF); Klebsiella; pnuemoniae; SHV-12; TEM-52;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By SCOPUS : 2
연도 인용수 순위
1 이훈구. 2001. 광안리 오수처리장에서 분리된 extendedspectrum $beta$-lactamase (ESBL) Klebsiella와 Enterobacter의 유형. 한국미생물학회지 37, 277-283
2 정윤섭, 이경원, 오까모도료이찌, 이노우에 마쓰히사. 1997. 임상검체에서 분리된 extended-spectrum $beta$-lactam 항균제 분해 Klebsiella pneumoniae와 Escherichia coli의 성상. 감염 29, 477-485
3 Goussard, S. and P. Courvalin. 1999. Update sequence information for TEM $beta$-lactamase gene. Antimicrob. Agents Chemother. 43, 367-370   DOI   PUBMED   ScienceOn
4 Nueesch-Inderbinen, M.T., F.H. Kayser, and H. Haechlier. 1997. Survey and molecular genetics of SHV $beta$-lactamases in Enterobacteriaceae in Switzerland: two novel enzymes, SHV-11 and SHV-12. Antimicrob. Agents Chemother. 41, 943-949   PUBMED
5 Pitout, J.D.D., K.S. Thomoson, N.D. Hanson, A.F. Ehrhardt, P. Cudron, and C. Sanders. 1998. Plasmid-mediated resistance to extended-spectrum cephalosporins among Enterobacter aerugenes strains. Antimicrob. Agents Chemother. 42, 596-600   PUBMED
6 Jacoby, G.A. and I. Carreras. 1990. Activities of $beta$-lactam antibiotics against Escherichia coli strains producing extended-spectrum $beta$-lactamases. Antimicrob. Agents Chemother. 34, 858-862   DOI   PUBMED   ScienceOn
7 Poyart, C., P. Mugnier, G. Quesum, P. Berche, and P. Trieu-Cout.1998. A novel extended-spectrum TEM-type $beta$-lactamase (TEM-52) associated with decreased susceptibility to moxalactam in Klebsiella pneumoniae. Antimicrob. Agents Chemother. 42, 108-113   PUBMED
8 이훈구. 2006. 부산 도축장에서 분리된 광범위 베타 락 탐 분해효소(extended-spectrum $beta$-lactamase, ESBL) 생성 장내세균의 형별분류. 한국미생물학회지 42, 125-130   과학기술학회마을
9 Pai, H.J., S. Lyu, J.H. Lee, J.M. Kim, Y.M. Kwon, J.W. Kim, and K.W. Choe. 1999. Survey of extended-spectrum $beta$-lactamases in clinical isolates of Escherichia coli and Klesbsiella pneumoniae : prevalence of TEM-52 in Korea. J. Clin. Microbiol. 37, 1758-1763   PUBMED
10 Philippon, A., R. Labia, and G. Jacobi. 1989. Extended-spectrum $beta$-lactamases (minireview). Antimicrob. Agents Chemother. 33, 1131-113   DOI   PUBMED   ScienceOn
11 Rasheed, J.K., C. Jay, B. Metchock, F. Berkowitz, L. Wiegel, J.Crellin, C. Steward, B. Hill, A.A. Meideiros and F.C. Tenover. 1997. Evoution of extended spectrum $beta$-lactam resistance (SHV-8) in a strain of Escherichia coli during multiple episodes of bacteremia. Antimicrob. Agents Chemother. 41, 647-653   PUBMED
12 Ambuler, R.P., A.F.W. Coulson, N.M. Frere, J.M. Ghuysen, B. Joris, M. Forsman, R.C. Lebversque, G. Tiraby, and S.G. Waley. 1991. A standard numbering scheme for class A $beta$-lactamases. Biochem. J. 276, 269-272   DOI   PUBMED
13 National Committee for Clinical Laboratory Standards. 1998. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. 4th ed. Pennsylvania: NCCLS: M7-A4
14 이경원, 조성란, 이창숙, 정윤섭, 권오현. 1994. Extended broad-spectrum $beta$-lactamase 생성 Escherichia coli와 Klebsiella pneumoniae. 감염 26, 341-348
15 Bush, K., G.A. Jacoby, and A.A. Medeiros. 1995 A functional classification scheme for $beta$-lactamases and its correlation with molecular structure. Antimicrob. Agents Chemother. 39, 1211-1233   DOI   PUBMED   ScienceOn
