• 한국산학기술학회논문지
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• 제17권12호
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• pp.427-436
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• 2016

도심지에 지진, 산사태 등과 같은 재난 발생 시 건물 및 지하 구조물 붕괴로 인해 잔해 내부에 다수의 매몰자가 발생된다. 이때 인명탐지를 위해 주로 음향, 영상 및 전파 등을 활용한 탐지 장비 등이 활용되나 고가이며, 붕괴지 상부로의 직접 투입으로 인한 2차 붕괴위험 및 장비 운용 성능 저하로 인해 신속하고 높은 신뢰성을 갖는 인명탐지 기술이 활용되지 않고 있다. 이러한 문제를 해결하기 위한 매몰자의 휴대 기기에서 송출하는 Wi-Fi 신호 및 기압정보를 제공받아 매몰자의 3차원 위치를 탐색하는 UAV(Unmanned Aerial Vehicle)에 탑재 가능한 무선신호 기반 인명탐지 모듈을 개발하였다. 또한 드론의 비행동안 매몰자 휴대기기 정보를 실시간으로 수집하여 해당 정보를 지상부에 전송하여 신뢰성 있는 매몰자의 3차원 위치정보를 제공하도록 하는 모듈 개발 프레임워크를 제시하였다. 이를 통해 인명탐지 모듈의 개발과 현장 테스트를 통해 적용 타당성을 검증하였다. 이러한 연구결과는 향후 대형 건물 붕괴와 같은 재난 시 매몰자에 대한 매몰 위치의 신속한 탐지 및 구호와 실종자 수색을 위한 핵심기술로 활용될 수 있을 것이다.

• BMB Reports
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• 제37권5호
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• pp.546-551
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• 2004

The increasing pace of development in molecular biology during the last decade has had a direct effect on mass testing and diagnostic applications, including blood screening. We report the model Microarray that has been developed for Hepatitis B virus (HBV) and Hepatitis D virus (HDV) detection. The specific primer pairs of PCR were designed using the Primer Premier 5.00 program according to the conserved regions of HBV and HDV. PCR fragments were purified and cloned into pMD18-T vectors. The recombinant plasmids were extracted from positive clones and the target gene fragments were sequenced. The DNA microarray was prepared by robotically spotting PCR products onto the surface of glass slides. Sequences were aligned, and the results obtained showed that the products of PCR amplification were the required specific gene fragments of HBV, and HDV. Samples were labeled by Restriction Display PCR (RD-PCR). Gene chip hybridizing signals showed that the specificity and sensitivity required for HBV and HDV detection were satisfied. Using PCR amplified products to construct gene chips for the simultaneous clinical diagnosis of HBV and HDV resulted in a quick, simple, and effective method. We conclude that the DNA microarray assay system might be useful as a diagnostic technique in the clinical laboratory. Further applications of RD-PCR for the sample labeling could speed up microarray multi-virus detection.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2011년도 한국컴퓨터종합학술대회논문집 Vol.38 No.1(C)
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• pp.405-408
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• 2011

In this paper, we propose a robot remote control system based on mouth tracking. The main idea behind the work is to help disabled people who cannot operate a joystick or keyboard to control a robot with their hands. The mouth detection method in this paper is mainly based on the Adaboost feature detection approach. By using the proposed new Haar-like features for detecting the corner of mouth, the speed and accuracy of detection are improved. Combined with the Kalman filter, a continuous and accurate mouth tracking has been achieved. Meanwhile, the gripping commands of the robot manipulator were also achieved by the recognition of the user.s mouth shape, such as 'pout mouth' or 'grin mouth'. To assess the validity of the method, a mouth detection experiment and a robot cargo transport experiment were applied. The result indicated that the system can realize a quick and accurate mouse tracking; and the operation of the robot worked successfully in moving and bringing back items.

• 방송공학회논문지
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• 제21권2호
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• pp.268-271
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• 2016

배경 모델과 배경 차분화로 구성되어 있는 전경객체 추출은 다양한 컴퓨터 비젼 응용에서 중요한 기능이다. 조명 변화를 고려하지 않은 기존 방법들은 급격한 조명 변화에서는 성능이 저하된다. 본 레터에서는 이 문제를 해결할 수 있는 조명 변화에 강인한 배경 모델링 방법을 제안한다. 제안 방법은 다른 적응률을 가진 두 개의 배경 모델을 사용함으로써 조명 조건에 신속하게 적응할 수 있다. 본 논문의 제안 방법은 non-parametric 기법으로서 실험에서는 기존 non-parametric 기법들보다 우수한 성능 및 낮은 복잡도를 보여줌을 증명하였다.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제1권1호
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• pp.83-89
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• 2020

Methods for detecting the presence of genetically modified (GM) crops are evolving to comply with legislation and to enhance monitoring by biotechnology companies and regulators. In order to cover a broad range of detection methods for a new GM crop, conventional multiplex PCR methods are required. Based on the genetic information on three GM alfalfa varieties (J101, J163, and KK179), which were recently approved in South Korea, we developed a fast, reliable, and highly specific multiplex polymerase chain reaction (PCR) method with basic PCR equipment and inexpensive reagents. To validate and verify the newly developed multiplex PCR method, we applied a limit of detection assay and random reference material analysis. We also monitored the unintentional environmental release of GM alfalfa in South Korea by performing the multiplex PCR analysis with 91 feral alfalfa specimens collected from 2000 to 2018. Our methodology is a sensitive, simple, quick, and inexpensive tool for detecting and identifying three GM alfalfa varieties.

