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http://dx.doi.org/10.5423/PPJ.2011.27.3.242

Virulence Attenuation of Pectobacterium carotovorum Using N-Acyl-homoserine Lactone Degrading Bacteria Isolated from Potato Rhizosphere  

Mahmoudi, Esmaeil (Department of Plant Protection, Khorasgan Branch, Islamic Azad University)
Tabatabaei, Badraldin Ebrahim Sayed (Department of Agricultural Biotechnology, Isfahan University of Technology)
Venturi, Vittorio (Bacteriology Group, International Center for Genetic Engineering and Biotechnology)
Publication Information
The Plant Pathology Journal / v.27, no.3, 2011 , pp. 242-248 More about this Journal
Abstract
Several soil bacteria were found to degrade N-Acylhomoserine lactones (NAHLs), thereby interfering with the bacterial quorum sensing system. In this research, fifteen strains of NAHL degrading rhizobacteria were isolated from potato rhizosphere. Based on phenotypic characteristics and 16S rDNA sequence analyses, the strains were identified as members of genera Bacillus, Streptomyces, Arthrobacter, Pseudomonas and Mesorhizobium. All tested isolates were capable to degrade both synthetic and natural NAHL produced by Pectobacterium carotovorum subsp. carotovorum (Pcc) strain EMPCC. In quorum quenching experiments selected isolates, especially Mesorhizobium sp., were markedly reduced the pathogenicity of Pcc strain EMPCC in potato tubers and totally suppressed tissue maceration on potato tubers. These led to consider the latter as a useful biocontrol agent against Pectobacterium spp.
Keywords
Mesorhizobium; NAHLs; Pectobacterium carotovorum; Quorum sensing;
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