16 Meideiros, A.A. 1997. Evolution and dissemination of $beta$-lactamases accelerated by generation of $beta$-lactam antibiotics. Clin. Infect. Dis. 245, 19-45
17 Miranda, G., N. Castro, and B. Leanos. 2004. Clonal and horizontal dissemination of Klebsiella pneumoniae expressing SHV-5 extended-spectum $beta$-lactamase in a Mexican pediatric hospital. J. Clin. Microbial. 42, 30-35   DOI
18 Matthew, M., R.W. Hedges, and J.T. Smith. 1979. Types of $beta$-lactamase determined by plasmid in Gram-negative bacteria. J. Bacteriol. 138, 657-662   PUBMED
19 Teshager, T., L. Dominguez, and M.A. Moreno. 2000. Isolaton of an SHV-12 $beta$-lactamase producing Escherichia coli strain from a dog with recurrent urinary tract infections. Antimicrob. Agents Chemother. 44, 3483-3484   DOI
20 이선화, 김미나, 최수진, 정화순. 2000. 임상 검체에서 분리된 Escherichia coli의 extended-spectrum $beta$-lactamase 성상. 대한임상병리학회지 20, 400-409
21 Bagattini, M., V. Crivaro, A.D. Popolo, F. Gentile, A. Scarcella, M. Triassi, P. Villari, and R. Zarilli. 2006. Molecular epidemiology of extended-spectrum (beta)-lactamase producing Klebsiella pneumonia in a neonatal intensive care unit. J. Antimicrobe. Chemother. 57, 979-982   DOI   ScienceOn
22 Sambrook, J., F.E. Fritsch, and T. Manilatis. 1989. Molecular cloning laboratory manual. Cold Spring Harbor Laboratory Press. I. 1.25-1.28
23 Jeong, Y.S., J.C. Lee, H.Y. Kang, H.S. Yu, E.Y. Lee, C.H. Choi, S.H. Tae, Y.C. Lee, D.T. Cho, and S.Y. Sul. 2003. Epidemiology of nalidixic acid resistance and TEM-1- and TEM-52-mediated ampicillin resistance of Shigella sonnei isolated obtained in Korea between 1980 and 2000. Antimicrob. Agents Chemother. 47, 3719-3723   DOI
24 Jacoby, G.A. and P. Han. 1996. Detection of extended-spectrum $beta$-lactamases in clinical isolates of Klebsiella pneumoniae and Escherichia coli. J. Clin. Microbiol. 34, 908-911   PUBMED
25 Billot-Klein, D., L. Gutmann, and E. Collatz. 1990. Nucleotide sequence of the SHV-5 $beta$-lactamase gene of a Klebsiella pneumoniae plasmid. Antimicrob. Agents Chemother. 34, 2439-2441   DOI   PUBMED   ScienceOn
26 Brinas, L., M. Zarazaga, Y. Saenz, F. Ruiz-Larrea, and C. Torres. 2002. $beta$- lactamases in ampicillin-resistant Escherichia coli isolates from foods, humans, and healthy animals. Antimicrob. Agents Chemother. 46, 3156-3163   DOI
27 Bush, K. 1989. Characterization of $beta$-lactamases. Antimicrob. Agents Chemother. 33, 259-263   DOI   PUBMED   ScienceOn
28 Poirel, L., T. Naas, M. Guibert, E.B. Chaibi, R. Labia, and P. Nordmann. 1999. Molecular and Biochemical characterization of VEB-1 a novel class a extended spectrum $beta$-lactamase encoded by an Escherichia coli integron gene. Antimorob. Agents Chemother. 43, 573-581
29 Karen, B. and J. George. 1997. Nomenclature of TEM $beta$‚lactamase. Antimicrob. Agents Chemother. 39, 1-3
30 Winokur, P.L., A. Brueggemann, D.L. Desalvo, L. Hoffmann, M.D. A.E.K. Uhlenhopp, M.A. Pfaller, and G.V. Doern. 2000. Animal and human multidrug-resistant, cephalosporin-resistant Salmonella isolates expressing a plasmid-mediated CMY-2 AmpC $beta$-lactamase. Antimicrob. Agents Chemother. 44, 2777-2783   DOI
31 Ewing, W.H. 1986. Identification of Enterobacteriaceae. Elsevier, N.Y., USA
32 Bush, K. and G. Jacoby. 1997 Nomenclature of TEM $beta$-lactamases. J. Antimicrob. Chemother. 39, 1-3