• Food Science of Animal Resources
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• v.26 no.3
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• pp.402-409
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• 2006

Although microorganisms are, in fact, the most diverse and abundant type of organism on Earth, the ecological functions of microbial populations remains poorly understood. A variety of bacteria including marine Vibrios encounter numerous ecological challenges, such as UV light, predation, competition, and seasonal variations in seawater including pH, salinity, nutrient levels, temperature and so forth. In order to survive and proliferate under variable conditions, they have to develop elaborate means of communication to meet the challenges to which they are exposed. In bacteria, a range of biological functions have recently been found to be regulated by a population density-dependent cell-cell signaling mechanism known as quorum-sensing (QS). In other words, bacterial cells sense population density by monitoring the presence of self-produced extracellular autoinducers (AI). N-acylhomoserine lactone (AHL)-dependent quorum-sensing was first discovered in two luminescent marine bacteria, Vibrio fischeri and Vibrio harveyi. The LuxI/R system of V. fischeriis the paradigm of Gram-negative quorum-sensing systems. At high population density, the accumulated signalstrigger the expression of target genes and thereby initiate a new set of biological activities. Several QS systems have been identified so far. Among them, an AHL-dependent QS system has been found to control biofilm formation in several bacterial species, including Pseudomonas aeruginosa, Aeromonas hydrophila, Burkholderia cepacia, and Serratia liquefaciens. Bacterial biofilm is a structured community of bacterial cells enclosed in a self-produced polymeric matrix that adheres to an inert or living surface. Extracellular signal molecules have been implicated in biofilm formation. Agrobacterium tumefaciens strain NT1(traR, tra::lacZ749) and Chromobacterium violaceum strain CV026 are used as biosensors to detect AHL signals. Quorum sensing in lactic acid bacteria involves peptides that are directly sensed by membrane-located histidine kinases, after which the signal is transmitted to an intracellular regulator. In the nisin autoregulation process in Lactococcus lactis, the NisK protein acts as the sensor for nisin, and NisR protein as the response regulator activatingthe transcription of target genes. For control over growth and survival in bacterial communities, various strategies need to be developed by which receptors of the signal molecules are interfered with or the synthesis and release of the molecules is controlled. However, much is still unknown about the metabolic processes involved in such signal transduction and whether or not various foods and food ingredients may affect communication between spoilage or pathogenic bacteria. In five to ten years, we will be able to discover new signal molecules, some of which may have applications in food preservation to inhibit the growth of pathogens on foods.

• Journal of KIISE:Information Networking
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• v.33 no.3
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• pp.193-200
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• 2006

The security feature is essential in wireless sensor network such as intrusion detection or obstacle observation. Sensor nodes must have shared secret between nodes to support security such as privacy. Many methods which provide key pre-distribution need too many keys or support poor security. To solve this problem, probabilistic key pre-distribution is proposed. This method needs a few keys and use probabilistic method to share keys. However, this method does not guarantee key sharing between nodes, and neighbor nodes nay not communicate each other. It leads to waste of network resource such as inefficient routing, extra routing protocol. In this paper, we propose new key distribution method using quorum system which needs a few keys and guarantee key sharing between nodes. We also propose extension of the method which needs fewer keys and guarantee key sharing when node deployment knowledge is well known.

• Environmental Engineering Research
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• v.24 no.4
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• pp.543-558
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• 2019

In comparison to alternative advanced wastewater treatment technologies, the main problem associated with membrane bioreactor (MBR) technology, which has become prominent in recent years, is biofouling. Within these systems, biofouling is typically the result of a biofilm layer resulting from bacterial gathering. One biological system that can be employed to interrupt the process of bacterial gathering is called 'Quorum Quenching (QQ)'. Existing QQ applications can be classified using three main types: 1) bacterial/whole-cell applications, 2) direct enzyme applications, and 3) natural sourced compounds. The most common and widely recognized applications for membrane fouling control during MBR operation are bacterial and direct enzyme applications. The purpose of this review was to identify and assess biofilm formation mechanism and results, the suggestion of the QQ concept and its potential to control biofilm formation, and the means by which these QQ applications can be applied within the MBR and present QQ MBR studies.

• Journal of Life Science
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• v.26 no.12
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• pp.1487-1497
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• 2016

Bacterial cell to cell communication strategies called quorum sensing (QS) using small diffusible signaling molecules (auto-inducers) govern the expression of various genes dependent on their population density manner. As a consequence of synthesis and response to the signaling molecules, individual planktonic cells synchronized group behaviors to control a diverse array of phenotypes such as maturation of biofilm, production of extra-polymeric substances (EPS), virulence, bioluminescence and antibiotic production. Many studies indicated that biofilm formations are associated with QS signaling molecules such as acyl-homoserine lactones (AHLs) mainly used by several Gram negative bacteria. The biofilm maturation causes undesirable biomass accumulation in various surface environments anywhere water is present called biofouling, which results in serious eco-technological problems. Numerous molecules that interfere the bacterial QS called quorum quenching (QQ), have been discovered from various microorganisms, and their functions and mechanisms associated with QS have also been elucidated. To resolve biofouling problems related to various industries, the novel approach based on QS interference has been emerged attenuating multi-drug resisting bacteria appearance and environmental toxicities, which may provide potential advantages over the conventional anti-biofouling approaches. Therefore this paper presents recent information related to bacterial quorum sensing system, quorum quenching enzymes that can control the QS signaling, and lastly discuss the anti-biofouling approaches using the quorum quenching.