• Journal of Dairy Science and Biotechnology
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• 제29권2호
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• pp.43-49
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• 2011

The increasing number of analytes in concern and the alarming health and environmental consequences have required effective means of monitoring for safety control. Biosensors offer advantages as alternatives to conventional analytical methods because of their inherent specificity, simplicity, and quick response. Colorimetric biosensor, one of biosensor group, is one of the easiest and the most convenient methods because detection can be done using naked eye. Recently, a novel method for rapid detection and read-out of specific immunoassays with naked eye using polydiacetylene (PDA) was developed. Polydiacetylene has recently been in the limelight as a transducing materials because of its special features that allow optical transduction of sensory signals and inherent simplicity and ease of use in supramolecular chemistry. Various forms of PDA are used as a sensor platform for detection of various biological analytes such as viruses, DNA, proteins, bacteria and hazardous molecules.

• 대한의생명과학회지
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• 제29권3호
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• pp.109-120
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• 2023

Recently, the explosive increase of carbapenemase-producing Enterobacterales (CPE) in the worldwide poses a serious threat. The purpose of this study is to investigate epidemiology, detection, and treatment of CPE. Three main carbapenemase are reported worldwide, which were KPC, NDM, and OXA-48-like. KPC type are mostly found in USA, China, Europe, and Latin America. NDM type are mostly found in South Asia. OXA-48-like are often seen in the Mediterranean and Northern Africa. In Korea, CPE have increased explosively since 2015. In 2021, 18,099 CPE were isolated, which were Klebsiella pneumoniae, Escherichia coli, and Enterobacter cloacae in order. The CPE genotype was distributed with KPC, NDM, OXA type in order. Phenotypic detection methods include carbapenemase production tests (CPT) and differential tests of CPE. CPTs include modified Hodge test, modified carbapenem inactivation method (mCIM), Carba NP test, among which mCIM is the most widely used due to easy accessibility and accuracy. A lot of genotypic methods are being done for quick results, and commercialized kits using multiplex real-time PCR and microarray are widely used. Colistin and tigecycline are used as the first line of CPE treatment and are used in combination with second line drugs such as meropenem and fosfomycin.

• Molecules and Cells
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• 제39권11호
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• pp.807-813
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• 2016

Escherichia coli are important indicator organisms, used routinely for the monitoring of water and food safety. For quick, sensitive and real-time detection of E. coli we developed a 2'F modified RNA aptamer Ec3, by Cell-SELEX. The 31 nucleotide truncated Ec3 demonstrated improved binding and low nano-molar affinity to E. coli. The aptamer developed by us out-performs the commercial antibody and aptamer used for E. coli detection. Ec3(31) aptamer based E. coli detection was done using three different detection formats and the assay sensitivities were determined. Conventional Ec3(31)-biotin-streptavidin magnetic separation could detect E. coli with a limit of detection of $1.3{\times}10^6CFU/ml$. Although, optical analytic technique, biolayer interferometry, did not improve the sensitivity of detection for whole cells, a very significant improvement in the detection was seen with the E. coli cell lysate ($5{\times}10^4CFU/ml$). Finally we developed Electrochemical Impedance Spectroscopy (EIS) gap capacitance biosensor that has detection limits of $2{\times}10^4CFU/mL$ of E. coli cells, without any labeling and signal amplification techniques. We believe that our developed method can step towards more complex and real sample application.

• Food Science and Biotechnology
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• 제17권1호
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• pp.15-19
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• 2008

This research was conducted to develop a quick and sensitive method of detecting carbamate residues using the immobilization of antibody-antigen interactions with surface plasmon resonance (SPR). We have used commercialized surface plasmon resonance equipment (Biacore 3000). The antibody used for the immunoassay was specific for glutathione-s-transferase (GST) and the antigens included several carbamate pesticides (carbofuran, carbaryl, and benfuracarb). When antigens were applied to the protein GST, the detection limit was 2 ng/mL of carbamate pesticide. The fabricated protein GST maintained its activity for over 200 measurements. Thus we determined that the SPR biosensors could detect the specific reversible binding of a reactant in solution to a binding partner immobilized on the surface of the sensor and allow real-time detection and monitoring.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2000년도 추계종합학술대회 논문집(1)
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• pp.449-452
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• 2000

The economical detection of dual-tone multifrequency(DTMF) signals is an important factor when developing cost-effective telecommunication equipment. Each channel has independently a DTMF receiver, and it informs the detected signal to processors. This paper analyze the power spectra and evaluate the performance of DTMF receiver by using the quick Fourier transform(QFT) algorithm. As experimental results, it show the improved performance to the DTMF receivers and reduce memory waste and process the real-time.