• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.12
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• pp.427-436
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• 2016

Due to disasters such as earthquakes and landslides in urban areas, persons have been buried inside collapsed buildings and structures. Rescuers have mainly utilized detection equipment by applying sound, video and electric waves, but these are expensive and due to the directional approaches onto the collapsed site, secondary collapse risk can arise. In addition, due to poor utilization of such equipment, new human detection technology with quick and high reliability has not been utilized. To address these issues, this study develops a wireless signal-based human detection module that can be loaded into an Unmanned Aerial Vehicle (UAV). The human detection module searches for the 3D location for buried persons by collecting Wi-Fi signal and barometer sensors data transmitted from the mobile phones. This module can gain diverse information from mobile phones for buried persons in real time. We present a development framework of the module that provides 3D location data with more reliable information by delivering the collected data into a local computer in the ground. This study verified the application feasibility of the developed module in a real collapsed area. Therefore, it is expected that these results can be used as a core technology for the quick detection of buried persons' location and for relieving them after disasters that induce building collapses.

• BMB Reports
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• v.37 no.5
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• pp.546-551
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• 2004

The increasing pace of development in molecular biology during the last decade has had a direct effect on mass testing and diagnostic applications, including blood screening. We report the model Microarray that has been developed for Hepatitis B virus (HBV) and Hepatitis D virus (HDV) detection. The specific primer pairs of PCR were designed using the Primer Premier 5.00 program according to the conserved regions of HBV and HDV. PCR fragments were purified and cloned into pMD18-T vectors. The recombinant plasmids were extracted from positive clones and the target gene fragments were sequenced. The DNA microarray was prepared by robotically spotting PCR products onto the surface of glass slides. Sequences were aligned, and the results obtained showed that the products of PCR amplification were the required specific gene fragments of HBV, and HDV. Samples were labeled by Restriction Display PCR (RD-PCR). Gene chip hybridizing signals showed that the specificity and sensitivity required for HBV and HDV detection were satisfied. Using PCR amplified products to construct gene chips for the simultaneous clinical diagnosis of HBV and HDV resulted in a quick, simple, and effective method. We conclude that the DNA microarray assay system might be useful as a diagnostic technique in the clinical laboratory. Further applications of RD-PCR for the sample labeling could speed up microarray multi-virus detection.

• Proceedings of the Korean Information Science Society Conference
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• 2011.06c
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• pp.405-408
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• 2011

In this paper, we propose a robot remote control system based on mouth tracking. The main idea behind the work is to help disabled people who cannot operate a joystick or keyboard to control a robot with their hands. The mouth detection method in this paper is mainly based on the Adaboost feature detection approach. By using the proposed new Haar-like features for detecting the corner of mouth, the speed and accuracy of detection are improved. Combined with the Kalman filter, a continuous and accurate mouth tracking has been achieved. Meanwhile, the gripping commands of the robot manipulator were also achieved by the recognition of the user.s mouth shape, such as 'pout mouth' or 'grin mouth'. To assess the validity of the method, a mouth detection experiment and a robot cargo transport experiment were applied. The result indicated that the system can realize a quick and accurate mouse tracking; and the operation of the robot worked successfully in moving and bringing back items.

• Journal of Broadcast Engineering
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• v.21 no.2
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• pp.268-271
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• 2016

In video sequences, foreground object detection being composed of a background model and a background subtraction is an important part of diverse computer vision applications. However, object detection might fail in sudden illumination changes. In this letter, an illumination-robust background detection is proposed to address this problem. The method can provide quick adaption to current illumination condition using two background models with different adaption rates. Since the proposed method is a non-parametric approach, experimental results show that the proposed algorithm outperforms several state-of-art non-parametric approaches and provides low computational cost.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.1 no.1
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• pp.83-89
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• 2020

Methods for detecting the presence of genetically modified (GM) crops are evolving to comply with legislation and to enhance monitoring by biotechnology companies and regulators. In order to cover a broad range of detection methods for a new GM crop, conventional multiplex PCR methods are required. Based on the genetic information on three GM alfalfa varieties (J101, J163, and KK179), which were recently approved in South Korea, we developed a fast, reliable, and highly specific multiplex polymerase chain reaction (PCR) method with basic PCR equipment and inexpensive reagents. To validate and verify the newly developed multiplex PCR method, we applied a limit of detection assay and random reference material analysis. We also monitored the unintentional environmental release of GM alfalfa in South Korea by performing the multiplex PCR analysis with 91 feral alfalfa specimens collected from 2000 to 2018. Our methodology is a sensitive, simple, quick, and inexpensive tool for detecting and identifying three GM alfalfa varieties.

