• 한국응용곤충학회:학술대회논문집
• /
• 한국응용곤충학회 2003년도 추계 학술발표회
• /
• pp.112-112
• /
• 2003

• 한국잠사학회:학술대회논문집
• /
• 한국잠사학회 2003년도 제46회 춘계 학술연구 발표회
• /
• pp.44-44
• /
• 2003

Protein disulfide isomerase (PDI) is not only an isomerase catalyzing the formation of native disulfide bond(s) of nascent peptide, but also a molecular chaperone assisting chain folding. We have already reported the structure of a cDNA (bPDl) encoding PDI from Bombyx mori and the function of PDI as foldase in assisting protein folding. (omitted)

• 한국약용작물학회:학술대회논문집
• /
• 한국약용작물학회 2008년도 추계학술발표회
• /
• pp.133-134
• /
• 2008

• 한국분말야금학회:학술대회논문집
• /
• 한국분말야금학회 2006년도 Extended Abstracts of 2006 POWDER METALLURGY World Congress Part 1
• /
• pp.311-311
• /
• 2006

• 한국잠사학회:학술대회논문집
• /
• 한국잠사학회 2003년도 제46회 춘계 학술연구 발표회
• /
• pp.45-45
• /
• 2003

Protein disulfide isomerase (PDO) is an essential protein which is localized to the endoplasmic reticulum (ER) of eukaryotic cells. It catalyses the formation and isomerization of disulfide bonds during the folding of secretory proteins. We have isolarted a cDNA encoding PDI from Bombyx mori (bPDI), in which an open reading frame of 494 mino acid (55.6kDa) is shown. (omitted)

• 한국경영과학회지
• /
• 제40권4호
• /
• pp.19-33
• /
• 2015

Recently, interest in research and development (R&D) investment decisions have increased among Korean domestic enterprises. However, existing R&D investment studies only focused on government R&D investment policies while only a few studies investigated firm level R&D investment. Prior literatures also overlooked the feedback loop between R&D investment and firm performance. Therefore, this paper identifies a system dynamics model for R&D investment decision making in domestic electronics firms. The conceptual model is derived from R&D investment-related theories found in bodies of literature on company performance, enterprise activity, and market maturity. This study investigates the dynamic feedback between R&D activities and sales using the system dynamics model. In other words, the system dynamics model is used to explain the change in the closed feedback circulation structure in R&D investment activities including technology development, production process, and marketing that subsequently result in sales increase and re-investment into R&D from the generated revenues. There are two major results. First, a similar ratio of investment on technology development and production process derives the higher company sales. Second, regardless of market maturity, marketing investment ratio positively affects sales and R&D budget growth. This study provides a system dynamics model to find the optimal ratio for R&D investment and suggests managerial strategic implications on electronic firm R&D investment decision making under market maturity condition.

• 생명과학회지
• /
• 제24권7호
• /
• pp.798-803
• /
• 2014

Cathepsin D (CtsD)는 아스파르트산 단백질 분해효소로서 cytochrome C의 방출을 유도하여 apoptosis 기전을 활성화시킨다. 본 연구에서는 3T3-L1 지방전구세포에서 CtsD 발현 조절에 관여하는 microRNA에 대해 조사하였다. 먼저 지방전구세포 사멸시 CtsD 발현 변화를 관찰하기 위하여 DNA damage agent인 doxorubicin을 3T3-L1 세포주에 노출시켜 CtsD 발현이 증가함을 확인하였다. 또한 지방전구세포주에서 CtsD가 과발현되면 세포 생존율이 감소하였다. miRanda program을 이용하여 CtsD 유전자를 표적으로 하는 microRNA를 탐색하여 miR-145를 선발하였다. Luciferase reporter assay에 의해 miR-145가 CtsD 유전자의 3' UTR 부위에 결합하여 luciferase 활성을 감소시킴을 관찰하였다. 3T3-L1 세포주에 miR-145 mimic을 도입한 결과 CtsD mRNA 발현과 단백질 수준이 감소하였다. 또한 세포주에 doxorubicin을 처리한 결과 CtsD 유전자 발현 증가와 상반되게 miR-145 발현이 감소하였다. 이외에도 miR-145 inhibitor을 세포에 도입하면 세포 생존율이 감소하였다. 이러한 결과는 지방전구세포의 세포사멸에 CtsD가 관여할 수 있으며, miR-145에 의해 CtsD 발현이 직접 조절되고 있음을 나타낸다. 따라서, 지방전구세포의 사멸을 유도하기 위해서는 miR-145 발현 제어가 주요한 표적이 될 수 있을 것으로 생각된다. 본 연구결과는 향후 비만 예방 및 치료를 위한 지방세포 사멸기전 규명에 중요한 기초 자료를 제공할 수 있을 것으로 기대한다.

• Biomolecules & Therapeutics
• /
• 제27권6호
• /
• pp.514-521
• /
• 2019

G protein-coupled receptors (GPCRs) are membrane receptors whose agonist-induced dynamic conformational changes trigger heterotrimeric G protein activation, followed by GRK-mediated phosphorylation and arrestin-mediated desensitization. Cytosolic regions of GPCRs have been studied extensively because they are direct contact sites with G proteins, GRKs, and arrestins. Among various cytosolic regions, the role of helix 8 is least understood, although a few studies have suggested that it is involved in G protein activation, receptor localization, and/or internalization. In the present study, we investigated the role of helix 8 in dopamine receptor signaling focusing on dopamine D1 receptor (D1R) and dopamine D2 receptor (D2R). D1R couples exclusively to Gs, whereas D2R couples exclusively to Gi. Bioinformatic analysis implied that the sequences of helix 8 may affect GPCR-G protein coupling selectivity; therefore, we evaluated if swapping helix 8 between D1R and D2R changed G protein selectivity. Our results suggest that helix 8 is not involved in D1R-Gs or D2R-Gi coupling selectivity. Instead, we observed that D1R with D2R helix 8 or D1R with an increased number of hydrophobic residues in helix 8 relative to wild-type showed diminished ${\beta}$-arrestin-mediated desensitization, resulting in increased Gs signaling.

• 대한산업공학회지
• /
• 제43권2호
• /
• pp.100-111
• /
• 2017

Evaluating the performance of R&D Activity is complicated because it is hard to quantify the R&D results unlike the traditional manufacturing or service industries. Recently, to overcome this, process-focused evaluation methods applying the philosophy of quality into R&D environment have been introduced. However, these quality activities are mainly conducted without feedback system after the evaluation work is done. The aim of this study is to present a R&D quality diagnosis framework to obtain the improvement opportunities from R&D process perspective. The research is designed as follows : First, R&D standard process and R&D quality elements are derived from a literature review. Second, the diagnosis objects are obtained by investigating the R&D quality elements at each R&D steps. Third, a two-dimensional diagnosis model, which enables the objective measurement of the 'system compatibility' and 'accomplish level', is presented. The proposed method can provide an effective way to find opportunities for efficient quality improvement of R&D process.

• 한국경영과학회:학술대회논문집
• /
• 한국경영과학회 2007년도 추계학술대회 및 정기총회
• /
• pp.141-144
• /
• 2007

This research aims to propose model for measuring the efficiency of R&D organizations by using Data Envelopment Analysis. In existing research about using Data Envelopment Analysis, the types of R&D organizations have not been clarified and the difference between analysis time from measurement time is usually $4{\sim}5$ years. This research divides R&D organizations into three types and provides proper methods for measuring the efficiency of each R&D organizations.