• Journal of Microbiology and Biotechnology
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• v.26 no.12
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• pp.2159-2170
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• 2016

Many plant-pathogenic bacteria are dependent on quorum sensing (QS) to evoke disease. In this study, the population of QS and quorum quenching (QQ) bacteria was analyzed in a consecutive monoculture system of Pseudostellaria heterophylla. The isolated QS strains were identified as Serratia marcescens with SwrIR-type QS system and exhibited a significant increase over the years of monoculture. Only one QQ strain was isolated from newly planted soil sample and was identified as Bacillus thuringiensis, which secreted lactonase to degrade QS signal molecules. Inoculation of S. marcescens to P. heterophylla root could rapidly cause wilt disease, which was alleviated by B. thuringiensis. Furthermore, the expression of lactonase encoded by the aiiA gene in S. marcescens resulted in reduction of its pathogenicity, implying that the toxic effect of S. marcescens on the seedlings was QS-regulated. Meanwhile, excess lactonase in S. marcescens led to reduction in antibacterial substances, exoenzymes, and swarming motility, which might contribute to pathogensis on the seedlings. Root exudates and root tuber extracts of P. heterophylla significantly promoted the growth of S. marcescens, whereas a slight increase of B. thuringiensis was observed in both samples. These results demonstrated that QS-regulated behaviors in S. marcescens mediated by root exudates played an important role in replanting diseases of P. heterophylla.

• Journal of Korea Multimedia Society
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• v.24 no.8
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• pp.1101-1113
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• 2021

The rapid growth of sense-and-respond applications and the emerging cloud computing model present a new challenge: providing publish/subscribe middleware as a scalable and elastic cloud service. The publish/subscribe interaction model is a promising solution for scalable data dissemination over wide-area networks. In addition, there have been some work on the publish/subscribe messaging paradigm that guarantees reliability and availability in the face of node and link failures. These publish/subscribe systems are commonly used in information-centric networks and edge-fog-cloud infrastructures for IoT. The IoT has an edge-fog cloud infrastructure to efficiently process massive amounts of sensing data collected from the surrounding environment. In this paper. we propose a quorum-based hierarchical fault-tolerant publish/subscribe systems (QHFPS) to enable reliable delivery of messages in the presence of link and node failures. The QHFPS efficiently distributes IoT messages to the publish/subscribe brokers in fog overlay layers on the basis of proposing extended stepped grid (xS-grid) quorum for providing tolerance when faced with node failures and network partitions. We evaluate the performance of QHFPS in three aspects: number of transmitted Pub/Sub messages, average subscription delay, and subscritpion delivery rate with an analytical model.

• Journal of Korea Multimedia Society
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• v.25 no.8
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• pp.1075-1084
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• 2022

Internet of Things(IoT) has become a key available technology for efficiently implementing device to device(D2D) services in various domains such as smart home, healthcare, smart city, agriculture, energy, logistics, and transportation. A lightweight publish/subscribe(Pub/Sub) messaging protocol not only establishes data dissemination pattern but also supports connectivity between IoT devices and their applications. Also, a Pub/Sub broker is deployed to facilitate data exchange among IoT devices. A scalable edge-based publish/subscribe (Pub/Sub) broker overlay networks support latency-sensitive IoT applications. In this paper, we design a hypercube grid quorum(HGQ) for distributed Pub/Sub systems based IoT applications. In designing HGQ, the network of hypercube structures suitable for the publish/subscribe model is built in the edge layer, and the proposed HGQ is designed by embedding a mesh overlay network in the hypercube. As their applications, we propose an HGQ-based mechansim for dissemination of the data of sensors or the message/event of IoT devices in IoT environments. The performance of HGQ is evaluated by analytical models. As the results, the latency and load balancing of applications based on the distributed Pub/Sub system using HGQ are improved.

• The Plant Pathology Journal
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• v.27 no.3
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• pp.242-248
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• 2011

Several soil bacteria were found to degrade N-Acylhomoserine lactones (NAHLs), thereby interfering with the bacterial quorum sensing system. In this research, fifteen strains of NAHL degrading rhizobacteria were isolated from potato rhizosphere. Based on phenotypic characteristics and 16S rDNA sequence analyses, the strains were identified as members of genera Bacillus, Streptomyces, Arthrobacter, Pseudomonas and Mesorhizobium. All tested isolates were capable to degrade both synthetic and natural NAHL produced by Pectobacterium carotovorum subsp. carotovorum (Pcc) strain EMPCC. In quorum quenching experiments selected isolates, especially Mesorhizobium sp., were markedly reduced the pathogenicity of Pcc strain EMPCC in potato tubers and totally suppressed tissue maceration on potato tubers. These led to consider the latter as a useful biocontrol agent against Pectobacterium spp.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.224.1-224.1
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• 2003

Recent studies have revealed that bacterial biofilm production by the gram-negative bacteria is regulated by the quorum sensing signaling molecules, AHLs (N-acyl homoserine lactones). This suggests that inhibiting the AHLs could enhance the effects of antibacterial agents. Halogenated furanones purified from the red algae Delisea pulchra have been known to decrease quorum sensing responses by competitive inhibition of the AHLs. (omitted)

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.8 no.2
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• pp.9-14
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• 2008

Mobile Ad-Hoc network is the network of mobile nodes which has no fixed infrastructure, and mobile node in MANET can move freely and communicate with each other. The location management is an important issue because location information of a mobile node is frequently changed in MANET. In this paper, we propose new quorum system applying the gravity of locality of mobile nodes adaptively by using the fuzzy-logic for the mobility of mobile nodes in order to manage location information of mobile nodes in MANET efficiently. The performance of the proposed scheme is evaluated by an analytical model and compared with those of existing UQS and DQS schemes.