• Journal of Dairy Science and Biotechnology
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• v.29 no.2
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• pp.43-49
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• 2011

The increasing number of analytes in concern and the alarming health and environmental consequences have required effective means of monitoring for safety control. Biosensors offer advantages as alternatives to conventional analytical methods because of their inherent specificity, simplicity, and quick response. Colorimetric biosensor, one of biosensor group, is one of the easiest and the most convenient methods because detection can be done using naked eye. Recently, a novel method for rapid detection and read-out of specific immunoassays with naked eye using polydiacetylene (PDA) was developed. Polydiacetylene has recently been in the limelight as a transducing materials because of its special features that allow optical transduction of sensory signals and inherent simplicity and ease of use in supramolecular chemistry. Various forms of PDA are used as a sensor platform for detection of various biological analytes such as viruses, DNA, proteins, bacteria and hazardous molecules.

• Biomedical Science Letters
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• v.29 no.3
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• pp.109-120
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• 2023

Recently, the explosive increase of carbapenemase-producing Enterobacterales (CPE) in the worldwide poses a serious threat. The purpose of this study is to investigate epidemiology, detection, and treatment of CPE. Three main carbapenemase are reported worldwide, which were KPC, NDM, and OXA-48-like. KPC type are mostly found in USA, China, Europe, and Latin America. NDM type are mostly found in South Asia. OXA-48-like are often seen in the Mediterranean and Northern Africa. In Korea, CPE have increased explosively since 2015. In 2021, 18,099 CPE were isolated, which were Klebsiella pneumoniae, Escherichia coli, and Enterobacter cloacae in order. The CPE genotype was distributed with KPC, NDM, OXA type in order. Phenotypic detection methods include carbapenemase production tests (CPT) and differential tests of CPE. CPTs include modified Hodge test, modified carbapenem inactivation method (mCIM), Carba NP test, among which mCIM is the most widely used due to easy accessibility and accuracy. A lot of genotypic methods are being done for quick results, and commercialized kits using multiplex real-time PCR and microarray are widely used. Colistin and tigecycline are used as the first line of CPE treatment and are used in combination with second line drugs such as meropenem and fosfomycin.

• Molecules and Cells
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• v.39 no.11
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• pp.807-813
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• 2016

Escherichia coli are important indicator organisms, used routinely for the monitoring of water and food safety. For quick, sensitive and real-time detection of E. coli we developed a 2'F modified RNA aptamer Ec3, by Cell-SELEX. The 31 nucleotide truncated Ec3 demonstrated improved binding and low nano-molar affinity to E. coli. The aptamer developed by us out-performs the commercial antibody and aptamer used for E. coli detection. Ec3(31) aptamer based E. coli detection was done using three different detection formats and the assay sensitivities were determined. Conventional Ec3(31)-biotin-streptavidin magnetic separation could detect E. coli with a limit of detection of $1.3{\times}10^6CFU/ml$. Although, optical analytic technique, biolayer interferometry, did not improve the sensitivity of detection for whole cells, a very significant improvement in the detection was seen with the E. coli cell lysate ($5{\times}10^4CFU/ml$). Finally we developed Electrochemical Impedance Spectroscopy (EIS) gap capacitance biosensor that has detection limits of $2{\times}10^4CFU/mL$ of E. coli cells, without any labeling and signal amplification techniques. We believe that our developed method can step towards more complex and real sample application.

• Food Science and Biotechnology
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• v.17 no.1
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• pp.15-19
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• 2008

This research was conducted to develop a quick and sensitive method of detecting carbamate residues using the immobilization of antibody-antigen interactions with surface plasmon resonance (SPR). We have used commercialized surface plasmon resonance equipment (Biacore 3000). The antibody used for the immunoassay was specific for glutathione-s-transferase (GST) and the antigens included several carbamate pesticides (carbofuran, carbaryl, and benfuracarb). When antigens were applied to the protein GST, the detection limit was 2 ng/mL of carbamate pesticide. The fabricated protein GST maintained its activity for over 200 measurements. Thus we determined that the SPR biosensors could detect the specific reversible binding of a reactant in solution to a binding partner immobilized on the surface of the sensor and allow real-time detection and monitoring.

• Proceedings of the IEEK Conference
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• 2000.11a
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• pp.449-452
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• 2000

The economical detection of dual-tone multifrequency(DTMF) signals is an important factor when developing cost-effective telecommunication equipment. Each channel has independently a DTMF receiver, and it informs the detected signal to processors. This paper analyze the power spectra and evaluate the performance of DTMF receiver by using the quick Fourier transform(QFT) algorithm. As experimental results, it show the improved performance to the DTMF receivers and reduce memory waste and process the real-time.